Abstract：
:Microsatellites have recently been used for linkage analysis of genetic diseases and for DNA fingerprinting in forensic medicine. In the present study the heterozygosity, PIC values and allele distributions of four microsatellites, D8S85, D8S88, D5S346 and D7S460, in an Italian population have been investigated. After amplification with primers specific for each locus, alleles were separated and detected by denaturing gel electrophoresis and ethidium bromide staining. High heterozygosity and PIC values were observed for all microsatellites in accordance with data in other Caucasian populations. However, different allele distributions for D8S85, D8S88 and D5S346, due to the presence of additional bands or to different frequencies, were found. D7S460, which has never been fully characterized before, appeared to have five alleles in the range 172 to 188 bp. When used for paternity testing, all microsatellites gave results which were consistent with those obtained with established markers, including apo B 3'HVR, D1S80 and COL2A1. This indicates that D8S85, D8S88, D5S346 and D7S460 may be useful as additional informative markers or for solving discrepancies in selected cases.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Sirchia SM,Garagiola I,De Andreis C,Gazzoli I,Gramegna M,Colucci Gdoi
10.1006/mcpr.1996.0021subject
Has Abstractpub_date
1996-04-01 00:00:00pages
155-8issue
2eissn
0890-8508issn
1096-1194pii
S0890-8508(96)90021-3journal_volume
10pub_type
杂志文章abstract：:Some receptor tyrosine kinase genes are mutated in inherited and somatically acquired human cancers. To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions. The gene contains 18 exons, two...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1999.0228
更新日期：1999-06-01 00:00:00
abstract：:The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2003.07.002
更新日期：2003-12-01 00:00:00
abstract：:An example of the application and contruction of a polymerase chain reaction (PCR) internal control is presented. The internal control is synthesized in one PCR reaction. The primers used in this reaction possess 5' over-hanging ends which are identical to the primers used in the diagnostic reaction, whereas their 3' ...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1998.0170
更新日期：1998-10-01 00:00:00
abstract：:The polymerase spiral reaction (PSR), a novel isothermal method for targeted DNA amplification, was effectively applied to detect Salmonella in artificially spiked pork. The specificity of the developed PSR was tested using 16 Salmonella and 15 non-Salmonella strains. The PSR assay was 10-fold more sensitive than conv...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2020.101510
更新日期：2020-04-01 00:00:00
abstract：:We have used the polymerase chain reaction (PCR) to detect shigellae, EIEC and ETEC in stool specimens of diarrhoeic patients returning from tropical countries. As compared to culture (7.1% positive specimens), which recognizes only Shigella strains, PCR performed on bacterial growth from directly inoculated MacConkey...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1994.1040
更新日期：1994-08-01 00:00:00
abstract：OBJECT:To investigate the role of circHIPK3 in melanoma. METHODS:Bioinformatics analysis and experiments including RT-qPCR, Pearson's correlation analysis, luciferase reporter, Western blot, and RIP assays were applied to explore the function and mechanism of circHIPK3 in melanoma. RESULTS:CircHIPK3 expression was st...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2020.101644
更新日期：2020-10-01 00:00:00
abstract：:DNA aptamers (PSA-H and MT32) were applied for the detection of Apple stem pitting virus (ASPV) isolates using an Enzyme-Linked Oligonucleotide Assay (ELONA) and Western blot analysis. The specificity and effectiveness of aptamers were verified in comparison to a conventional Enzyme Linked Immunosorbent Assay (ELISA)....
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2017.10.001
更新日期：2017-12-01 00:00:00
abstract：:We have developed a new methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase (TdT) using a thioflavin T probe. This method is highly selective and sensitive. The fluorescence intensity was direct proportion to Dam MTase concentration in...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2016.02.001
更新日期：2016-04-01 00:00:00
abstract：AIM:Though Endostar (ES) could inhibit tumor growth by inhibiting tumor angiogenesis, other possible mechanisms have been less reported. This study aims to investigate the role of ES in the treatment of lung cancer from the perspective of macrophage-mediated epithelial mesenchymal transformation (EMT). METHODS:THP1 ce...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2019.05.003
更新日期：2019-06-01 00:00:00
abstract：:An alkaline phosphatase (AP)-labeled genus-specific oligonucleotide probe was developed to detect and enumerate vibrios in shrimp larvae and their surrounding environment. The probe was evaluated using 35 laboratory isolates of Vibrio species and 29 isolates of non-vibrio species. The probe was specific for the Vibrio...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2007.03.003
更新日期：2007-08-01 00:00:00
abstract：:As part of the development of the ligase chain reaction (LCR) into a tool which can be used by a wide variety of researchers, we have investigated several analytical detection systems for the products of this amplification reaction. While early work with this technology has used gel electrophoresis to separate the LCR...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1993.1027
更新日期：1993-06-01 00:00:00
abstract：:Serum lipid profile which is determined by genotype-phenotype relationship plays a significant role in the development of cardiovascular disease. Upstream stimulatory factor 1 (USF1), has been reported to be associated with serum lipid levels in different population, hence, this study investigated the association of v...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2020.101687
更新日期：2020-12-08 00:00:00
abstract：:Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1994.1035
更新日期：1994-06-01 00:00:00
abstract：:We identified multiple paternity in 52.9% of the clutches of Hermann's tortoise Testudo hermanni boettgeri using polymorphic microsatellite markers. In addition we demonstrated sperm storage across seasons. DNA was extracted from the amniotic fluid adhering to the eggshell's inner surface, a procedure suitable for eas...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2015.05.009
更新日期：2015-08-01 00:00:00
abstract：:This study reports a significant up-regulation of bta-miR-146a and bta-miR-146b expression levels in bovine mammary tissues infected with subclinical, clinical and experimental mastitis. Potential target genes are involved in multiple immunological pathways. These results suggest a regulatory function of both miRNAs f...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2016.08.004
更新日期：2016-10-01 00:00:00
abstract：:This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2020.101612
更新日期：2020-10-01 00:00:00
abstract：:The detection of hepatitis C virus (HCV) RNA by nested polymerase chain reaction (PCR) is believed to be the most reliable method to diagnose HCV infections. A pitfall of nested PCR is that it is prone to contamination. Single step reverse transcription-PCR (RT-PCR) was performed, prospectively, on 80 sera from 59 pat...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1996.0024
更新日期：1996-06-01 00:00:00
abstract：:The G-->A transition at nucleotide 21881 of the human catechol-O-methyltransferase (COMT) gene represents a functional genetic polymorphism (Val158Met), rendering an enzyme with reduced activity that has been associated with psychiatric disorders and estrogen-related cancers. A new method for the detection of this pol...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2006.12.001
更新日期：2007-06-01 00:00:00
abstract：:The pyrazinamidase gene coding for the enzyme that activates the bactericidal drug pyrazinamide contains a polymorphic site that is preserved in Mycobacterium bovis. We synthesized two sets of primers, one encompassing a 180 bp fragment, and the second spanning a 726 bp fragment including the full pncA gene. Following...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2003.11.006
更新日期：2004-06-01 00:00:00
abstract：:Variable number tandem repeat (VNTR) is a frequently employed typing method of Mycobacterium avium paratuberculosis (MAP) isolates. Based on whole genome sequencing in a previous study, allelic diversity at some VNTR loci seems to over- or under-estimate the actual phylogenetic variance among isolates. Interestingly, ...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2016.02.002
更新日期：2016-06-01 00:00:00
abstract：:We have developed and evaluated an ELISA-based detection method for PCR-amplified HIV-1 DNA. The assay uses two oligonucleotide probes which are end-labelled at the 5'-end with biotin or digoxigenin, respectively. Upon solution hybridization of these probes which react with the same strand of amplified DNA product, th...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1993.1054
更新日期：1993-10-01 00:00:00
abstract：:Holstein haplotype (HH) 1, 3 and 4 are lethal mutations, responsible for early embryonic losses in Holstein Friesian (HF) cattle, worldwide. Three PCR based assays - tetra Amplification Refractory Mutation System PCR, PCR primer induced restriction analysis and PCR-restriction fragment length polymorphism techniques f...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/j.mcp.2019.101503
更新日期：2020-04-01 00:00:00
abstract：:Six stable hybridoma cell lines secreting monoclonal antibodies to human calpastatin were established. All monoclonal antibodies belong to the IgG1 subclass and recognized different epitopes on calpastatin. At least two groups were distinguished; the first group was specific for muscle-type (M-) calpastatin and the se...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/0890-8508(91)90047-n
更新日期：1991-08-01 00:00:00
abstract：:Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.2001.0384
更新日期：2001-12-01 00:00:00
abstract：:The aim of the present study was to evaluate a mixed phase DNA hybridization assay for detection of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal aspirates from patients with suspected pertussis. Among 179 consecutive patients with own or parental suspicion of pertussis, the diagnosis was confirm...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/0890-8508(90)90021-q
更新日期：1990-08-01 00:00:00
abstract：:The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. conc...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/s0890-8508(95)90114-0
更新日期：1995-08-01 00:00:00
abstract：:Arcobacter is a recently described species, previously considered part of the Campylobacter family. A sensitive assay such as that provided by PCR could help to distinguish the closely related Arcobacter from Campylobacter. A PCR method to specifically detect both Campylobacter jejuni and Arcobacter butzleri in the sa...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.2000.0290
更新日期：2000-04-01 00:00:00
abstract：:Species identification of Mycobacterium tuberculosis remains a cumbersome process. We have developed a simple method for treating clinical samples which permits direct polymerase chain reaction (PCR) amplification of mycobacterial target DNA without organic extraction. Samples were boiled for 30 min in TE-Triton, then...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/s0890-8508(06)80011-3
更新日期：1991-10-01 00:00:00
abstract：:The ability to rapidly and reliably genotype mice is an important concern. Traditional methods employ labour intensive and time consuming techniques such as test crossing, gel electrophoresis or nucleic acid hybridization. Here we show that a new molecular biology workstation, the WAVE DNA Fragment Analysis System, ca...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1006/mcpr.1999.0239
更新日期：1999-06-01 00:00:00
abstract：:DNA probes were used to examine tetracycline-resistant Gram-negative bacteria (281 strains representing eight species) from catfish ponds. The isolates, which did not previously hybridize with the Tet A, B and C determinants, were examined for the presence of tetracycline-resistance Tet D and Tet E determinants. The d...
journal_title：Molecular and cellular probes
pub_type： 杂志文章
doi：10.1016/s0890-8508(95)91572-9
更新日期：1995-10-01 00:00:00
