Rapid and sensitive detection of Chlamydia trachomatis using a ligatable binary RNA probe and Q beta replicase.


:A simple assay format was developed for the direct detection of C. trachomatis rRNA utilizing ligation of recombinant MDV-1 probe RNA fragments hybridized to 23S rRNA after capture and release from a solid support. Assay background (equivalent to 10(4) targets) was suppressed by blocking sequences in the 5' MDV reporter probe fragment complementary to the 3' fragment by prehybridization of a DNA oligonucleotide. A pair of reporter fragments bearing a deletion within the region, obtained by a hydrid-selection-amplification protocol, yielded a low level of assay background which was reduced to < 2% with a blocker directed against the remaining pairing sequence. This probe set showed a sensitivity of 10(3) molecules of 23S rRNA (> 95% responding) and could detect a single elementary body (EB) of Chlamydia trachomatis or 1-10 EB added to a clinical matrix of pooled negative human cervical swab samples. The time of first appearance of amplification products by real-time fluorescence detection showed a linear response to log increases in the target level over a 10(5)-fold range, permitting the determination of target level within an order of magnitude. The assay showed approximately 10(9)-fold discrimination over Chlamydia pneumonae (TWAR) rRNA. High levels of cultured C. albicans, E. coli, S. aureus, or N. gonorrhoeae had no detectable effect on assay background or the ability to detect a single elementary body.


Mol Cell Probes


Stefano JE,Genovese L,An Q,Lu L,McCarty J,Du Y,Stefano K,Burg JL,King W,Lane DJ




Has Abstract


1997-12-01 00:00:00














  • Mutation screening of the chromosome 8q24.3-human activity-regulated cytoskeleton-associated gene (ARC) in idiopathic generalized epilepsy.

    abstract::Idiopathic generalized epilepsy (IGE) comprises a heterogeneous group of disorders, in which a high genetic predisposition and a complex mode of inheritance have been suggested. However, genes, which confer liability to common IGE subtypes including juvenile myoclonic epilepsy (JME) and childhood absence epilepsy (CAE...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Haug K,Kremerskothen J,Hallmann K,Sander T,Dullinger J,Rau B,Beyenburg S,Lentze MJ,Barnekow A,Elger CE,Propping P,Heils A

    更新日期:2000-08-01 00:00:00

  • Use of RAPD for the detection of genetic variation in the human blood fluke, Schistosoma japonicum, from mainland China.

    abstract::A random amplified polymorphic DNA (RAPD) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of Schistosoma japonicum from seven geographical locations (Sj1: Zhejiang; Sj2: Anhui; Sj3: Jiangxi; Sj4: Hunan; Sj5: Hubei; Sj6: Sichuan; Sj7: Yunnan) of the People's Republic of China. D...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Gasser RB,Bao-Zhen Q,Nansen P,Johansen MV,Bøgh H

    更新日期:1996-10-01 00:00:00

  • Pertactin-negative variants of Bordetella pertussis in New York State: a retrospective analysis, 2004-2013.

    abstract::The first report of pertactin-negative variants of Bordetella pertussis in the United States has raised questions about the role of acellular pertussis vaccines in the recent increase of pertussis cases. Our laboratory utilized a sequence-based method to identify mutations in the pertactin gene responsible for these v...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Quinlan T,Musser KA,Currenti SA,Zansky SM,Halse TA

    更新日期:2014-08-01 00:00:00

  • Genetic variation of the methylenetetrahydrofolate reductase and cystathionine beta-synthase genes in Korean patients with coronary artery disease and a new polymorphism in intron 7.

    abstract::We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant d...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hong SH,Song J,Kim JQ

    更新日期:2001-04-01 00:00:00

  • A new method for species identification and differentiation of Mycobacterium chelonae complex based on amplified hsp65 restriction analysis (AHSPRA).

    abstract::Members of the Mycobacterium chelonae complex (MCC), namely M. chelonae, Mycobacterium abscessus and Mycobacterium immunogenum, have been implicated in nosocomial infections and occupational respiratory illnesses like hypersensitivity pneumonitis (HP) associated with contaminated metalworking fluid (MWF) exposures. Cl...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Selvaraju SB,Khan IU,Yadav JS

    更新日期:2005-04-01 00:00:00

  • DNA typing of HLA-B27 by polymerase chain reaction.

    abstract::To find a specific method for HLA-B27 typing, we tested an HLA-B27-specific polymerase chain reaction. This method was used for screening 100 randomly selected blood donors, 10 of them being HLA-B27 positive. A flow cytometric method and this PCR method were compared. ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lucotte G,Burckel A

    更新日期:1997-08-01 00:00:00

  • PRPF4 is a novel therapeutic target for the treatment of breast cancer by influencing growth, migration, invasion, and apoptosis of breast cancer cells via p38 MAPK signaling pathway.

    abstract::Pre-mRNA processing factor 4 (PRPF4), a core protein in U4/U6 snRNP, maintains snRNP structures by interacting with PRPF3 and cyclophilin H. Expression of the PRPF4 gene affects cell survival as well as apoptosis and is responsible for retinitis pigmentosa (RP). Proteomics analysis shows that PRPF4 may be a therapeuti...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Park S,Han SH,Kim HG,Jeong J,Choi M,Kim HY,Kim MG,Park JK,Han JE,Cho GJ,Kim MO,Ryoo ZY,Choi SK

    更新日期:2019-10-01 00:00:00

  • Non-radioactive detection of Mycobacterium tuberculosis LCR products in a microtitre plate format.

    abstract::As part of the development of the ligase chain reaction (LCR) into a tool which can be used by a wide variety of researchers, we have investigated several analytical detection systems for the products of this amplification reaction. While early work with this technology has used gel electrophoresis to separate the LCR...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Winn-Deen ES,Batt CA,Wiedmann M

    更新日期:1993-06-01 00:00:00

  • Cell preservation methods and its application to studying rare disease.

    abstract::The ability to preserve and transport human cells in a stable medium over long distances is critical to collaborative efforts and the advancement of knowledge in the study of human disease. This is particularly important in the study of rare diseases. Recently, advancements in the understanding of renal ciliopathies h...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Dewhurst RM,Molinari E,Sayer JA

    更新日期:2021-01-08 00:00:00

  • Rapid PCR using nested primers of the 16S rRNA and the hippuricase (hip O) genes to detect Campylobacter jejuni and Campylobacter coli in environmental samples.

    abstract::Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bang DD,Wedderkopp A,Pedersen K,Madsen M

    更新日期:2002-10-01 00:00:00

  • Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism.

    abstract::We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run usin...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Gromadzka B,Smietanka K,Dragun J,Minta Z,Gora-Sochacka A,Szewczyk B

    更新日期:2008-10-01 00:00:00

  • Fluorescence-based DNA minisequence analysis for detection of known single-base changes in genomic DNA.

    abstract::We describe a rapid, automated method for direct detection of known single-base changes in genomic DNA. Fluorescence-based DNA minisequence analysis is employed in a template-dependent reaction which involves a single nucleotide extension of an oligonucleotide primer by the correct fluorescently-tagged dideoxynucleoti...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Kobayashi M,Rappaport E,Blasband A,Semeraro A,Sartore M,Surrey S,Fortina P

    更新日期:1995-06-01 00:00:00

  • Characterization of a Caenorhabditis elegans glc seven-like phosphatase (gsp) orthologue from Haemonchus contortus (Nematoda).

    abstract::A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larva...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Campbell BE,Rabelo EM,Hofmann A,Hu M,Gasser RB

    更新日期:2010-08-01 00:00:00

  • WDR45 mutations in Rett (-like) syndrome and developmental delay: Case report and an appraisal of the literature.

    abstract::Mutations in the WDR45 gene have been identified as causative for the only X-linked type of neurodegeneration with brain iron accumulation (NBIA), clinically characterized by global developmental delay in childhood, followed by a secondary neurological decline with parkinsonism and/or dementia in adolescence or early ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Hoffjan S,Ibisler A,Tschentscher A,Dekomien G,Bidinost C,Rosa AL

    更新日期:2016-02-01 00:00:00

  • What next-generation sequencing (NGS) technology has enabled us to learn about primary autosomal recessive microcephaly (MCPH).

    abstract::The impact that next-generation sequencing technology (NGS) is having on many aspects of molecular and cell biology, is becoming increasingly apparent. One of the most noticeable outcomes of the new technology in human genetics, has been the accelerated rate of identification of disease-causing genes. Especially for r...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Morris-Rosendahl DJ,Kaindl AM

    更新日期:2015-10-01 00:00:00

  • The construction and use of a PCR internal control.

    abstract::An example of the application and contruction of a polymerase chain reaction (PCR) internal control is presented. The internal control is synthesized in one PCR reaction. The primers used in this reaction possess 5' over-hanging ends which are identical to the primers used in the diagnostic reaction, whereas their 3' ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sachadyn P,Kur J

    更新日期:1998-10-01 00:00:00

  • Direct detection of vanA and vanB genes in clinical specimens for rapid identification of vancomycin resistant enterococci (VRE) using multiplex PCR.

    abstract::Surveillance for vancomycin resistant enterococci (VRE) by culture can be labour intensive and time consuming. We have developed a multiplex polymerase chain reaction (MPCR) which can be performed directly on the clinical specimen. The assay allows sensitive detection of enterococci with vanA - and vanB -mediated resi...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Petrich AK,Luinstra KE,Groves D,Chernesky MA,Mahony JB

    更新日期:1999-08-01 00:00:00

  • Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66.

    abstract::Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three reactions, through SYBR green I melting curve analysis. The method utilizes type-specific primer se...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Tsakogiannis D,Papacharalampous M,Toska E,Kyriakopoulou Z,Dimitriou TG,Ruether IG,Komiotis D,Markoulatos P

    更新日期:2015-02-01 00:00:00

  • DNA probe and polymerase chain reaction procedure for the specific detection of Serpulina hyodysenteriae.

    abstract::Serpulina (Treponema) hyodysenteriae, a Gram-negative anaerobic spirochete, is the causative agent of swine dysentery, a mucohaemorrhagic diarrheal disease in which lesions are confined to the large intestine of pigs. A DNA probe and polymerase chain reaction (PCR) amplification procedures which are specific, rapid , ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Harel J,Forget C

    更新日期:1995-04-01 00:00:00

  • Two novel single nucleotide polymorphisms in the promoter of the cellular retinoic acid binding protein II gene (CRABP-II).

    abstract::The cellular retinoic acid binding protein-II (CRABP-II) is an intracellular protein involved in the transmission of the vitamin A-derived signal which regulates genes responsible for lipid metabolism and adipocyte differentiation. Cellular Retinoic Acid Binding Protein-II gene (CRABP-II) (GDB 134819) is located on ch...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Salazar J,Ferré R,Vallvé JC,Pocoví M,Cabezas MC,Masana L,Ribalta J

    更新日期:2003-02-01 00:00:00

  • Comparison of multiplex PCR, PCR-ELISA and fluorogenic 5' nuclease PCR assays for detection of plasmid-bearing virulent Yersinia enterocolitica in swine feces.

    abstract::Swine are implicated as the principal animal reservoir for plasmid-bearing Yersinia enterocolitica (YEP(+)) strains that are pathogenic to humans. To evaluate the utility of the PCR for detection of YEP(+) strains in naturally-contaminated pig feces, samples were first enriched in Irgasan ticarcillin potassium chlorat...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bhaduri S

    更新日期:2002-06-01 00:00:00

  • Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.

    abstract::A simple dual-label hybridization test for normal and mutant cystic fibrosis (CF) alleles is described. The assay is based on time-resolved fluorometry (TRF), which allows the simultaneous detection of DNA probes labelled with different lanthanides from one hybridization reaction. DNA was liberated from dried blood di...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Iitiä A,Liukkonen L,Siitari H

    更新日期:1992-12-01 00:00:00

  • A simple procedure to differentiate ailA and ailNA gene variants among human pathogenic Yersinia enterocolitica strains.

    abstract::The identification and differentiation of the two variants of the ail gene, ailA from the more virulent American serotypes (08, 013a, 13b, 018, 020, 021) and ailNA from the less virulent non-American serotypes (03, 04, 05, 06, 09, 027 and 07, 8) was studied in a panel of 32 Yersinia enterocolitica human pathogenic iso...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sayada C,Picard B,Elion J,Krishnamoorthy R

    更新日期:1994-06-01 00:00:00

  • LncRNA GAS5 participates in the regulation of dexamethasone on androgen receptor -negative and -positive prostate cancer cell proliferation.

    abstract:BACKGROUND:Androgen receptor (AR) and long non-coding RNAs (lncRNA) play important roles in the initiation and progression of prostate cancer (PCa). The present study was designed to investigate whether lncRNA growth arrest-specific 5 (GAS5) is involved in the regulation of dexamethasone on the proliferation of AR+ PCa...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hu J,Deng J,Cao R,Xiong S,Guo J

    更新日期:2020-10-01 00:00:00

  • Microduplication of 10q26.3 in a Chinese hypertriglyceridemia patient.

    abstract::Hypertriglyceridemia (HTG) plays an important role in the development and progression of atherosclerosis. It is inherited in an autosomal dominant pattern with a frequency of approximately 1:1,000,000 worldwide. Previous study has demonstrated that more than six genes underlie this disorder. In addition, copy number v...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Li JJ,Chen YQ,Fan LL,Jin JY,Guo S,Xiang R

    更新日期:2018-02-01 00:00:00

  • LNA probe-based assay for the detection of Tomato black ring virus isolates.

    abstract::Tomato black ring virus (TBRV) infects a wide range of economically important plant species worldwide. In the present study we developed a locked nucleic acid (LNA) real-time RT-PCR assay for accurate detection of genetically diverse TBRV isolates collected from different hosts. The assay based on the LNA probe has a ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hasiów-Jaroszewska B,Rymelska N,Borodynko N

    更新日期:2015-02-01 00:00:00

  • Mutation screening for Japanese Lafora's disease patients: identification of novel sequence variants in the coding and upstream regulatory regions of EPM2A gene.

    abstract::The progressive myoclonus epilepsy of Lafora type (LD) is an autosomal recessive disorder caused by mutations in the EPM2A gene. We demonstrated recently that EPM2A encodes a dual-specificity phosphatase that is primarily associated with polyribosomes. In the present study, we screened for mutations in the EPM2A gene ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ganesh S,Shoda K,Amano K,Uchiyama A,Kumada S,Moriyama N,Hirose S,Yamakawa K

    更新日期:2001-10-01 00:00:00

  • Development and validation of a multiplex real-time PCR for detection of Clostridium chauvoei and Clostridium septicum.

    abstract::Clostridium chauvoei is the causative agent of blackleg in cattle and sheep. The clinical symptoms of this severe disease are very similar to that of malignant edema (Clostridium septicum), infections of other Clostridium species belonging to the gas edema complex, and anthrax (Bacillus anthracis). C. chauvoei and C. ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lange M,Neubauer H,Seyboldt C

    更新日期:2010-08-01 00:00:00

  • Multiplex PCR detection of Campylobacter jejuni and Arcobacter butzleri in food products.

    abstract::Arcobacter is a recently described species, previously considered part of the Campylobacter family. A sensitive assay such as that provided by PCR could help to distinguish the closely related Arcobacter from Campylobacter. A PCR method to specifically detect both Campylobacter jejuni and Arcobacter butzleri in the sa...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Winters DK,Slavik MF

    更新日期:2000-04-01 00:00:00

  • Down-regulation of UBA6 exacerbates brain injury by inhibiting the activation of Notch signaling pathway to promote cerebral cell apoptosis in rat acute cerebral infarction model.

    abstract::This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

    更新日期:2020-10-01 00:00:00