A pncA polymorphism to differentiate between Mycobacterium bovis and Mycobacterium tuberculosis.

Abstract:

:The pyrazinamidase gene coding for the enzyme that activates the bactericidal drug pyrazinamide contains a polymorphic site that is preserved in Mycobacterium bovis. We synthesized two sets of primers, one encompassing a 180 bp fragment, and the second spanning a 726 bp fragment including the full pncA gene. Following PCR of Mycobacterium tuberculosis and M. bovis samples, it is possible to discriminate by this polymorphism between these species by digestion with Eco065 I. Digestion of the 180 bp fragment results in two fragments of 101 and 79 bp, specific for M. tuberculosis. Alternatively, digestion of the 726 bp fragment yields three fragments of 452, 165 and 109 bp for M. tuberculosis, but only two fragments of 561 and 165 bp for M. bovis.

journal_name

Mol Cell Probes

authors

Barouni AS,Augusto CJ,Lopes MT,Zanini MS,Salas CE

doi

10.1016/j.mcp.2003.11.006

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

167-70

issue

3

eissn

0890-8508

issn

1096-1194

pii

S089085080300118X

journal_volume

18

pub_type

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