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Recombinant VirB5 protein as a potential serological marker for the diagnosis of bovine brucellosis.

Abstract：

:The molecular tag vaccine against Brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. They can discriminate vaccinated animals and humans from those naturally infected. In this study, we constructed a gene deletion mutant strain, B. abortus S19 virB5 with a molecular tag. Recombinant VirB5 was expressed and purified for evaluation as a diagnostic reagent for bovine brucellosis. In total, 400 sera samples were tested using a VirB5 antigen-based enzyme-linked immunosorbent assay (ELISA) and the results were compared with those of the standard tube agglutination test (SAT). This showed that the sensitivity was 88.2%, specificity was 97.8% and accuracy was 94.8%. Recombinant VirB5 could also be used to discriminate B. abortus-infected mice from mice infected with the B. abortus S19 virB5 mutant strain. It was concluded that recombinant VirB5 could be used as a potential antigen and serological marker for the diagnosis of bovine brucellosis.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Tan W,Wang XR,Nie Y,Wang C,Cheng LQ,Wang XC,Zhang R,Yan GM

doi

10.1016/j.mcp.2012.02.003

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

127-31

issue

3

eissn

0890-8508

issn

1096-1194

journal_volume

26

pub_type

杂志文章

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