当前位置： SCI文献检索 > MOLECULAR AND CELLULAR PROBES期刊下所有文献 > Characterization of a Caenorhabditis elegans glc seven-like phosphatase (gsp) orthologue from Haemonchus contortus (Nematoda).

Characterization of a Caenorhabditis elegans glc seven-like phosphatase (gsp) orthologue from Haemonchus contortus (Nematoda).

Abstract：

:A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larval stages or eggs. The full-length gene (2854 bp) contained ten exons and nine introns, and encoded a cDNA of 951 bp. Comparisons of the conceptually translated protein (316 amino acids, estimated at approximately 35 kDa) with serine/threonine phosphatases (STPs) from other organisms revealed the presence of the conserved motif LRGNHE. Structural analysis, by comparative modelling, confirmed strict conservation of residues and features involved in catalytic activity, and variation in the ligand-binding interface. Phylogenetic analysis of amino acid sequence data revealed that Hc-STP-1 clustered with STPs from other nematodes (including Caenorhabditis elegans, Trichostrongylus vitrinus, Oesophagostomum dentatum, Ascaris suum and Brugia malayi) to the exclusion of STPs from other organisms. The protein was inferred to be most closely related to the PP1 class of STPs of C. elegans, within a group containing STPs encoded, amongst others, by the genes gsp-3 and gsp-4 in this free-living nematode. The functions of proteins GSP-3 and GSP-4 are known to be central to spermatogenesis and other male-specific processes in C. elegans. The findings from the present and previous studies support the proposal that Hc-stp-1 and its product play a significant role in reproductive and/or developmental processes in maturing or adult male H. contortus.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Campbell BE,Rabelo EM,Hofmann A,Hu M,Gasser RB

doi

10.1016/j.mcp.2010.02.001

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

178-89

issue

4

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(10)00006-X

journal_volume

24

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR PROBES文献大全
  • Detection of adenovirus in the waters of the Seine River estuary by nested-PCR.

    abstract：:Several systems for isolating viruses from environmental samples have been tested. The most promising method is based on genomic amplification. The authors attempted to detect adenovirus in nucleic-acid extracts from the Seine River estuary by a two-step amplification of a 220-bp segment of the conserved coding region...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1998.0166

    authors： Castignolles N,Petit F,Mendel I,Simon L,Cattolico L,Buffet-Janvresse C

    更新日期：1998-06-01 00:00:00

  • No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls.

    abstract：:New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2011.01.005

    authors： Sabunciyan S,Mandelberg N,Rabkin CS,Yolken R,Viscidi R

    更新日期：2011-04-01 00:00:00

  • Molecular contrast of EGFR expression using gold nanoparticles as a reflectance-based imaging probe.

    abstract：:Advanced reflectance-based optical techniques for in vivo imaging often suffer from low contrast between neoplastic and normal tissue and are unable to image early biomolecular changes associated with carcinogenesis, thus limiting their clinical value. In this study, we exploit the resonance light scattering property ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2007.06.010

    authors： Kah JC,Olivo MC,Lee CG,Sheppard CJ

    更新日期：2008-02-01 00:00:00

  • Identification of crucial regulatory relationships between long non-coding RNAs and protein-coding genes in lung squamous cell carcinoma.

    abstract：PURPOSE:This study aimed to analyze the relationships of long non-coding RNAs (lncRNAs) and protein-coding genes in lung squamous cell carcinoma (LUSC). METHODS:RNA-seq data of LUSC deposited in the TCGA database were used to identify differentially expressed protein-coding genes (DECGs) and differentially expressed l...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2016.02.009

    authors： Wu X,Ruan L,Yang Y,Mei Q

    更新日期：2016-06-01 00:00:00

  • Tracking the extramedullary PML-RARα-positive cell reservoirs in a preclinical model: biomarker of long-term drug efficacy.

    abstract：:Using an acute promyelocytic leukemia (APL) preclinical model, we show that oncogene-specific PCR (Polymerase Chain Reaction)-based assays allow to evaluate the efficacy of immunotherapy combining all-trans retinoic acid (ATRA) and a DNA-based vaccine targeting the promyelocytic leukemia-retinoic acid receptor alpha (...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2012.08.001

    authors： Pokorna K,Le Pogam C,Chopin M,Balitrand N,Reboul M,Cassinat B,Chomienne C,Padua RA,Pla M

    更新日期：2013-02-01 00:00:00

  • Identification of six novel CFTR mutations in a sample of Italian cystic fibrosis patients.

    abstract：:The spectrum of cystic fibrosis (CF) mutations has been determined in many populations of different ethnic and geographic origins. However, in the south of Europe, the commonest mutation, delta F508, accounts for only about 50% of CF chromosomes, while identification of most of the other mutant alleles has not been ac...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(95)80038-7

    authors： Férec C,Novelli G,Verlingue C,Quéré I,Dallapiccola B,Audrézet MP,Mercier B

    更新日期：1995-04-01 00:00:00

  • Impact of fixation artifacts and threshold selection on high resolution melting analysis for KRAS mutation screening.

    abstract：INTRODUCTION:Treatment in metastatic colorectal cancer (mCRC) has expanded with monoclonal antibodies targeting epidermal growth factor receptor, but is restricted to patients with a wild-type (WT) KRAS mutational status. The most sensitive assays for KRAS mutation detection in formalin-fixed paraffin embedded (FFPE) t...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2017.06.003

    authors： Pérez-Báez W,García-Latorre EA,Maldonado-Martínez HA,Coronado-Martínez I,Flores-García L,Taja-Chayeb L

    更新日期：2017-10-01 00:00:00

  • PVT1 induces NSCLC cell migration and invasion by regulating IL-6 via sponging miR-760.

    abstract：:Non-small-cell lung carcinoma (NSCLC) accounts for approximately 80% of lung cancers with a high metastatic potential. Elucidating the mechanism of NSCLC metastasis will provide new promising targets for NSCLC therapy and benefit its prognosis. Plasmacytoma variant translocation 1 (PVT1) has been proven to be overexpr...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101652

    authors： Su XH,Zhu YR,Hou YJ,Li K,Dong NH

    更新日期：2020-12-01 00:00:00

  • Direct detection of vanA and vanB genes in clinical specimens for rapid identification of vancomycin resistant enterococci (VRE) using multiplex PCR.

    abstract：:Surveillance for vancomycin resistant enterococci (VRE) by culture can be labour intensive and time consuming. We have developed a multiplex polymerase chain reaction (MPCR) which can be performed directly on the clinical specimen. The assay allows sensitive detection of enterococci with vanA - and vanB -mediated resi...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1999.0250

    authors： Petrich AK,Luinstra KE,Groves D,Chernesky MA,Mahony JB

    更新日期：1999-08-01 00:00:00

  • Rapid genomic typing of BK virus directly from clinical specimens.

    abstract：:A simple and rapid method, PCR-restriction enzyme analysis (PCR-RE) for BK virus (BKV) typing was developed, based on the presence of type-specific restriction enzyme sites in a 327 bp PCR-generated fragment which partially encodes the VP1 protein. The enzymes, Alu I, Xmn I and Ava II, were used to digest the PCR prod...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1993.1047

    authors： Jin L

    更新日期：1993-08-01 00:00:00

  • Quantitative real-time PCR assay for Clostridium septicum in poultry gangrenous dermatitis associated samples.

    abstract：:Clostridium septicum is a spore-forming anaerobe frequently implicated in cases of gangrenous dermatitis (GD) and other spontaneously occurring myonecrotic infections of poultry. Although C. septicum is readily cultured from diseased tissues it can be difficult to enumerate due to its tendency to swarm over the surfac...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2010.04.001

    authors： Neumann AP,Dunham SM,Rehberger TG,Siragusa GR

    更新日期：2010-08-01 00:00:00

  • A fluorescent method for detecting low-grade 11patUPD mosaicism in Beckwith-Wiedemann syndrome.

    abstract：:The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2003.07.002

    authors： Russo S,Mencarelli M,Cavalleri F,Selicorni A,Cogliati F,Larizza L

    更新日期：2003-12-01 00:00:00

  • Opportunities and challenges for the application of microfluidic technologies in point-of-care veterinary diagnostics.

    abstract：:There is a growing need for low-cost, rapid and reliable diagnostic results in veterinary medicine. Point-of-care (POC) tests have tremendous advantages over existing laboratory-based tests, due to their intrinsic low-cost and rapidity. A considerable number of POC tests are presently available, mostly in dipstick or ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章,评审

    doi：10.1016/j.mcp.2016.07.004

    authors： Busin V,Wells B,Kersaudy-Kerhoas M,Shu W,Burgess ST

    更新日期：2016-10-01 00:00:00

  • Alternate PCR assays for screening of JH1 mutation associated with embryonic death in Jersey cattle.

    abstract：:Jersey haplotype (JH) 1, a stop-gain lethal mutation in the CWC15 gene, causes embryonic losses in Jersey cattle. Two PCR based assays using Amplification Refractory Mutation System (T-ARMS-PCR) and restriction fragment length polymorphism (PCR-RFLP) were developed for screening of the JH1 in cattle. During the screen...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101688

    authors： Kumar A,Gupta ID,Mohan G,M R V,D RK,S J,Kataria RS,Niranjan SK

    更新日期：2020-12-03 00:00:00

  • SNP genotyping with FRET probes. Optimizing the resolution of heterozygotes.

    abstract：:Analysis of single nucleotide polymorphisms by PCR with fluorescence resonance energy transfer (FRET) probes often can produce a result where the melting peak corresponding to perfectly matched sequence (A allele) has a smaller area than the peak corresponding to the allele with a mismatch (B allele). This imbalance c...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2004.03.003

    authors： Martínez-García A,Sastre I,Tenorio R,Bullido MJ

    更新日期：2004-08-01 00:00:00

  • Evaluation of two aneuploidy screening tests for chorionic villus samples: Multiplex ligation-dependent probe amplification and fluorescence in situ hybridization.

    abstract：:The vast majority of first-trimester pregnancy losses are the consequence of numerical aberrations in fetal chromosomes, which may involve nearly all chromosomes. Although commercial probes for all chromosomes are available for multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridizat...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.101422

    authors： Wu T,Zhu Y,Hong L,Lin Q,Chen C,Yang J,Ye L,Huang W,Zeng Y

    更新日期：2019-08-01 00:00:00

  • A novel peptide-based recognition probe for the sensitive detection of CD44 on breast cancer stem cells.

    abstract：:Metastasis and recurrence of breast cancer remain significant clinical problems. The expression level of CD44 protein is higher in breast cancer-initiating cancer stem cells; therefore, the early detection of CD44 using a sensitive diagnostic probe is important for breast cancer diagnosis and therapeutic purposes. In ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2015.05.014

    authors： Cho JH,Lee SC,Ha NR,Lee SJ,Yoon MY

    更新日期：2015-12-01 00:00:00

  • Real-time monitoring of DNA methyltransferase activity using a hemimethylated smart probe.

    abstract：:A real-time assay for DNA methyltransferase (MTase) activity has been developed. A hemimethylated smart probe is used as the substrate for DNA MTase. Cleavage of the methylated product leads to separation of fluorophore from quencher, giving a proportional increase in fluorescence. The method permits real-time monitor...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2016.03.007

    authors： Jin S,Liu H,Xia K,Ma C,He H,Wang K

    更新日期：2016-06-01 00:00:00

  • Evaluation of a DNA probe of plasmid origin for the detection of Chlamydia trachomatis in cultures and clinical specimens.

    abstract：:This study evaluates five cryptic plasmid-derived DNA probes in a 4-h slot-blot hybridization assay for the detection of Chlamydia trachomatis in cultures and clinical specimens. The probes, consisting of either the entire cloned 7.5 kbp cryptic plasmid pSE8 or one of four Hin dIII/Eco RI fragments measuring 710, 1055...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(05)80013-1

    authors： Cano RJ,Murrieta CM,Spaulding DC,Pascual A

    更新日期：1991-12-01 00:00:00

  • On-line moisture determination of ore concentrates 'a review of traditional methods and introduction of a novel solution'.

    abstract：:The manual gravimetric drying moisture determination methods currently employed by most mineral processing plants fail to provide timely and accurate information required for automatic control. The costs associated with transporting and handling concentrates still represent a major portion of the overall treatment pri...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章,评审

    doi：10.1006/mcpr.2002.0444

    authors： Cancilla PA,Barrette P,Rosenblum F

    更新日期：2002-12-01 00:00:00

  • A duplex PCR assay for the simultaneous detection and differentiation of Muscovy duck parvovirus and goose parvovirus.

    abstract：:Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific prim...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.101439

    authors： Wan C,Cheng L,Chen C,Liu R,Shi S,Fu G,Chen H,Fu Q,Huang Y

    更新日期：2019-10-01 00:00:00

  • Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes.

    abstract：:An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(89)90015-7

    authors： Chantratita W,Henchal EA,Yoosook C

    更新日期：1989-12-01 00:00:00

  • HyBeacon probes: a new tool for DNA sequence detection and allele discrimination.

    abstract：:Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2001.0384

    authors： French DJ,Archard CL,Brown T,McDowell DG

    更新日期：2001-12-01 00:00:00

  • The specificity of pilin DNA sequences for the detection of pathogenic Neisseria.

    abstract：:A nucleic acid hybridization assay for the detection of the pilin gene of Neisseria gonorrhoeae has been devised. The method involves solution hybridization of pilin specific synthetic oligonucleotide probes to genomic DNA in crude cell lysates. This is followed by capture of the probe-target complex onto a microtitre...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(89)90037-6

    authors： Kolberg JA,Besemer DJ,Stempien MM,Urdea MS

    更新日期：1989-03-01 00:00:00

  • Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus.

    abstract：:Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybri...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(89)90026-1

    authors： Sakamoto H,Lemaire O,Merdinoglu D,Guesdon JL

    更新日期：1989-06-01 00:00:00

  • Quantitative PCR evaluation of deletions/duplications identified by array CGH.

    abstract：:Genomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentia...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.101421

    authors： Baldan F,Passon N,Burra S,Demori E,Russo PD,Damante G

    更新日期：2019-08-01 00:00:00

  • Experiences on the application of the polymerase chain reaction in a diagnostic laboratory.

    abstract：:Double polymerase chain reaction (PCR) assays with nested primers have been applied in a routine laboratory for the diagnosis of herpes-, pesti- and retroviral infections of animals. Various methods and tools have been tested to prevent and to eliminate false positive results as well as to visualize the PCR products (...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章,评审

    doi：10.1006/mcpr.1993.1035

    authors： Belák S,Ballagi-Pordány A

    更新日期：1993-06-01 00:00:00

  • CircHIPK3 regulates melanoma cell behaviors by binding with miR-215-5p to upregulate YY1.

    abstract：OBJECT:To investigate the role of circHIPK3 in melanoma. METHODS:Bioinformatics analysis and experiments including RT-qPCR, Pearson's correlation analysis, luciferase reporter, Western blot, and RIP assays were applied to explore the function and mechanism of circHIPK3 in melanoma. RESULTS:CircHIPK3 expression was st...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101644

    authors： Zhu X,Sun J

    更新日期：2020-10-01 00:00:00

  • A novel highly informative polyA microsatellite on the telomeric side of the INK4a/ARF locus.

    abstract：:The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2001.0352

    authors： Chaubert P,Burri N,Cousin P,Shaw P

    更新日期：2001-06-01 00:00:00

  • Development of a TB green II-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine circovirus 2 and 3.

    abstract：:Porcine circovirus 3 (PCV3), as a newly emerged circovirus, is widely distributed in pig populations worldwide. Co-infection of PCV2 and PCV3 has been reported frequently in clinical samples. In the present study, a TB Green II-based duplex real-time polymerase chain reaction (qPCR) was developed to rapidly and differ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.04.001

    authors： Zhao Y,Han HY,Fan L,Tian RB,Cui JT,Li JY,Chen HY,Yang MF,Zheng LL

    更新日期：2019-06-01 00:00:00