In situ hybridization.

abstract：:This study reports a significant up-regulation of bta-miR-146a and bta-miR-146b expression levels in bovine mammary tissues infected with subclinical, clinical and experimental mastitis. Potential target genes are involved in multiple immunological pathways. These results suggest a regulatory function of both miRNAs f...

journal_title：Molecular and cellular probes

authors： Wang XP,Luoreng ZM,Zan LS,Raza SH,Li F,Li N,Liu S

更新日期：2016-10-01 00:00:00

abstract：:Bacterial kidney disease in salmonid fish is caused by the slow-growing Gram-positive rod, Renibacterium salmoninarum. The partial sequence of 16S rRNA from R. salmoninarum was determined and compared with published bacterial 16S rRNA sequences. From this sequence information, a 30-bases-long oligonucleotide was desig...

journal_title：Molecular and cellular probes

authors： Mattsson JG,Gersdorf H,Jansson E,Hongslo T,Göbel UB,Johansson KE

更新日期：1993-02-01 00:00:00

abstract：:To explore the genetic divergence and phylogeny of Chinese indigenous sheep breeds, in the current study, we analyzed the polymorphisms of 5 structural loci in ten sheep populations, including Sishui Fur, Sunite, Wurank, Bayinbuluke, Altay, Small-Tailed Han, Wadi, Tan, Tong and Hu sheep. The data were then compared wi...

journal_title：Molecular and cellular probes

authors： Li M,Xia H,Chen D,Ji D,Kenji T,Li R,Liao X,Mao Y,Sun W,Geng R,Yang Z

更新日期：2018-02-01 00:00:00

abstract：:New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env...

journal_title：Molecular and cellular probes

authors： Sabunciyan S,Mandelberg N,Rabkin CS,Yolken R,Viscidi R

更新日期：2011-04-01 00:00:00

abstract：:We propose a newborn cystic fibrosis (CF) screening test based on the analysis of dried blood spot DNA by a strategy involving simple or multiplex denaturing gradient gel electrophoresis (DGGE) of PCR products of CFTR gene fragments, in conjunction with the immunoreactive-trypsin (IRT) assay. From May 1988 to May 1992...

journal_title：Molecular and cellular probes

authors： Audrézet MP,Costes B,Ghanem N,Fanen P,Verlingue C,Morin JF,Mercier B,Goossens M,Férec C

更新日期：1993-12-01 00:00:00

abstract：:The development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay specifically detected PEDV and PCV3, with no fluorescence detected for oth...

journal_title：Molecular and cellular probes

authors： Han HY,Zheng HH,Zhao Y,Tian RB,Xu PL,Hou HL,Chen HY,Yang MF

更新日期：2019-04-01 00:00:00

abstract：:Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA L...

journal_title：Molecular and cellular probes

authors： Yang Y,Qin X,Zhang W,Li Z,Zhang S,Li Y,Zhang Z

更新日期：2017-06-01 00:00:00

abstract：:The ligase detection reaction (LDR) is a highly specific genotyping method for single nucleotide variations. Although LDR typically discriminates single nucleotide polymorphism (SNP) alleles at the 3' end of so-called LDR discriminating probes, we designed probes in which the position of nucleotide differences for dis...

journal_title：Molecular and cellular probes

authors： Asari M,Omura T,Maseda C,Shiono H,Tasaki Y,Matsubara K,Shimizu K

更新日期：2010-12-01 00:00:00

abstract：:DNA samples of 2303 individuals from nine different population groups were screened for variant -175g-->t in the promoter region of the low-density lipoprotein receptor (LDLR) gene. The -175g-->t variant detected at carrier frequencies of 3-10% in different African population groups was absent in the Caucasian and Asi...

journal_title：Molecular and cellular probes

authors： Hoogendijk CF,Scholtz CL,Pimstone SM,Ehrenborg E,Kastelein JJ,Defesche JC,Thiart R,du Plessis L,de Villiers JN,Zaahl MG,Delport R,Rubinsztein DC,Raffel LJ,Grim CE,Mediene-Benchekor S,Amouyel P,Brousseau T,Steyn K,Lomb

更新日期：2003-08-01 00:00:00

abstract：:alpha-SNAP is an essential component of the protein machinery responsible for membrane fusion events in different cell types. The hyh (hydrocephalus with hop gait) mouse carries a missense mutation in Napa gene that results in a point mutation (M105I) in alpha-SNAP protein. Homozygous animals for the mutant allele hav...

journal_title：Molecular and cellular probes

authors： Bátiz LF,Roales-Buján R,Rodríguez-Pérez LM,Matas IM,Páez P,Roque M,Jiménez AJ,Ramos C,Pérez-Fígares JM

更新日期：2009-12-01 00:00:00

abstract：:Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 's...

journal_title：Molecular and cellular probes

authors： Peruzzi B,Serra M,Pescucci C,Sica M,Lastraioli S,Rondelli T,Pedemonte S,Risitano AM,De Angioletti M,Piccioli P,Notaro R

更新日期：2010-12-01 00:00:00

abstract：:An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures ...

journal_title：Molecular and cellular probes

authors： Chantratita W,Henchal EA,Yoosook C

更新日期：1989-12-01 00:00:00

abstract：:The spectrum of cystic fibrosis (CF) mutations has been determined in many populations of different ethnic and geographic origins. However, in the south of Europe, the commonest mutation, delta F508, accounts for only about 50% of CF chromosomes, while identification of most of the other mutant alleles has not been ac...

journal_title：Molecular and cellular probes

authors： Férec C,Novelli G,Verlingue C,Quéré I,Dallapiccola B,Audrézet MP,Mercier B

更新日期：1995-04-01 00:00:00

abstract：:The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...

journal_title：Molecular and cellular probes

authors： Russo S,Mencarelli M,Cavalleri F,Selicorni A,Cogliati F,Larizza L

更新日期：2003-12-01 00:00:00

abstract：:A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larva...

journal_title：Molecular and cellular probes

authors： Campbell BE,Rabelo EM,Hofmann A,Hu M,Gasser RB

更新日期：2010-08-01 00:00:00

abstract：:This report describes detailed taxonomic and phylogenetic analysis of 15 non-tuberculous mycobacteria (NTMs) isolated from human pathological specimens in a Caribbean setting (12 slow-growers and three rapid-growers) that were not identified by cultural and biochemical tests and drug-susceptibility results. These isol...

journal_title：Molecular and cellular probes

authors： Ferdinand S,Legrand E,Goh KS,Berchel M,Mazzarelli G,Sola C,Tortoli E,Rastogi N

更新日期：2004-12-01 00:00:00

abstract：:At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed...

journal_title：Molecular and cellular probes

authors： Newton LA,Chilton NB,Monti JR,Bjørn H,Várady M,Christensen CM,Gasser RB

更新日期：1997-04-01 00:00:00

abstract：:Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 196...

journal_title：Molecular and cellular probes

authors： Komissarov A,Fadeev A,Kosheleva A,Sintsova K,Grudinin M

更新日期：2017-10-01 00:00:00

abstract：:Non-small-cell lung carcinoma (NSCLC) accounts for approximately 80% of lung cancers with a high metastatic potential. Elucidating the mechanism of NSCLC metastasis will provide new promising targets for NSCLC therapy and benefit its prognosis. Plasmacytoma variant translocation 1 (PVT1) has been proven to be overexpr...

journal_title：Molecular and cellular probes

authors： Su XH,Zhu YR,Hou YJ,Li K,Dong NH

更新日期：2020-12-01 00:00:00

abstract：:Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

journal_title：Molecular and cellular probes

authors： Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

更新日期：2019-08-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was develop...

journal_title：Molecular and cellular probes

authors： Li R,Wang J,Liu L,Zhang R,Hao X,Han Q,Wang J,Yuan W

更新日期：2019-06-01 00:00:00

abstract：:We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing ...

journal_title：Molecular and cellular probes

authors： Zhu YC,Mitchell KK,Nazarian EJ,Escuyer VE,Musser KA

更新日期：2015-12-01 00:00:00

abstract：AIM:Though Endostar (ES) could inhibit tumor growth by inhibiting tumor angiogenesis, other possible mechanisms have been less reported. This study aims to investigate the role of ES in the treatment of lung cancer from the perspective of macrophage-mediated epithelial mesenchymal transformation (EMT). METHODS:THP1 ce...

journal_title：Molecular and cellular probes

authors： Shen Y,Chen Q,Li L

更新日期：2019-06-01 00:00:00

abstract：:We have developed a highly specific and sensitive technique for the detection of Pneumocystis carinii DNA using DNA amplification by the polymerase chain reaction (PCR). PCR products are detected by agarose gel electrophoresis and Southern hybridization to an oligonucleotide probe. Here we report the calibration of pa...

journal_title：Molecular and cellular probes

authors： Peters SE,Wakefield AE,Banerji S,Hopkin JM

更新日期：1992-04-01 00:00:00

abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-rea...

journal_title：Molecular and cellular probes

authors： Chen HT,Zhang J,Ma YP,Ma LN,Ding YZ,Liu XT,Cai XP,Ma LQ,Zhang YG,Liu YS

更新日期：2010-04-01 00:00:00

abstract：:The manual gravimetric drying moisture determination methods currently employed by most mineral processing plants fail to provide timely and accurate information required for automatic control. The costs associated with transporting and handling concentrates still represent a major portion of the overall treatment pri...

journal_title：Molecular and cellular probes

authors： Cancilla PA,Barrette P,Rosenblum F

更新日期：2002-12-01 00:00:00

abstract：:Rapid pathogen testing is vital to the food industry. Enzyme immunoassays (EIA) provide reliable negative results in 48 h, but a presumptive positive (suspect) EIA result must be confirmed by traditional culture methods, requiring an additional 72 h. Polymerase chain reaction (PCR) testing technology is accepted as an...

journal_title：Molecular and cellular probes

authors： Ellingson JL,Anderson JL,Carlson SA,Sharma VK

更新日期：2004-02-01 00:00:00

abstract：:Sepsis is a common complication in infection, trauma, and surgery. Severe sepsis has been identified as the leading cause of death in patients suffering from noncardiovascular ailments in intensive care units. In the current study, we used lipopolysaccharide (LPS) to stimulate the mouse macrophage cell line RAW264.7, ...

journal_title：Molecular and cellular probes

authors： Yang Q,Cao K,Jin G,Zhang J

更新日期：2019-10-01 00:00:00

abstract：:Genomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentia...

journal_title：Molecular and cellular probes

authors： Baldan F,Passon N,Burra S,Demori E,Russo PD,Damante G

更新日期：2019-08-01 00:00:00

abstract：:A rapid polymerase chain reaction (PCR) assay specific for Marek's Disease Virus (MDV) was developed. This assay was able to detect MDV in inoculated chick kidney cells at dilutions of 10(-5). Negative PCR results were obtained using uninoculated chick cells, Marek's Disease Vaccine (SB), Herpesvirus of Turkeys (HVT) ...

journal_title：Molecular and cellular probes

authors： Rong-Fu W,Beasley JN,Cao WW,Slavik MF,Johnson MG

更新日期：1993-04-01 00:00:00