Abstract:
:Clostridium septicum is a spore-forming anaerobe frequently implicated in cases of gangrenous dermatitis (GD) and other spontaneously occurring myonecrotic infections of poultry. Although C. septicum is readily cultured from diseased tissues it can be difficult to enumerate due to its tendency to swarm over the surface of agar plates. In this study a quantitative real-time PCR assay was developed in order to more accurately measure the levels of C. septicum in healthy as well as GD associated poultry samples. The assay was specifically designed to target the C. septicum alpha toxin gene, csa, which is, to our knowledge, carried by all strains of C. septicum and has been shown to be essential for virulence. Genomic DNAs from a diverse collection of bacterial species, including closely related Clostridium chauvoei, Clostridium carnis, Clostridium tertium as well as several strains of Clostridium perfringens, all failed to produce a positive reaction. An approximate reproducible limit of detection in spiked extracts of at least 10(3) cfu/g of C. septicum was observed for a variety of different sample types. C. septicum levels in broiler chicken field samples estimated from the results of qPCR were statistically correlated to culture based enumerations obtained from those same tissues.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Neumann AP,Dunham SM,Rehberger TG,Siragusa GRdoi
10.1016/j.mcp.2010.04.001subject
Has Abstractpub_date
2010-08-01 00:00:00pages
211-8issue
4eissn
0890-8508issn
1096-1194pii
S0890-8508(10)00021-6journal_volume
24pub_type
杂志文章abstract::This study evaluates five cryptic plasmid-derived DNA probes in a 4-h slot-blot hybridization assay for the detection of Chlamydia trachomatis in cultures and clinical specimens. The probes, consisting of either the entire cloned 7.5 kbp cryptic plasmid pSE8 or one of four Hin dIII/Eco RI fragments measuring 710, 1055...
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1016/j.mcp.2008.10.001
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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doi:10.1016/j.mcp.2004.06.006
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1006/mcpr.1993.1004
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journal_title:Molecular and cellular probes
pub_type: 杂志文章
doi:10.1006/mcpr.1994.1025
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
pub_type: 杂志文章,评审
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pub_type: 杂志文章,meta分析
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journal_title:Molecular and cellular probes
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abstract::A simple dual-label hybridization test for normal and mutant cystic fibrosis (CF) alleles is described. The assay is based on time-resolved fluorometry (TRF), which allows the simultaneous detection of DNA probes labelled with different lanthanides from one hybridization reaction. DNA was liberated from dried blood di...
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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journal_title:Molecular and cellular probes
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abstract::The human SNAIL is an important developmental protein involved in the formation of mesoderm and neural crest. The protein contains three classic and one atypical zinc-finger motif. The SNAI1 gene is composed of three exons. We have identified three SNPs in non-coding regions, two in the 5'UTR and one in intron 1, whic...
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