Enhanced discrimination of single nucleotide polymorphisms using 3' nucleotide differences in ligase detection reaction probes.

abstract：:Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficult. Reverse transcription plus polymerase chain reaction (RT-PCR) was ...

journal_title：Molecular and cellular probes

authors： Wang RF,Campbell WL,Cao WW,Colvert RM,Holland MA,Cerniglia CE

更新日期：1999-02-01 00:00:00

abstract：:The progressive myoclonus epilepsy of Lafora type (LD) is an autosomal recessive disorder caused by mutations in the EPM2A gene. We demonstrated recently that EPM2A encodes a dual-specificity phosphatase that is primarily associated with polyribosomes. In the present study, we screened for mutations in the EPM2A gene ...

journal_title：Molecular and cellular probes

authors： Ganesh S,Shoda K,Amano K,Uchiyama A,Kumada S,Moriyama N,Hirose S,Yamakawa K

更新日期：2001-10-01 00:00:00

abstract：:The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

journal_title：Molecular and cellular probes

authors： Chaubert P,Burri N,Cousin P,Shaw P

更新日期：2001-06-01 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) to detect shigellae, EIEC and ETEC in stool specimens of diarrhoeic patients returning from tropical countries. As compared to culture (7.1% positive specimens), which recognizes only Shigella strains, PCR performed on bacterial growth from directly inoculated MacConkey...

journal_title：Molecular and cellular probes

authors： Lüscher D,Altwegg M

更新日期：1994-08-01 00:00:00

abstract：:Variable number tandem repeat (VNTR) is a frequently employed typing method of Mycobacterium avium paratuberculosis (MAP) isolates. Based on whole genome sequencing in a previous study, allelic diversity at some VNTR loci seems to over- or under-estimate the actual phylogenetic variance among isolates. Interestingly, ...

journal_title：Molecular and cellular probes

authors： Fawzy A,Zschöck M,Ewers C,Eisenberg T

更新日期：2016-06-01 00:00:00

abstract：:The unique ability of homopyrimidine peptide nucleic acid (PNA) to strand invade homopurine sites of duplex DNA offers a potential alternative to existing techniques for rapid detection of PCR products. From gel shift studies, PNA was found to specifically strand invade homopurine sites that had been incorporated into...

journal_title：Molecular and cellular probes

authors： Drewe LJ,Brightwell G,Hall EA

更新日期：2000-10-01 00:00:00

abstract：:At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed...

journal_title：Molecular and cellular probes

authors： Newton LA,Chilton NB,Monti JR,Bjørn H,Várady M,Christensen CM,Gasser RB

更新日期：1997-04-01 00:00:00

abstract：:The molecular tag vaccine against Brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. They can discriminate vaccinated animals and humans from those naturally infected. In this study, we constructed a gene deletion mutant strain, B. abortus S19 virB5 with a molecu...

journal_title：Molecular and cellular probes

authors： Tan W,Wang XR,Nie Y,Wang C,Cheng LQ,Wang XC,Zhang R,Yan GM

更新日期：2012-06-01 00:00:00

abstract：:Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. We developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine. Consider...

journal_title：Molecular and cellular probes

authors： Mitui M,Leos NK,Lacey D,Doern C,Rogers BB,Park JY

更新日期：2013-10-01 00:00:00

abstract：INTRODUCTION:Treatment in metastatic colorectal cancer (mCRC) has expanded with monoclonal antibodies targeting epidermal growth factor receptor, but is restricted to patients with a wild-type (WT) KRAS mutational status. The most sensitive assays for KRAS mutation detection in formalin-fixed paraffin embedded (FFPE) t...

journal_title：Molecular and cellular probes

authors： Pérez-Báez W,García-Latorre EA,Maldonado-Martínez HA,Coronado-Martínez I,Flores-García L,Taja-Chayeb L

更新日期：2017-10-01 00:00:00

abstract：:The spectrum of cystic fibrosis (CF) mutations has been determined in many populations of different ethnic and geographic origins. However, in the south of Europe, the commonest mutation, delta F508, accounts for only about 50% of CF chromosomes, while identification of most of the other mutant alleles has not been ac...

journal_title：Molecular and cellular probes

authors： Férec C,Novelli G,Verlingue C,Quéré I,Dallapiccola B,Audrézet MP,Mercier B

更新日期：1995-04-01 00:00:00

abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-rea...

journal_title：Molecular and cellular probes

authors： Chen HT,Zhang J,Ma YP,Ma LN,Ding YZ,Liu XT,Cai XP,Ma LQ,Zhang YG,Liu YS

更新日期：2010-04-01 00:00:00

abstract：:We have examined the relationship between Chlamydia trachomatis found in clinical samples in which the cryptic plasmid was absent and known serovars of C. trachomatis. PCR and RNase protection assays were used to compare 12 C. trachomatis serovars and a plasmidless L2 serovar strain with the reactivity of clinical spe...

journal_title：Molecular and cellular probes

authors： An Q,Olive DM

更新日期：1994-10-01 00:00:00

abstract：:The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs i...

journal_title：Molecular and cellular probes

authors： Nagaraju J,Kathirvel M,Subbaiah EV,Muthulakshmi M,Kumar LD

更新日期：2002-02-01 00:00:00

abstract：:A multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) was developed and optimized for the detection of type A influenza virus; the assay simultaneously differentiates avian H5, H7 and H9 hemagglutinin subtypes. Four sets of specific oligonucleotide primers were used in this test for type A influenza vi...

journal_title：Molecular and cellular probes

authors： Xie Z,Pang YS,Liu J,Deng X,Tang X,Sun J,Khan MI

更新日期：2006-06-01 00:00:00

abstract：:We have developed a new methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase (TdT) using a thioflavin T probe. This method is highly selective and sensitive. The fluorescence intensity was direct proportion to Dam MTase concentration in...

journal_title：Molecular and cellular probes

authors： Ma C,Liu H,Li W,Chen H,Jin S,Wang J,Wang J

更新日期：2016-04-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a prevalent pathogen worldwide and causes various respiratory infections. Although it is a critical pathogen in pediatric patients, it is unclear how it enters host cells. In this study, we focused on hMPV cell entry using two kinds of cell lines (Vero E6 and LLC-MK2), which are most co...

journal_title：Molecular and cellular probes

authors： Yang H,He H,Tan B,Liu E,Zhao X,Zhao Y

更新日期：2016-08-01 00:00:00

abstract：:Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a target DNA sequence. We performed SDA in the presence of a 5'-32P-oligodeoxynucleotide detector probe that contains a target binding sequence at its 3'-end and a recognition site for the restriction enzyme HincII at its 5'-end wh...

journal_title：Molecular and cellular probes

authors： Walker GT,Nadeau JG,Linn CP

更新日期：1995-12-01 00:00:00

abstract：:Arcobacter is a recently described species, previously considered part of the Campylobacter family. A sensitive assay such as that provided by PCR could help to distinguish the closely related Arcobacter from Campylobacter. A PCR method to specifically detect both Campylobacter jejuni and Arcobacter butzleri in the sa...

journal_title：Molecular and cellular probes

authors： Winters DK,Slavik MF

更新日期：2000-04-01 00:00:00

abstract：:Homogenous fluorescence PCR assays offer distinct advantages for qualitative testing and are gaining immense popularity in fields like diagnostic microbiology. To meet the demand of high-volume laboratories, we developed a protocol for qualitative multiplex 5' nuclease assays using post-only PCR analysis. This novel a...

journal_title：Molecular and cellular probes

authors： Antonishyn NA,McDonald RR,Chan EL,Horsman GB

更新日期：2005-10-01 00:00:00

abstract：:Non-pathogenic Burkholderia thailandensis may be used as a model for Burkholderia pseudomallei due to the genetic similarity of these species. Moreover, the experimental manipulation of B. thailandensis is safer. In this study, we constructed recombinant flagellin protein fragments of B. thailandensis E264 (FLAG300, F...

journal_title：Molecular and cellular probes

authors： Wajanarogana S,Nimnuch P,Thongmee A,Kritsiriwuthinan K

更新日期：2013-04-01 00:00:00

abstract：:To produce hybrids, one member of the parental line is genetically made male-sterile. This male-sterile trait is encoded by mitochondria so that it is maternally inherited. Consequently, the progeny of a male-sterile plant is fully sterile. Nevertheless, during the handling of cytoplasmic male-sterile seed stocks, som...

journal_title：Molecular and cellular probes

authors： Santoni S,Faivre-Rampant P,Moreau E,Bervillé A

更新日期：1991-02-01 00:00:00

abstract：:The polymerase spiral reaction (PSR), a novel isothermal method for targeted DNA amplification, was effectively applied to detect Salmonella in artificially spiked pork. The specificity of the developed PSR was tested using 16 Salmonella and 15 non-Salmonella strains. The PSR assay was 10-fold more sensitive than conv...

journal_title：Molecular and cellular probes

authors： Momin KM,Milton AAP,Ghatak S,Thomas SC,Priya GB,Das S,Shakuntala I,Sanjukta R,Puro KU,Sen A

更新日期：2020-04-01 00:00:00

abstract：:TTR amyloidosis (ATTR) is a fatal condition caused by extracellular deposits of misfolded transthyretin. Patients often present with cardiac disease, but manifestations may also involve other organs including the peripheral nervous system. ATTR is considered familial when heterozygous mutations in the TTR gene are pre...

journal_title：Molecular and cellular probes

authors： Jahic A,Bock A,Duca F,Bonderman D,Mascherbauer J,Windhager R,Auer-Grumbach M,Beetz C

更新日期：2018-10-01 00:00:00

abstract：:New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env...

journal_title：Molecular and cellular probes

authors： Sabunciyan S,Mandelberg N,Rabkin CS,Yolken R,Viscidi R

更新日期：2011-04-01 00:00:00

abstract：:A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larva...

journal_title：Molecular and cellular probes

authors： Campbell BE,Rabelo EM,Hofmann A,Hu M,Gasser RB

更新日期：2010-08-01 00:00:00

abstract：:Microsatellites have recently been used for linkage analysis of genetic diseases and for DNA fingerprinting in forensic medicine. In the present study the heterozygosity, PIC values and allele distributions of four microsatellites, D8S85, D8S88, D5S346 and D7S460, in an Italian population have been investigated. After...

journal_title：Molecular and cellular probes

authors： Sirchia SM,Garagiola I,De Andreis C,Gazzoli I,Gramegna M,Colucci G

更新日期：1996-04-01 00:00:00

abstract：:Mutations in the WDR45 gene have been identified as causative for the only X-linked type of neurodegeneration with brain iron accumulation (NBIA), clinically characterized by global developmental delay in childhood, followed by a secondary neurological decline with parkinsonism and/or dementia in adolescence or early ...

journal_title：Molecular and cellular probes

authors： Hoffjan S,Ibisler A,Tschentscher A,Dekomien G,Bidinost C,Rosa AL

更新日期：2016-02-01 00:00:00

abstract：:Pre-mRNA processing factor 4 (PRPF4), a core protein in U4/U6 snRNP, maintains snRNP structures by interacting with PRPF3 and cyclophilin H. Expression of the PRPF4 gene affects cell survival as well as apoptosis and is responsible for retinitis pigmentosa (RP). Proteomics analysis shows that PRPF4 may be a therapeuti...

journal_title：Molecular and cellular probes

authors： Park S,Han SH,Kim HG,Jeong J,Choi M,Kim HY,Kim MG,Park JK,Han JE,Cho GJ,Kim MO,Ryoo ZY,Choi SK

更新日期：2019-10-01 00:00:00

abstract：:A nucleic acid hybridization assay for the detection of the pilin gene of Neisseria gonorrhoeae has been devised. The method involves solution hybridization of pilin specific synthetic oligonucleotide probes to genomic DNA in crude cell lysates. This is followed by capture of the probe-target complex onto a microtitre...

journal_title：Molecular and cellular probes

authors： Kolberg JA,Besemer DJ,Stempien MM,Urdea MS

更新日期：1989-03-01 00:00:00