Immunoblot and enzyme-linked immunosorbent assays of Campylobacter major outer-membrane protein and application to the differentiation of Campylobacter species.

abstract：:The development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay specifically detected PEDV and PCV3, with no fluorescence detected for oth...

journal_title：Molecular and cellular probes

authors： Han HY,Zheng HH,Zhao Y,Tian RB,Xu PL,Hou HL,Chen HY,Yang MF

更新日期：2019-04-01 00:00:00

abstract：BACKGROUND:Stroke is a leading cause of mortality worldwide. Rac-MAPK kinase 6 (Map2k6) plays important roles in cell proliferation and apoptosis. However, the role played by Map2k6 in stroke injury and the underlying mechanism of action remain unknown. METHODS:Mice received cerebral ischemia/reperfusion (I/R) injurie...

journal_title：Molecular and cellular probes

authors： Tang C,Ou J,Kou L,Deng J,Luo S

更新日期：2020-02-01 00:00:00

abstract：:The flagellin genes of four Burkholderia pseudomallei and two Burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (PCR)-based isolation method using the same pair of primers. The PCR-amplification products of the isolates showed a single band of about 1.1 kb, which is similar to a type I...

journal_title：Molecular and cellular probes

authors： Tungpradabkul S,Wajanarogana S,Tunpiboonsak S,Panyim S

更新日期：1999-04-01 00:00:00

abstract：:Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasm...

journal_title：Molecular and cellular probes

authors： Wang H,Fadl AA,Khan MI

更新日期：1997-06-01 00:00:00

abstract：:This study evaluates five cryptic plasmid-derived DNA probes in a 4-h slot-blot hybridization assay for the detection of Chlamydia trachomatis in cultures and clinical specimens. The probes, consisting of either the entire cloned 7.5 kbp cryptic plasmid pSE8 or one of four Hin dIII/Eco RI fragments measuring 710, 1055...

journal_title：Molecular and cellular probes

authors： Cano RJ,Murrieta CM,Spaulding DC,Pascual A

更新日期：1991-12-01 00:00:00

abstract：:Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific prim...

journal_title：Molecular and cellular probes

authors： Wan C,Cheng L,Chen C,Liu R,Shi S,Fu G,Chen H,Fu Q,Huang Y

更新日期：2019-10-01 00:00:00

abstract：:Microsatellites have recently been used for linkage analysis of genetic diseases and for DNA fingerprinting in forensic medicine. In the present study the heterozygosity, PIC values and allele distributions of four microsatellites, D8S85, D8S88, D5S346 and D7S460, in an Italian population have been investigated. After...

journal_title：Molecular and cellular probes

authors： Sirchia SM,Garagiola I,De Andreis C,Gazzoli I,Gramegna M,Colucci G

更新日期：1996-04-01 00:00:00

abstract：:We have developed a new methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase (TdT) using a thioflavin T probe. This method is highly selective and sensitive. The fluorescence intensity was direct proportion to Dam MTase concentration in...

journal_title：Molecular and cellular probes

authors： Ma C,Liu H,Li W,Chen H,Jin S,Wang J,Wang J

更新日期：2016-04-01 00:00:00

abstract：:Species identification of Mycobacterium tuberculosis remains a cumbersome process. We have developed a simple method for treating clinical samples which permits direct polymerase chain reaction (PCR) amplification of mycobacterial target DNA without organic extraction. Samples were boiled for 30 min in TE-Triton, then...

journal_title：Molecular and cellular probes

authors： Sritharan V,Barker RH Jr

更新日期：1991-10-01 00:00:00

abstract：:5-Fluorouracil (5FU), an antimetabolite often used for the treatment of breast cancer, binds to and inactivates the enzyme thymidylate synthase (TS). Measurement of TS levels may be useful in examining resistance to 5FU, but current methods involving ligand binding assays present considerable problems due to the low b...

journal_title：Molecular and cellular probes

authors： Elledge RM,Okuyama N,Fitzgerald SD,Fugua SA

更新日期：1994-02-01 00:00:00

abstract：:Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 196...

journal_title：Molecular and cellular probes

authors： Komissarov A,Fadeev A,Kosheleva A,Sintsova K,Grudinin M

更新日期：2017-10-01 00:00:00

abstract：:The detection limits of the polymerase chain reaction (PCR) for Mycoplasma pneumoniae were determined using specimens from persons known to have had M. pneumoniae pneumonia. Four primers were selected from the known sequence of the P1 gene. The primer pair (P1-178 and P1-809) which generates a 631 fragment gave the lo...

journal_title：Molecular and cellular probes

authors： Leng Z,Kenny GE,Roberts MC

更新日期：1994-04-01 00:00:00

abstract：:The single nucleotide polymorphism (SNP) genotyping is currently considered as a particularly valuable tool for the diagnosis of different pathologies. For this reason, over the past several years a great deal of effort has been devoted to developing accurate, rapid, and cost-effective technologies for SNP analysis. A...

journal_title：Molecular and cellular probes

authors： Etlik O,Koksal V,Arican-Baris ST,Baris I

更新日期：2011-08-01 00:00:00

abstract：:At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed...

journal_title：Molecular and cellular probes

authors： Newton LA,Chilton NB,Monti JR,Bjørn H,Várady M,Christensen CM,Gasser RB

更新日期：1997-04-01 00:00:00

abstract：:Although large expansions of the non-coding GGGGCC repeat in C9orf72 gene are clearly defined as pathogenic for Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Lobar Degeneration (FTLD), intermediate-length expansions have also been associated with those and other neurodegenerative diseases. Intermediate-length...

journal_title：Molecular and cellular probes

authors： Biasiotto G,Archetti S,Di Lorenzo D,Merola F,Paiardi G,Borroni B,Alberici A,Padovani A,Filosto M,Bonvicini C,Caimi L,Zanella I

更新日期：2017-04-01 00:00:00

abstract：:We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant d...

journal_title：Molecular and cellular probes

authors： Hong SH,Song J,Kim JQ

更新日期：2001-04-01 00:00:00

abstract：:A simple assay format was developed for the direct detection of C. trachomatis rRNA utilizing ligation of recombinant MDV-1 probe RNA fragments hybridized to 23S rRNA after capture and release from a solid support. Assay background (equivalent to 10(4) targets) was suppressed by blocking sequences in the 5' MDV report...

journal_title：Molecular and cellular probes

authors： Stefano JE,Genovese L,An Q,Lu L,McCarty J,Du Y,Stefano K,Burg JL,King W,Lane DJ

更新日期：1997-12-01 00:00:00

abstract：:A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis. ...

journal_title：Molecular and cellular probes

authors： Ren H,Yang M,Zhang G,Liu S,Wang X,Ke Y,Du X,Wang Z,Huang L,Liu C,Chen Z

更新日期：2016-04-01 00:00:00

abstract：:The RNA genome of pathogenic and non-pathogenic variants of citrus Hop stunt viroid (HSVd) differ by five to six nucleotides located within the variable (V) domain referred to as the "cachexia expression motif". Sensitive hosts such as mandarin and its hybrids are seriously affected by cachexia disease. Current method...

journal_title：Molecular and cellular probes

authors： Loconsole G,Onelge N,Yokomi RK,Kubaa RA,Savino V,Saponari M

更新日期：2013-10-01 00:00:00

abstract：:Quantification of nucleic acids, especially of mRNA, is increasingly important in biomedical research. The recently developed quantitative real-time polymerase chain reaction (PCR) - a highly sensitive technology for the rapid, accurate and reproducible quantification of gene expression - offers major advantages over ...

journal_title：Molecular and cellular probes

authors： Fronhoffs S,Totzke G,Stier S,Wernert N,Rothe M,Brüning T,Koch B,Sachinidis A,Vetter H,Ko Y

更新日期：2002-04-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a prevalent pathogen worldwide and causes various respiratory infections. Although it is a critical pathogen in pediatric patients, it is unclear how it enters host cells. In this study, we focused on hMPV cell entry using two kinds of cell lines (Vero E6 and LLC-MK2), which are most co...

journal_title：Molecular and cellular probes

authors： Yang H,He H,Tan B,Liu E,Zhao X,Zhao Y

更新日期：2016-08-01 00:00:00

abstract：:The ligase detection reaction (LDR) is a highly specific genotyping method for single nucleotide variations. Although LDR typically discriminates single nucleotide polymorphism (SNP) alleles at the 3' end of so-called LDR discriminating probes, we designed probes in which the position of nucleotide differences for dis...

journal_title：Molecular and cellular probes

authors： Asari M,Omura T,Maseda C,Shiono H,Tasaki Y,Matsubara K,Shimizu K

更新日期：2010-12-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) is responsible for severe infections in immunocompromised patients. Viral load has recently been identified as one of the major risk factors for subsequent development of HCMV disease. In this context, we developed a protocol allowing rapid, sensitive and precise quantification of HCMV DNA...

journal_title：Molecular and cellular probes

authors： Poirier-Toulemonde AS,Imbert-Marcille BM,Ferré-Aubineau V,Besse B,Le Roux MG,Cantarovich D,Billaudel S

更新日期：1997-02-01 00:00:00

abstract：:Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myoc...

journal_title：Molecular and cellular probes

authors： Xia X,Liang Y,Zheng W,Lin D,Sun S

更新日期：2020-08-01 00:00:00

abstract：:New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env...

journal_title：Molecular and cellular probes

authors： Sabunciyan S,Mandelberg N,Rabkin CS,Yolken R,Viscidi R

更新日期：2011-04-01 00:00:00

abstract：:An alkaline phosphatase (AP)-labeled genus-specific oligonucleotide probe was developed to detect and enumerate vibrios in shrimp larvae and their surrounding environment. The probe was evaluated using 35 laboratory isolates of Vibrio species and 29 isolates of non-vibrio species. The probe was specific for the Vibrio...

journal_title：Molecular and cellular probes

authors： Raghunath P,Karunasagar I,Karunasagar I

更新日期：2007-08-01 00:00:00

abstract：:Beak atrophy and dwarfism syndrome (BADS) is commonly caused by co-infection with duck circovirus (DuCV) and novel goose parvovirus (NGPV). Therefore, concurrent detection of both viruses is important for monitoring and limiting BADS, although such a diagnostic test has not been reported. In this study, we developed a...

journal_title：Molecular and cellular probes

authors： Wang Y,Cui Y,Li Y,Jiang S,Liu H,Wang J,Li Y

更新日期：2020-10-01 00:00:00

abstract：:Protein tyrosine phosphatase, non-receptor type 14 (PTPN14) exerts a profound effect in the progression of multiple malignant tumors. However, whether PTPN14 plays a role in prostate cancer has not been well investigated. Herein, we evaluated the function and potential underlying mechanism of PTPN14 in prostate cancer...

journal_title：Molecular and cellular probes

authors： Wang R,Du Y,Shang J,Dang X,Niu G

更新日期：2020-10-01 00:00:00

abstract：:The aim of the present study was to evaluate a mixed phase DNA hybridization assay for detection of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal aspirates from patients with suspected pertussis. Among 179 consecutive patients with own or parental suspicion of pertussis, the diagnosis was confirm...

journal_title：Molecular and cellular probes

authors： Reizenstein E,Morfeldt E,Granström G,Granström M,Löfdahl S

更新日期：1990-08-01 00:00:00

abstract：:Polymorphisms (rs1801282, rs8192678, rs7903146) of peroxisome proliferator-activated receptor gamma (PPARG), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PPARGC1A) and transcription factor 7-like 2 (TCF7L2) have recently been associated with different diseases, mainly type 2 diabetes. An assay...

journal_title：Molecular and cellular probes

authors： Habalová V,Klimcáková L,Zidzik J,Tkác I

更新日期：2009-02-01 00:00:00