当前位置： SCI文献检索 > MOLECULAR AND CELLULAR PROBES期刊下所有文献 > Rapid control of purity for the cytoplasm of male-sterile seed stocks by means of a dot hybridization assay.

Rapid control of purity for the cytoplasm of male-sterile seed stocks by means of a dot hybridization assay.

Abstract：

:To produce hybrids, one member of the parental line is genetically made male-sterile. This male-sterile trait is encoded by mitochondria so that it is maternally inherited. Consequently, the progeny of a male-sterile plant is fully sterile. Nevertheless, during the handling of cytoplasmic male-sterile seed stocks, some mixture with seeds of the maintainer lines can occur. Up to the present time, the only way to check the homogeneity of the cytoplasmic male-sterile seed stock was to grow the plants until flowering time. We have developed a method which can be used immediately after the harvest, allowing us to check samples from both sunflower and sugar beet. We used the mitochondrial plasmid, present only in the maintainer lines, as a probe for the total nucleic acids prepared from the cytoplasmic male-sterile seed stocks which might be contaminated. The signals compared to those of samples artificially contaminated allow us to measure as few as one male-fertile seed in 1000 seeds in a rapid and accurate manner.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Santoni S,Faivre-Rampant P,Moreau E,Bervillé A

doi

10.1016/0890-8508(91)90032-f

subject

Has Abstract

pub_date

1991-02-01 00:00:00

pages

1-9

issue

1

eissn

0890-8508

issn

1096-1194

pii

0890-8508(91)90032-F

journal_volume

5

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR PROBES文献大全
  • Experiences on the application of the polymerase chain reaction in a diagnostic laboratory.

    abstract：:Double polymerase chain reaction (PCR) assays with nested primers have been applied in a routine laboratory for the diagnosis of herpes-, pesti- and retroviral infections of animals. Various methods and tools have been tested to prevent and to eliminate false positive results as well as to visualize the PCR products (...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章,评审

    doi：10.1006/mcpr.1993.1035

    authors： Belák S,Ballagi-Pordány A

    更新日期：1993-06-01 00:00:00

  • PCR-RFLP analysis of the flagellin sequences for identification of Burkholderia pseudomallei and Burkholderia cepacia from clinical isolates.

    abstract：:The flagellin genes of four Burkholderia pseudomallei and two Burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (PCR)-based isolation method using the same pair of primers. The PCR-amplification products of the isolates showed a single band of about 1.1 kb, which is similar to a type I...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1999.0221

    authors： Tungpradabkul S,Wajanarogana S,Tunpiboonsak S,Panyim S

    更新日期：1999-04-01 00:00:00

  • Evaluation of thymidylate synthase RNA expression by polymerase chain reaction.

    abstract：:5-Fluorouracil (5FU), an antimetabolite often used for the treatment of breast cancer, binds to and inactivates the enzyme thymidylate synthase (TS). Measurement of TS levels may be useful in examining resistance to 5FU, but current methods involving ligand binding assays present considerable problems due to the low b...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1994.1010

    authors： Elledge RM,Okuyama N,Fitzgerald SD,Fugua SA

    更新日期：1994-02-01 00:00:00

  • Detection and discrimination of B pertussis and B holmesii by real-time PCR targeting IS481 using a beacon probe and probe-target melting analysis.

    abstract：:A beacon probe was designed to detect one of the two documented single nucleotide changes in IS481 target allele of Bordetella holmesii genome as compared to Bordetella pertussis. PCR amplified product targeting a region of IS481 in presence of the probe was subjected to a post-PCR hybridization and melting cycle. Hyb...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(03)00026-4

    authors： Poddar SK

    更新日期：2003-04-01 00:00:00

  • A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter.

    abstract：:In a previous study, we reported the performance of a PCR assay amplifying 287-bp of the 16S rRNA gene of thermo-tolerant Campylobacter (C. jejuni, C. lari, C. coli) through an international ring-trial involving 12 participating laboratories. Based on the validated set of primers, a LightCycler real-time PCR assay (LC...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2004.04.005

    authors： Perelle S,Josefsen M,Hoorfar J,Dilasser F,Grout J,Fach P

    更新日期：2004-10-01 00:00:00

  • Impact of fixation artifacts and threshold selection on high resolution melting analysis for KRAS mutation screening.

    abstract：INTRODUCTION:Treatment in metastatic colorectal cancer (mCRC) has expanded with monoclonal antibodies targeting epidermal growth factor receptor, but is restricted to patients with a wild-type (WT) KRAS mutational status. The most sensitive assays for KRAS mutation detection in formalin-fixed paraffin embedded (FFPE) t...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2017.06.003

    authors： Pérez-Báez W,García-Latorre EA,Maldonado-Martínez HA,Coronado-Martínez I,Flores-García L,Taja-Chayeb L

    更新日期：2017-10-01 00:00:00

  • Immunoblot and enzyme-linked immunosorbent assays of Campylobacter major outer-membrane protein and application to the differentiation of Campylobacter species.

    abstract：:The major outer-membrane protein (MOMP) from Campylobacter jejuni NCTC 11168 was purified by solubilization in Triton X-100. Whole-cell proteins of Campylobacter species and the MOMP were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linke...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(87)90038-7

    authors： Taylor DE,Chang N

    更新日期：1987-09-01 00:00:00

  • A DNA microarray for identification of virulence and antimicrobial resistance genes in Salmonella serovars and Escherichia coli.

    abstract：:Characterization of antimicrobial resistance and virulence gene profiles provides important information on the potential pathogenicity of bacteria. This information can be used to facilitate prompt and effective treatment of bacterial infections. We developed and tested a PCR-based microarray platform for detecting vi...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2004.11.008

    authors： Chen S,Zhao S,McDermott PF,Schroeder CM,White DG,Meng J

    更新日期：2005-06-01 00:00:00

  • Integrated multiplex ligation dependent probe amplification (MLPA) assays for the detection of alterations in the HEXB, GM2A and SMARCAL1 genes to support the diagnosis of Morbus Sandhoff, M. Tay-Sachs variant AB and Schimke immuno-osseous dysplasia in hu

    abstract：:Multiplex ligation dependent probe amplification (MLPA) assays were designed for the genes HEXB (OMIM: 606873), GM2A (OMIM: 613109) and SMARCAL1 (OMIM: 606622) of humans. Two sets of synthetic MLPA probes for these coding exons were tested. Changes in copy numbers were detected as well as single nucleotide polymorphis...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2012.08.007

    authors： Sobek AK,Evers C,Dekomien G

    更新日期：2013-02-01 00:00:00

  • Mutation screening of the chromosome 8q24.3-human activity-regulated cytoskeleton-associated gene (ARC) in idiopathic generalized epilepsy.

    abstract：:Idiopathic generalized epilepsy (IGE) comprises a heterogeneous group of disorders, in which a high genetic predisposition and a complex mode of inheritance have been suggested. However, genes, which confer liability to common IGE subtypes including juvenile myoclonic epilepsy (JME) and childhood absence epilepsy (CAE...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2000.0314

    authors： Haug K,Kremerskothen J,Hallmann K,Sander T,Dullinger J,Rau B,Beyenburg S,Lentze MJ,Barnekow A,Elger CE,Propping P,Heils A

    更新日期：2000-08-01 00:00:00

  • Endostar regulates EMT, migration and invasion of lung cancer cells through the HGF-Met pathway.

    abstract：AIM:Though Endostar (ES) could inhibit tumor growth by inhibiting tumor angiogenesis, other possible mechanisms have been less reported. This study aims to investigate the role of ES in the treatment of lung cancer from the perspective of macrophage-mediated epithelial mesenchymal transformation (EMT). METHODS:THP1 ce...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.05.003

    authors： Shen Y,Chen Q,Li L

    更新日期：2019-06-01 00:00:00

  • Label-free monitoring of DNA methyltransferase activity based on terminal deoxynucleotidyl transferase using a thioflavin T probe.

    abstract：:We have developed a new methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase (TdT) using a thioflavin T probe. This method is highly selective and sensitive. The fluorescence intensity was direct proportion to Dam MTase concentration in...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2016.02.001

    authors： Ma C,Liu H,Li W,Chen H,Jin S,Wang J,Wang J

    更新日期：2016-04-01 00:00:00

  • Down-regulation of UBA6 exacerbates brain injury by inhibiting the activation of Notch signaling pathway to promote cerebral cell apoptosis in rat acute cerebral infarction model.

    abstract：:This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101612

    authors： Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

    更新日期：2020-10-01 00:00:00

  • High-throughput qualitative multiplex 5' nuclease assay using post-only PCR analysis.

    abstract：:Homogenous fluorescence PCR assays offer distinct advantages for qualitative testing and are gaining immense popularity in fields like diagnostic microbiology. To meet the demand of high-volume laboratories, we developed a protocol for qualitative multiplex 5' nuclease assays using post-only PCR analysis. This novel a...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2005.06.001

    authors： Antonishyn NA,McDonald RR,Chan EL,Horsman GB

    更新日期：2005-10-01 00:00:00

  • Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.

    abstract：:A simple dual-label hybridization test for normal and mutant cystic fibrosis (CF) alleles is described. The assay is based on time-resolved fluorometry (TRF), which allows the simultaneous detection of DNA probes labelled with different lanthanides from one hybridization reaction. DNA was liberated from dried blood di...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(92)90047-2

    authors： Iitiä A,Liukkonen L,Siitari H

    更新日期：1992-12-01 00:00:00

  • Multiple paternity and sperm storage in captive Hermann's tortoises, Testudo hermanni boettgeri determined from amniotic fluid adhering to the eggshell.

    abstract：:We identified multiple paternity in 52.9% of the clutches of Hermann's tortoise Testudo hermanni boettgeri using polymorphic microsatellite markers. In addition we demonstrated sperm storage across seasons. DNA was extracted from the amniotic fluid adhering to the eggshell's inner surface, a procedure suitable for eas...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2015.05.009

    authors： Farke CM,Olek K,Gerding WM,Distler C

    更新日期：2015-08-01 00:00:00

  • Development of a SYBR green I-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine epidemic diarrhea virus and porcine circovirus 3.

    abstract：:The development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay specifically detected PEDV and PCV3, with no fluorescence detected for oth...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.02.002

    authors： Han HY,Zheng HH,Zhao Y,Tian RB,Xu PL,Hou HL,Chen HY,Yang MF

    更新日期：2019-04-01 00:00:00

  • Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66.

    abstract：:Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three reactions, through SYBR green I melting curve analysis. The method utilizes type-specific primer se...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2014.09.003

    authors： Tsakogiannis D,Papacharalampous M,Toska E,Kyriakopoulou Z,Dimitriou TG,Ruether IG,Komiotis D,Markoulatos P

    更新日期：2015-02-01 00:00:00

  • Quantification of the detection of Pneumocystis carinii by DNA amplification.

    abstract：:We have developed a highly specific and sensitive technique for the detection of Pneumocystis carinii DNA using DNA amplification by the polymerase chain reaction (PCR). PCR products are detected by agarose gel electrophoresis and Southern hybridization to an oligonucleotide probe. Here we report the calibration of pa...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(92)90055-3

    authors： Peters SE,Wakefield AE,Banerji S,Hopkin JM

    更新日期：1992-04-01 00:00:00

  • Molecular contrast of EGFR expression using gold nanoparticles as a reflectance-based imaging probe.

    abstract：:Advanced reflectance-based optical techniques for in vivo imaging often suffer from low contrast between neoplastic and normal tissue and are unable to image early biomolecular changes associated with carcinogenesis, thus limiting their clinical value. In this study, we exploit the resonance light scattering property ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2007.06.010

    authors： Kah JC,Olivo MC,Lee CG,Sheppard CJ

    更新日期：2008-02-01 00:00:00

  • MiR-34a suppresses the proliferation and invasion of gastric cancer by modulating PDL1 in the immune microenvironment.

    abstract：OBJECTIVES:As one of the most serious malignant carcinomas that threaten the life of sufferers constantly, gastric cancer has attracted a lot of interest among researchers. miR-34a, a member of hundreds of microRNAs (miRNAs), has been elucidated to exert a suppressive role in gastric cancer tumorgenesis based on previo...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101601

    authors： Yong H,Fu J,Gao G,Shi H,Zheng D,Zhou X

    更新日期：2020-10-01 00:00:00

  • Multiplex PCR for avian pathogenic mycoplasmas.

    abstract：:Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasm...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1997.0108

    authors： Wang H,Fadl AA,Khan MI

    更新日期：1997-06-01 00:00:00

  • Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.

    abstract：:Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 's...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2010.07.002

    authors： Peruzzi B,Serra M,Pescucci C,Sica M,Lastraioli S,Rondelli T,Pedemonte S,Risitano AM,De Angioletti M,Piccioli P,Notaro R

    更新日期：2010-12-01 00:00:00

  • miR-410-5p promotes the development of diabetic cardiomyopathy by suppressing PIM1-induced anti-apoptosis.

    abstract：:Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myoc...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101558

    authors： Xia X,Liang Y,Zheng W,Lin D,Sun S

    更新日期：2020-08-01 00:00:00

  • Fluorescence-based DNA minisequence analysis for detection of known single-base changes in genomic DNA.

    abstract：:We describe a rapid, automated method for direct detection of known single-base changes in genomic DNA. Fluorescence-based DNA minisequence analysis is employed in a template-dependent reaction which involves a single nucleotide extension of an oligonucleotide primer by the correct fluorescently-tagged dideoxynucleoti...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1995.0027

    authors： Kobayashi M,Rappaport E,Blasband A,Semeraro A,Sartore M,Surrey S,Fortina P

    更新日期：1995-06-01 00:00:00

  • Rapid prediction of inducible clarithromycin resistance in Mycobacterium abscessus.

    abstract：:We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2015.08.007

    authors： Zhu YC,Mitchell KK,Nazarian EJ,Escuyer VE,Musser KA

    更新日期：2015-12-01 00:00:00

  • A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction.

    abstract：:Quantification of nucleic acids, especially of mRNA, is increasingly important in biomedical research. The recently developed quantitative real-time polymerase chain reaction (PCR) - a highly sensitive technology for the rapid, accurate and reproducible quantification of gene expression - offers major advantages over ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2002.0405

    authors： Fronhoffs S,Totzke G,Stier S,Wernert N,Rothe M,Brüning T,Koch B,Sachinidis A,Vetter H,Ko Y

    更新日期：2002-04-01 00:00:00

  • Cloning of the antigen 85A from Mycobacterium gordonae and its use for the specific PCR identification of these mycobacteria.

    abstract：:The complete nucleotide sequence of 85A antigen of Mycobacterium gordonae was determined. This gene encodes 339 amino acids, including 43 amino acids for the signal peptide, followed by a mature protein of 296 amino acids. A polymerase chain reaction (PCR) assay for the rapid detection of M. gordonae DNA using two pai...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1997.0110

    authors： Dumonceaux M,Fauville Dufaux M,Ooms J,De Wit L,Sonck P,Content J

    更新日期：1997-08-01 00:00:00

  • Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism.

    abstract：:We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run usin...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2008.09.003

    authors： Gromadzka B,Smietanka K,Dragun J,Minta Z,Gora-Sochacka A,Szewczyk B

    更新日期：2008-10-01 00:00:00

  • Development of a novel and rapid polymerase spiral reaction (PSR) assay to detect Salmonella in pork and pork products.

    abstract：:The polymerase spiral reaction (PSR), a novel isothermal method for targeted DNA amplification, was effectively applied to detect Salmonella in artificially spiked pork. The specificity of the developed PSR was tested using 16 Salmonella and 15 non-Salmonella strains. The PSR assay was 10-fold more sensitive than conv...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101510

    authors： Momin KM,Milton AAP,Ghatak S,Thomas SC,Priya GB,Das S,Shakuntala I,Sanjukta R,Puro KU,Sen A

    更新日期：2020-04-01 00:00:00