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Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus.

Abstract：

:Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybridization with non-radioactive probes and hybridization with radiolabelled probes were 2 ng, 0.2 ng, 0.02 ng of purified virus, respectively. Dot hybridization with non-radioactive probes could be performed on crude infected beet root extracts, thus providing a useful tool for monitoring BNYVV infection and for routine testing in plant breeding programs.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Sakamoto H,Lemaire O,Merdinoglu D,Guesdon JL

doi

10.1016/0890-8508(89)90026-1

subject

Has Abstract

pub_date

1989-06-01 00:00:00

pages

159-66

issue

2

eissn

0890-8508

issn

1096-1194

pii

0890-8508(89)90026-1

journal_volume

3

pub_type

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