Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism.

Abstract:

:We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run using these primers, products were denatured and single-strand DNA fragments were subjected to electrophoresis. After silver staining, ssDNA band patterns characteristic for each genotype were observed. Minor differences within a serotype were detected without need for sequencing the PCR products making the preliminary analysis of avian influenza isolates much faster.

journal_name

Mol Cell Probes

authors

Gromadzka B,Smietanka K,Dragun J,Minta Z,Gora-Sochacka A,Szewczyk B

doi

10.1016/j.mcp.2008.09.003

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

301-4

issue

5-6

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(08)00058-3

journal_volume

22

pub_type

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