Class D and E tetracycline resistance determinants in gram-negative bacteria from catfish ponds.

abstract：:Single nucleotide polymorphisms (SNPs) can significantly contribute to the cellular level of the mRNA transcripts encoded by human disease related genes. DNA variations between individuals can be an indication of predisposition to disease or affect the response to treatment. An algorithm allowing in silico extraction ...

journal_title：Molecular and cellular probes

authors： Stepanova M,Tiazhelova T,Skoblov M,Baranova A

更新日期：2006-12-01 00:00:00

abstract：:Idiopathic generalized epilepsy (IGE) comprises a heterogeneous group of disorders, in which a high genetic predisposition and a complex mode of inheritance have been suggested. However, genes, which confer liability to common IGE subtypes including juvenile myoclonic epilepsy (JME) and childhood absence epilepsy (CAE...

journal_title：Molecular and cellular probes

authors： Haug K,Kremerskothen J,Hallmann K,Sander T,Dullinger J,Rau B,Beyenburg S,Lentze MJ,Barnekow A,Elger CE,Propping P,Heils A

更新日期：2000-08-01 00:00:00

abstract：:Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 196...

journal_title：Molecular and cellular probes

authors： Komissarov A,Fadeev A,Kosheleva A,Sintsova K,Grudinin M

更新日期：2017-10-01 00:00:00

abstract：:Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA L...

journal_title：Molecular and cellular probes

authors： Yang Y,Qin X,Zhang W,Li Z,Zhang S,Li Y,Zhang Z

更新日期：2017-06-01 00:00:00

abstract：:One pair of high-sensitive polymerase chain reaction (PCR) primers for Cryptosporidium parvum was constructed based on the sequence of random amplified polymorphic DNA. PCR with this primer pair amplified only the DNA of C. parvum, not the control DNA including Cryptosporidium muris. This primer pair had most advantag...

journal_title：Molecular and cellular probes

authors： Wu Z,Nagano I,Matsuo A,Uga S,Kimata I,Iseki M,Takahashi Y

更新日期：2000-02-01 00:00:00

abstract：:Although enteroviral infections occur frequently during childhood, the circulation of particular serotypes has never been studied in Greece. The objectives of the present report were molecular detection and identification of human enteroviruses in children admitted with nonspecific febrile illness or meningitis to a u...

journal_title：Molecular and cellular probes

authors： Siafakas N,Attilakos A,Vourli S,Stefos E,Meletiadis J,Nikolaidou P,Zerva L

更新日期：2011-10-01 00:00:00

abstract：:Hypertriglyceridemia (HTG) plays an important role in the development and progression of atherosclerosis. It is inherited in an autosomal dominant pattern with a frequency of approximately 1:1,000,000 worldwide. Previous study has demonstrated that more than six genes underlie this disorder. In addition, copy number v...

journal_title：Molecular and cellular probes

authors： Li JJ,Chen YQ,Fan LL,Jin JY,Guo S,Xiang R

更新日期：2018-02-01 00:00:00

abstract：:We have developed and evaluated an ELISA-based detection method for PCR-amplified HIV-1 DNA. The assay uses two oligonucleotide probes which are end-labelled at the 5'-end with biotin or digoxigenin, respectively. Upon solution hybridization of these probes which react with the same strand of amplified DNA product, th...

journal_title：Molecular and cellular probes

authors： Böni J,Schüpbach J

更新日期：1993-10-01 00:00:00

abstract：:We have developed a highly specific and sensitive technique for the detection of Pneumocystis carinii DNA using DNA amplification by the polymerase chain reaction (PCR). PCR products are detected by agarose gel electrophoresis and Southern hybridization to an oligonucleotide probe. Here we report the calibration of pa...

journal_title：Molecular and cellular probes

authors： Peters SE,Wakefield AE,Banerji S,Hopkin JM

更新日期：1992-04-01 00:00:00

abstract：:As part of the development of the ligase chain reaction (LCR) into a tool which can be used by a wide variety of researchers, we have investigated several analytical detection systems for the products of this amplification reaction. While early work with this technology has used gel electrophoresis to separate the LCR...

journal_title：Molecular and cellular probes

authors： Winn-Deen ES,Batt CA,Wiedmann M

更新日期：1993-06-01 00:00:00

abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-rea...

journal_title：Molecular and cellular probes

authors： Chen HT,Zhang J,Ma YP,Ma LN,Ding YZ,Liu XT,Cai XP,Ma LQ,Zhang YG,Liu YS

更新日期：2010-04-01 00:00:00

abstract：:A polymorphic GT dinucleotide repeat sequence has been identified in the 5' flanking region of the human growth hormone receptor (hGHR) gene on chromosome 5p13.1-p12, within the promoter region of the V9 5'UTR exon. Thirteen alleles have been identified in 50 non-related individuals, with an observed heterozygosity of...

journal_title：Molecular and cellular probes

authors： Hadjiyannakis S,Zheng H,Hendy GN,Goodyer CG

更新日期：2001-08-01 00:00:00

abstract：:Protein tyrosine phosphatase, non-receptor type 14 (PTPN14) exerts a profound effect in the progression of multiple malignant tumors. However, whether PTPN14 plays a role in prostate cancer has not been well investigated. Herein, we evaluated the function and potential underlying mechanism of PTPN14 in prostate cancer...

journal_title：Molecular and cellular probes

authors： Wang R,Du Y,Shang J,Dang X,Niu G

更新日期：2020-10-01 00:00:00

abstract：:Non-small-cell lung carcinoma (NSCLC) accounts for approximately 80% of lung cancers with a high metastatic potential. Elucidating the mechanism of NSCLC metastasis will provide new promising targets for NSCLC therapy and benefit its prognosis. Plasmacytoma variant translocation 1 (PVT1) has been proven to be overexpr...

journal_title：Molecular and cellular probes

authors： Su XH,Zhu YR,Hou YJ,Li K,Dong NH

更新日期：2020-12-01 00:00:00

abstract：:The effects of comprehensive LNA substitution in PCR primers for amplification of human genomic DNA targets are presented in this report. Previous research with LNA in other applications has shown interesting properties for molecular hybridization including enhanced specificity in allele-specific PCR. Here we systemat...

journal_title：Molecular and cellular probes

authors： Latorra D,Arar K,Hurley JM

更新日期：2003-10-01 00:00:00

abstract：:A multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) was developed and optimized for the detection of type A influenza virus; the assay simultaneously differentiates avian H5, H7 and H9 hemagglutinin subtypes. Four sets of specific oligonucleotide primers were used in this test for type A influenza vi...

journal_title：Molecular and cellular probes

authors： Xie Z,Pang YS,Liu J,Deng X,Tang X,Sun J,Khan MI

更新日期：2006-06-01 00:00:00

abstract：:An oligonucleotide microarray was constructed for the rapid and sensitive molecular detection of antibiotic resistance determinants in Staphylococcus aureus. The array is equipped with oligonucleotide capture probes for the detection of 10 clinically and therapeutically relevant antibiotic resistance genes and -mutati...

journal_title：Molecular and cellular probes

authors： Strommenger B,Schmidt C,Werner G,Roessle-Lorch B,Bachmann TT,Witte W

更新日期：2007-06-01 00:00:00

abstract：:For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

journal_title：Molecular and cellular probes

authors： Ursi D,Ieven M,Van Bever HP,Goossens H

更新日期：1998-08-01 00:00:00

abstract：:The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...

journal_title：Molecular and cellular probes

authors： Russo S,Mencarelli M,Cavalleri F,Selicorni A,Cogliati F,Larizza L

更新日期：2003-12-01 00:00:00

abstract：:A polymerase chain reaction (PCR) protocol was developed that could specifically amplify a 520-bp region of the erythromycin resistant methylase (ermC) gene sequence. The identity of the PCR-amplified 520-bp DNA was confirmed by HinCII endonuclease restriction digestion, which produced the predicted 440-bp and 80-bp D...

journal_title：Molecular and cellular probes

authors： Nawaz MS,Khan AA,Cerniglia CE

更新日期：1997-10-01 00:00:00

abstract：:Swine are implicated as the principal animal reservoir for plasmid-bearing Yersinia enterocolitica (YEP(+)) strains that are pathogenic to humans. To evaluate the utility of the PCR for detection of YEP(+) strains in naturally-contaminated pig feces, samples were first enriched in Irgasan ticarcillin potassium chlorat...

journal_title：Molecular and cellular probes

authors： Bhaduri S

更新日期：2002-06-01 00:00:00

abstract：:We describe a rapid, automated method for direct detection of known single-base changes in genomic DNA. Fluorescence-based DNA minisequence analysis is employed in a template-dependent reaction which involves a single nucleotide extension of an oligonucleotide primer by the correct fluorescently-tagged dideoxynucleoti...

journal_title：Molecular and cellular probes

authors： Kobayashi M,Rappaport E,Blasband A,Semeraro A,Sartore M,Surrey S,Fortina P

更新日期：1995-06-01 00:00:00

abstract：:Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage in cell culture is performed. A total of 54 archived faecal samples were examined. The heteroge...

journal_title：Molecular and cellular probes

authors： Bolanaki E,Kottaridi C,Dedepsidis E,Kyriakopoulou Z,Pliaka V,Pratti A,Levidiotou-Stefanou S,Markoulatos P

更新日期：2008-06-01 00:00:00

abstract：:The impact that next-generation sequencing technology (NGS) is having on many aspects of molecular and cell biology, is becoming increasingly apparent. One of the most noticeable outcomes of the new technology in human genetics, has been the accelerated rate of identification of disease-causing genes. Especially for r...

journal_title：Molecular and cellular probes

authors： Morris-Rosendahl DJ,Kaindl AM

更新日期：2015-10-01 00:00:00

abstract：OBJECT:To investigate the role of circHIPK3 in melanoma. METHODS:Bioinformatics analysis and experiments including RT-qPCR, Pearson's correlation analysis, luciferase reporter, Western blot, and RIP assays were applied to explore the function and mechanism of circHIPK3 in melanoma. RESULTS:CircHIPK3 expression was st...

journal_title：Molecular and cellular probes

authors： Zhu X,Sun J

更新日期：2020-10-01 00:00:00

abstract：:The detection of hepatitis C virus (HCV) RNA by nested polymerase chain reaction (PCR) is believed to be the most reliable method to diagnose HCV infections. A pitfall of nested PCR is that it is prone to contamination. Single step reverse transcription-PCR (RT-PCR) was performed, prospectively, on 80 sera from 59 pat...

journal_title：Molecular and cellular probes

authors： Aslanzadeh J,Padilla BB,Shanley JD

更新日期：1996-06-01 00:00:00

abstract：:Variation amongst strains of Helicobacter pylori and Helicobacter mustelae was examined by DNA restriction endonuclease digestion and rRNA gene patterns generated using a non-radioactive probe. Chromosomal DNA was extracted from 30 cultures of H. pylori from human, Rhesus monkey and pig gastric mucosa, and from three ...

journal_title：Molecular and cellular probes

authors： Morgan DD,Owen RJ

更新日期：1990-08-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a prevalent pathogen worldwide and causes various respiratory infections. Although it is a critical pathogen in pediatric patients, it is unclear how it enters host cells. In this study, we focused on hMPV cell entry using two kinds of cell lines (Vero E6 and LLC-MK2), which are most co...

journal_title：Molecular and cellular probes

authors： Yang H,He H,Tan B,Liu E,Zhao X,Zhao Y

更新日期：2016-08-01 00:00:00

abstract：:The presence of human cytomegalovirus (HCMV) was tested in 388 cervicovaginal cells specimens obtained from the same number of pregnant women. HCMV was detected in 5.41%, 11.6% and 13.9% of these specimens by conventional culture, in situ DNA hybridization and polymerase chain reaction (PCR) methods, respectively. The...

journal_title：Molecular and cellular probes

authors： Yuan CF,Kao SM,Wang DC,Ng HT,Pao CC

更新日期：1990-12-01 00:00:00

abstract：:Characterization of antimicrobial resistance and virulence gene profiles provides important information on the potential pathogenicity of bacteria. This information can be used to facilitate prompt and effective treatment of bacterial infections. We developed and tested a PCR-based microarray platform for detecting vi...

journal_title：Molecular and cellular probes

authors： Chen S,Zhao S,McDermott PF,Schroeder CM,White DG,Meng J

更新日期：2005-06-01 00:00:00