Abstract:
:A random amplified polymorphic DNA (RAPD) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of Schistosoma japonicum from seven geographical locations (Sj1: Zhejiang; Sj2: Anhui; Sj3: Jiangxi; Sj4: Hunan; Sj5: Hubei; Sj6: Sichuan; Sj7: Yunnan) of the People's Republic of China. Distinct differences between some isolates were reproducibly detected in RAPD patterns produced using five of the primers. The analyses showed that both Sj6 and Sj7 were quite distinct genetically from Sj1-Sj5 based on the presence/absence of particular bands (A10-200 bp, A9-220 bp, B17-520 bp, P205-680 bp and P235-930 bp). These findings are in line with previous reports on the biological, biochemical, immunological and chemotherapeutic differences of S. japonicum from Sichuan and Yunnan compared with other geographical regions. The present study showed, based on RAPD profiles, that genetic differences exist within S. japonicum from mainland China. This finding may have important implications for studying the population biology, epidemiology and clinical forms of the disease in China, as well as for developing vaccines and diagnostic test systems.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Gasser RB,Bao-Zhen Q,Nansen P,Johansen MV,Bøgh Hdoi
10.1006/mcpr.1996.0048subject
Has Abstractpub_date
1996-10-01 00:00:00pages
353-8issue
5eissn
0890-8508issn
1096-1194pii
S0890-8508(96)90048-1journal_volume
10pub_type
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journal_title:Molecular and cellular probes
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