Abstract:
:The human respiratory syncytial virus is a common respiratory pathogen in children. Improved diagnosis of the virus is dependent on the development of tools for the rapid detection and estimation of the viral loads. In the current study, RT-qPCR using TaqMan hydrolysis probe based on the F gene detection was developed to identify and quantify hRSV in clinical samples. The assay was validated by comparing the results with a commercially available RT-qPCR kit. The newly developed assay was sensitive in detecting hRSV positive samples (59/126) which were equivalent to those detected by the commercial kit (57/126) with a detection limit of 1 × 102 copies/mL. A high correlation was found between the results of the newly developed assay and the commercial one. It was concluded that the newly developed RT-qPCR assay can be used as a sensitive detection tool for hRSV-A.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Abdel-Moneim AS,Shehab GM,Alsulaimani AA,Al-Malky MIRdoi
10.1016/j.mcp.2017.02.006subject
Has Abstractpub_date
2017-06-01 00:00:00pages
16-19eissn
0890-8508issn
1096-1194pii
S0890-8508(17)30018-Xjournal_volume
33pub_type
杂志文章abstract::The ability to preserve and transport human cells in a stable medium over long distances is critical to collaborative efforts and the advancement of knowledge in the study of human disease. This is particularly important in the study of rare diseases. Recently, advancements in the understanding of renal ciliopathies h...
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