Abstract:
:Jersey haplotype (JH) 1, a stop-gain lethal mutation in the CWC15 gene, causes embryonic losses in Jersey cattle. Two PCR based assays using Amplification Refractory Mutation System (T-ARMS-PCR) and restriction fragment length polymorphism (PCR-RFLP) were developed for screening of the JH1 in cattle. During the screening, seven among 30 Indian Jersey bulls were identified as carriers of the mutant JH1 allele, the first time in the country. These PCR assays are economical, rapid and accurate; and can be used separately or in combination for screening and cross-validation of the JH1 carriers in Jersey cattle.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Kumar A,Gupta ID,Mohan G,M R V,D RK,S J,Kataria RS,Niranjan SKdoi
10.1016/j.mcp.2020.101688subject
Has Abstractpub_date
2020-12-03 00:00:00pages
101688eissn
0890-8508issn
1096-1194pii
S0890-8508(20)30577-6journal_volume
55pub_type
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
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journal_title:Molecular and cellular probes
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doi:10.1016/j.mcp.2008.09.003
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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abstract::The first report of pertactin-negative variants of Bordetella pertussis in the United States has raised questions about the role of acellular pertussis vaccines in the recent increase of pertussis cases. Our laboratory utilized a sequence-based method to identify mutations in the pertactin gene responsible for these v...
journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2013.12.003
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doi:10.1016/j.mcp.2020.101607
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1006/mcpr.1993.1017
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journal_title:Molecular and cellular probes
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pub_type: 杂志文章
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2019.04.001
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2009.10.001
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