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Detection of human cytomegalovirus in cervicovaginal cells by culture, in situ DNA hybridization and DNA amplification methods.

Abstract：

:The presence of human cytomegalovirus (HCMV) was tested in 388 cervicovaginal cells specimens obtained from the same number of pregnant women. HCMV was detected in 5.41%, 11.6% and 13.9% of these specimens by conventional culture, in situ DNA hybridization and polymerase chain reaction (PCR) methods, respectively. The sensitivities of detecting HCMV by in situ hybridization and PCR methods were 76.2% and 90.5% and the specificities were 92.1% and 90.5%, respectively, when compared with conventional culture method. The PCR compared favourably with both conventional culture and in situ hybridization methods and it may become a valuable and useful tool for the early and rapid detection of HCMV in clinical specimens.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Yuan CF,Kao SM,Wang DC,Ng HT,Pao CC

doi

10.1016/0890-8508(90)90006-l

subject

Has Abstract

pub_date

1990-12-01 00:00:00

pages

475-83

issue

6

eissn

0890-8508

issn

1096-1194

journal_volume

4

pub_type

杂志文章

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