DNA hybridization for diagnosis of pertussis.

Abstract:

:The aim of the present study was to evaluate a mixed phase DNA hybridization assay for detection of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal aspirates from patients with suspected pertussis. Among 179 consecutive patients with own or parental suspicion of pertussis, the diagnosis was confirmed in 103 patients by serology and in 52/103 (50%) cases also by culture. The remaining 76 patients served as nonpertussis controls. Direct hybridization was positive in 38% samples with serology as reference method, a non-significant difference to the 50% sensitivity for culture. Preculture of samples on membranes for 24, 48 and 72 h gave a significantly higher sensitivity only with 72 h preculture, 69% vs 50% (P = 0.007). The 72h preculture gave, however, also a significant decrease of specificity, 87% vs 100% for routine culture (P = 0.001) and is not a more rapid diagnostic method. The result shows that rapid diagnosis by DNA hybridization can be achieved in a large proportion of pertussis cases. The presence of smaller numbers of bacteria in samples only positive after preculture indicate that DNA hybridization could be a highly sensitive diagnostic method with further development of more rapid amplification systems.

journal_name

Mol Cell Probes

authors

Reizenstein E,Morfeldt E,Granström G,Granström M,Löfdahl S

doi

10.1016/0890-8508(90)90021-q

subject

Has Abstract

pub_date

1990-08-01 00:00:00

pages

299-306

issue

4

eissn

0890-8508

issn

1096-1194

pii

0890-8508(90)90021-Q

journal_volume

4

pub_type

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