Development and evaluation of the polymerase chain reaction method for diagnosis of Mycoplasma gallisepticum infection in chickens.

Abstract:

:A polymerase chain reaction (PCR) method specific for Mycoplasma gallisepticum (MG) was evaluated. The PCR method was found to detect as few as two colour changing units (CCU) of MG and did not give false positive reactions with other avian mycoplasmas. In chickens inoculated with either MG or Mycoplasma synoviae (MS), the PCR method was found to closely correlate with MG culture reisolation methods in chicken intranasally inoculated with MG. All chickens inoculated with MS tested negative using the MG PCR method.

journal_name

Mol Cell Probes

authors

Slavik MF,Wang RF,Cao WW

doi

10.1006/mcpr.1993.1068

subject

Has Abstract

pub_date

1993-12-01 00:00:00

pages

459-63

issue

6

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(83)71068-6

journal_volume

7

pub_type

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