Abstract：
:The major cause of first-trimester pregnancy loss is chromosomal abnormality, which could be detected by many methods. Conventional karyotyping based on chorionic villi (CV) culture is frequently used but may have limitations due to culture failure and selective growth of cells. In this study, we aimed to investigate the degree of mosaicism present in villi by a combination of three different methods, namely conventional karyotyping following culture, multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH), with a view to exploring the incidence of selective growth of mosaic CV cells during the process of culture for conventional karyotyping. CV samples were obtained from 207 patients with early spontaneous miscarriage (ESM). There were 56 (56/207, 27.1%) samples with mosaic chromosome detected by FISH based on four or five types of probes in this study. The incidence of selective growth of mosaic cells during the process of conventional karyotyping was 6.0% (11/183). In addition, we found that mosaic cell lines as low as 2% could grow and completely dominate the outcome of karyotyping results. The selective growth of a particular cell line during culture, whether euploidy or aneuploidy, could supress the diagnosis of mosaicism.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Zhang Y,Lei Q,Liu J,Lin M,Luo L,Li T,Wang Q,Zhou Cdoi
10.1016/j.mcp.2020.101532subject
Has Abstractpub_date
2020-06-01 00:00:00pages
101532eissn
0890-8508issn
1096-1194pii
S0890-8508(19)30396-2journal_volume
51pub_type
杂志文章abstract：:A beacon probe was designed to detect one of the two documented single nucleotide changes in IS481 target allele of Bordetella holmesii genome as compared to Bordetella pertussis. PCR amplified product targeting a region of IS481 in presence of the probe was subjected to a post-PCR hybridization and melting cycle. Hyb...
journal_title：Molecular and cellular probes
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journal_title：Molecular and cellular probes
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journal_title：Molecular and cellular probes
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/s0890-8508(95)90114-0
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journal_title：Molecular and cellular probes
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1995.0027
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2008.02.001
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2007.06.006
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journal_title：Molecular and cellular probes
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更新日期：2004-02-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
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更新日期：2015-02-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1996.0011
更新日期：1996-04-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2013.08.001
更新日期：2013-10-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2013.07.003
更新日期：2013-10-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1998.0201
更新日期：1998-12-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/0890-8508(88)90009-6
更新日期：1988-12-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/s0890-8508(03)00062-8
更新日期：2003-10-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.2001.0352
更新日期：2001-06-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2007.12.001
更新日期：2008-06-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2017.02.006
更新日期：2017-06-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
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journal_title：Molecular and cellular probes
pub_type： 杂志文章,评审
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更新日期：2016-02-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章,评审
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更新日期：1993-06-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2020.101601
更新日期：2020-10-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2010.07.002
更新日期：2010-12-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1998.0211
更新日期：1999-02-01 00:00:00
abstract：:The aim of the present study was to evaluate a mixed phase DNA hybridization assay for detection of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal aspirates from patients with suspected pertussis. Among 179 consecutive patients with own or parental suspicion of pertussis, the diagnosis was confirm...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/0890-8508(90)90021-q
更新日期：1990-08-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/s0890-8508(06)80011-3
更新日期：1991-10-01 00:00:00
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journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2016.03.007
更新日期：2016-06-01 00:00:00
abstract：:Analysis of single nucleotide polymorphisms by PCR with fluorescence resonance energy transfer (FRET) probes often can produce a result where the melting peak corresponding to perfectly matched sequence (A allele) has a smaller area than the peak corresponding to the allele with a mismatch (B allele). This imbalance c...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2004.03.003
更新日期：2004-08-01 00:00:00
abstract：:Accurate and timely diagnosis of acute bacterial meningitis is critical for antimicrobial treatment of patients. Although PCR-based methods have been widely used for the diagnosis of acute meningitis caused by bacterial pathogens, the main disadvantage of these methods is their high cost. This disadvantage has hampere...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2016.07.002
更新日期：2016-08-01 00:00:00
