Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes.

abstract：:To find a specific method for HLA-B27 typing, we tested an HLA-B27-specific polymerase chain reaction. This method was used for screening 100 randomly selected blood donors, 10 of them being HLA-B27 positive. A flow cytometric method and this PCR method were compared. ...

journal_title：Molecular and cellular probes

authors： Lucotte G,Burckel A

更新日期：1997-08-01 00:00:00

abstract：:We have examined the relationship between Chlamydia trachomatis found in clinical samples in which the cryptic plasmid was absent and known serovars of C. trachomatis. PCR and RNase protection assays were used to compare 12 C. trachomatis serovars and a plasmidless L2 serovar strain with the reactivity of clinical spe...

journal_title：Molecular and cellular probes

authors： An Q,Olive DM

更新日期：1994-10-01 00:00:00

abstract：:The hydroxymethylbilane synthase (HMBS) mRNAs from 44 control individuals and 30 patients suffering from acute intermittent porphyria (AIP), were screened for length differences by reverse transcriptase polymerase chain reaction (RT-PCR) and any abnormalities were characterized by direct sequencing. Examination of the...

journal_title：Molecular and cellular probes

authors： Ong PM,Lanyon WG,Moore MR,Connor JM

更新日期：1998-04-01 00:00:00

abstract：:5-Fluorouracil (5FU), an antimetabolite often used for the treatment of breast cancer, binds to and inactivates the enzyme thymidylate synthase (TS). Measurement of TS levels may be useful in examining resistance to 5FU, but current methods involving ligand binding assays present considerable problems due to the low b...

journal_title：Molecular and cellular probes

authors： Elledge RM,Okuyama N,Fitzgerald SD,Fugua SA

更新日期：1994-02-01 00:00:00

abstract：:Genomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentia...

journal_title：Molecular and cellular probes

authors： Baldan F,Passon N,Burra S,Demori E,Russo PD,Damante G

更新日期：2019-08-01 00:00:00

abstract：:Surveillance for vancomycin resistant enterococci (VRE) by culture can be labour intensive and time consuming. We have developed a multiplex polymerase chain reaction (MPCR) which can be performed directly on the clinical specimen. The assay allows sensitive detection of enterococci with vanA - and vanB -mediated resi...

journal_title：Molecular and cellular probes

authors： Petrich AK,Luinstra KE,Groves D,Chernesky MA,Mahony JB

更新日期：1999-08-01 00:00:00

abstract：:A multiplex PCR for the simultaneous detection of some pathogenic genes of enteropathogenic, enterotoxigenic and verocytotoxin-producing Escherichia coli was developed. In this study primers found in literature as well as primers to the purpose designed were used. In this way, it was possible to generate specific frag...

journal_title：Molecular and cellular probes

authors： Bottero MT,Dalmasso A,Soglia D,Rosati S,Decastelli L,Civera T

更新日期：2004-08-01 00:00:00

abstract：:Metastasis and recurrence of breast cancer remain significant clinical problems. The expression level of CD44 protein is higher in breast cancer-initiating cancer stem cells; therefore, the early detection of CD44 using a sensitive diagnostic probe is important for breast cancer diagnosis and therapeutic purposes. In ...

journal_title：Molecular and cellular probes

authors： Cho JH,Lee SC,Ha NR,Lee SJ,Yoon MY

更新日期：2015-12-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was develop...

journal_title：Molecular and cellular probes

authors： Li R,Wang J,Liu L,Zhang R,Hao X,Han Q,Wang J,Yuan W

更新日期：2019-06-01 00:00:00

abstract：:The G-->A transition at nucleotide 21881 of the human catechol-O-methyltransferase (COMT) gene represents a functional genetic polymorphism (Val158Met), rendering an enzyme with reduced activity that has been associated with psychiatric disorders and estrogen-related cancers. A new method for the detection of this pol...

journal_title：Molecular and cellular probes

authors： Ruiz-Sanz JI,Aurrekoetxea I,Ruiz del Agua A,Ruiz-Larrea MB

更新日期：2007-06-01 00:00:00

abstract：:Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

journal_title：Molecular and cellular probes

authors： Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

更新日期：2019-08-01 00:00:00

abstract：:Mutations in the WDR45 gene have been identified as causative for the only X-linked type of neurodegeneration with brain iron accumulation (NBIA), clinically characterized by global developmental delay in childhood, followed by a secondary neurological decline with parkinsonism and/or dementia in adolescence or early ...

journal_title：Molecular and cellular probes

authors： Hoffjan S,Ibisler A,Tschentscher A,Dekomien G,Bidinost C,Rosa AL

更新日期：2016-02-01 00:00:00

abstract：:Characterization of antimicrobial resistance and virulence gene profiles provides important information on the potential pathogenicity of bacteria. This information can be used to facilitate prompt and effective treatment of bacterial infections. We developed and tested a PCR-based microarray platform for detecting vi...

journal_title：Molecular and cellular probes

authors： Chen S,Zhao S,McDermott PF,Schroeder CM,White DG,Meng J

更新日期：2005-06-01 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) to detect shigellae, EIEC and ETEC in stool specimens of diarrhoeic patients returning from tropical countries. As compared to culture (7.1% positive specimens), which recognizes only Shigella strains, PCR performed on bacterial growth from directly inoculated MacConkey...

journal_title：Molecular and cellular probes

authors： Lüscher D,Altwegg M

更新日期：1994-08-01 00:00:00

abstract：:Holstein haplotype (HH) 1, 3 and 4 are lethal mutations, responsible for early embryonic losses in Holstein Friesian (HF) cattle, worldwide. Three PCR based assays - tetra Amplification Refractory Mutation System PCR, PCR primer induced restriction analysis and PCR-restriction fragment length polymorphism techniques f...

journal_title：Molecular and cellular probes

authors： Kumar A,Gupta ID,Mohan G,Vineeth MR,Ravi Kumar D,Jayakumar S,Niranjan SK

更新日期：2020-04-01 00:00:00

abstract：:To explore the genetic divergence and phylogeny of Chinese indigenous sheep breeds, in the current study, we analyzed the polymorphisms of 5 structural loci in ten sheep populations, including Sishui Fur, Sunite, Wurank, Bayinbuluke, Altay, Small-Tailed Han, Wadi, Tan, Tong and Hu sheep. The data were then compared wi...

journal_title：Molecular and cellular probes

authors： Li M,Xia H,Chen D,Ji D,Kenji T,Li R,Liao X,Mao Y,Sun W,Geng R,Yang Z

更新日期：2018-02-01 00:00:00

abstract：:Serum lipid profile which is determined by genotype-phenotype relationship plays a significant role in the development of cardiovascular disease. Upstream stimulatory factor 1 (USF1), has been reported to be associated with serum lipid levels in different population, hence, this study investigated the association of v...

journal_title：Molecular and cellular probes

authors： Onadeko OT,Okunowo WO,Imaga NOA,Abdulrazaq MM,Onuminya OJ,Van-Lare TO,Nwosu M

更新日期：2020-12-08 00:00:00

abstract：:Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasm...

journal_title：Molecular and cellular probes

authors： Wang H,Fadl AA,Khan MI

更新日期：1997-06-01 00:00:00

abstract：:The progressive myoclonus epilepsy of Lafora type (LD) is an autosomal recessive disorder caused by mutations in the EPM2A gene. We demonstrated recently that EPM2A encodes a dual-specificity phosphatase that is primarily associated with polyribosomes. In the present study, we screened for mutations in the EPM2A gene ...

journal_title：Molecular and cellular probes

authors： Ganesh S,Shoda K,Amano K,Uchiyama A,Kumada S,Moriyama N,Hirose S,Yamakawa K

更新日期：2001-10-01 00:00:00

abstract：:The development of a sensitive, non-isotopic filter hybridization method based on the peroxidase catalyzed luminol reaction is described. High sensitivity was achieved by optimizing the conditions of the hybridization procedure, the immunochemical detection and the peroxidase/luminol reaction. This resulted in the rep...

journal_title：Molecular and cellular probes

authors： van Gijlswijk RP,Raap AK,Tanke HJ

更新日期：1992-06-01 00:00:00

abstract：:Some receptor tyrosine kinase genes are mutated in inherited and somatically acquired human cancers. To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions. The gene contains 18 exons, two...

journal_title：Molecular and cellular probes

authors： Owshalimpur D,Kelley MJ

更新日期：1999-06-01 00:00:00

abstract：:Spinal muscular atrophy (SMA), the leading genetic cause of death in childhood, is an autosomal recessive neuromuscular disorder characterized by progressive muscle weakness, associated with deletions of the survival motor neuron 1 (SMN1) gene. Approximately 94% of SMA patients carry homologous deletions of SMN1 exon(...

journal_title：Molecular and cellular probes

authors： Baris I,Etlik O,Koksal V,Arican-Baris ST

更新日期：2010-06-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) is responsible for severe infections in immunocompromised patients. Viral load has recently been identified as one of the major risk factors for subsequent development of HCMV disease. In this context, we developed a protocol allowing rapid, sensitive and precise quantification of HCMV DNA...

journal_title：Molecular and cellular probes

authors： Poirier-Toulemonde AS,Imbert-Marcille BM,Ferré-Aubineau V,Besse B,Le Roux MG,Cantarovich D,Billaudel S

更新日期：1997-02-01 00:00:00

abstract：:Natalizumab is a humanized monoclonal antibody against the alpha4 chain of the alpha4beta1 and alpha4beta7 integrin heterodimers used with high effectiveness in the treatment of multiple sclerosis. The use of this drug can unfortunately be associated with the onset of progressive multifocal leukoencephalopathy, a poss...

journal_title：Molecular and cellular probes

authors： Rossi F,Newsome SD,Viscidi R

更新日期：2015-02-01 00:00:00

abstract：:Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three reactions, through SYBR green I melting curve analysis. The method utilizes type-specific primer se...

journal_title：Molecular and cellular probes

authors： Tsakogiannis D,Papacharalampous M,Toska E,Kyriakopoulou Z,Dimitriou TG,Ruether IG,Komiotis D,Markoulatos P

更新日期：2015-02-01 00:00:00

abstract：:Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficult. Reverse transcription plus polymerase chain reaction (RT-PCR) was ...

journal_title：Molecular and cellular probes

authors： Wang RF,Campbell WL,Cao WW,Colvert RM,Holland MA,Cerniglia CE

更新日期：1999-02-01 00:00:00

abstract：:An oligonucleotide microarray was constructed for the rapid and sensitive molecular detection of antibiotic resistance determinants in Staphylococcus aureus. The array is equipped with oligonucleotide capture probes for the detection of 10 clinically and therapeutically relevant antibiotic resistance genes and -mutati...

journal_title：Molecular and cellular probes

authors： Strommenger B,Schmidt C,Werner G,Roessle-Lorch B,Bachmann TT,Witte W

更新日期：2007-06-01 00:00:00

abstract：:A simple and rapid method, PCR-restriction enzyme analysis (PCR-RE) for BK virus (BKV) typing was developed, based on the presence of type-specific restriction enzyme sites in a 327 bp PCR-generated fragment which partially encodes the VP1 protein. The enzymes, Alu I, Xmn I and Ava II, were used to digest the PCR prod...

journal_title：Molecular and cellular probes

authors： Jin L

更新日期：1993-08-01 00:00:00

abstract：:The human respiratory syncytial virus is a common respiratory pathogen in children. Improved diagnosis of the virus is dependent on the development of tools for the rapid detection and estimation of the viral loads. In the current study, RT-qPCR using TaqMan hydrolysis probe based on the F gene detection was developed...

journal_title：Molecular and cellular probes

authors： Abdel-Moneim AS,Shehab GM,Alsulaimani AA,Al-Malky MIR

更新日期：2017-06-01 00:00:00

abstract：:Previous studies have demonstrated that insulin-like growth factor-I (IGF-1) and reactive oxygen species (ROS) are involved in the development and progression of various cancers. However, their regulatory mechanism remains unknown. In this study, we treated cancer cells (HeLa, HepG2 and SW1116 cells) and normal cells ...

journal_title：Molecular and cellular probes

authors： Wang C,An Y,Wang Y,Shen K,Wang X,Luan W,Ma F,Ni L,Liu M,Yu L

更新日期：2020-08-01 00:00:00