Rapid and cost effective genotyping method for polymorphisms in PPARG, PPARGC1 and TCF7L2 genes.


:Polymorphisms (rs1801282, rs8192678, rs7903146) of peroxisome proliferator-activated receptor gamma (PPARG), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PPARGC1A) and transcription factor 7-like 2 (TCF7L2) have recently been associated with different diseases, mainly type 2 diabetes. An assay using unlabeled probes and the LightCycler or Rotor-Gene instruments was developed for genotyping of these three polymorphisms. Asymmetric polymerase chain reaction was used, followed by melting analysis of the unlabeled probe/ssDNA amplicon duplex. Samples with the target genotypes were accurately detected and easily distinguishable. Thus, genotyping using unlabeled probes is a rapid, accurate and cost effective closed-tube method. These assays demonstrated 100% specificity and sensitivity for the identification of selected polymorphisms in PPARG, PPARGC1A and TCF7L2 genes.


Mol Cell Probes


Habalová V,Klimcáková L,Zidzik J,Tkác I




Has Abstract


2009-02-01 00:00:00














  • A simple PCR-based genotyping method for M105I mutation of alpha-SNAP enhances the study of early pathological changes in hyh phenotype.

    abstract::alpha-SNAP is an essential component of the protein machinery responsible for membrane fusion events in different cell types. The hyh (hydrocephalus with hop gait) mouse carries a missense mutation in Napa gene that results in a point mutation (M105I) in alpha-SNAP protein. Homozygous animals for the mutant allele hav...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bátiz LF,Roales-Buján R,Rodríguez-Pérez LM,Matas IM,Páez P,Roque M,Jiménez AJ,Ramos C,Pérez-Fígares JM

    更新日期:2009-12-01 00:00:00

  • Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus.

    abstract::Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybri...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sakamoto H,Lemaire O,Merdinoglu D,Guesdon JL

    更新日期:1989-06-01 00:00:00

  • miR-410-5p promotes the development of diabetic cardiomyopathy by suppressing PIM1-induced anti-apoptosis.

    abstract::Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myoc...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Xia X,Liang Y,Zheng W,Lin D,Sun S

    更新日期:2020-08-01 00:00:00

  • Human papillomavirus type 33 DNA and E6-E7 transcripts in late passages of the UT-DEC-1 vaginal keratinocyte cell line.

    abstract::Transcription of human papillomavirus (HPV) type 33 early region was analysed in the UT-DEC-1 keratinocyte cell line, which has been derived from a HPV-33-containing mild vaginal dysplasia. Fifteen cDNA clones from transcripts from the E6-E7 open reading frames were constructed and analysed. Most clones represented vi...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Auvinen E,Hietanen S,Gissmann L,Alonso A

    更新日期:1998-02-01 00:00:00

  • Identification of six novel CFTR mutations in a sample of Italian cystic fibrosis patients.

    abstract::The spectrum of cystic fibrosis (CF) mutations has been determined in many populations of different ethnic and geographic origins. However, in the south of Europe, the commonest mutation, delta F508, accounts for only about 50% of CF chromosomes, while identification of most of the other mutant alleles has not been ac...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Férec C,Novelli G,Verlingue C,Quéré I,Dallapiccola B,Audrézet MP,Mercier B

    更新日期:1995-04-01 00:00:00

  • Diagnosis of mouse hepatitis virus contamination in mouse population by using nude mice and RT-PCR.

    abstract::Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficult. Reverse transcription plus polymerase chain reaction (RT-PCR) was ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wang RF,Campbell WL,Cao WW,Colvert RM,Holland MA,Cerniglia CE

    更新日期:1999-02-01 00:00:00

  • Serogroup specific single and multiplex PCR with pre-enrichment culture and immuno-magnetic bead capture for identifying strains of D. nodosus in sheep with footrot prior to vaccination.

    abstract::The identification of Dichelobacter nodosus present in a flock is a prerequisite to specific (autogenous) vaccination. Current methods of identification of the serogroup present in a population requires that the organisms be isolated, identified visually in mixed culture on streak plates, subcultured to purify and sub...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Dhungyel OP,Whittington RJ,Egerton JR

    更新日期:2002-08-01 00:00:00

  • A multiplex RT-PCR for detection of type A influenza virus and differentiation of avian H5, H7, and H9 hemagglutinin subtypes.

    abstract::A multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) was developed and optimized for the detection of type A influenza virus; the assay simultaneously differentiates avian H5, H7 and H9 hemagglutinin subtypes. Four sets of specific oligonucleotide primers were used in this test for type A influenza vi...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Xie Z,Pang YS,Liu J,Deng X,Tang X,Sun J,Khan MI

    更新日期:2006-06-01 00:00:00

  • Use of RAPD for the detection of genetic variation in the human blood fluke, Schistosoma japonicum, from mainland China.

    abstract::A random amplified polymorphic DNA (RAPD) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of Schistosoma japonicum from seven geographical locations (Sj1: Zhejiang; Sj2: Anhui; Sj3: Jiangxi; Sj4: Hunan; Sj5: Hubei; Sj6: Sichuan; Sj7: Yunnan) of the People's Republic of China. D...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Gasser RB,Bao-Zhen Q,Nansen P,Johansen MV,Bøgh H

    更新日期:1996-10-01 00:00:00

  • Polymorphisms in the Human SNAIL (SNAI1) gene.

    abstract::The human SNAIL is an important developmental protein involved in the formation of mesoderm and neural crest. The protein contains three classic and one atypical zinc-finger motif. The SNAI1 gene is composed of three exons. We have identified three SNPs in non-coding regions, two in the 5'UTR and one in intron 1, whic...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Okajima K,Paznekas WA,Burstyn T,Jabs EW

    更新日期:2001-02-01 00:00:00

  • Human metapneumovirus uses endocytosis pathway for host cell entry.

    abstract::Human metapneumovirus (hMPV) is a prevalent pathogen worldwide and causes various respiratory infections. Although it is a critical pathogen in pediatric patients, it is unclear how it enters host cells. In this study, we focused on hMPV cell entry using two kinds of cell lines (Vero E6 and LLC-MK2), which are most co...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yang H,He H,Tan B,Liu E,Zhao X,Zhao Y

    更新日期:2016-08-01 00:00:00

  • SNP genotyping with FRET probes. Optimizing the resolution of heterozygotes.

    abstract::Analysis of single nucleotide polymorphisms by PCR with fluorescence resonance energy transfer (FRET) probes often can produce a result where the melting peak corresponding to perfectly matched sequence (A allele) has a smaller area than the peak corresponding to the allele with a mismatch (B allele). This imbalance c...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Martínez-García A,Sastre I,Tenorio R,Bullido MJ

    更新日期:2004-08-01 00:00:00

  • Rapid diagnosis of spinal muscular atrophy using tetra-primer ARMS PCR assay: simultaneous detection of SMN1 and SMN2 deletion.

    abstract::Spinal muscular atrophy (SMA), the leading genetic cause of death in childhood, is an autosomal recessive neuromuscular disorder characterized by progressive muscle weakness, associated with deletions of the survival motor neuron 1 (SMN1) gene. Approximately 94% of SMA patients carry homologous deletions of SMN1 exon(...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Baris I,Etlik O,Koksal V,Arican-Baris ST

    更新日期:2010-06-01 00:00:00

  • Rapid PCR using nested primers of the 16S rRNA and the hippuricase (hip O) genes to detect Campylobacter jejuni and Campylobacter coli in environmental samples.

    abstract::Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bang DD,Wedderkopp A,Pedersen K,Madsen M

    更新日期:2002-10-01 00:00:00

  • High-throughput qualitative multiplex 5' nuclease assay using post-only PCR analysis.

    abstract::Homogenous fluorescence PCR assays offer distinct advantages for qualitative testing and are gaining immense popularity in fields like diagnostic microbiology. To meet the demand of high-volume laboratories, we developed a protocol for qualitative multiplex 5' nuclease assays using post-only PCR analysis. This novel a...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Antonishyn NA,McDonald RR,Chan EL,Horsman GB

    更新日期:2005-10-01 00:00:00

  • Use of a nested PCR method for the detection of astrovirus serotype 1 in human faecal material.

    abstract::In this paper we describe a reverse-transcription nested polymerase chain reaction method for detecting human astrovirus serotype 1. It has been evaluated on 56 UK diarrhoeal stool specimens and six non-UK specimens. The method has greater sensitivity than electron microscopy and may be a useful test in areas such as ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Shi M,Sikotra S,Lee T,Kurtz JB,Getty B,Hart CA,Myint SH

    更新日期:1994-12-01 00:00:00

  • Differentiation of three species of ixodid tick, Dermacentor andersoni, D. variabilis and D. albipictus, by PCR-based approaches using markers in ribosomal DNA.

    abstract::Three species of Dermacentor, Dermacentor albipictus, Dermacentor andersoni and Dermacentor variabilis, commonly occur in Canada. D. andersoni and D. variabilis are morphologically similar and are important vectors of human and animal pathogens. A practical polymerase chain reaction (PCR) assay, based on the amplifica...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Dergousoff SJ,Chilton NB

    更新日期:2007-10-01 00:00:00

  • Detection of shigellae, enteroinvasive and enterotoxigenic Escherichia coli using the polymerase chain reaction (PCR) in patients returning from tropical countries.

    abstract::We have used the polymerase chain reaction (PCR) to detect shigellae, EIEC and ETEC in stool specimens of diarrhoeic patients returning from tropical countries. As compared to culture (7.1% positive specimens), which recognizes only Shigella strains, PCR performed on bacterial growth from directly inoculated MacConkey...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lüscher D,Altwegg M

    更新日期:1994-08-01 00:00:00

  • Use of antibodies against the P36 protein of Mycoplasma hyopneumoniae for the identification of M. hyopneumoniae strains.

    abstract::Mycoplasma hyopneumoniae, the principal aetiological agent of porcine enzootic pneumonia, synthesizes a 36 kDa protein (P36) which is an early and strong immunogenic factor in experimentally and naturally infected swine. Polyclonal antibodies were made against the recombinant P36 protein in rabbits and used for the id...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Stipkovits L,Nicolet J,Haldimann A,Frey J

    更新日期:1991-12-01 00:00:00

  • Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism.

    abstract::We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run usin...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Gromadzka B,Smietanka K,Dragun J,Minta Z,Gora-Sochacka A,Szewczyk B

    更新日期:2008-10-01 00:00:00

  • Rapid and visual detection of dengue virus using recombinase polymerase amplification method combined with lateral flow dipstick.

    abstract::Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

    更新日期:2019-08-01 00:00:00

  • Microduplication of 10q26.3 in a Chinese hypertriglyceridemia patient.

    abstract::Hypertriglyceridemia (HTG) plays an important role in the development and progression of atherosclerosis. It is inherited in an autosomal dominant pattern with a frequency of approximately 1:1,000,000 worldwide. Previous study has demonstrated that more than six genes underlie this disorder. In addition, copy number v...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Li JJ,Chen YQ,Fan LL,Jin JY,Guo S,Xiang R

    更新日期:2018-02-01 00:00:00

  • Real-time PCR using SYBR Green for the detection of Shigella spp. in food and stool samples.

    abstract::Shigella spp are exquisitely fastidious Gram negative organisms that frequently get missed in the detection by traditional culture methods. For this reason, this work has adapted a classical PCR for detection of Shigella in food and stool specimens to real-time PCR using the SYBR Green format. This method follows a me...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Mokhtari W,Nsaibia S,Gharbi A,Aouni M

    更新日期:2013-02-01 00:00:00

  • A new method for species identification and differentiation of Mycobacterium chelonae complex based on amplified hsp65 restriction analysis (AHSPRA).

    abstract::Members of the Mycobacterium chelonae complex (MCC), namely M. chelonae, Mycobacterium abscessus and Mycobacterium immunogenum, have been implicated in nosocomial infections and occupational respiratory illnesses like hypersensitivity pneumonitis (HP) associated with contaminated metalworking fluid (MWF) exposures. Cl...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Selvaraju SB,Khan IU,Yadav JS

    更新日期:2005-04-01 00:00:00

  • Specific detection of common pathogens of acute bacterial meningitis using an internally controlled tetraplex-PCR assay.

    abstract::Accurate and timely diagnosis of acute bacterial meningitis is critical for antimicrobial treatment of patients. Although PCR-based methods have been widely used for the diagnosis of acute meningitis caused by bacterial pathogens, the main disadvantage of these methods is their high cost. This disadvantage has hampere...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Farahani H,Ghaznavi-Rad E,Mondanizadeh M,MirabSamiee S,Khansarinejad B

    更新日期:2016-08-01 00:00:00

  • Evaluation of thymidylate synthase RNA expression by polymerase chain reaction.

    abstract::5-Fluorouracil (5FU), an antimetabolite often used for the treatment of breast cancer, binds to and inactivates the enzyme thymidylate synthase (TS). Measurement of TS levels may be useful in examining resistance to 5FU, but current methods involving ligand binding assays present considerable problems due to the low b...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Elledge RM,Okuyama N,Fitzgerald SD,Fugua SA

    更新日期:1994-02-01 00:00:00

  • Modification of the DNA colony hybridization technique for multiple filter analysis.

    abstract::Inexpensive fiberglass mesh window screens were used as spacers between colony blot filters to increase the number of bacterial isolates that could be tested by DNA colony hybridization. Sixty filters with up to 48 isolates per filter were tested at one time. This modified technique reduced the amount of radiolabelled...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Kaysner CA,Weagant SD,Hill WE

    更新日期:1988-12-01 00:00:00

  • Taxonomic and phylogenetic status of non-tuberculous mycobacteria in a Caribbean setting.

    abstract::This report describes detailed taxonomic and phylogenetic analysis of 15 non-tuberculous mycobacteria (NTMs) isolated from human pathological specimens in a Caribbean setting (12 slow-growers and three rapid-growers) that were not identified by cultural and biochemical tests and drug-susceptibility results. These isol...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ferdinand S,Legrand E,Goh KS,Berchel M,Mazzarelli G,Sola C,Tortoli E,Rastogi N

    更新日期:2004-12-01 00:00:00

  • Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

    abstract::Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Axton RA,Brock DJ

    更新日期:1994-06-01 00:00:00

  • Construction of an internal control for the detection of Chlamydia pneumoniae by PCR.

    abstract::For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ursi D,Ieven M,Van Bever HP,Goossens H

    更新日期:1998-08-01 00:00:00