DNA typing of HLA-B27 by polymerase chain reaction.

abstract：:PCR detection and quantification of vaginal lactobacilli remains problematic because of the high level of genetic heterogeneity and taxonomic complexity within the genus Lactobacillus. The aim of the present study was to identify conserved sequences among the genomes of major species of vaginal lactobacilli that could...

journal_title：Molecular and cellular probes

authors： Demkin VV,Koshechkin SI,Slesarev A

更新日期：2017-04-01 00:00:00

abstract：:The RNA genome of pathogenic and non-pathogenic variants of citrus Hop stunt viroid (HSVd) differ by five to six nucleotides located within the variable (V) domain referred to as the "cachexia expression motif". Sensitive hosts such as mandarin and its hybrids are seriously affected by cachexia disease. Current method...

journal_title：Molecular and cellular probes

authors： Loconsole G,Onelge N,Yokomi RK,Kubaa RA,Savino V,Saponari M

更新日期：2013-10-01 00:00:00

abstract：:This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

journal_title：Molecular and cellular probes

authors： Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

更新日期：2020-10-01 00:00:00

abstract：:Spinal muscular atrophy (SMA), the leading genetic cause of death in childhood, is an autosomal recessive neuromuscular disorder characterized by progressive muscle weakness, associated with deletions of the survival motor neuron 1 (SMN1) gene. Approximately 94% of SMA patients carry homologous deletions of SMN1 exon(...

journal_title：Molecular and cellular probes

authors： Baris I,Etlik O,Koksal V,Arican-Baris ST

更新日期：2010-06-01 00:00:00

abstract：:Variation amongst strains of Helicobacter pylori and Helicobacter mustelae was examined by DNA restriction endonuclease digestion and rRNA gene patterns generated using a non-radioactive probe. Chromosomal DNA was extracted from 30 cultures of H. pylori from human, Rhesus monkey and pig gastric mucosa, and from three ...

journal_title：Molecular and cellular probes

authors： Morgan DD,Owen RJ

更新日期：1990-08-01 00:00:00

abstract：:A beacon probe was designed to detect one of the two documented single nucleotide changes in IS481 target allele of Bordetella holmesii genome as compared to Bordetella pertussis. PCR amplified product targeting a region of IS481 in presence of the probe was subjected to a post-PCR hybridization and melting cycle. Hyb...

journal_title：Molecular and cellular probes

authors： Poddar SK

更新日期：2003-04-01 00:00:00

abstract：:Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is a highly contagious intestinal disease, resulting in substantial economic losses to the swine industry worldwide. In this study, three assays, namely a conventional reverse transcription-polymerase chain reaction (RT-PCR), a SYBR Green...

journal_title：Molecular and cellular probes

authors： Zhou X,Zhang T,Song D,Huang T,Peng Q,Chen Y,Li A,Zhang F,Wu Q,Ye Y,Tang Y

更新日期：2017-06-01 00:00:00

abstract：:Dengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA)...

journal_title：Molecular and cellular probes

authors： Xi Y,Xu CZ,Xie ZZ,Zhu DL,Dong JM

更新日期：2019-08-01 00:00:00

abstract：:We have developed a new methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase (TdT) using a thioflavin T probe. This method is highly selective and sensitive. The fluorescence intensity was direct proportion to Dam MTase concentration in...

journal_title：Molecular and cellular probes

authors： Ma C,Liu H,Li W,Chen H,Jin S,Wang J,Wang J

更新日期：2016-04-01 00:00:00

abstract：:Rapid detection of single-base changes is fundamental to molecular medicine. PCR amplification of specific alleles (PASA) has previously been used as a rapid method of genotyping single-nucleotide changes, but one reaction is required for each allele. This paper describes a Bidirectional PASA (Bi-PASA) method, which w...

journal_title：Molecular and cellular probes

authors： Lundberg GA

更新日期：2007-06-01 00:00:00

abstract：:Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myoc...

journal_title：Molecular and cellular probes

authors： Xia X,Liang Y,Zheng W,Lin D,Sun S

更新日期：2020-08-01 00:00:00

abstract：:Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

journal_title：Molecular and cellular probes

authors： Bang DD,Wedderkopp A,Pedersen K,Madsen M

更新日期：2002-10-01 00:00:00

abstract：:Infection of the cervix uteri with various types of human papillomaviruses is generally considered a necessary factor in the etiology of cancer of the cervix uteri. In many human populations throughout the world, approximately 90% of cervical carcinomas are found to harbour HPV genomes, as judged by Southern blot hybr...

journal_title：Molecular and cellular probes

authors： Chow V,Tham KM,Yeo-Gloss M,Lim-Tan SK,Sng I,Thirumoorthy T,Bernard HU

更新日期：1990-04-01 00:00:00

abstract：:Clostridium septicum is a spore-forming anaerobe frequently implicated in cases of gangrenous dermatitis (GD) and other spontaneously occurring myonecrotic infections of poultry. Although C. septicum is readily cultured from diseased tissues it can be difficult to enumerate due to its tendency to swarm over the surfac...

journal_title：Molecular and cellular probes

authors： Neumann AP,Dunham SM,Rehberger TG,Siragusa GR

更新日期：2010-08-01 00:00:00

abstract：:Yersinia pestis, the causative agent of plague mainly infects rodents, while humans are the accidental host. The conventional diagnostic methods available for Y. pestis exhibit cross-reactivity with other enteropathogenic bacteria which makes its detection difficult. Rapid and reliable point-of-care detection of Y. pe...

journal_title：Molecular and cellular probes

authors： Singh R,Pal V,Tripathi NK,Goel AK

更新日期：2020-12-01 00:00:00

abstract：:We have developed and evaluated an ELISA-based detection method for PCR-amplified HIV-1 DNA. The assay uses two oligonucleotide probes which are end-labelled at the 5'-end with biotin or digoxigenin, respectively. Upon solution hybridization of these probes which react with the same strand of amplified DNA product, th...

journal_title：Molecular and cellular probes

authors： Böni J,Schüpbach J

更新日期：1993-10-01 00:00:00

abstract：:Holstein haplotype (HH) 1, 3 and 4 are lethal mutations, responsible for early embryonic losses in Holstein Friesian (HF) cattle, worldwide. Three PCR based assays - tetra Amplification Refractory Mutation System PCR, PCR primer induced restriction analysis and PCR-restriction fragment length polymorphism techniques f...

journal_title：Molecular and cellular probes

authors： Kumar A,Gupta ID,Mohan G,Vineeth MR,Ravi Kumar D,Jayakumar S,Niranjan SK

更新日期：2020-04-01 00:00:00

abstract：:This report describes detailed taxonomic and phylogenetic analysis of 15 non-tuberculous mycobacteria (NTMs) isolated from human pathological specimens in a Caribbean setting (12 slow-growers and three rapid-growers) that were not identified by cultural and biochemical tests and drug-susceptibility results. These isol...

journal_title：Molecular and cellular probes

authors： Ferdinand S,Legrand E,Goh KS,Berchel M,Mazzarelli G,Sola C,Tortoli E,Rastogi N

更新日期：2004-12-01 00:00:00

abstract：:A nucleic acid hybridization assay for the detection of the pilin gene of Neisseria gonorrhoeae has been devised. The method involves solution hybridization of pilin specific synthetic oligonucleotide probes to genomic DNA in crude cell lysates. This is followed by capture of the probe-target complex onto a microtitre...

journal_title：Molecular and cellular probes

authors： Kolberg JA,Besemer DJ,Stempien MM,Urdea MS

更新日期：1989-03-01 00:00:00

abstract：:We have examined the relationship between Chlamydia trachomatis found in clinical samples in which the cryptic plasmid was absent and known serovars of C. trachomatis. PCR and RNase protection assays were used to compare 12 C. trachomatis serovars and a plasmidless L2 serovar strain with the reactivity of clinical spe...

journal_title：Molecular and cellular probes

authors： An Q,Olive DM

更新日期：1994-10-01 00:00:00

abstract：:The G-->A transition at nucleotide 21881 of the human catechol-O-methyltransferase (COMT) gene represents a functional genetic polymorphism (Val158Met), rendering an enzyme with reduced activity that has been associated with psychiatric disorders and estrogen-related cancers. A new method for the detection of this pol...

journal_title：Molecular and cellular probes

authors： Ruiz-Sanz JI,Aurrekoetxea I,Ruiz del Agua A,Ruiz-Larrea MB

更新日期：2007-06-01 00:00:00

abstract：:Single nucleotide polymorphisms (SNPs) can significantly contribute to the cellular level of the mRNA transcripts encoded by human disease related genes. DNA variations between individuals can be an indication of predisposition to disease or affect the response to treatment. An algorithm allowing in silico extraction ...

journal_title：Molecular and cellular probes

authors： Stepanova M,Tiazhelova T,Skoblov M,Baranova A

更新日期：2006-12-01 00:00:00

abstract：:A DNA amplification procedure using heat stable Taq polymerase and the polymerase chain reaction is described for the detection of Pseudomonas aeruginosa in specimens from cystic fibrosis patients. A set of primers was selected on the basis of the nucleotide sequence of the algD gene encoding GDP mannose dehydrogenase...

journal_title：Molecular and cellular probes

authors： McIntosh I,Govan JR,Brock DJ

更新日期：1992-08-01 00:00:00

abstract：:The aim of this study was to determine the presence of haematogenous neoplastic cells in patients with prostate cancer. Circulating prostate cells can be detected in cancer patients by using a nested-reverse transcriptase-polymerase chain reaction assay (RT-PCR), for prostate-specific membrane (PSM) antigen mRNA. This...

journal_title：Molecular and cellular probes

authors： Lucotte G,Mercier G,Burckel A

更新日期：1998-12-01 00:00:00

abstract：:Sepsis is a common complication in infection, trauma, and surgery. Severe sepsis has been identified as the leading cause of death in patients suffering from noncardiovascular ailments in intensive care units. In the current study, we used lipopolysaccharide (LPS) to stimulate the mouse macrophage cell line RAW264.7, ...

journal_title：Molecular and cellular probes

authors： Yang Q,Cao K,Jin G,Zhang J

更新日期：2019-10-01 00:00:00

abstract：:A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for direct identification of Pseudomonas aeruginosa from positive BACTEC blood culture bottles. PCR primers were designed to target a 249 bp sequence of the oprI gene in P. aeruginosa. Biotin-labeled probe (PA3) targeted to the species-specific motif we...

journal_title：Molecular and cellular probes

authors： Kurupati P,Kumarasinghe G,Laa Poh C

更新日期：2005-12-01 00:00:00

abstract：:Non-pathogenic Burkholderia thailandensis may be used as a model for Burkholderia pseudomallei due to the genetic similarity of these species. Moreover, the experimental manipulation of B. thailandensis is safer. In this study, we constructed recombinant flagellin protein fragments of B. thailandensis E264 (FLAG300, F...

journal_title：Molecular and cellular probes

authors： Wajanarogana S,Nimnuch P,Thongmee A,Kritsiriwuthinan K

更新日期：2013-04-01 00:00:00

abstract：:The human respiratory syncytial virus is a common respiratory pathogen in children. Improved diagnosis of the virus is dependent on the development of tools for the rapid detection and estimation of the viral loads. In the current study, RT-qPCR using TaqMan hydrolysis probe based on the F gene detection was developed...

journal_title：Molecular and cellular probes

authors： Abdel-Moneim AS,Shehab GM,Alsulaimani AA,Al-Malky MIR

更新日期：2017-06-01 00:00:00

abstract：:The development of a sensitive, non-isotopic filter hybridization method based on the peroxidase catalyzed luminol reaction is described. High sensitivity was achieved by optimizing the conditions of the hybridization procedure, the immunochemical detection and the peroxidase/luminol reaction. This resulted in the rep...

journal_title：Molecular and cellular probes

authors： van Gijlswijk RP,Raap AK,Tanke HJ

更新日期：1992-06-01 00:00:00

abstract：:Genetic polymorphism among patients with acute lymphoblastic leukemia (ALL) is an important factor in the effectiveness and toxicity of anti-leukemic drugs. Genotyping of various polymorphisms that impact the outcome of anti-leukemic drug therapy (pharmacogenetics) presents an attractive approach for developing indivi...

journal_title：Molecular and cellular probes

authors： Kang HJ,Oh Y,Chun SM,Seo YJ,Shin HY,Kim CW,Ahn HS,Han BD

更新日期：2008-06-01 00:00:00