当前位置： SCI文献检索 > MOLECULAR AND CELLULAR PROBES期刊下所有文献 > Evaluation of thymidylate synthase RNA expression by polymerase chain reaction.

Evaluation of thymidylate synthase RNA expression by polymerase chain reaction.

Abstract：

:5-Fluorouracil (5FU), an antimetabolite often used for the treatment of breast cancer, binds to and inactivates the enzyme thymidylate synthase (TS). Measurement of TS levels may be useful in examining resistance to 5FU, but current methods involving ligand binding assays present considerable problems due to the low basal level of this enzyme in many tissues. Therefore, a semiquantitative polymerase chain reaction (PCR) technique to measure TS RNA concentration in breast tumour cell lines has been developed with potential for measuring TS RNA levels in individual breast tumours. Using less than 1 microgram of total RNA, we determined that the T47D breast cancer cell line expressed TS levels 1.3 to 4.4 times lower than those of four other breast cancer cell lines, MCF-7, MDA-MB-468, MDA-MB-231, and MDA-A1R. Using the MCF-7 cell line as a representative of relatively high TS expression, the relationship between the S-phase fraction (SPF), and TS levels in MCF-7 and T47D cells was next examined because cell cycle kinetics can also influence drug response. TS expression was directly related to SPF. MCF-7 cells had a higher SPF, corresponding to their higher TS level relative to T47D cells. Lastly, 5FU sensitivity was assayed for and it was found that MCF-7 cells were more sensitive to 5FU than T47D, despite having higher TS levels. A semiquantitative technique for assessing TS RNA expression by PCR in breast cancer cell lines has been demonstrated. In the cell lines tested, there was no direct relationship between TS level and 5FU sensitivity, though there was a direct relationship between cell proliferation and sensitivity to 5FU.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Elledge RM,Okuyama N,Fitzgerald SD,Fugua SA

doi

10.1006/mcpr.1994.1010

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

67-72

issue

1

eissn

0890-8508

issn

1096-1194

pii

S0890850884710103

journal_volume

8

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR PROBES文献大全
  • A fluorescent method for detecting low-grade 11patUPD mosaicism in Beckwith-Wiedemann syndrome.

    abstract：:The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2003.07.002

    authors： Russo S,Mencarelli M,Cavalleri F,Selicorni A,Cogliati F,Larizza L

    更新日期：2003-12-01 00:00:00

  • Circ_016719 plays a critical role in neuron cell apoptosis induced by I/R via targeting miR-29c/Map2k6.

    abstract：BACKGROUND:Stroke is a leading cause of mortality worldwide. Rac-MAPK kinase 6 (Map2k6) plays important roles in cell proliferation and apoptosis. However, the role played by Map2k6 in stroke injury and the underlying mechanism of action remain unknown. METHODS:Mice received cerebral ischemia/reperfusion (I/R) injurie...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.101478

    authors： Tang C,Ou J,Kou L,Deng J,Luo S

    更新日期：2020-02-01 00:00:00

  • Selective growth of mosaic cells in chromosomal analysis of chorionic villi by conventional karyotyping.

    abstract：:The major cause of first-trimester pregnancy loss is chromosomal abnormality, which could be detected by many methods. Conventional karyotyping based on chorionic villi (CV) culture is frequently used but may have limitations due to culture failure and selective growth of cells. In this study, we aimed to investigate ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101532

    authors： Zhang Y,Lei Q,Liu J,Lin M,Luo L,Li T,Wang Q,Zhou C

    更新日期：2020-06-01 00:00:00

  • The construction and use of a PCR internal control.

    abstract：:An example of the application and contruction of a polymerase chain reaction (PCR) internal control is presented. The internal control is synthesized in one PCR reaction. The primers used in this reaction possess 5' over-hanging ends which are identical to the primers used in the diagnostic reaction, whereas their 3' ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1998.0170

    authors： Sachadyn P,Kur J

    更新日期：1998-10-01 00:00:00

  • Development of a p28-based PCR assay for Ehrlichia chaffeensis.

    abstract：:Detection of Ehrlichia chaffeensis is necessary to study interactions between the parasite and its vertebrate and invertebrate hosts. The purpose of this study was to develop a sensitive, specific PCR assay for E. chaffeensis based on the outer membrane protein gene, p28. Candidate primer sets were identified and rank...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2003.10.002

    authors： Wagner ER,Bremer WG,Rikihisa Y,Ewing SA,Needham GR,Unver A,Wang X,Stich RW

    更新日期：2004-04-01 00:00:00

  • Rapid PCR-based delineation of the porcine nodular worms, Oesophagostomum dentatum and O. quadrispinulatum.

    abstract：:At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1997.0097

    authors： Newton LA,Chilton NB,Monti JR,Bjørn H,Várady M,Christensen CM,Gasser RB

    更新日期：1997-04-01 00:00:00

  • Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism.

    abstract：:We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run usin...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2008.09.003

    authors： Gromadzka B,Smietanka K,Dragun J,Minta Z,Gora-Sochacka A,Szewczyk B

    更新日期：2008-10-01 00:00:00

  • Development of TaqMan RT-qPCR for the detection of type A human respiratory syncytial virus.

    abstract：:The human respiratory syncytial virus is a common respiratory pathogen in children. Improved diagnosis of the virus is dependent on the development of tools for the rapid detection and estimation of the viral loads. In the current study, RT-qPCR using TaqMan hydrolysis probe based on the F gene detection was developed...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2017.02.006

    authors： Abdel-Moneim AS,Shehab GM,Alsulaimani AA,Al-Malky MIR

    更新日期：2017-06-01 00:00:00

  • Molecular diagnosis of genital HPV DNA types by polymerase chain reaction and sensitivity-standardized filter in situ hybridization in randomly sampled cohorts of Singapore women.

    abstract：:Infection of the cervix uteri with various types of human papillomaviruses is generally considered a necessary factor in the etiology of cancer of the cervix uteri. In many human populations throughout the world, approximately 90% of cervical carcinomas are found to harbour HPV genomes, as judged by Southern blot hybr...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(90)90013-p

    authors： Chow V,Tham KM,Yeo-Gloss M,Lim-Tan SK,Sng I,Thirumoorthy T,Bernard HU

    更新日期：1990-04-01 00:00:00

  • A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR.

    abstract：:Species identification of Mycobacterium tuberculosis remains a cumbersome process. We have developed a simple method for treating clinical samples which permits direct polymerase chain reaction (PCR) amplification of mycobacterial target DNA without organic extraction. Samples were boiled for 30 min in TE-Triton, then...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(06)80011-3

    authors： Sritharan V,Barker RH Jr

    更新日期：1991-10-01 00:00:00

  • Development and validation of a cost-effective in-house method, tetra-primer ARMS PCR assay, in genotyping of seven clinically important point mutations.

    abstract：:The single nucleotide polymorphism (SNP) genotyping is currently considered as a particularly valuable tool for the diagnosis of different pathologies. For this reason, over the past several years a great deal of effort has been devoted to developing accurate, rapid, and cost-effective technologies for SNP analysis. A...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2011.04.005

    authors： Etlik O,Koksal V,Arican-Baris ST,Baris I

    更新日期：2011-08-01 00:00:00

  • Down-regulation of UBA6 exacerbates brain injury by inhibiting the activation of Notch signaling pathway to promote cerebral cell apoptosis in rat acute cerebral infarction model.

    abstract：:This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101612

    authors： Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

    更新日期：2020-10-01 00:00:00

  • Alternate PCR assays for screening of JH1 mutation associated with embryonic death in Jersey cattle.

    abstract：:Jersey haplotype (JH) 1, a stop-gain lethal mutation in the CWC15 gene, causes embryonic losses in Jersey cattle. Two PCR based assays using Amplification Refractory Mutation System (T-ARMS-PCR) and restriction fragment length polymorphism (PCR-RFLP) were developed for screening of the JH1 in cattle. During the screen...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101688

    authors： Kumar A,Gupta ID,Mohan G,M R V,D RK,S J,Kataria RS,Niranjan SK

    更新日期：2020-12-03 00:00:00

  • A new method for species identification and differentiation of Mycobacterium chelonae complex based on amplified hsp65 restriction analysis (AHSPRA).

    abstract：:Members of the Mycobacterium chelonae complex (MCC), namely M. chelonae, Mycobacterium abscessus and Mycobacterium immunogenum, have been implicated in nosocomial infections and occupational respiratory illnesses like hypersensitivity pneumonitis (HP) associated with contaminated metalworking fluid (MWF) exposures. Cl...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2004.09.007

    authors： Selvaraju SB,Khan IU,Yadav JS

    更新日期：2005-04-01 00:00:00

  • Rapid genomic typing of BK virus directly from clinical specimens.

    abstract：:A simple and rapid method, PCR-restriction enzyme analysis (PCR-RE) for BK virus (BKV) typing was developed, based on the presence of type-specific restriction enzyme sites in a 327 bp PCR-generated fragment which partially encodes the VP1 protein. The enzymes, Alu I, Xmn I and Ava II, were used to digest the PCR prod...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1993.1047

    authors： Jin L

    更新日期：1993-08-01 00:00:00

  • Human metapneumovirus uses endocytosis pathway for host cell entry.

    abstract：:Human metapneumovirus (hMPV) is a prevalent pathogen worldwide and causes various respiratory infections. Although it is a critical pathogen in pediatric patients, it is unclear how it enters host cells. In this study, we focused on hMPV cell entry using two kinds of cell lines (Vero E6 and LLC-MK2), which are most co...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2016.06.003

    authors： Yang H,He H,Tan B,Liu E,Zhao X,Zhao Y

    更新日期：2016-08-01 00:00:00

  • Direct detection of vanA and vanB genes in clinical specimens for rapid identification of vancomycin resistant enterococci (VRE) using multiplex PCR.

    abstract：:Surveillance for vancomycin resistant enterococci (VRE) by culture can be labour intensive and time consuming. We have developed a multiplex polymerase chain reaction (MPCR) which can be performed directly on the clinical specimen. The assay allows sensitive detection of enterococci with vanA - and vanB -mediated resi...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1999.0250

    authors： Petrich AK,Luinstra KE,Groves D,Chernesky MA,Mahony JB

    更新日期：1999-08-01 00:00:00

  • PRPF4 is a novel therapeutic target for the treatment of breast cancer by influencing growth, migration, invasion, and apoptosis of breast cancer cells via p38 MAPK signaling pathway.

    abstract：:Pre-mRNA processing factor 4 (PRPF4), a core protein in U4/U6 snRNP, maintains snRNP structures by interacting with PRPF3 and cyclophilin H. Expression of the PRPF4 gene affects cell survival as well as apoptosis and is responsible for retinitis pigmentosa (RP). Proteomics analysis shows that PRPF4 may be a therapeuti...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.101440

    authors： Park S,Han SH,Kim HG,Jeong J,Choi M,Kim HY,Kim MG,Park JK,Han JE,Cho GJ,Kim MO,Ryoo ZY,Choi SK

    更新日期：2019-10-01 00:00:00

  • Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis.

    abstract：:Beak atrophy and dwarfism syndrome (BADS) is commonly caused by co-infection with duck circovirus (DuCV) and novel goose parvovirus (NGPV). Therefore, concurrent detection of both viruses is important for monitoring and limiting BADS, although such a diagnostic test has not been reported. In this study, we developed a...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101648

    authors： Wang Y,Cui Y,Li Y,Jiang S,Liu H,Wang J,Li Y

    更新日期：2020-10-01 00:00:00

  • Potential regulatory SNPs in promoters of human genes: a systematic approach.

    abstract：:Single nucleotide polymorphisms (SNPs) can significantly contribute to the cellular level of the mRNA transcripts encoded by human disease related genes. DNA variations between individuals can be an indication of predisposition to disease or affect the response to treatment. An algorithm allowing in silico extraction ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2006.03.007

    authors： Stepanova M,Tiazhelova T,Skoblov M,Baranova A

    更新日期：2006-12-01 00:00:00

  • Development of PCR based assays for detection of lethal Holstein haplotype 1, 3 and 4 in Holstein Friesian cattle.

    abstract：:Holstein haplotype (HH) 1, 3 and 4 are lethal mutations, responsible for early embryonic losses in Holstein Friesian (HF) cattle, worldwide. Three PCR based assays - tetra Amplification Refractory Mutation System PCR, PCR primer induced restriction analysis and PCR-restriction fragment length polymorphism techniques f...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.101503

    authors： Kumar A,Gupta ID,Mohan G,Vineeth MR,Ravi Kumar D,Jayakumar S,Niranjan SK

    更新日期：2020-04-01 00:00:00

  • Rapid prediction of inducible clarithromycin resistance in Mycobacterium abscessus.

    abstract：:We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2015.08.007

    authors： Zhu YC,Mitchell KK,Nazarian EJ,Escuyer VE,Musser KA

    更新日期：2015-12-01 00:00:00

  • On-line moisture determination of ore concentrates 'a review of traditional methods and introduction of a novel solution'.

    abstract：:The manual gravimetric drying moisture determination methods currently employed by most mineral processing plants fail to provide timely and accurate information required for automatic control. The costs associated with transporting and handling concentrates still represent a major portion of the overall treatment pri...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章,评审

    doi：10.1006/mcpr.2002.0444

    authors： Cancilla PA,Barrette P,Rosenblum F

    更新日期：2002-12-01 00:00:00

  • Cloning of the antigen 85A from Mycobacterium gordonae and its use for the specific PCR identification of these mycobacteria.

    abstract：:The complete nucleotide sequence of 85A antigen of Mycobacterium gordonae was determined. This gene encodes 339 amino acids, including 43 amino acids for the signal peptide, followed by a mature protein of 296 amino acids. A polymerase chain reaction (PCR) assay for the rapid detection of M. gordonae DNA using two pai...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1997.0110

    authors： Dumonceaux M,Fauville Dufaux M,Ooms J,De Wit L,Sonck P,Content J

    更新日期：1997-08-01 00:00:00

  • Exploring transcriptional conservation between Ancylostoma caninum and Haemonchus contortus by oligonucleotide microarray and bioinformatic analyses.

    abstract：:In this study, we identified, using an established oligonucleotide microarray platform for the parasitic nematode Haemonchus contortus, transcripts that are 'conserved' between serum-activated and non-activated L3s of Ancylostoma caninum (aL3 and L3, respectively) and H. contortus by cross-species hybridization (CSH) ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2008.09.004

    authors： Cantacessi C,Loukas A,Campbell BE,Mulvenna J,Ong EK,Zhong W,Sternberg PW,Otranto D,Gasser RB

    更新日期：2009-02-01 00:00:00

  • Development of PCR method specific for Marek's disease virus.

    abstract：:A rapid polymerase chain reaction (PCR) assay specific for Marek's Disease Virus (MDV) was developed. This assay was able to detect MDV in inoculated chick kidney cells at dilutions of 10(-5). Negative PCR results were obtained using uninoculated chick cells, Marek's Disease Vaccine (SB), Herpesvirus of Turkeys (HVT) ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1993.1017

    authors： Rong-Fu W,Beasley JN,Cao WW,Slavik MF,Johnson MG

    更新日期：1993-04-01 00:00:00

  • Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

    abstract：:Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1994.1035

    authors： Axton RA,Brock DJ

    更新日期：1994-06-01 00:00:00

  • Endostar regulates EMT, migration and invasion of lung cancer cells through the HGF-Met pathway.

    abstract：AIM:Though Endostar (ES) could inhibit tumor growth by inhibiting tumor angiogenesis, other possible mechanisms have been less reported. This study aims to investigate the role of ES in the treatment of lung cancer from the perspective of macrophage-mediated epithelial mesenchymal transformation (EMT). METHODS:THP1 ce...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.05.003

    authors： Shen Y,Chen Q,Li L

    更新日期：2019-06-01 00:00:00

  • Molecular diagnostic tests to predict the risk of progressive multifocal leukoencephalopathy in natalizumab-treated multiple sclerosis patients.

    abstract：:Natalizumab is a humanized monoclonal antibody against the alpha4 chain of the alpha4beta1 and alpha4beta7 integrin heterodimers used with high effectiveness in the treatment of multiple sclerosis. The use of this drug can unfortunately be associated with the onset of progressive multifocal leukoencephalopathy, a poss...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章,评审

    doi：10.1016/j.mcp.2014.11.007

    authors： Rossi F,Newsome SD,Viscidi R

    更新日期：2015-02-01 00:00:00

  • Development of a realtime RT-PCR assay for the rapid detection of influenza A(H2) viruses.

    abstract：:Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 196...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2017.06.005

    authors： Komissarov A,Fadeev A,Kosheleva A,Sintsova K,Grudinin M

    更新日期：2017-10-01 00:00:00