Down-regulation of UBA6 exacerbates brain injury by inhibiting the activation of Notch signaling pathway to promote cerebral cell apoptosis in rat acute cerebral infarction model.


:This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-regulated overlapping DEGs and 51 down-regulated overlapping DEGs were used to construct the PPI network by STRING online database. UBA6 was identified as a hub gene by the CytoHubba plugin from Cytoscape. GO and KEGG pathway enrichment analyses were conducted using DAVID online website. UBA6 knockout exacerbated MCAO-mediated brain injury and cell apoptosis in rat brain tissues by H&E and TTC staining and TUNEL assay. The results of flow cytometry and western blot assays further demonstrated that UBA6 inhibition induced the apoptosis of hippocampal neurons and increased cleaved-caspase-3/9 protein levels. Notch1, NICD and Hes1 protein levels were suppressed by down-regulated UBA6. UBA6 was lowly expression in poor prognosis group of 100 patients with ACI. Logistic regression analysis indicated that hypertension, blood glucose, urokinase dose, UBA6 expression and AF were the main risk factors of poor prognosis after thrombolytic therapy for patients with ACI. The ROC curve analysis showed that the sensitivity and specificity of UBA6 was good (sensitivity 100%, specificity 89%, and AUC = 0.772) to be used to evaluate the poor prognosis of ACI. In conclusion, down-regulated UBA6 intensified MCAO-induced brain injury by inhibiting the activation of Notch signaling pathway to promote the apoptosis of hippocampal neurons and was used to predict the poor prognosis of ACI.


Mol Cell Probes


Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q




Has Abstract


2020-10-01 00:00:00












  • Evaluation of thymidylate synthase RNA expression by polymerase chain reaction.

    abstract::5-Fluorouracil (5FU), an antimetabolite often used for the treatment of breast cancer, binds to and inactivates the enzyme thymidylate synthase (TS). Measurement of TS levels may be useful in examining resistance to 5FU, but current methods involving ligand binding assays present considerable problems due to the low b...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Elledge RM,Okuyama N,Fitzgerald SD,Fugua SA

    更新日期:1994-02-01 00:00:00

  • Rapid diagnosis of spinal muscular atrophy using tetra-primer ARMS PCR assay: simultaneous detection of SMN1 and SMN2 deletion.

    abstract::Spinal muscular atrophy (SMA), the leading genetic cause of death in childhood, is an autosomal recessive neuromuscular disorder characterized by progressive muscle weakness, associated with deletions of the survival motor neuron 1 (SMN1) gene. Approximately 94% of SMA patients carry homologous deletions of SMN1 exon(...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Baris I,Etlik O,Koksal V,Arican-Baris ST

    更新日期:2010-06-01 00:00:00

  • Development of a pair of real-time loop mediated isothermal amplification assays for detection of Yersinia pestis, the causative agent of plague.

    abstract::Yersinia pestis, the causative agent of plague mainly infects rodents, while humans are the accidental host. The conventional diagnostic methods available for Y. pestis exhibit cross-reactivity with other enteropathogenic bacteria which makes its detection difficult. Rapid and reliable point-of-care detection of Y. pe...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Singh R,Pal V,Tripathi NK,Goel AK

    更新日期:2020-12-01 00:00:00

  • Detection of PCR products using PNA strand invasion.

    abstract::The unique ability of homopyrimidine peptide nucleic acid (PNA) to strand invade homopurine sites of duplex DNA offers a potential alternative to existing techniques for rapid detection of PCR products. From gel shift studies, PNA was found to specifically strand invade homopurine sites that had been incorporated into...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Drewe LJ,Brightwell G,Hall EA

    更新日期:2000-10-01 00:00:00

  • Polymorphism rs3737787 of Upstream Stimulatory Factor 1 gene is associated with serum lipid phenotype in Nigerian population.

    abstract::Serum lipid profile which is determined by genotype-phenotype relationship plays a significant role in the development of cardiovascular disease. Upstream stimulatory factor 1 (USF1), has been reported to be associated with serum lipid levels in different population, hence, this study investigated the association of v...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Onadeko OT,Okunowo WO,Imaga NOA,Abdulrazaq MM,Onuminya OJ,Van-Lare TO,Nwosu M

    更新日期:2020-12-08 00:00:00

  • PRPF4 is a novel therapeutic target for the treatment of breast cancer by influencing growth, migration, invasion, and apoptosis of breast cancer cells via p38 MAPK signaling pathway.

    abstract::Pre-mRNA processing factor 4 (PRPF4), a core protein in U4/U6 snRNP, maintains snRNP structures by interacting with PRPF3 and cyclophilin H. Expression of the PRPF4 gene affects cell survival as well as apoptosis and is responsible for retinitis pigmentosa (RP). Proteomics analysis shows that PRPF4 may be a therapeuti...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Park S,Han SH,Kim HG,Jeong J,Choi M,Kim HY,Kim MG,Park JK,Han JE,Cho GJ,Kim MO,Ryoo ZY,Choi SK

    更新日期:2019-10-01 00:00:00

  • Detection and molecular typing of Campylobacter jejuni in fecal samples by polymerase chain reaction.

    abstract::In order to determine whether polymerase chain reaction (PCR) could be used to detect Campylobacter jejuni directly in stool samples, DNA from 66 frozen culture positive and negative fecal samples was purified by column chromatography. The flaA gene was amplified using primers directed against the conserved 5' and 3' ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Waegel A,Nachamkin I

    更新日期:1996-04-01 00:00:00

  • Mutation screening for Japanese Lafora's disease patients: identification of novel sequence variants in the coding and upstream regulatory regions of EPM2A gene.

    abstract::The progressive myoclonus epilepsy of Lafora type (LD) is an autosomal recessive disorder caused by mutations in the EPM2A gene. We demonstrated recently that EPM2A encodes a dual-specificity phosphatase that is primarily associated with polyribosomes. In the present study, we screened for mutations in the EPM2A gene ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ganesh S,Shoda K,Amano K,Uchiyama A,Kumada S,Moriyama N,Hirose S,Yamakawa K

    更新日期:2001-10-01 00:00:00

  • Characterization of four microsatellites in an Italian population and their application to paternity testing.

    abstract::Microsatellites have recently been used for linkage analysis of genetic diseases and for DNA fingerprinting in forensic medicine. In the present study the heterozygosity, PIC values and allele distributions of four microsatellites, D8S85, D8S88, D5S346 and D7S460, in an Italian population have been investigated. After...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sirchia SM,Garagiola I,De Andreis C,Gazzoli I,Gramegna M,Colucci G

    更新日期:1996-04-01 00:00:00

  • Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay.

    abstract::The aim of this study was the development of a real-time PCR for HIV DNA quantification in whole blood leucocytes providing an alternative assay to those already described, almost based on the gag gene detection. The technique (pbs-rtPCR assay) is more rapid (the whole assay required less than 5h), easy to perform, om...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Casabianca A,Gori C,Orlandi C,Forbici F,Federico Perno C,Magnani M

    更新日期:2007-10-01 00:00:00

  • Construction of an internal control for the detection of Chlamydia pneumoniae by PCR.

    abstract::For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ursi D,Ieven M,Van Bever HP,Goossens H

    更新日期:1998-08-01 00:00:00

  • Serogroup specific single and multiplex PCR with pre-enrichment culture and immuno-magnetic bead capture for identifying strains of D. nodosus in sheep with footrot prior to vaccination.

    abstract::The identification of Dichelobacter nodosus present in a flock is a prerequisite to specific (autogenous) vaccination. Current methods of identification of the serogroup present in a population requires that the organisms be isolated, identified visually in mixed culture on streak plates, subcultured to purify and sub...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Dhungyel OP,Whittington RJ,Egerton JR

    更新日期:2002-08-01 00:00:00

  • Rapid and visual detection of dengue virus using recombinase polymerase amplification method combined with lateral flow dipstick.

    abstract::Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

    更新日期:2019-08-01 00:00:00

  • A rapid one-tube PCR method for simultaneously differentiating homozygotes and heterozygotes of the Sp1 binding site polymorphism in collagen type Ialpha1.

    abstract::Rapid detection of single-base changes is fundamental to molecular medicine. PCR amplification of specific alleles (PASA) has previously been used as a rapid method of genotyping single-nucleotide changes, but one reaction is required for each allele. This paper describes a Bidirectional PASA (Bi-PASA) method, which w...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lundberg GA

    更新日期:2007-06-01 00:00:00

  • Comparison of multiplex PCR, PCR-ELISA and fluorogenic 5' nuclease PCR assays for detection of plasmid-bearing virulent Yersinia enterocolitica in swine feces.

    abstract::Swine are implicated as the principal animal reservoir for plasmid-bearing Yersinia enterocolitica (YEP(+)) strains that are pathogenic to humans. To evaluate the utility of the PCR for detection of YEP(+) strains in naturally-contaminated pig feces, samples were first enriched in Irgasan ticarcillin potassium chlorat...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bhaduri S

    更新日期:2002-06-01 00:00:00

  • Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.

    abstract::A simple dual-label hybridization test for normal and mutant cystic fibrosis (CF) alleles is described. The assay is based on time-resolved fluorometry (TRF), which allows the simultaneous detection of DNA probes labelled with different lanthanides from one hybridization reaction. DNA was liberated from dried blood di...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Iitiä A,Liukkonen L,Siitari H

    更新日期:1992-12-01 00:00:00

  • Rapid PCR using nested primers of the 16S rRNA and the hippuricase (hip O) genes to detect Campylobacter jejuni and Campylobacter coli in environmental samples.

    abstract::Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bang DD,Wedderkopp A,Pedersen K,Madsen M

    更新日期:2002-10-01 00:00:00

  • Use of RAPD for the detection of genetic variation in the human blood fluke, Schistosoma japonicum, from mainland China.

    abstract::A random amplified polymorphic DNA (RAPD) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of Schistosoma japonicum from seven geographical locations (Sj1: Zhejiang; Sj2: Anhui; Sj3: Jiangxi; Sj4: Hunan; Sj5: Hubei; Sj6: Sichuan; Sj7: Yunnan) of the People's Republic of China. D...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Gasser RB,Bao-Zhen Q,Nansen P,Johansen MV,Bøgh H

    更新日期:1996-10-01 00:00:00

  • Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples.

    abstract::Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage in cell culture is performed. A total of 54 archived faecal samples were examined. The heteroge...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bolanaki E,Kottaridi C,Dedepsidis E,Kyriakopoulou Z,Pliaka V,Pratti A,Levidiotou-Stefanou S,Markoulatos P

    更新日期:2008-06-01 00:00:00

  • Potential of recombinant flagellin fragment from Burkholderia thailandensis as an antigen for melioidosis antibody detection by indirect ELISA.

    abstract::Non-pathogenic Burkholderia thailandensis may be used as a model for Burkholderia pseudomallei due to the genetic similarity of these species. Moreover, the experimental manipulation of B. thailandensis is safer. In this study, we constructed recombinant flagellin protein fragments of B. thailandensis E264 (FLAG300, F...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wajanarogana S,Nimnuch P,Thongmee A,Kritsiriwuthinan K

    更新日期:2013-04-01 00:00:00

  • Potential regulatory SNPs in promoters of human genes: a systematic approach.

    abstract::Single nucleotide polymorphisms (SNPs) can significantly contribute to the cellular level of the mRNA transcripts encoded by human disease related genes. DNA variations between individuals can be an indication of predisposition to disease or affect the response to treatment. An algorithm allowing in silico extraction ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Stepanova M,Tiazhelova T,Skoblov M,Baranova A

    更新日期:2006-12-01 00:00:00

  • Identification of six novel CFTR mutations in a sample of Italian cystic fibrosis patients.

    abstract::The spectrum of cystic fibrosis (CF) mutations has been determined in many populations of different ethnic and geographic origins. However, in the south of Europe, the commonest mutation, delta F508, accounts for only about 50% of CF chromosomes, while identification of most of the other mutant alleles has not been ac...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Férec C,Novelli G,Verlingue C,Quéré I,Dallapiccola B,Audrézet MP,Mercier B

    更新日期:1995-04-01 00:00:00

  • Expression patterns of miR-146a and miR-146b in mastitis infected dairy cattle.

    abstract::This study reports a significant up-regulation of bta-miR-146a and bta-miR-146b expression levels in bovine mammary tissues infected with subclinical, clinical and experimental mastitis. Potential target genes are involved in multiple immunological pathways. These results suggest a regulatory function of both miRNAs f...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wang XP,Luoreng ZM,Zan LS,Raza SH,Li F,Li N,Liu S

    更新日期:2016-10-01 00:00:00

  • Development and validation of a TTR-specific copy number screening tool, and application to potentially relevant patient cohorts.

    abstract::TTR amyloidosis (ATTR) is a fatal condition caused by extracellular deposits of misfolded transthyretin. Patients often present with cardiac disease, but manifestations may also involve other organs including the peripheral nervous system. ATTR is considered familial when heterozygous mutations in the TTR gene are pre...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Jahic A,Bock A,Duca F,Bonderman D,Mascherbauer J,Windhager R,Auer-Grumbach M,Beetz C

    更新日期:2018-10-01 00:00:00

  • Genomic structure of the EPHA1 receptor tyrosine kinase gene.

    abstract::Some receptor tyrosine kinase genes are mutated in inherited and somatically acquired human cancers. To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions. The gene contains 18 exons, two...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Owshalimpur D,Kelley MJ

    更新日期:1999-06-01 00:00:00

  • Multiple paternity and sperm storage in captive Hermann's tortoises, Testudo hermanni boettgeri determined from amniotic fluid adhering to the eggshell.

    abstract::We identified multiple paternity in 52.9% of the clutches of Hermann's tortoise Testudo hermanni boettgeri using polymorphic microsatellite markers. In addition we demonstrated sperm storage across seasons. DNA was extracted from the amniotic fluid adhering to the eggshell's inner surface, a procedure suitable for eas...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Farke CM,Olek K,Gerding WM,Distler C

    更新日期:2015-08-01 00:00:00

  • Molecular detection and identification of enteroviruses in children admitted to a university hospital in Greece.

    abstract::Although enteroviral infections occur frequently during childhood, the circulation of particular serotypes has never been studied in Greece. The objectives of the present report were molecular detection and identification of human enteroviruses in children admitted with nonspecific febrile illness or meningitis to a u...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Siafakas N,Attilakos A,Vourli S,Stefos E,Meletiadis J,Nikolaidou P,Zerva L

    更新日期:2011-10-01 00:00:00

  • A duplex PCR assay for the simultaneous detection and differentiation of Muscovy duck parvovirus and goose parvovirus.

    abstract::Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific prim...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wan C,Cheng L,Chen C,Liu R,Shi S,Fu G,Chen H,Fu Q,Huang Y

    更新日期:2019-10-01 00:00:00

  • Evaluation of PCR and nested PCR for laboratory diagnosis of hepatitis C virus infection.

    abstract::The detection of hepatitis C virus (HCV) RNA by nested polymerase chain reaction (PCR) is believed to be the most reliable method to diagnose HCV infections. A pitfall of nested PCR is that it is prone to contamination. Single step reverse transcription-PCR (RT-PCR) was performed, prospectively, on 80 sera from 59 pat...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Aslanzadeh J,Padilla BB,Shanley JD

    更新日期:1996-06-01 00:00:00

  • Development of a multiplex PCR assay for the identification of pathogenic genes of Escherichia coli in milk and milk products.

    abstract::A multiplex PCR for the simultaneous detection of some pathogenic genes of enteropathogenic, enterotoxigenic and verocytotoxin-producing Escherichia coli was developed. In this study primers found in literature as well as primers to the purpose designed were used. In this way, it was possible to generate specific frag...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bottero MT,Dalmasso A,Soglia D,Rosati S,Decastelli L,Civera T

    更新日期:2004-08-01 00:00:00