Human papillomavirus type 33 DNA and E6-E7 transcripts in late passages of the UT-DEC-1 vaginal keratinocyte cell line.

Abstract:

:Transcription of human papillomavirus (HPV) type 33 early region was analysed in the UT-DEC-1 keratinocyte cell line, which has been derived from a HPV-33-containing mild vaginal dysplasia. Fifteen cDNA clones from transcripts from the E6-E7 open reading frames were constructed and analysed. Most clones represented viral transcripts spliced within the E6 open reading frame, probably encoding the E7 protein. Interestingly, a less abundant unspliced transcript species with coding capacity for the full length E6 protein was found, reported here for the first time for the malignancy-associated HPV type 33.

journal_name

Mol Cell Probes

authors

Auvinen E,Hietanen S,Gissmann L,Alonso A

doi

10.1006/mcpr.1997.0144

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

59-62

issue

1

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(97)90144-4

journal_volume

12

pub_type

杂志文章
  • Use of DNA restriction endonuclease digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from human and animal hosts.

    abstract::Variation amongst strains of Helicobacter pylori and Helicobacter mustelae was examined by DNA restriction endonuclease digestion and rRNA gene patterns generated using a non-radioactive probe. Chromosomal DNA was extracted from 30 cultures of H. pylori from human, Rhesus monkey and pig gastric mucosa, and from three ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/0890-8508(90)90023-s

    authors: Morgan DD,Owen RJ

    更新日期:1990-08-01 00:00:00

  • A fluorescent method for detecting low-grade 11patUPD mosaicism in Beckwith-Wiedemann syndrome.

    abstract::The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2003.07.002

    authors: Russo S,Mencarelli M,Cavalleri F,Selicorni A,Cogliati F,Larizza L

    更新日期:2003-12-01 00:00:00

  • Detection and molecular typing of Campylobacter jejuni in fecal samples by polymerase chain reaction.

    abstract::In order to determine whether polymerase chain reaction (PCR) could be used to detect Campylobacter jejuni directly in stool samples, DNA from 66 frozen culture positive and negative fecal samples was purified by column chromatography. The flaA gene was amplified using primers directed against the conserved 5' and 3' ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1996.0011

    authors: Waegel A,Nachamkin I

    更新日期:1996-04-01 00:00:00

  • Rapid and visual detection of dengue virus using recombinase polymerase amplification method combined with lateral flow dipstick.

    abstract::Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2019.06.003

    authors: Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

    更新日期:2019-08-01 00:00:00

  • Polymorphism rs3737787 of Upstream Stimulatory Factor 1 gene is associated with serum lipid phenotype in Nigerian population.

    abstract::Serum lipid profile which is determined by genotype-phenotype relationship plays a significant role in the development of cardiovascular disease. Upstream stimulatory factor 1 (USF1), has been reported to be associated with serum lipid levels in different population, hence, this study investigated the association of v...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2020.101687

    authors: Onadeko OT,Okunowo WO,Imaga NOA,Abdulrazaq MM,Onuminya OJ,Van-Lare TO,Nwosu M

    更新日期:2020-12-08 00:00:00

  • Rapid and cost effective genotyping method for polymorphisms in PPARG, PPARGC1 and TCF7L2 genes.

    abstract::Polymorphisms (rs1801282, rs8192678, rs7903146) of peroxisome proliferator-activated receptor gamma (PPARG), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PPARGC1A) and transcription factor 7-like 2 (TCF7L2) have recently been associated with different diseases, mainly type 2 diabetes. An assay...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2008.10.001

    authors: Habalová V,Klimcáková L,Zidzik J,Tkác I

    更新日期:2009-02-01 00:00:00

  • Evaluation of PCR and nested PCR for laboratory diagnosis of hepatitis C virus infection.

    abstract::The detection of hepatitis C virus (HCV) RNA by nested polymerase chain reaction (PCR) is believed to be the most reliable method to diagnose HCV infections. A pitfall of nested PCR is that it is prone to contamination. Single step reverse transcription-PCR (RT-PCR) was performed, prospectively, on 80 sera from 59 pat...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1996.0024

    authors: Aslanzadeh J,Padilla BB,Shanley JD

    更新日期:1996-06-01 00:00:00

  • Screening for cystic fibrosis in dried blood spots of newborns.

    abstract::We propose a newborn cystic fibrosis (CF) screening test based on the analysis of dried blood spot DNA by a strategy involving simple or multiplex denaturing gradient gel electrophoresis (DGGE) of PCR products of CFTR gene fragments, in conjunction with the immunoreactive-trypsin (IRT) assay. From May 1988 to May 1992...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1993.1073

    authors: Audrézet MP,Costes B,Ghanem N,Fanen P,Verlingue C,Morin JF,Mercier B,Goossens M,Férec C

    更新日期:1993-12-01 00:00:00

  • Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis.

    abstract::Beak atrophy and dwarfism syndrome (BADS) is commonly caused by co-infection with duck circovirus (DuCV) and novel goose parvovirus (NGPV). Therefore, concurrent detection of both viruses is important for monitoring and limiting BADS, although such a diagnostic test has not been reported. In this study, we developed a...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2020.101648

    authors: Wang Y,Cui Y,Li Y,Jiang S,Liu H,Wang J,Li Y

    更新日期:2020-10-01 00:00:00

  • A novel RT-PCR approach for detection and characterization of citrus viroids.

    abstract::Citrus plants are natural hosts of five viroid species and a large number of sequence variants. Because of their small size, viroids lend themselves to various RT-PCR approaches for their detection and further characterization. The one-step RT-PCR approach proposed here is based on the synthesis of viroid-cDNA by reve...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2005.11.001

    authors: Bernad L,Duran-Vila N

    更新日期:2006-04-01 00:00:00

  • A species-specific DNA probe for Providencia stuartii identification.

    abstract::A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments perf...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/0890-8508(92)90036-w

    authors: Thaller MC,Berlutti F,Riccio ML,Rossolini GM

    更新日期:1992-10-01 00:00:00

  • Rapid PCR-based delineation of the porcine nodular worms, Oesophagostomum dentatum and O. quadrispinulatum.

    abstract::At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1997.0097

    authors: Newton LA,Chilton NB,Monti JR,Bjørn H,Várady M,Christensen CM,Gasser RB

    更新日期:1997-04-01 00:00:00

  • Rapid genomic typing of BK virus directly from clinical specimens.

    abstract::A simple and rapid method, PCR-restriction enzyme analysis (PCR-RE) for BK virus (BKV) typing was developed, based on the presence of type-specific restriction enzyme sites in a 327 bp PCR-generated fragment which partially encodes the VP1 protein. The enzymes, Alu I, Xmn I and Ava II, were used to digest the PCR prod...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1993.1047

    authors: Jin L

    更新日期:1993-08-01 00:00:00

  • Detection of adenovirus in the waters of the Seine River estuary by nested-PCR.

    abstract::Several systems for isolating viruses from environmental samples have been tested. The most promising method is based on genomic amplification. The authors attempted to detect adenovirus in nucleic-acid extracts from the Seine River estuary by a two-step amplification of a 220-bp segment of the conserved coding region...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1998.0166

    authors: Castignolles N,Petit F,Mendel I,Simon L,Cattolico L,Buffet-Janvresse C

    更新日期:1998-06-01 00:00:00

  • Multiplex PCR for avian pathogenic mycoplasmas.

    abstract::Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasm...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1997.0108

    authors: Wang H,Fadl AA,Khan MI

    更新日期:1997-06-01 00:00:00

  • DNA hybridization for diagnosis of pertussis.

    abstract::The aim of the present study was to evaluate a mixed phase DNA hybridization assay for detection of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal aspirates from patients with suspected pertussis. Among 179 consecutive patients with own or parental suspicion of pertussis, the diagnosis was confirm...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/0890-8508(90)90021-q

    authors: Reizenstein E,Morfeldt E,Granström G,Granström M,Löfdahl S

    更新日期:1990-08-01 00:00:00

  • Differentiation of three species of ixodid tick, Dermacentor andersoni, D. variabilis and D. albipictus, by PCR-based approaches using markers in ribosomal DNA.

    abstract::Three species of Dermacentor, Dermacentor albipictus, Dermacentor andersoni and Dermacentor variabilis, commonly occur in Canada. D. andersoni and D. variabilis are morphologically similar and are important vectors of human and animal pathogens. A practical polymerase chain reaction (PCR) assay, based on the amplifica...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2007.04.003

    authors: Dergousoff SJ,Chilton NB

    更新日期:2007-10-01 00:00:00

  • Successful quantification of cytomegalovirus DNA by competitive PCR and detection with capillary electrophoresis.

    abstract::Human cytomegalovirus (HCMV) is responsible for severe infections in immunocompromised patients. Viral load has recently been identified as one of the major risk factors for subsequent development of HCMV disease. In this context, we developed a protocol allowing rapid, sensitive and precise quantification of HCMV DNA...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1996.0071

    authors: Poirier-Toulemonde AS,Imbert-Marcille BM,Ferré-Aubineau V,Besse B,Le Roux MG,Cantarovich D,Billaudel S

    更新日期:1997-02-01 00:00:00

  • Specific detection of Campylobacter concisus by PCR amplification of 23S rDNA areas.

    abstract::The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. conc...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/s0890-8508(95)90114-0

    authors: Bastyns K,Chapelle S,Vandamme P,Goossens H,De Wachter R

    更新日期:1995-08-01 00:00:00

  • Acquisition of uncharacterized sequences from Candidatus liberibacter, an unculturable bacterium, using an improved genomic walking method.

    abstract::An effective PCR-based genomic walking approach is described to discover previously unknown flanking genomic DNA sequences from Candidatus Liberibacter asiaticus, an unculturable, phloem-limited bacterium. Using this technique, 8564bp of new DNA sequences were obtained from three genomic loci; tufB-secE-nusG-rplKAJL-r...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2007.06.006

    authors: Lin H,Doddapaneni H,Bai X,Yao J,Zhao X,Civerolo EL

    更新日期:2008-02-01 00:00:00

  • Hsa-miR-346 plays a role in the development of sepsis by downregulating SMAD3 expression and is negatively regulated by lncRNA MALAT1.

    abstract::Sepsis is a common complication in infection, trauma, and surgery. Severe sepsis has been identified as the leading cause of death in patients suffering from noncardiovascular ailments in intensive care units. In the current study, we used lipopolysaccharide (LPS) to stimulate the mouse macrophage cell line RAW264.7, ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2019.101444

    authors: Yang Q,Cao K,Jin G,Zhang J

    更新日期:2019-10-01 00:00:00

  • Real-time PCR using SYBR Green for the detection of Shigella spp. in food and stool samples.

    abstract::Shigella spp are exquisitely fastidious Gram negative organisms that frequently get missed in the detection by traditional culture methods. For this reason, this work has adapted a classical PCR for detection of Shigella in food and stool specimens to real-time PCR using the SYBR Green format. This method follows a me...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2012.09.002

    authors: Mokhtari W,Nsaibia S,Gharbi A,Aouni M

    更新日期:2013-02-01 00:00:00

  • Endostar regulates EMT, migration and invasion of lung cancer cells through the HGF-Met pathway.

    abstract:AIM:Though Endostar (ES) could inhibit tumor growth by inhibiting tumor angiogenesis, other possible mechanisms have been less reported. This study aims to investigate the role of ES in the treatment of lung cancer from the perspective of macrophage-mediated epithelial mesenchymal transformation (EMT). METHODS:THP1 ce...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2019.05.003

    authors: Shen Y,Chen Q,Li L

    更新日期:2019-06-01 00:00:00

  • Impact of fixation artifacts and threshold selection on high resolution melting analysis for KRAS mutation screening.

    abstract:INTRODUCTION:Treatment in metastatic colorectal cancer (mCRC) has expanded with monoclonal antibodies targeting epidermal growth factor receptor, but is restricted to patients with a wild-type (WT) KRAS mutational status. The most sensitive assays for KRAS mutation detection in formalin-fixed paraffin embedded (FFPE) t...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2017.06.003

    authors: Pérez-Báez W,García-Latorre EA,Maldonado-Martínez HA,Coronado-Martínez I,Flores-García L,Taja-Chayeb L

    更新日期:2017-10-01 00:00:00

  • Direct detection of Actinobacillus pleuropneumoniae in swine lungs and tonsils by real-time recombinase polymerase amplification assay.

    abstract::Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was develop...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2019.03.007

    authors: Li R,Wang J,Liu L,Zhang R,Hao X,Han Q,Wang J,Yuan W

    更新日期:2019-06-01 00:00:00

  • Development of a realtime RT-PCR assay for the rapid detection of influenza A(H2) viruses.

    abstract::Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 196...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2017.06.005

    authors: Komissarov A,Fadeev A,Kosheleva A,Sintsova K,Grudinin M

    更新日期:2017-10-01 00:00:00

  • Competitive reverse transcription/polymerase chain reaction for the quantification of p53 and mdm2 mRNA expression.

    abstract::Wild-type p53 (wtp53) is a tumour suppressor gene involved in cell cycle regulation. The mdm2 protein can complex with the p53 protein and influence its function as a regulator of cell growth. To detect and quantify wtp53 and mdm2 mRNA expression, we established the competitive reverse transcription/polymerase chain r...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1006/mcpr.1996.0059

    authors: Totzke G,Sachinidis A,Vetter H,Ko Y

    更新日期:1996-12-01 00:00:00

  • Development and validation of a cost-effective in-house method, tetra-primer ARMS PCR assay, in genotyping of seven clinically important point mutations.

    abstract::The single nucleotide polymorphism (SNP) genotyping is currently considered as a particularly valuable tool for the diagnosis of different pathologies. For this reason, over the past several years a great deal of effort has been devoted to developing accurate, rapid, and cost-effective technologies for SNP analysis. A...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2011.04.005

    authors: Etlik O,Koksal V,Arican-Baris ST,Baris I

    更新日期:2011-08-01 00:00:00

  • Expression patterns of miR-146a and miR-146b in mastitis infected dairy cattle.

    abstract::This study reports a significant up-regulation of bta-miR-146a and bta-miR-146b expression levels in bovine mammary tissues infected with subclinical, clinical and experimental mastitis. Potential target genes are involved in multiple immunological pathways. These results suggest a regulatory function of both miRNAs f...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2016.08.004

    authors: Wang XP,Luoreng ZM,Zan LS,Raza SH,Li F,Li N,Liu S

    更新日期:2016-10-01 00:00:00

  • Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus.

    abstract::Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA L...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章

    doi:10.1016/j.mcp.2017.03.005

    authors: Yang Y,Qin X,Zhang W,Li Z,Zhang S,Li Y,Zhang Z

    更新日期:2017-06-01 00:00:00