Specific detection of Campylobacter concisus by PCR amplification of 23S rDNA areas.

Abstract:

:The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. concisus, they are too laborious to be performed in routine settings. Hence a simple Campylobacter concisus-specific PCR assay was developed, based on a target sequence which comprises the most variable areas of 23S rDNA. The PCR assay was successfully evaluated on a broad selection of C. concisus strains and phylogenetically related bacteria.

journal_name

Mol Cell Probes

authors

Bastyns K,Chapelle S,Vandamme P,Goossens H,De Wachter R

doi

10.1016/s0890-8508(95)90114-0

subject

Has Abstract

pub_date

1995-08-01 00:00:00

pages

247-50

issue

4

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(95)90114-0

journal_volume

9

pub_type

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