当前位置： SCI文献检索 > MOLECULAR AND CELLULAR PROBES期刊下所有文献 > Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.

Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.

Abstract：

:A simple dual-label hybridization test for normal and mutant cystic fibrosis (CF) alleles is described. The assay is based on time-resolved fluorometry (TRF), which allows the simultaneous detection of DNA probes labelled with different lanthanides from one hybridization reaction. DNA was liberated from dried blood disks, normally used in neonatal screening programmes, by boiling in alkaline solution. A 138 bp region including the site of deletion, delta F-508, which is present on about 70% of cystic fibrosis chromosomes, was amplified using the polymerase chain reaction (PCR). The presence or absence of normal and mutant alleles was then determined in a solution hybridization using allele specific oligonucleotide probes labelled either with europium (Eu) or with samarium (Sm) chelates. A common biotinylated probe was used for binding the hybrids onto microtitration wells coated with streptavidin. Some 5 x 10(7) molecules of the normal allele (Eu) and 5 x 10(8) molecules of the mutant allele (Sm) could be detected simultaneously in a single hybridization reaction. The assay was simple to perform and made it possible to reduce the number of hybridizations needed to interpret the sample as being normal, carrier or mutant with regard to the mutation, delta F-508.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Iitiä A,Liukkonen L,Siitari H

doi

10.1016/0890-8508(92)90047-2

subject

Has Abstract

pub_date

1992-12-01 00:00:00

pages

505-12

issue

6

eissn

0890-8508

issn

1096-1194

journal_volume

6

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR PROBES文献大全
  • Aptamers, the bivalent agents as probes and therapies for coronavirus infections: A systematic review.

    abstract：:The recently known coronavirus, SARS-CoV-2, has turn into the greatest global health challenge, affecting a large number of societies. The lack of specific treatment and gold-standard diagnostic system has made the situation more complicated. Efforts have led to production of several diagnostic kits that are associate...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.mcp.2020.101636

    authors： Torabi R,Ranjbar R,Halaji M,Heiat M

    更新日期：2020-10-01 00:00:00

  • Comparison of multiplex PCR, PCR-ELISA and fluorogenic 5' nuclease PCR assays for detection of plasmid-bearing virulent Yersinia enterocolitica in swine feces.

    abstract：:Swine are implicated as the principal animal reservoir for plasmid-bearing Yersinia enterocolitica (YEP(+)) strains that are pathogenic to humans. To evaluate the utility of the PCR for detection of YEP(+) strains in naturally-contaminated pig feces, samples were first enriched in Irgasan ticarcillin potassium chlorat...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2002.0408

    authors： Bhaduri S

    更新日期：2002-06-01 00:00:00

  • Characterization of four microsatellites in an Italian population and their application to paternity testing.

    abstract：:Microsatellites have recently been used for linkage analysis of genetic diseases and for DNA fingerprinting in forensic medicine. In the present study the heterozygosity, PIC values and allele distributions of four microsatellites, D8S85, D8S88, D5S346 and D7S460, in an Italian population have been investigated. After...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1996.0021

    authors： Sirchia SM,Garagiola I,De Andreis C,Gazzoli I,Gramegna M,Colucci G

    更新日期：1996-04-01 00:00:00

  • Rapid PCR using nested primers of the 16S rRNA and the hippuricase (hip O) genes to detect Campylobacter jejuni and Campylobacter coli in environmental samples.

    abstract：:Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2002.0434

    authors： Bang DD,Wedderkopp A,Pedersen K,Madsen M

    更新日期：2002-10-01 00:00:00

  • A new method for species identification and differentiation of Mycobacterium chelonae complex based on amplified hsp65 restriction analysis (AHSPRA).

    abstract：:Members of the Mycobacterium chelonae complex (MCC), namely M. chelonae, Mycobacterium abscessus and Mycobacterium immunogenum, have been implicated in nosocomial infections and occupational respiratory illnesses like hypersensitivity pneumonitis (HP) associated with contaminated metalworking fluid (MWF) exposures. Cl...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2004.09.007

    authors： Selvaraju SB,Khan IU,Yadav JS

    更新日期：2005-04-01 00:00:00

  • Direct identification of Pseudomonas aeruginosa from blood culture bottles by PCR-enzyme linked immunosorbent assay using oprI gene specific primers.

    abstract：:A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for direct identification of Pseudomonas aeruginosa from positive BACTEC blood culture bottles. PCR primers were designed to target a 249 bp sequence of the oprI gene in P. aeruginosa. Biotin-labeled probe (PA3) targeted to the species-specific motif we...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2005.07.005

    authors： Kurupati P,Kumarasinghe G,Laa Poh C

    更新日期：2005-12-01 00:00:00

  • Specific detection of Campylobacter concisus by PCR amplification of 23S rDNA areas.

    abstract：:The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. conc...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(95)90114-0

    authors： Bastyns K,Chapelle S,Vandamme P,Goossens H,De Wachter R

    更新日期：1995-08-01 00:00:00

  • Quantitative PCR for the diagnosis of cutaneous leishmaniasis from formalin-fixed and paraffin-embedded skin sections.

    abstract：:The present report describes a real-time PCR-based procedure to reliably determine the quantity of Leishmania amastigotes in relation to the amount of host tissue in histological skin sections from canine and equine cases of cutaneous leishmaniasis. The novel diagnostic Leishmania-PCR has a detection limit of <0.02 am...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2015.09.008

    authors： Müller N,Hentrich B,Frey CF,Welle M

    更新日期：2015-12-01 00:00:00

  • High-throughput qualitative multiplex 5' nuclease assay using post-only PCR analysis.

    abstract：:Homogenous fluorescence PCR assays offer distinct advantages for qualitative testing and are gaining immense popularity in fields like diagnostic microbiology. To meet the demand of high-volume laboratories, we developed a protocol for qualitative multiplex 5' nuclease assays using post-only PCR analysis. This novel a...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2005.06.001

    authors： Antonishyn NA,McDonald RR,Chan EL,Horsman GB

    更新日期：2005-10-01 00:00:00

  • Selection of stable reference genes for gene expression analysis in sweet potato (Ipomoea batatas L.).

    abstract：:Gene expression analysis is one of the most common and important studies in biology and biomedicine. No matter for traditional blotting analysis or currently commonly used PCR strategy, all need a stable reference gene for normalizing the gene expression. To screen and select housekeeping genes as the most stable refe...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101610

    authors： Yu J,Su Y,Sun J,Liu J,Li Z,Zhang B

    更新日期：2020-10-01 00:00:00

  • A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter.

    abstract：:In a previous study, we reported the performance of a PCR assay amplifying 287-bp of the 16S rRNA gene of thermo-tolerant Campylobacter (C. jejuni, C. lari, C. coli) through an international ring-trial involving 12 participating laboratories. Based on the validated set of primers, a LightCycler real-time PCR assay (LC...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2004.04.005

    authors： Perelle S,Josefsen M,Hoorfar J,Dilasser F,Grout J,Fach P

    更新日期：2004-10-01 00:00:00

  • Tracking the extramedullary PML-RARα-positive cell reservoirs in a preclinical model: biomarker of long-term drug efficacy.

    abstract：:Using an acute promyelocytic leukemia (APL) preclinical model, we show that oncogene-specific PCR (Polymerase Chain Reaction)-based assays allow to evaluate the efficacy of immunotherapy combining all-trans retinoic acid (ATRA) and a DNA-based vaccine targeting the promyelocytic leukemia-retinoic acid receptor alpha (...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2012.08.001

    authors： Pokorna K,Le Pogam C,Chopin M,Balitrand N,Reboul M,Cassinat B,Chomienne C,Padua RA,Pla M

    更新日期：2013-02-01 00:00:00

  • Rapid PCR-based delineation of the porcine nodular worms, Oesophagostomum dentatum and O. quadrispinulatum.

    abstract：:At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1997.0097

    authors： Newton LA,Chilton NB,Monti JR,Bjørn H,Várady M,Christensen CM,Gasser RB

    更新日期：1997-04-01 00:00:00

  • Immunoblot and enzyme-linked immunosorbent assays of Campylobacter major outer-membrane protein and application to the differentiation of Campylobacter species.

    abstract：:The major outer-membrane protein (MOMP) from Campylobacter jejuni NCTC 11168 was purified by solubilization in Triton X-100. Whole-cell proteins of Campylobacter species and the MOMP were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linke...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(87)90038-7

    authors： Taylor DE,Chang N

    更新日期：1987-09-01 00:00:00

  • Up-regulated miR-374a-3p relieves lipopolysaccharides induced injury in CHON-001 cells via regulating Wingless-type MMTV integration site family member 5B.

    abstract：BACKGROUND:Osteoarthritis (OA) is a frequent and incurable joint disease, inducing significant pain and seriously threatening to human health. It has been reported that microRNAs (miRNAs) play crucial roles on cancers and inflammatory diseases via cooperating with genes. However, the effect of miR-374a-3p/Wingless-type...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101541

    authors： Shi FL,Ren LX

    更新日期：2020-06-01 00:00:00

  • Transcriptional profiles of regulatory and virulence factors of Staphylococcus aureus of bovine origin: oxygen impact and strain-to-strain variations.

    abstract：:Staphylococcus aureus is responsible for a large panel of infections in humans and animals. In cows, S. aureus provokes chronic intramammary infections. Little information is available about the regulation of virulence factors in bovine isolates. Moreover, oxygenation, which is low in an inflamed mammary gland, could ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2005.01.002

    authors： Ster C,Gilbert FB,Cochard T,Poutrel B

    更新日期：2005-08-01 00:00:00

  • The use of real-time polymerase chain reaction with high resolution melting (real-time PCR-HRM) analysis for the detection and discrimination of nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus.

    abstract：:The real-time PCR-HRM analysis was developed for the detection and discrimination of the quarantine nematode Bursaphelenchus xylophilus and Bursaphelenchus mucronatus. A set of primers was designed to target the ITS region of rDNA. The results have demonstrated that this analysis is a valuable tool for differentiation...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2016.02.003

    authors： Filipiak A,Hasiów-Jaroszewska B

    更新日期：2016-04-01 00:00:00

  • Simultaneous detection of erythromycin-resistant methylase genes ermA and ermC from Staphylococcus spp. by multiplex-PCR.

    abstract：:A comparative analysis of the two most dominant erythromycin-resistance determinant genes in Staphylococcus sppnamely, the ermA and ermC genes, was carried out. Sixty erythromycin-resistant strains of Staphylococcus spp. were tested, of which 24 were avian and 36 were clinical isolates. Our results indicated the preva...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1999.0265

    authors： Khan SA,Nawaz MS,Khan AA,Cerniglia CE

    更新日期：1999-10-01 00:00:00

  • Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus.

    abstract：:Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA L...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2017.03.005

    authors： Yang Y,Qin X,Zhang W,Li Z,Zhang S,Li Y,Zhang Z

    更新日期：2017-06-01 00:00:00

  • Molecular diagnostic tests to predict the risk of progressive multifocal leukoencephalopathy in natalizumab-treated multiple sclerosis patients.

    abstract：:Natalizumab is a humanized monoclonal antibody against the alpha4 chain of the alpha4beta1 and alpha4beta7 integrin heterodimers used with high effectiveness in the treatment of multiple sclerosis. The use of this drug can unfortunately be associated with the onset of progressive multifocal leukoencephalopathy, a poss...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章,评审

    doi：10.1016/j.mcp.2014.11.007

    authors： Rossi F,Newsome SD,Viscidi R

    更新日期：2015-02-01 00:00:00

  • Construction of an internal control for the detection of Chlamydia pneumoniae by PCR.

    abstract：:For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1998.0180

    authors： Ursi D,Ieven M,Van Bever HP,Goossens H

    更新日期：1998-08-01 00:00:00

  • Detection and molecular typing of Campylobacter jejuni in fecal samples by polymerase chain reaction.

    abstract：:In order to determine whether polymerase chain reaction (PCR) could be used to detect Campylobacter jejuni directly in stool samples, DNA from 66 frozen culture positive and negative fecal samples was purified by column chromatography. The flaA gene was amplified using primers directed against the conserved 5' and 3' ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1996.0011

    authors： Waegel A,Nachamkin I

    更新日期：1996-04-01 00:00:00

  • Microduplication of 10q26.3 in a Chinese hypertriglyceridemia patient.

    abstract：:Hypertriglyceridemia (HTG) plays an important role in the development and progression of atherosclerosis. It is inherited in an autosomal dominant pattern with a frequency of approximately 1:1,000,000 worldwide. Previous study has demonstrated that more than six genes underlie this disorder. In addition, copy number v...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2017.11.002

    authors： Li JJ,Chen YQ,Fan LL,Jin JY,Guo S,Xiang R

    更新日期：2018-02-01 00:00:00

  • Evaluation of a DNA probe of plasmid origin for the detection of Chlamydia trachomatis in cultures and clinical specimens.

    abstract：:This study evaluates five cryptic plasmid-derived DNA probes in a 4-h slot-blot hybridization assay for the detection of Chlamydia trachomatis in cultures and clinical specimens. The probes, consisting of either the entire cloned 7.5 kbp cryptic plasmid pSE8 or one of four Hin dIII/Eco RI fragments measuring 710, 1055...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(05)80013-1

    authors： Cano RJ,Murrieta CM,Spaulding DC,Pascual A

    更新日期：1991-12-01 00:00:00

  • Cathepsin L-like genes of Trypanosoma vivax from Africa and South America--characterization, relationships and diagnostic implications.

    abstract：:We characterized sequences from genes encoding cathepsin L-like (CatL-like) cysteine proteases from African and South American isolates of Trypanosoma vivax and T. vivax-like organisms, and evaluated their suitability as genetic markers for population structure analysis and diagnosis. Phylogenetic analysis of sequence...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2008.11.003

    authors： Cortez AP,Rodrigues AC,Garcia HA,Neves L,Batista JS,Bengaly Z,Paiva F,Teixeira MM

    更新日期：2009-02-01 00:00:00

  • HyBeacon probes: a new tool for DNA sequence detection and allele discrimination.

    abstract：:Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2001.0384

    authors： French DJ,Archard CL,Brown T,McDowell DG

    更新日期：2001-12-01 00:00:00

  • Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

    abstract：:Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1994.1035

    authors： Axton RA,Brock DJ

    更新日期：1994-06-01 00:00:00

  • A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR.

    abstract：:Species identification of Mycobacterium tuberculosis remains a cumbersome process. We have developed a simple method for treating clinical samples which permits direct polymerase chain reaction (PCR) amplification of mycobacterial target DNA without organic extraction. Samples were boiled for 30 min in TE-Triton, then...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(06)80011-3

    authors： Sritharan V,Barker RH Jr

    更新日期：1991-10-01 00:00:00

  • Fluorescence-based DNA minisequence analysis for detection of known single-base changes in genomic DNA.

    abstract：:We describe a rapid, automated method for direct detection of known single-base changes in genomic DNA. Fluorescence-based DNA minisequence analysis is employed in a template-dependent reaction which involves a single nucleotide extension of an oligonucleotide primer by the correct fluorescently-tagged dideoxynucleoti...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1995.0027

    authors： Kobayashi M,Rappaport E,Blasband A,Semeraro A,Sartore M,Surrey S,Fortina P

    更新日期：1995-06-01 00:00:00

  • No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls.

    abstract：:New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2011.01.005

    authors： Sabunciyan S,Mandelberg N,Rabkin CS,Yolken R,Viscidi R

    更新日期：2011-04-01 00:00:00