Abstract:
:Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a new homogeneous method for fluorescence-based sequence detection, allele discrimination and DNA quantification. Hybridization of HyBeacons to complementary DNA target sequences results in a measurable elevation of probe fluorescence emission. HyBeacon probes may be incorporated into real-time polymerase chain reaction (PCR) assays to detect the presence and monitor the accumulation of specific DNA sequences. Furthermore, closely related sequences differing by as little as a single nucleotide may be discriminated by measuring the melting temperatures (T(m)) of various probe/target duplexes and exploiting the differences in T(m) that exist between different duplexes. We demonstrate here that HyBeacon probes are efficient tools for rapid sequence analysis and that a single probe may be employed to reliably identify homozygous and heterozygous samples. Additional benefits exhibited by the HyBeacon technology derive from their simple mode of action, ease of design, relatively inexpensive synthesis and potential for multiplex analysis.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
French DJ,Archard CL,Brown T,McDowell DGdoi
10.1006/mcpr.2001.0384subject
Has Abstractpub_date
2001-12-01 00:00:00pages
363-74issue
6eissn
0890-8508issn
1096-1194pii
S0890850801903846journal_volume
15pub_type
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2008.09.003
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1006/mcpr.2002.0408
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pub_type: 杂志文章
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2007.03.003
更新日期:2007-08-01 00:00:00
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2016.02.003
更新日期:2016-04-01 00:00:00
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1006/mcpr.1994.1051
更新日期:1994-10-01 00:00:00
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1006/mcpr.1993.1054
更新日期:1993-10-01 00:00:00
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2005.12.006
更新日期:2006-06-01 00:00:00
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2007.06.010
更新日期:2008-02-01 00:00:00
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2016.11.006
更新日期:2017-04-01 00:00:00
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1006/mcpr.1994.1017
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2019.101439
更新日期:2019-10-01 00:00:00
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2005.07.005
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
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