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Development of PCR method specific for Marek's disease virus.

Abstract：

:A rapid polymerase chain reaction (PCR) assay specific for Marek's Disease Virus (MDV) was developed. This assay was able to detect MDV in inoculated chick kidney cells at dilutions of 10(-5). Negative PCR results were obtained using uninoculated chick cells, Marek's Disease Vaccine (SB), Herpesvirus of Turkeys (HVT) and Fowl Laryngotracheitis Vaccine (LT). Bursae, feathers and kidneys from MDV infected chickens were positive in the PCR assay. The same tissues from normal chickens were negative. This method required only 0.5 h for sample preparatory, 3 h for PCR application and 1 h for electrophoresis. Internal probe hybridization confirmed that the PCR products are from MDV, but this hybridization will not be necessary for future MDV detection.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Rong-Fu W,Beasley JN,Cao WW,Slavik MF,Johnson MG

doi

10.1006/mcpr.1993.1017

subject

Has Abstract

pub_date

1993-04-01 00:00:00

pages

127-31

issue

2

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(83)71017-0

journal_volume

7

pub_type

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