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DNA microarray for the detection of therapeutically relevant antibiotic resistance determinants in clinical isolates of Staphylococcus aureus.

Abstract：

:An oligonucleotide microarray was constructed for the rapid and sensitive molecular detection of antibiotic resistance determinants in Staphylococcus aureus. The array is equipped with oligonucleotide capture probes for the detection of 10 clinically and therapeutically relevant antibiotic resistance genes and -mutations (mecA, aacA-aphD, tetK, tetM, vat(A), vat(B), vat(C), erm(A), erm(C), grlA-mutation) as well as several control probes. A microarray concept was established including multiplexed PCR amplification, DNA labeling, hybridization and data processing. This concept was applied to clinical Staphylococcus aureus isolates and results were concordant with those from standard genotypic and phenotypic resistance testing. Our microarray concept offers rapid and accurate identification of antibiotic resistance profiles. It is easily expandable and thus can be adapted to changing clinical and epidemiological requirements in clinical diagnosis as well as in epidemiological studies.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Strommenger B,Schmidt C,Werner G,Roessle-Lorch B,Bachmann TT,Witte W

doi

10.1016/j.mcp.2006.10.003

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

161-70

issue

3

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(06)00075-2

journal_volume

21

pub_type

杂志文章

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