Abstract:
:The pyrazinamidase gene coding for the enzyme that activates the bactericidal drug pyrazinamide contains a polymorphic site that is preserved in Mycobacterium bovis. We synthesized two sets of primers, one encompassing a 180 bp fragment, and the second spanning a 726 bp fragment including the full pncA gene. Following PCR of Mycobacterium tuberculosis and M. bovis samples, it is possible to discriminate by this polymorphism between these species by digestion with Eco065 I. Digestion of the 180 bp fragment results in two fragments of 101 and 79 bp, specific for M. tuberculosis. Alternatively, digestion of the 726 bp fragment yields three fragments of 452, 165 and 109 bp for M. tuberculosis, but only two fragments of 561 and 165 bp for M. bovis.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Barouni AS,Augusto CJ,Lopes MT,Zanini MS,Salas CEdoi
10.1016/j.mcp.2003.11.006subject
Has Abstractpub_date
2004-06-01 00:00:00pages
167-70issue
3eissn
0890-8508issn
1096-1194pii
S089085080300118Xjournal_volume
18pub_type
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