Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism.


:We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run using these primers, products were denatured and single-strand DNA fragments were subjected to electrophoresis. After silver staining, ssDNA band patterns characteristic for each genotype were observed. Minor differences within a serotype were detected without need for sequencing the PCR products making the preliminary analysis of avian influenza isolates much faster.


Mol Cell Probes


Gromadzka B,Smietanka K,Dragun J,Minta Z,Gora-Sochacka A,Szewczyk B




Has Abstract


2008-10-01 00:00:00














  • Quantitative real-time PCR assay for Clostridium septicum in poultry gangrenous dermatitis associated samples.

    abstract::Clostridium septicum is a spore-forming anaerobe frequently implicated in cases of gangrenous dermatitis (GD) and other spontaneously occurring myonecrotic infections of poultry. Although C. septicum is readily cultured from diseased tissues it can be difficult to enumerate due to its tendency to swarm over the surfac...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Neumann AP,Dunham SM,Rehberger TG,Siragusa GR

    更新日期:2010-08-01 00:00:00

  • Specific detection of common pathogens of acute bacterial meningitis using an internally controlled tetraplex-PCR assay.

    abstract::Accurate and timely diagnosis of acute bacterial meningitis is critical for antimicrobial treatment of patients. Although PCR-based methods have been widely used for the diagnosis of acute meningitis caused by bacterial pathogens, the main disadvantage of these methods is their high cost. This disadvantage has hampere...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Farahani H,Ghaznavi-Rad E,Mondanizadeh M,MirabSamiee S,Khansarinejad B

    更新日期:2016-08-01 00:00:00

  • What next-generation sequencing (NGS) technology has enabled us to learn about primary autosomal recessive microcephaly (MCPH).

    abstract::The impact that next-generation sequencing technology (NGS) is having on many aspects of molecular and cell biology, is becoming increasingly apparent. One of the most noticeable outcomes of the new technology in human genetics, has been the accelerated rate of identification of disease-causing genes. Especially for r...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Morris-Rosendahl DJ,Kaindl AM

    更新日期:2015-10-01 00:00:00

  • Rapid and visual detection of dengue virus using recombinase polymerase amplification method combined with lateral flow dipstick.

    abstract::Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

    更新日期:2019-08-01 00:00:00

  • Genetic variation of the methylenetetrahydrofolate reductase and cystathionine beta-synthase genes in Korean patients with coronary artery disease and a new polymorphism in intron 7.

    abstract::We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant d...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hong SH,Song J,Kim JQ

    更新日期:2001-04-01 00:00:00

  • A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction.

    abstract::Quantification of nucleic acids, especially of mRNA, is increasingly important in biomedical research. The recently developed quantitative real-time polymerase chain reaction (PCR) - a highly sensitive technology for the rapid, accurate and reproducible quantification of gene expression - offers major advantages over ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Fronhoffs S,Totzke G,Stier S,Wernert N,Rothe M,Brüning T,Koch B,Sachinidis A,Vetter H,Ko Y

    更新日期:2002-04-01 00:00:00

  • Genomic structure of the EPHA1 receptor tyrosine kinase gene.

    abstract::Some receptor tyrosine kinase genes are mutated in inherited and somatically acquired human cancers. To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions. The gene contains 18 exons, two...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Owshalimpur D,Kelley MJ

    更新日期:1999-06-01 00:00:00

  • Circ_016719 plays a critical role in neuron cell apoptosis induced by I/R via targeting miR-29c/Map2k6.

    abstract:BACKGROUND:Stroke is a leading cause of mortality worldwide. Rac-MAPK kinase 6 (Map2k6) plays important roles in cell proliferation and apoptosis. However, the role played by Map2k6 in stroke injury and the underlying mechanism of action remain unknown. METHODS:Mice received cerebral ischemia/reperfusion (I/R) injurie...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Tang C,Ou J,Kou L,Deng J,Luo S

    更新日期:2020-02-01 00:00:00

  • Down-regulation of UBA6 exacerbates brain injury by inhibiting the activation of Notch signaling pathway to promote cerebral cell apoptosis in rat acute cerebral infarction model.

    abstract::This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

    更新日期:2020-10-01 00:00:00

  • A novel highly informative polyA microsatellite on the telomeric side of the INK4a/ARF locus.

    abstract::The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Chaubert P,Burri N,Cousin P,Shaw P

    更新日期:2001-06-01 00:00:00

  • Microduplication of 10q26.3 in a Chinese hypertriglyceridemia patient.

    abstract::Hypertriglyceridemia (HTG) plays an important role in the development and progression of atherosclerosis. It is inherited in an autosomal dominant pattern with a frequency of approximately 1:1,000,000 worldwide. Previous study has demonstrated that more than six genes underlie this disorder. In addition, copy number v...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Li JJ,Chen YQ,Fan LL,Jin JY,Guo S,Xiang R

    更新日期:2018-02-01 00:00:00

  • Sensitive and quantitative detection of PCR-amplified HIV-1 DNA products by an enzyme linked immunoassay following solution hybridization with two differently labelled oligonucleotide probes.

    abstract::We have developed and evaluated an ELISA-based detection method for PCR-amplified HIV-1 DNA. The assay uses two oligonucleotide probes which are end-labelled at the 5'-end with biotin or digoxigenin, respectively. Upon solution hybridization of these probes which react with the same strand of amplified DNA product, th...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Böni J,Schüpbach J

    更新日期:1993-10-01 00:00:00

  • Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay.

    abstract::Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten b...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Liu CY,Song HQ,Zhang RL,Chen MX,Xu MJ,Ai L,Chen XG,Zhan XM,Liang SH,Yuan ZG,Lin RQ,Zhu XQ

    更新日期:2011-08-01 00:00:00

  • Quantification of sensitive non-isotopic filter hybridizations using the peroxidase catalyzed luminol reaction.

    abstract::The development of a sensitive, non-isotopic filter hybridization method based on the peroxidase catalyzed luminol reaction is described. High sensitivity was achieved by optimizing the conditions of the hybridization procedure, the immunochemical detection and the peroxidase/luminol reaction. This resulted in the rep...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: van Gijlswijk RP,Raap AK,Tanke HJ

    更新日期:1992-06-01 00:00:00

  • HyBeacon probes: a new tool for DNA sequence detection and allele discrimination.

    abstract::Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: French DJ,Archard CL,Brown T,McDowell DG

    更新日期:2001-12-01 00:00:00

  • DNA typing of HLA-B27 by polymerase chain reaction.

    abstract::To find a specific method for HLA-B27 typing, we tested an HLA-B27-specific polymerase chain reaction. This method was used for screening 100 randomly selected blood donors, 10 of them being HLA-B27 positive. A flow cytometric method and this PCR method were compared. ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lucotte G,Burckel A

    更新日期:1997-08-01 00:00:00

  • Development of a multiplex PCR assay for the identification of pathogenic genes of Escherichia coli in milk and milk products.

    abstract::A multiplex PCR for the simultaneous detection of some pathogenic genes of enteropathogenic, enterotoxigenic and verocytotoxin-producing Escherichia coli was developed. In this study primers found in literature as well as primers to the purpose designed were used. In this way, it was possible to generate specific frag...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bottero MT,Dalmasso A,Soglia D,Rosati S,Decastelli L,Civera T

    更新日期:2004-08-01 00:00:00

  • Fluorescence-based DNA minisequence analysis for detection of known single-base changes in genomic DNA.

    abstract::We describe a rapid, automated method for direct detection of known single-base changes in genomic DNA. Fluorescence-based DNA minisequence analysis is employed in a template-dependent reaction which involves a single nucleotide extension of an oligonucleotide primer by the correct fluorescently-tagged dideoxynucleoti...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Kobayashi M,Rappaport E,Blasband A,Semeraro A,Sartore M,Surrey S,Fortina P

    更新日期:1995-06-01 00:00:00

  • LncRNA GAS5 participates in the regulation of dexamethasone on androgen receptor -negative and -positive prostate cancer cell proliferation.

    abstract:BACKGROUND:Androgen receptor (AR) and long non-coding RNAs (lncRNA) play important roles in the initiation and progression of prostate cancer (PCa). The present study was designed to investigate whether lncRNA growth arrest-specific 5 (GAS5) is involved in the regulation of dexamethasone on the proliferation of AR+ PCa...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hu J,Deng J,Cao R,Xiong S,Guo J

    更新日期:2020-10-01 00:00:00

  • DNA hybridization for diagnosis of pertussis.

    abstract::The aim of the present study was to evaluate a mixed phase DNA hybridization assay for detection of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal aspirates from patients with suspected pertussis. Among 179 consecutive patients with own or parental suspicion of pertussis, the diagnosis was confirm...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Reizenstein E,Morfeldt E,Granström G,Granström M,Löfdahl S

    更新日期:1990-08-01 00:00:00

  • WDR45 mutations in Rett (-like) syndrome and developmental delay: Case report and an appraisal of the literature.

    abstract::Mutations in the WDR45 gene have been identified as causative for the only X-linked type of neurodegeneration with brain iron accumulation (NBIA), clinically characterized by global developmental delay in childhood, followed by a secondary neurological decline with parkinsonism and/or dementia in adolescence or early ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Hoffjan S,Ibisler A,Tschentscher A,Dekomien G,Bidinost C,Rosa AL

    更新日期:2016-02-01 00:00:00

  • A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR.

    abstract::Species identification of Mycobacterium tuberculosis remains a cumbersome process. We have developed a simple method for treating clinical samples which permits direct polymerase chain reaction (PCR) amplification of mycobacterial target DNA without organic extraction. Samples were boiled for 30 min in TE-Triton, then...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sritharan V,Barker RH Jr

    更新日期:1991-10-01 00:00:00

  • Quantitative PCR evaluation of deletions/duplications identified by array CGH.

    abstract::Genomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentia...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Baldan F,Passon N,Burra S,Demori E,Russo PD,Damante G

    更新日期:2019-08-01 00:00:00

  • MiR-34a suppresses the proliferation and invasion of gastric cancer by modulating PDL1 in the immune microenvironment.

    abstract:OBJECTIVES:As one of the most serious malignant carcinomas that threaten the life of sufferers constantly, gastric cancer has attracted a lot of interest among researchers. miR-34a, a member of hundreds of microRNAs (miRNAs), has been elucidated to exert a suppressive role in gastric cancer tumorgenesis based on previo...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yong H,Fu J,Gao G,Shi H,Zheng D,Zhou X

    更新日期:2020-10-01 00:00:00

  • Design considerations and effects of LNA in PCR primers.

    abstract::The effects of comprehensive LNA substitution in PCR primers for amplification of human genomic DNA targets are presented in this report. Previous research with LNA in other applications has shown interesting properties for molecular hybridization including enhanced specificity in allele-specific PCR. Here we systemat...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Latorra D,Arar K,Hurley JM

    更新日期:2003-10-01 00:00:00

  • Transcriptional profiles of regulatory and virulence factors of Staphylococcus aureus of bovine origin: oxygen impact and strain-to-strain variations.

    abstract::Staphylococcus aureus is responsible for a large panel of infections in humans and animals. In cows, S. aureus provokes chronic intramammary infections. Little information is available about the regulation of virulence factors in bovine isolates. Moreover, oxygenation, which is low in an inflamed mammary gland, could ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ster C,Gilbert FB,Cochard T,Poutrel B

    更新日期:2005-08-01 00:00:00

  • Selective growth of mosaic cells in chromosomal analysis of chorionic villi by conventional karyotyping.

    abstract::The major cause of first-trimester pregnancy loss is chromosomal abnormality, which could be detected by many methods. Conventional karyotyping based on chorionic villi (CV) culture is frequently used but may have limitations due to culture failure and selective growth of cells. In this study, we aimed to investigate ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Zhang Y,Lei Q,Liu J,Lin M,Luo L,Li T,Wang Q,Zhou C

    更新日期:2020-06-01 00:00:00

  • GT repeat polymorphism in the 5' flanking region of the human growth hormone receptor gene.

    abstract::A polymorphic GT dinucleotide repeat sequence has been identified in the 5' flanking region of the human growth hormone receptor (hGHR) gene on chromosome 5p13.1-p12, within the promoter region of the V9 5'UTR exon. Thirteen alleles have been identified in 50 non-related individuals, with an observed heterozygosity of...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hadjiyannakis S,Zheng H,Hendy GN,Goodyer CG

    更新日期:2001-08-01 00:00:00

  • Detection of catechol-O-methyltransferase Val158Met polymorphism by a simple one-step tetra-primer amplification refractory mutation system-PCR.

    abstract::The G-->A transition at nucleotide 21881 of the human catechol-O-methyltransferase (COMT) gene represents a functional genetic polymorphism (Val158Met), rendering an enzyme with reduced activity that has been associated with psychiatric disorders and estrogen-related cancers. A new method for the detection of this pol...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ruiz-Sanz JI,Aurrekoetxea I,Ruiz del Agua A,Ruiz-Larrea MB

    更新日期:2007-06-01 00:00:00

  • Specific detection of Campylobacter concisus by PCR amplification of 23S rDNA areas.

    abstract::The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. conc...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bastyns K,Chapelle S,Vandamme P,Goossens H,De Wachter R

    更新日期:1995-08-01 00:00:00