Abstract:
:The flagellin genes of four Burkholderia pseudomallei and two Burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (PCR)-based isolation method using the same pair of primers. The PCR-amplification products of the isolates showed a single band of about 1.1 kb, which is similar to a type II B. cepacia flagellin reported previously. In order to distinguish these two species based on the flagellin PCR-amplified products, Pst I and Xho I restriction endonuclease analysis was performed. The results suggest that there is sufficient diversity within the flagellin sequences of the closely related Burkholderia species, B. pseudomallei and B. cepacia, to enable flagellin-type identification on the basis of the pattern of restriction fragments. In addition, the flagellin gene should be of considerable use as a genetic marker for clinical identification of these organisms.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Tungpradabkul S,Wajanarogana S,Tunpiboonsak S,Panyim Sdoi
10.1006/mcpr.1999.0221subject
Has Abstractpub_date
1999-04-01 00:00:00pages
99-105issue
2eissn
0890-8508issn
1096-1194pii
S0890-8508(99)90221-9journal_volume
13pub_type
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