Detection of adenovirus in the waters of the Seine River estuary by nested-PCR.

abstract：:We propose a newborn cystic fibrosis (CF) screening test based on the analysis of dried blood spot DNA by a strategy involving simple or multiplex denaturing gradient gel electrophoresis (DGGE) of PCR products of CFTR gene fragments, in conjunction with the immunoreactive-trypsin (IRT) assay. From May 1988 to May 1992...

journal_title：Molecular and cellular probes

authors： Audrézet MP,Costes B,Ghanem N,Fanen P,Verlingue C,Morin JF,Mercier B,Goossens M,Férec C

更新日期：1993-12-01 00:00:00

abstract：:A multiplex PCR was developed that is capable of detecting four of the most important bacterial agents of atypical pneumonia, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in uncultured patient specimens. These organisms cause similar symptomologies and are often not...

journal_title：Molecular and cellular probes

authors： McDonough EA,Barrozo CP,Russell KL,Metzgar D

更新日期：2005-10-01 00:00:00

abstract：:The hydroxymethylbilane synthase (HMBS) mRNAs from 44 control individuals and 30 patients suffering from acute intermittent porphyria (AIP), were screened for length differences by reverse transcriptase polymerase chain reaction (RT-PCR) and any abnormalities were characterized by direct sequencing. Examination of the...

journal_title：Molecular and cellular probes

authors： Ong PM,Lanyon WG,Moore MR,Connor JM

更新日期：1998-04-01 00:00:00

abstract：:The major outer-membrane protein (MOMP) from Campylobacter jejuni NCTC 11168 was purified by solubilization in Triton X-100. Whole-cell proteins of Campylobacter species and the MOMP were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linke...

journal_title：Molecular and cellular probes

authors： Taylor DE,Chang N

更新日期：1987-09-01 00:00:00

abstract：:In the present study, multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for simultaneously detection of six major shrimp viruses including yellow-head virus (YHV), white spot syndrome virus (WSSV), Taura syndrome virus (TSV), hepatopancreatic parvovirus (HPV), infectious hypodermal and ...

journal_title：Molecular and cellular probes

authors： Khawsak P,Deesukon W,Chaivisuthangkura P,Sukhumsirichart W

更新日期：2008-06-01 00:00:00

abstract：:Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

journal_title：Molecular and cellular probes

authors： Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

更新日期：2019-08-01 00:00:00

abstract：:Inexpensive fiberglass mesh window screens were used as spacers between colony blot filters to increase the number of bacterial isolates that could be tested by DNA colony hybridization. Sixty filters with up to 48 isolates per filter were tested at one time. This modified technique reduced the amount of radiolabelled...

journal_title：Molecular and cellular probes

authors： Kaysner CA,Weagant SD,Hill WE

更新日期：1988-12-01 00:00:00

abstract：:The single nucleotide polymorphism (SNP) genotyping is currently considered as a particularly valuable tool for the diagnosis of different pathologies. For this reason, over the past several years a great deal of effort has been devoted to developing accurate, rapid, and cost-effective technologies for SNP analysis. A...

journal_title：Molecular and cellular probes

authors： Etlik O,Koksal V,Arican-Baris ST,Baris I

更新日期：2011-08-01 00:00:00

abstract：:Quantum dot (QD) molecular beacons were explored for sequence-specific DNA detection. The effectiveness of multiple linkage strategies and fluorescence quenchers were compared in hybridization-based assays. To compare linkage strategies, covalent amide linkage and streptavidin-biotin binding were used to link semicond...

journal_title：Molecular and cellular probes

authors： Cady NC,Strickland AD,Batt CA

更新日期：2007-04-01 00:00:00

abstract：:An alkaline phosphatase (AP)-labeled genus-specific oligonucleotide probe was developed to detect and enumerate vibrios in shrimp larvae and their surrounding environment. The probe was evaluated using 35 laboratory isolates of Vibrio species and 29 isolates of non-vibrio species. The probe was specific for the Vibrio...

journal_title：Molecular and cellular probes

authors： Raghunath P,Karunasagar I,Karunasagar I

更新日期：2007-08-01 00:00:00

abstract：:A DNA amplification procedure using heat stable Taq polymerase and the polymerase chain reaction is described for the detection of Pseudomonas aeruginosa in specimens from cystic fibrosis patients. A set of primers was selected on the basis of the nucleotide sequence of the algD gene encoding GDP mannose dehydrogenase...

journal_title：Molecular and cellular probes

authors： McIntosh I,Govan JR,Brock DJ

更新日期：1992-08-01 00:00:00

abstract：:Shigella spp are exquisitely fastidious Gram negative organisms that frequently get missed in the detection by traditional culture methods. For this reason, this work has adapted a classical PCR for detection of Shigella in food and stool specimens to real-time PCR using the SYBR Green format. This method follows a me...

journal_title：Molecular and cellular probes

authors： Mokhtari W,Nsaibia S,Gharbi A,Aouni M

更新日期：2013-02-01 00:00:00

abstract：:A polymorphic GT dinucleotide repeat sequence has been identified in the 5' flanking region of the human growth hormone receptor (hGHR) gene on chromosome 5p13.1-p12, within the promoter region of the V9 5'UTR exon. Thirteen alleles have been identified in 50 non-related individuals, with an observed heterozygosity of...

journal_title：Molecular and cellular probes

authors： Hadjiyannakis S,Zheng H,Hendy GN,Goodyer CG

更新日期：2001-08-01 00:00:00

abstract：:The complete nucleotide sequence of 85A antigen of Mycobacterium gordonae was determined. This gene encodes 339 amino acids, including 43 amino acids for the signal peptide, followed by a mature protein of 296 amino acids. A polymerase chain reaction (PCR) assay for the rapid detection of M. gordonae DNA using two pai...

journal_title：Molecular and cellular probes

authors： Dumonceaux M,Fauville Dufaux M,Ooms J,De Wit L,Sonck P,Content J

更新日期：1997-08-01 00:00:00

abstract：:Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three reactions, through SYBR green I melting curve analysis. The method utilizes type-specific primer se...

journal_title：Molecular and cellular probes

authors： Tsakogiannis D,Papacharalampous M,Toska E,Kyriakopoulou Z,Dimitriou TG,Ruether IG,Komiotis D,Markoulatos P

更新日期：2015-02-01 00:00:00

abstract：:Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a...

journal_title：Molecular and cellular probes

authors： French DJ,Archard CL,Brown T,McDowell DG

更新日期：2001-12-01 00:00:00

abstract：:Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. We developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine. Consider...

journal_title：Molecular and cellular probes

authors： Mitui M,Leos NK,Lacey D,Doern C,Rogers BB,Park JY

更新日期：2013-10-01 00:00:00

abstract：:Bacterial kidney disease in salmonid fish is caused by the slow-growing Gram-positive rod, Renibacterium salmoninarum. The partial sequence of 16S rRNA from R. salmoninarum was determined and compared with published bacterial 16S rRNA sequences. From this sequence information, a 30-bases-long oligonucleotide was desig...

journal_title：Molecular and cellular probes

authors： Mattsson JG,Gersdorf H,Jansson E,Hongslo T,Göbel UB,Johansson KE

更新日期：1993-02-01 00:00:00

abstract：:One pair of high-sensitive polymerase chain reaction (PCR) primers for Cryptosporidium parvum was constructed based on the sequence of random amplified polymorphic DNA. PCR with this primer pair amplified only the DNA of C. parvum, not the control DNA including Cryptosporidium muris. This primer pair had most advantag...

journal_title：Molecular and cellular probes

authors： Wu Z,Nagano I,Matsuo A,Uga S,Kimata I,Iseki M,Takahashi Y

更新日期：2000-02-01 00:00:00

abstract：:The presence of human cytomegalovirus (HCMV) was tested in 388 cervicovaginal cells specimens obtained from the same number of pregnant women. HCMV was detected in 5.41%, 11.6% and 13.9% of these specimens by conventional culture, in situ DNA hybridization and polymerase chain reaction (PCR) methods, respectively. The...

journal_title：Molecular and cellular probes

authors： Yuan CF,Kao SM,Wang DC,Ng HT,Pao CC

更新日期：1990-12-01 00:00:00

abstract：:Wild-type p53 (wtp53) is a tumour suppressor gene involved in cell cycle regulation. The mdm2 protein can complex with the p53 protein and influence its function as a regulator of cell growth. To detect and quantify wtp53 and mdm2 mRNA expression, we established the competitive reverse transcription/polymerase chain r...

journal_title：Molecular and cellular probes

authors： Totzke G,Sachinidis A,Vetter H,Ko Y

更新日期：1996-12-01 00:00:00

abstract：:The impact that next-generation sequencing technology (NGS) is having on many aspects of molecular and cell biology, is becoming increasingly apparent. One of the most noticeable outcomes of the new technology in human genetics, has been the accelerated rate of identification of disease-causing genes. Especially for r...

journal_title：Molecular and cellular probes

authors： Morris-Rosendahl DJ,Kaindl AM

更新日期：2015-10-01 00:00:00

abstract：:DNA probes were used to examine tetracycline-resistant Gram-negative bacteria (281 strains representing eight species) from catfish ponds. The isolates, which did not previously hybridize with the Tet A, B and C determinants, were examined for the presence of tetracycline-resistance Tet D and Tet E determinants. The d...

journal_title：Molecular and cellular probes

authors： DePaola A,Roberts MC

更新日期：1995-10-01 00:00:00

abstract：:Multiplex ligation dependent probe amplification (MLPA) assays were designed for the genes HEXB (OMIM: 606873), GM2A (OMIM: 613109) and SMARCAL1 (OMIM: 606622) of humans. Two sets of synthetic MLPA probes for these coding exons were tested. Changes in copy numbers were detected as well as single nucleotide polymorphis...

journal_title：Molecular and cellular probes

authors： Sobek AK,Evers C,Dekomien G

更新日期：2013-02-01 00:00:00

abstract：:Development of rapid amplification assays for the detection and identification of biological threat agents has become a focus of diagnostic efforts in recent years. The use of real-time PCR assays as diagnostic tools depends upon two critical processes. First, nucleic acid purification must provide template that is bo...

journal_title：Molecular and cellular probes

authors： Hartman LJ,Coyne SR,Norwood DA

更新日期：2005-02-01 00:00:00

abstract：:We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant d...

journal_title：Molecular and cellular probes

authors： Hong SH,Song J,Kim JQ

更新日期：2001-04-01 00:00:00

abstract：:Homogenous fluorescence PCR assays offer distinct advantages for qualitative testing and are gaining immense popularity in fields like diagnostic microbiology. To meet the demand of high-volume laboratories, we developed a protocol for qualitative multiplex 5' nuclease assays using post-only PCR analysis. This novel a...

journal_title：Molecular and cellular probes

authors： Antonishyn NA,McDonald RR,Chan EL,Horsman GB

更新日期：2005-10-01 00:00:00

abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-rea...

journal_title：Molecular and cellular probes

authors： Chen HT,Zhang J,Ma YP,Ma LN,Ding YZ,Liu XT,Cai XP,Ma LQ,Zhang YG,Liu YS

更新日期：2010-04-01 00:00:00

abstract：:A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis. ...

journal_title：Molecular and cellular probes

authors： Ren H,Yang M,Zhang G,Liu S,Wang X,Ke Y,Du X,Wang Z,Huang L,Liu C,Chen Z

更新日期：2016-04-01 00:00:00

abstract：:alpha-SNAP is an essential component of the protein machinery responsible for membrane fusion events in different cell types. The hyh (hydrocephalus with hop gait) mouse carries a missense mutation in Napa gene that results in a point mutation (M105I) in alpha-SNAP protein. Homozygous animals for the mutant allele hav...

journal_title：Molecular and cellular probes

authors： Bátiz LF,Roales-Buján R,Rodríguez-Pérez LM,Matas IM,Páez P,Roque M,Jiménez AJ,Ramos C,Pérez-Fígares JM

更新日期：2009-12-01 00:00:00