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Alternate PCR assays for screening of JH1 mutation associated with embryonic death in Jersey cattle.

Abstract：

:Jersey haplotype (JH) 1, a stop-gain lethal mutation in the CWC15 gene, causes embryonic losses in Jersey cattle. Two PCR based assays using Amplification Refractory Mutation System (T-ARMS-PCR) and restriction fragment length polymorphism (PCR-RFLP) were developed for screening of the JH1 in cattle. During the screening, seven among 30 Indian Jersey bulls were identified as carriers of the mutant JH1 allele, the first time in the country. These PCR assays are economical, rapid and accurate; and can be used separately or in combination for screening and cross-validation of the JH1 carriers in Jersey cattle.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Kumar A,Gupta ID,Mohan G,M R V,D RK,S J,Kataria RS,Niranjan SK

doi

10.1016/j.mcp.2020.101688

subject

Has Abstract

pub_date

2020-12-03 00:00:00

pages

101688

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(20)30577-6

journal_volume

55

pub_type

杂志文章

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