A species-specific DNA probe for Providencia stuartii identification.

Abstract:

:A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.

journal_name

Mol Cell Probes

authors

Thaller MC,Berlutti F,Riccio ML,Rossolini GM

doi

10.1016/0890-8508(92)90036-w

subject

Has Abstract

pub_date

1992-10-01 00:00:00

pages

417-22

issue

5

eissn

0890-8508

issn

1096-1194

pii

0890-8508(92)90036-W

journal_volume

6

pub_type

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