Development of a p28-based PCR assay for Ehrlichia chaffeensis.

abstract：:The polymerase spiral reaction (PSR), a novel isothermal method for targeted DNA amplification, was effectively applied to detect Salmonella in artificially spiked pork. The specificity of the developed PSR was tested using 16 Salmonella and 15 non-Salmonella strains. The PSR assay was 10-fold more sensitive than conv...

journal_title：Molecular and cellular probes

authors： Momin KM,Milton AAP,Ghatak S,Thomas SC,Priya GB,Das S,Shakuntala I,Sanjukta R,Puro KU,Sen A

更新日期：2020-04-01 00:00:00

abstract：:In the present study, multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for simultaneously detection of six major shrimp viruses including yellow-head virus (YHV), white spot syndrome virus (WSSV), Taura syndrome virus (TSV), hepatopancreatic parvovirus (HPV), infectious hypodermal and ...

journal_title：Molecular and cellular probes

authors： Khawsak P,Deesukon W,Chaivisuthangkura P,Sukhumsirichart W

更新日期：2008-06-01 00:00:00

abstract：:The major cause of first-trimester pregnancy loss is chromosomal abnormality, which could be detected by many methods. Conventional karyotyping based on chorionic villi (CV) culture is frequently used but may have limitations due to culture failure and selective growth of cells. In this study, we aimed to investigate ...

journal_title：Molecular and cellular probes

authors： Zhang Y,Lei Q,Liu J,Lin M,Luo L,Li T,Wang Q,Zhou C

更新日期：2020-06-01 00:00:00

abstract：:Holstein haplotype (HH) 1, 3 and 4 are lethal mutations, responsible for early embryonic losses in Holstein Friesian (HF) cattle, worldwide. Three PCR based assays - tetra Amplification Refractory Mutation System PCR, PCR primer induced restriction analysis and PCR-restriction fragment length polymorphism techniques f...

journal_title：Molecular and cellular probes

authors： Kumar A,Gupta ID,Mohan G,Vineeth MR,Ravi Kumar D,Jayakumar S,Niranjan SK

更新日期：2020-04-01 00:00:00

abstract：:The LUX [Light Upon eXtension] is a real-time detection system that can be used for the detection and quantification of pathogens nucleic acids. In this study we used a universal LUX approach, a variation of the LUX detection system, for identifying Orthopoxvirus nucleic acids in real time. This approach enables the d...

journal_title：Molecular and cellular probes

authors： Aitichou M,Javorschi S,Ibrahim MS

更新日期：2005-10-01 00:00:00

abstract：:Development of rapid amplification assays for the detection and identification of biological threat agents has become a focus of diagnostic efforts in recent years. The use of real-time PCR assays as diagnostic tools depends upon two critical processes. First, nucleic acid purification must provide template that is bo...

journal_title：Molecular and cellular probes

authors： Hartman LJ,Coyne SR,Norwood DA

更新日期：2005-02-01 00:00:00

abstract：:Serum lipid profile which is determined by genotype-phenotype relationship plays a significant role in the development of cardiovascular disease. Upstream stimulatory factor 1 (USF1), has been reported to be associated with serum lipid levels in different population, hence, this study investigated the association of v...

journal_title：Molecular and cellular probes

authors： Onadeko OT,Okunowo WO,Imaga NOA,Abdulrazaq MM,Onuminya OJ,Van-Lare TO,Nwosu M

更新日期：2020-12-08 00:00:00

abstract：:The unique ability of homopyrimidine peptide nucleic acid (PNA) to strand invade homopurine sites of duplex DNA offers a potential alternative to existing techniques for rapid detection of PCR products. From gel shift studies, PNA was found to specifically strand invade homopurine sites that had been incorporated into...

journal_title：Molecular and cellular probes

authors： Drewe LJ,Brightwell G,Hall EA

更新日期：2000-10-01 00:00:00

abstract：:The flagellin genes of four Burkholderia pseudomallei and two Burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (PCR)-based isolation method using the same pair of primers. The PCR-amplification products of the isolates showed a single band of about 1.1 kb, which is similar to a type I...

journal_title：Molecular and cellular probes

authors： Tungpradabkul S,Wajanarogana S,Tunpiboonsak S,Panyim S

更新日期：1999-04-01 00:00:00

abstract：:The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. conc...

journal_title：Molecular and cellular probes

authors： Bastyns K,Chapelle S,Vandamme P,Goossens H,De Wachter R

更新日期：1995-08-01 00:00:00

abstract：BACKGROUND:Osteoarthritis (OA) is a frequent and incurable joint disease, inducing significant pain and seriously threatening to human health. It has been reported that microRNAs (miRNAs) play crucial roles on cancers and inflammatory diseases via cooperating with genes. However, the effect of miR-374a-3p/Wingless-type...

journal_title：Molecular and cellular probes

authors： Shi FL,Ren LX

更新日期：2020-06-01 00:00:00

abstract：:Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasm...

journal_title：Molecular and cellular probes

authors： Wang H,Fadl AA,Khan MI

更新日期：1997-06-01 00:00:00

abstract：:The molecular tag vaccine against Brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. They can discriminate vaccinated animals and humans from those naturally infected. In this study, we constructed a gene deletion mutant strain, B. abortus S19 virB5 with a molecu...

journal_title：Molecular and cellular probes

authors： Tan W,Wang XR,Nie Y,Wang C,Cheng LQ,Wang XC,Zhang R,Yan GM

更新日期：2012-06-01 00:00:00

abstract：PURPOSE:This study aimed to analyze the relationships of long non-coding RNAs (lncRNAs) and protein-coding genes in lung squamous cell carcinoma (LUSC). METHODS:RNA-seq data of LUSC deposited in the TCGA database were used to identify differentially expressed protein-coding genes (DECGs) and differentially expressed l...

journal_title：Molecular and cellular probes

authors： Wu X,Ruan L,Yang Y,Mei Q

更新日期：2016-06-01 00:00:00

abstract：:For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

journal_title：Molecular and cellular probes

authors： Ursi D,Ieven M,Van Bever HP,Goossens H

更新日期：1998-08-01 00:00:00

abstract：:An oligonucleotide microarray was constructed for the rapid and sensitive molecular detection of antibiotic resistance determinants in Staphylococcus aureus. The array is equipped with oligonucleotide capture probes for the detection of 10 clinically and therapeutically relevant antibiotic resistance genes and -mutati...

journal_title：Molecular and cellular probes

authors： Strommenger B,Schmidt C,Werner G,Roessle-Lorch B,Bachmann TT,Witte W

更新日期：2007-06-01 00:00:00

abstract：:Swine are implicated as the principal animal reservoir for plasmid-bearing Yersinia enterocolitica (YEP(+)) strains that are pathogenic to humans. To evaluate the utility of the PCR for detection of YEP(+) strains in naturally-contaminated pig feces, samples were first enriched in Irgasan ticarcillin potassium chlorat...

journal_title：Molecular and cellular probes

authors： Bhaduri S

更新日期：2002-06-01 00:00:00

abstract：:Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is a highly contagious intestinal disease, resulting in substantial economic losses to the swine industry worldwide. In this study, three assays, namely a conventional reverse transcription-polymerase chain reaction (RT-PCR), a SYBR Green...

journal_title：Molecular and cellular probes

authors： Zhou X,Zhang T,Song D,Huang T,Peng Q,Chen Y,Li A,Zhang F,Wu Q,Ye Y,Tang Y

更新日期：2017-06-01 00:00:00

abstract：:Genomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentia...

journal_title：Molecular and cellular probes

authors： Baldan F,Passon N,Burra S,Demori E,Russo PD,Damante G

更新日期：2019-08-01 00:00:00

abstract：:We characterized sequences from genes encoding cathepsin L-like (CatL-like) cysteine proteases from African and South American isolates of Trypanosoma vivax and T. vivax-like organisms, and evaluated their suitability as genetic markers for population structure analysis and diagnosis. Phylogenetic analysis of sequence...

journal_title：Molecular and cellular probes

authors： Cortez AP,Rodrigues AC,Garcia HA,Neves L,Batista JS,Bengaly Z,Paiva F,Teixeira MM

更新日期：2009-02-01 00:00:00

abstract：:The complete nucleotide sequence of 85A antigen of Mycobacterium gordonae was determined. This gene encodes 339 amino acids, including 43 amino acids for the signal peptide, followed by a mature protein of 296 amino acids. A polymerase chain reaction (PCR) assay for the rapid detection of M. gordonae DNA using two pai...

journal_title：Molecular and cellular probes

authors： Dumonceaux M,Fauville Dufaux M,Ooms J,De Wit L,Sonck P,Content J

更新日期：1997-08-01 00:00:00

abstract：:This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

journal_title：Molecular and cellular probes

authors： Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

更新日期：2020-10-01 00:00:00

abstract：:A multiplex PCR for the simultaneous detection of some pathogenic genes of enteropathogenic, enterotoxigenic and verocytotoxin-producing Escherichia coli was developed. In this study primers found in literature as well as primers to the purpose designed were used. In this way, it was possible to generate specific frag...

journal_title：Molecular and cellular probes

authors： Bottero MT,Dalmasso A,Soglia D,Rosati S,Decastelli L,Civera T

更新日期：2004-08-01 00:00:00

abstract：:The identification and differentiation of the two variants of the ail gene, ailA from the more virulent American serotypes (08, 013a, 13b, 018, 020, 021) and ailNA from the less virulent non-American serotypes (03, 04, 05, 06, 09, 027 and 07, 8) was studied in a panel of 32 Yersinia enterocolitica human pathogenic iso...

journal_title：Molecular and cellular probes

authors： Sayada C,Picard B,Elion J,Krishnamoorthy R

更新日期：1994-06-01 00:00:00

abstract：:The human SNAIL is an important developmental protein involved in the formation of mesoderm and neural crest. The protein contains three classic and one atypical zinc-finger motif. The SNAI1 gene is composed of three exons. We have identified three SNPs in non-coding regions, two in the 5'UTR and one in intron 1, whic...

journal_title：Molecular and cellular probes

authors： Okajima K,Paznekas WA,Burstyn T,Jabs EW

更新日期：2001-02-01 00:00:00

abstract：:We describe a rapid, automated method for direct detection of known single-base changes in genomic DNA. Fluorescence-based DNA minisequence analysis is employed in a template-dependent reaction which involves a single nucleotide extension of an oligonucleotide primer by the correct fluorescently-tagged dideoxynucleoti...

journal_title：Molecular and cellular probes

authors： Kobayashi M,Rappaport E,Blasband A,Semeraro A,Sartore M,Surrey S,Fortina P

更新日期：1995-06-01 00:00:00

abstract：:Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myoc...

journal_title：Molecular and cellular probes

authors： Xia X,Liang Y,Zheng W,Lin D,Sun S

更新日期：2020-08-01 00:00:00

abstract：:The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

journal_title：Molecular and cellular probes

authors： Chaubert P,Burri N,Cousin P,Shaw P

更新日期：2001-06-01 00:00:00

abstract：:PCR detection and quantification of vaginal lactobacilli remains problematic because of the high level of genetic heterogeneity and taxonomic complexity within the genus Lactobacillus. The aim of the present study was to identify conserved sequences among the genomes of major species of vaginal lactobacilli that could...

journal_title：Molecular and cellular probes

authors： Demkin VV,Koshechkin SI,Slesarev A

更新日期：2017-04-01 00:00:00

abstract：:Sepsis is a common complication in infection, trauma, and surgery. Severe sepsis has been identified as the leading cause of death in patients suffering from noncardiovascular ailments in intensive care units. In the current study, we used lipopolysaccharide (LPS) to stimulate the mouse macrophage cell line RAW264.7, ...

journal_title：Molecular and cellular probes

authors： Yang Q,Cao K,Jin G,Zhang J

更新日期：2019-10-01 00:00:00