Multiplex PCR detection of Campylobacter jejuni and Arcobacter butzleri in food products.

abstract：:Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a...

journal_title：Molecular and cellular probes

authors： French DJ,Archard CL,Brown T,McDowell DG

更新日期：2001-12-01 00:00:00

abstract：:Idiopathic generalized epilepsy (IGE) comprises a heterogeneous group of disorders, in which a high genetic predisposition and a complex mode of inheritance have been suggested. However, genes, which confer liability to common IGE subtypes including juvenile myoclonic epilepsy (JME) and childhood absence epilepsy (CAE...

journal_title：Molecular and cellular probes

authors： Haug K,Kremerskothen J,Hallmann K,Sander T,Dullinger J,Rau B,Beyenburg S,Lentze MJ,Barnekow A,Elger CE,Propping P,Heils A

更新日期：2000-08-01 00:00:00

abstract：:The unique ability of homopyrimidine peptide nucleic acid (PNA) to strand invade homopurine sites of duplex DNA offers a potential alternative to existing techniques for rapid detection of PCR products. From gel shift studies, PNA was found to specifically strand invade homopurine sites that had been incorporated into...

journal_title：Molecular and cellular probes

authors： Drewe LJ,Brightwell G,Hall EA

更新日期：2000-10-01 00:00:00

abstract：:Non-small-cell lung carcinoma (NSCLC) accounts for approximately 80% of lung cancers with a high metastatic potential. Elucidating the mechanism of NSCLC metastasis will provide new promising targets for NSCLC therapy and benefit its prognosis. Plasmacytoma variant translocation 1 (PVT1) has been proven to be overexpr...

journal_title：Molecular and cellular probes

authors： Su XH,Zhu YR,Hou YJ,Li K,Dong NH

更新日期：2020-12-01 00:00:00

abstract：:Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

journal_title：Molecular and cellular probes

authors： Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

更新日期：2019-08-01 00:00:00

abstract：:A simple assay format was developed for the direct detection of C. trachomatis rRNA utilizing ligation of recombinant MDV-1 probe RNA fragments hybridized to 23S rRNA after capture and release from a solid support. Assay background (equivalent to 10(4) targets) was suppressed by blocking sequences in the 5' MDV report...

journal_title：Molecular and cellular probes

authors： Stefano JE,Genovese L,An Q,Lu L,McCarty J,Du Y,Stefano K,Burg JL,King W,Lane DJ

更新日期：1997-12-01 00:00:00

abstract：:The human SNAIL is an important developmental protein involved in the formation of mesoderm and neural crest. The protein contains three classic and one atypical zinc-finger motif. The SNAI1 gene is composed of three exons. We have identified three SNPs in non-coding regions, two in the 5'UTR and one in intron 1, whic...

journal_title：Molecular and cellular probes

authors： Okajima K,Paznekas WA,Burstyn T,Jabs EW

更新日期：2001-02-01 00:00:00

abstract：BACKGROUND:Androgen receptor (AR) and long non-coding RNAs (lncRNA) play important roles in the initiation and progression of prostate cancer (PCa). The present study was designed to investigate whether lncRNA growth arrest-specific 5 (GAS5) is involved in the regulation of dexamethasone on the proliferation of AR+ PCa...

journal_title：Molecular and cellular probes

authors： Hu J,Deng J,Cao R,Xiong S,Guo J

更新日期：2020-10-01 00:00:00

abstract：:To find a specific method for HLA-B27 typing, we tested an HLA-B27-specific polymerase chain reaction. This method was used for screening 100 randomly selected blood donors, 10 of them being HLA-B27 positive. A flow cytometric method and this PCR method were compared. ...

journal_title：Molecular and cellular probes

authors： Lucotte G,Burckel A

更新日期：1997-08-01 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) to detect shigellae, EIEC and ETEC in stool specimens of diarrhoeic patients returning from tropical countries. As compared to culture (7.1% positive specimens), which recognizes only Shigella strains, PCR performed on bacterial growth from directly inoculated MacConkey...

journal_title：Molecular and cellular probes

authors： Lüscher D,Altwegg M

更新日期：1994-08-01 00:00:00

abstract：:An alkaline phosphatase (AP)-labeled genus-specific oligonucleotide probe was developed to detect and enumerate vibrios in shrimp larvae and their surrounding environment. The probe was evaluated using 35 laboratory isolates of Vibrio species and 29 isolates of non-vibrio species. The probe was specific for the Vibrio...

journal_title：Molecular and cellular probes

authors： Raghunath P,Karunasagar I,Karunasagar I

更新日期：2007-08-01 00:00:00

abstract：:The ability to preserve and transport human cells in a stable medium over long distances is critical to collaborative efforts and the advancement of knowledge in the study of human disease. This is particularly important in the study of rare diseases. Recently, advancements in the understanding of renal ciliopathies h...

journal_title：Molecular and cellular probes

authors： Dewhurst RM,Molinari E,Sayer JA

更新日期：2021-01-08 00:00:00

abstract：:Spinal muscular atrophy (SMA), the leading genetic cause of death in childhood, is an autosomal recessive neuromuscular disorder characterized by progressive muscle weakness, associated with deletions of the survival motor neuron 1 (SMN1) gene. Approximately 94% of SMA patients carry homologous deletions of SMN1 exon(...

journal_title：Molecular and cellular probes

authors： Baris I,Etlik O,Koksal V,Arican-Baris ST

更新日期：2010-06-01 00:00:00

abstract：:Tomato black ring virus (TBRV) infects a wide range of economically important plant species worldwide. In the present study we developed a locked nucleic acid (LNA) real-time RT-PCR assay for accurate detection of genetically diverse TBRV isolates collected from different hosts. The assay based on the LNA probe has a ...

journal_title：Molecular and cellular probes

authors： Hasiów-Jaroszewska B,Rymelska N,Borodynko N

更新日期：2015-02-01 00:00:00

abstract：:Wild-type p53 (wtp53) is a tumour suppressor gene involved in cell cycle regulation. The mdm2 protein can complex with the p53 protein and influence its function as a regulator of cell growth. To detect and quantify wtp53 and mdm2 mRNA expression, we established the competitive reverse transcription/polymerase chain r...

journal_title：Molecular and cellular probes

authors： Totzke G,Sachinidis A,Vetter H,Ko Y

更新日期：1996-12-01 00:00:00

abstract：:Hypertriglyceridemia (HTG) plays an important role in the development and progression of atherosclerosis. It is inherited in an autosomal dominant pattern with a frequency of approximately 1:1,000,000 worldwide. Previous study has demonstrated that more than six genes underlie this disorder. In addition, copy number v...

journal_title：Molecular and cellular probes

authors： Li JJ,Chen YQ,Fan LL,Jin JY,Guo S,Xiang R

更新日期：2018-02-01 00:00:00

abstract：:PCR detection and quantification of vaginal lactobacilli remains problematic because of the high level of genetic heterogeneity and taxonomic complexity within the genus Lactobacillus. The aim of the present study was to identify conserved sequences among the genomes of major species of vaginal lactobacilli that could...

journal_title：Molecular and cellular probes

authors： Demkin VV,Koshechkin SI,Slesarev A

更新日期：2017-04-01 00:00:00

abstract：:Multiplex ligation dependent probe amplification (MLPA) assays were designed for the genes HEXB (OMIM: 606873), GM2A (OMIM: 613109) and SMARCAL1 (OMIM: 606622) of humans. Two sets of synthetic MLPA probes for these coding exons were tested. Changes in copy numbers were detected as well as single nucleotide polymorphis...

journal_title：Molecular and cellular probes

authors： Sobek AK,Evers C,Dekomien G

更新日期：2013-02-01 00:00:00

abstract：PURPOSE:This study aimed to analyze the relationships of long non-coding RNAs (lncRNAs) and protein-coding genes in lung squamous cell carcinoma (LUSC). METHODS:RNA-seq data of LUSC deposited in the TCGA database were used to identify differentially expressed protein-coding genes (DECGs) and differentially expressed l...

journal_title：Molecular and cellular probes

authors： Wu X,Ruan L,Yang Y,Mei Q

更新日期：2016-06-01 00:00:00

abstract：:Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific prim...

journal_title：Molecular and cellular probes

authors： Wan C,Cheng L,Chen C,Liu R,Shi S,Fu G,Chen H,Fu Q,Huang Y

更新日期：2019-10-01 00:00:00

abstract：:Genetic polymorphism among patients with acute lymphoblastic leukemia (ALL) is an important factor in the effectiveness and toxicity of anti-leukemic drugs. Genotyping of various polymorphisms that impact the outcome of anti-leukemic drug therapy (pharmacogenetics) presents an attractive approach for developing indivi...

journal_title：Molecular and cellular probes

authors： Kang HJ,Oh Y,Chun SM,Seo YJ,Shin HY,Kim CW,Ahn HS,Han BD

更新日期：2008-06-01 00:00:00

abstract：:Analysis of single nucleotide polymorphisms by PCR with fluorescence resonance energy transfer (FRET) probes often can produce a result where the melting peak corresponding to perfectly matched sequence (A allele) has a smaller area than the peak corresponding to the allele with a mismatch (B allele). This imbalance c...

journal_title：Molecular and cellular probes

authors： Martínez-García A,Sastre I,Tenorio R,Bullido MJ

更新日期：2004-08-01 00:00:00

abstract：:We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing ...

journal_title：Molecular and cellular probes

authors： Zhu YC,Mitchell KK,Nazarian EJ,Escuyer VE,Musser KA

更新日期：2015-12-01 00:00:00

abstract：:In this paper we describe a reverse-transcription nested polymerase chain reaction method for detecting human astrovirus serotype 1. It has been evaluated on 56 UK diarrhoeal stool specimens and six non-UK specimens. The method has greater sensitivity than electron microscopy and may be a useful test in areas such as ...

journal_title：Molecular and cellular probes

authors： Shi M,Sikotra S,Lee T,Kurtz JB,Getty B,Hart CA,Myint SH

更新日期：1994-12-01 00:00:00

abstract：:The ligase detection reaction (LDR) is a highly specific genotyping method for single nucleotide variations. Although LDR typically discriminates single nucleotide polymorphism (SNP) alleles at the 3' end of so-called LDR discriminating probes, we designed probes in which the position of nucleotide differences for dis...

journal_title：Molecular and cellular probes

authors： Asari M,Omura T,Maseda C,Shiono H,Tasaki Y,Matsubara K,Shimizu K

更新日期：2010-12-01 00:00:00

abstract：:We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant d...

journal_title：Molecular and cellular probes

authors： Hong SH,Song J,Kim JQ

更新日期：2001-04-01 00:00:00

abstract：:For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

journal_title：Molecular and cellular probes

authors： Ursi D,Ieven M,Van Bever HP,Goossens H

更新日期：1998-08-01 00:00:00

abstract：:Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 196...

journal_title：Molecular and cellular probes

authors： Komissarov A,Fadeev A,Kosheleva A,Sintsova K,Grudinin M

更新日期：2017-10-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) is responsible for severe infections in immunocompromised patients. Viral load has recently been identified as one of the major risk factors for subsequent development of HCMV disease. In this context, we developed a protocol allowing rapid, sensitive and precise quantification of HCMV DNA...

journal_title：Molecular and cellular probes

authors： Poirier-Toulemonde AS,Imbert-Marcille BM,Ferré-Aubineau V,Besse B,Le Roux MG,Cantarovich D,Billaudel S

更新日期：1997-02-01 00:00:00

abstract：:Citrus plants are natural hosts of five viroid species and a large number of sequence variants. Because of their small size, viroids lend themselves to various RT-PCR approaches for their detection and further characterization. The one-step RT-PCR approach proposed here is based on the synthesis of viroid-cDNA by reve...

journal_title：Molecular and cellular probes

authors： Bernad L,Duran-Vila N

更新日期：2006-04-01 00:00:00