Quantification of sensitive non-isotopic filter hybridizations using the peroxidase catalyzed luminol reaction.

Abstract:

:The development of a sensitive, non-isotopic filter hybridization method based on the peroxidase catalyzed luminol reaction is described. High sensitivity was achieved by optimizing the conditions of the hybridization procedure, the immunochemical detection and the peroxidase/luminol reaction. This resulted in the reproducible detection of 10-30 femtogram of target DNA on blots within minutes when a cooled charge coupled device (CCD) camera was used to record the luminescence signal. After optimalization, the system was successfully applied for the qualitative and quantitative analysis of small amounts of DNA in dot-blots as well as in Southern blot analysis.

journal_name

Mol Cell Probes

authors

van Gijlswijk RP,Raap AK,Tanke HJ

doi

10.1016/0890-8508(92)90020-x

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

223-30

issue

3

eissn

0890-8508

issn

1096-1194

pii

0890-8508(92)90020-X

journal_volume

6

pub_type

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