Quantitative real-time PCR assay for Clostridium septicum in poultry gangrenous dermatitis associated samples.


:Clostridium septicum is a spore-forming anaerobe frequently implicated in cases of gangrenous dermatitis (GD) and other spontaneously occurring myonecrotic infections of poultry. Although C. septicum is readily cultured from diseased tissues it can be difficult to enumerate due to its tendency to swarm over the surface of agar plates. In this study a quantitative real-time PCR assay was developed in order to more accurately measure the levels of C. septicum in healthy as well as GD associated poultry samples. The assay was specifically designed to target the C. septicum alpha toxin gene, csa, which is, to our knowledge, carried by all strains of C. septicum and has been shown to be essential for virulence. Genomic DNAs from a diverse collection of bacterial species, including closely related Clostridium chauvoei, Clostridium carnis, Clostridium tertium as well as several strains of Clostridium perfringens, all failed to produce a positive reaction. An approximate reproducible limit of detection in spiked extracts of at least 10(3) cfu/g of C. septicum was observed for a variety of different sample types. C. septicum levels in broiler chicken field samples estimated from the results of qPCR were statistically correlated to culture based enumerations obtained from those same tissues.


Mol Cell Probes


Neumann AP,Dunham SM,Rehberger TG,Siragusa GR




Has Abstract


2010-08-01 00:00:00














  • Differentiation of Sarcocystis neurona from eight related coccidia by random amplified polymorphic DNA assay.

    abstract::Four isolates of Sarcocystis neurona from horses with equine protozoal myeloencephalitis and eight species of coccidia from the genera Sarcocystis, Toxoplasma or Eimeria were differentiated using the random amplified polymorphic DNA assay. A single, common, 550-bp DNA fragment was amplified from the DNA of each S. neu...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Granstrom DE,MacPherson JM,Gajadhar AA,Dubey JP,Tramontin R,Stamper S

    更新日期:1994-10-01 00:00:00

  • Characterization of a Caenorhabditis elegans glc seven-like phosphatase (gsp) orthologue from Haemonchus contortus (Nematoda).

    abstract::A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larva...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Campbell BE,Rabelo EM,Hofmann A,Hu M,Gasser RB

    更新日期:2010-08-01 00:00:00

  • High-throughput qualitative multiplex 5' nuclease assay using post-only PCR analysis.

    abstract::Homogenous fluorescence PCR assays offer distinct advantages for qualitative testing and are gaining immense popularity in fields like diagnostic microbiology. To meet the demand of high-volume laboratories, we developed a protocol for qualitative multiplex 5' nuclease assays using post-only PCR analysis. This novel a...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Antonishyn NA,McDonald RR,Chan EL,Horsman GB

    更新日期:2005-10-01 00:00:00

  • Direct identification of Pseudomonas aeruginosa from blood culture bottles by PCR-enzyme linked immunosorbent assay using oprI gene specific primers.

    abstract::A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for direct identification of Pseudomonas aeruginosa from positive BACTEC blood culture bottles. PCR primers were designed to target a 249 bp sequence of the oprI gene in P. aeruginosa. Biotin-labeled probe (PA3) targeted to the species-specific motif we...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Kurupati P,Kumarasinghe G,Laa Poh C

    更新日期:2005-12-01 00:00:00

  • HyBeacon probes: a new tool for DNA sequence detection and allele discrimination.

    abstract::Technologies that permit rapid investigation of DNA sequences, such as those containing single nucleotide polymorphisms (SNPs), are of great consequence to many sectors that perform molecular diagnostic analyses. We have developed a novel fluorescent oligonucleotide probe technology, termed HyBeacons, which provides a...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: French DJ,Archard CL,Brown T,McDowell DG

    更新日期:2001-12-01 00:00:00

  • Evaluation of a DNA probe of plasmid origin for the detection of Chlamydia trachomatis in cultures and clinical specimens.

    abstract::This study evaluates five cryptic plasmid-derived DNA probes in a 4-h slot-blot hybridization assay for the detection of Chlamydia trachomatis in cultures and clinical specimens. The probes, consisting of either the entire cloned 7.5 kbp cryptic plasmid pSE8 or one of four Hin dIII/Eco RI fragments measuring 710, 1055...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Cano RJ,Murrieta CM,Spaulding DC,Pascual A

    更新日期:1991-12-01 00:00:00

  • Specific detection of enterovirus 71 directly from clinical specimens using real-time RT-PCR hybridization probe assay.

    abstract::Enterovirus 71 (EV71) is one of the main causative agents of hand, foot and mouth disease (HFMD) in young children. Infections caused by EV71 could lead to many complications, ranging from brainstem encephalitis to pulmonary oedema, resulting in high mortality. Thus, rapid detection of the virus is required to enable ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Tan EL,Chow VT,Kumarasinghe G,Lin RT,Mackay IM,Sloots TP,Poh CL

    更新日期:2006-04-01 00:00:00

  • Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.

    abstract::Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 's...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Peruzzi B,Serra M,Pescucci C,Sica M,Lastraioli S,Rondelli T,Pedemonte S,Risitano AM,De Angioletti M,Piccioli P,Notaro R

    更新日期:2010-12-01 00:00:00

  • Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples.

    abstract::A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis. ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ren H,Yang M,Zhang G,Liu S,Wang X,Ke Y,Du X,Wang Z,Huang L,Liu C,Chen Z

    更新日期:2016-04-01 00:00:00

  • No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls.

    abstract::New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sabunciyan S,Mandelberg N,Rabkin CS,Yolken R,Viscidi R

    更新日期:2011-04-01 00:00:00

  • New polymorphisms within the variable number tandem repeat (VNTR) 7 locus of Mycobacterium avium subsp. paratuberculosis.

    abstract::Variable number tandem repeat (VNTR) is a frequently employed typing method of Mycobacterium avium paratuberculosis (MAP) isolates. Based on whole genome sequencing in a previous study, allelic diversity at some VNTR loci seems to over- or under-estimate the actual phylogenetic variance among isolates. Interestingly, ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Fawzy A,Zschöck M,Ewers C,Eisenberg T

    更新日期:2016-06-01 00:00:00

  • Screening for cystic fibrosis in dried blood spots of newborns.

    abstract::We propose a newborn cystic fibrosis (CF) screening test based on the analysis of dried blood spot DNA by a strategy involving simple or multiplex denaturing gradient gel electrophoresis (DGGE) of PCR products of CFTR gene fragments, in conjunction with the immunoreactive-trypsin (IRT) assay. From May 1988 to May 1992...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Audrézet MP,Costes B,Ghanem N,Fanen P,Verlingue C,Morin JF,Mercier B,Goossens M,Férec C

    更新日期:1993-12-01 00:00:00

  • PTPN14 acts as a candidate tumor suppressor in prostate cancer and inhibits cell proliferation and invasion through modulating LATS1/YAP signaling.

    abstract::Protein tyrosine phosphatase, non-receptor type 14 (PTPN14) exerts a profound effect in the progression of multiple malignant tumors. However, whether PTPN14 plays a role in prostate cancer has not been well investigated. Herein, we evaluated the function and potential underlying mechanism of PTPN14 in prostate cancer...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wang R,Du Y,Shang J,Dang X,Niu G

    更新日期:2020-10-01 00:00:00

  • Immunoblot and enzyme-linked immunosorbent assays of Campylobacter major outer-membrane protein and application to the differentiation of Campylobacter species.

    abstract::The major outer-membrane protein (MOMP) from Campylobacter jejuni NCTC 11168 was purified by solubilization in Triton X-100. Whole-cell proteins of Campylobacter species and the MOMP were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linke...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Taylor DE,Chang N

    更新日期:1987-09-01 00:00:00

  • Multiplex PCR for avian pathogenic mycoplasmas.

    abstract::Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasm...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wang H,Fadl AA,Khan MI

    更新日期:1997-06-01 00:00:00

  • Development and evaluation of the polymerase chain reaction method for diagnosis of Mycoplasma gallisepticum infection in chickens.

    abstract::A polymerase chain reaction (PCR) method specific for Mycoplasma gallisepticum (MG) was evaluated. The PCR method was found to detect as few as two colour changing units (CCU) of MG and did not give false positive reactions with other avian mycoplasmas. In chickens inoculated with either MG or Mycoplasma synoviae (MS)...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Slavik MF,Wang RF,Cao WW

    更新日期:1993-12-01 00:00:00

  • On-line moisture determination of ore concentrates 'a review of traditional methods and introduction of a novel solution'.

    abstract::The manual gravimetric drying moisture determination methods currently employed by most mineral processing plants fail to provide timely and accurate information required for automatic control. The costs associated with transporting and handling concentrates still represent a major portion of the overall treatment pri...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Cancilla PA,Barrette P,Rosenblum F

    更新日期:2002-12-01 00:00:00

  • CircHIPK3 regulates melanoma cell behaviors by binding with miR-215-5p to upregulate YY1.

    abstract:OBJECT:To investigate the role of circHIPK3 in melanoma. METHODS:Bioinformatics analysis and experiments including RT-qPCR, Pearson's correlation analysis, luciferase reporter, Western blot, and RIP assays were applied to explore the function and mechanism of circHIPK3 in melanoma. RESULTS:CircHIPK3 expression was st...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Zhu X,Sun J

    更新日期:2020-10-01 00:00:00

  • Acquisition of uncharacterized sequences from Candidatus liberibacter, an unculturable bacterium, using an improved genomic walking method.

    abstract::An effective PCR-based genomic walking approach is described to discover previously unknown flanking genomic DNA sequences from Candidatus Liberibacter asiaticus, an unculturable, phloem-limited bacterium. Using this technique, 8564bp of new DNA sequences were obtained from three genomic loci; tufB-secE-nusG-rplKAJL-r...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lin H,Doddapaneni H,Bai X,Yao J,Zhao X,Civerolo EL

    更新日期:2008-02-01 00:00:00

  • Detection of adenovirus in the waters of the Seine River estuary by nested-PCR.

    abstract::Several systems for isolating viruses from environmental samples have been tested. The most promising method is based on genomic amplification. The authors attempted to detect adenovirus in nucleic-acid extracts from the Seine River estuary by a two-step amplification of a 220-bp segment of the conserved coding region...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Castignolles N,Petit F,Mendel I,Simon L,Cattolico L,Buffet-Janvresse C

    更新日期:1998-06-01 00:00:00

  • Construction of an internal control for the detection of Chlamydia pneumoniae by PCR.

    abstract::For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ursi D,Ieven M,Van Bever HP,Goossens H

    更新日期:1998-08-01 00:00:00

  • Use of antibodies against the P36 protein of Mycoplasma hyopneumoniae for the identification of M. hyopneumoniae strains.

    abstract::Mycoplasma hyopneumoniae, the principal aetiological agent of porcine enzootic pneumonia, synthesizes a 36 kDa protein (P36) which is an early and strong immunogenic factor in experimentally and naturally infected swine. Polyclonal antibodies were made against the recombinant P36 protein in rabbits and used for the id...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Stipkovits L,Nicolet J,Haldimann A,Frey J

    更新日期:1991-12-01 00:00:00

  • Genetic differentiation and phylogeny of 27 sheep populations based on structural gene loci.

    abstract::To explore the genetic divergence and phylogeny of Chinese indigenous sheep breeds, in the current study, we analyzed the polymorphisms of 5 structural loci in ten sheep populations, including Sishui Fur, Sunite, Wurank, Bayinbuluke, Altay, Small-Tailed Han, Wadi, Tan, Tong and Hu sheep. The data were then compared wi...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Li M,Xia H,Chen D,Ji D,Kenji T,Li R,Liao X,Mao Y,Sun W,Geng R,Yang Z

    更新日期:2018-02-01 00:00:00

  • Detection of Pseudomonas aeruginosa in sputum from cystic fibrosis patients by the polymerase chain reaction.

    abstract::A DNA amplification procedure using heat stable Taq polymerase and the polymerase chain reaction is described for the detection of Pseudomonas aeruginosa in specimens from cystic fibrosis patients. A set of primers was selected on the basis of the nucleotide sequence of the algD gene encoding GDP mannose dehydrogenase...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: McIntosh I,Govan JR,Brock DJ

    更新日期:1992-08-01 00:00:00

  • Human metapneumovirus uses endocytosis pathway for host cell entry.

    abstract::Human metapneumovirus (hMPV) is a prevalent pathogen worldwide and causes various respiratory infections. Although it is a critical pathogen in pediatric patients, it is unclear how it enters host cells. In this study, we focused on hMPV cell entry using two kinds of cell lines (Vero E6 and LLC-MK2), which are most co...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yang H,He H,Tan B,Liu E,Zhao X,Zhao Y

    更新日期:2016-08-01 00:00:00

  • Development of a novel internal positive control for Taqman based assays.

    abstract::Development of rapid amplification assays for the detection and identification of biological threat agents has become a focus of diagnostic efforts in recent years. The use of real-time PCR assays as diagnostic tools depends upon two critical processes. First, nucleic acid purification must provide template that is bo...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hartman LJ,Coyne SR,Norwood DA

    更新日期:2005-02-01 00:00:00

  • A novel RT-PCR approach for detection and characterization of citrus viroids.

    abstract::Citrus plants are natural hosts of five viroid species and a large number of sequence variants. Because of their small size, viroids lend themselves to various RT-PCR approaches for their detection and further characterization. The one-step RT-PCR approach proposed here is based on the synthesis of viroid-cDNA by reve...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bernad L,Duran-Vila N

    更新日期:2006-04-01 00:00:00

  • Polymorphism rs3737787 of Upstream Stimulatory Factor 1 gene is associated with serum lipid phenotype in Nigerian population.

    abstract::Serum lipid profile which is determined by genotype-phenotype relationship plays a significant role in the development of cardiovascular disease. Upstream stimulatory factor 1 (USF1), has been reported to be associated with serum lipid levels in different population, hence, this study investigated the association of v...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Onadeko OT,Okunowo WO,Imaga NOA,Abdulrazaq MM,Onuminya OJ,Van-Lare TO,Nwosu M

    更新日期:2020-12-08 00:00:00

  • Evaluation of an alkaline phosphatase-labeled oligonucleotide probe for detection and enumeration of vibrio spp. from shrimp hatchery environment.

    abstract::An alkaline phosphatase (AP)-labeled genus-specific oligonucleotide probe was developed to detect and enumerate vibrios in shrimp larvae and their surrounding environment. The probe was evaluated using 35 laboratory isolates of Vibrio species and 29 isolates of non-vibrio species. The probe was specific for the Vibrio...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Raghunath P,Karunasagar I,Karunasagar I

    更新日期:2007-08-01 00:00:00

  • Detection of Mycobacterium avium subspecies paratuberculosis genetic components in retail cheese curds purchased in Wisconsin and Minnesota by PCR.

    abstract::Research has been focused on the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in pasteurized milk; however, pasteurized milk is a key ingredient in a variety of food products. Therefore, MAP contamination in milk-derived products must be investigated. We undertook a six-month study to investigate...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Clark DL Jr,Anderson JL,Koziczkowski JJ,Ellingson JL

    更新日期:2006-06-01 00:00:00