当前位置： SCI文献检索 > MOLECULAR AND CELLULAR PROBES期刊下所有文献 > Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues.

Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues.

Abstract：

:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-reaction of IBV RT-LAMP was found when tested with other viruses including Newcastle disease virus (NDV), avian reovirus (ARV), and infectious laryngotrachietis virus (ILTV) due to their mismatch with IBV RT-LAMP primers. A total of 187 clinical tissues samples (88 blood, 62 kidney and 37 lung) were evaluated and compared to conventional RT-PCR. The sensitivity of RT-LAMP and RT-PCR assays for detecting IBV RNA in clinical specimens was 99.5% and 98.4%, respectively. These findings showed that the RT-LAMP assay has potential usefulness for rapid and sensitive diagnosis in outbreak of IBV.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Chen HT,Zhang J,Ma YP,Ma LN,Ding YZ,Liu XT,Cai XP,Ma LQ,Zhang YG,Liu YS

doi

10.1016/j.mcp.2009.10.001

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

104-6

issue

2

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(09)00064-4

journal_volume

24

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR PROBES文献大全
  • Rapid and cost effective genotyping method for polymorphisms in PPARG, PPARGC1 and TCF7L2 genes.

    abstract：:Polymorphisms (rs1801282, rs8192678, rs7903146) of peroxisome proliferator-activated receptor gamma (PPARG), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PPARGC1A) and transcription factor 7-like 2 (TCF7L2) have recently been associated with different diseases, mainly type 2 diabetes. An assay...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2008.10.001

    authors： Habalová V,Klimcáková L,Zidzik J,Tkác I

    更新日期：2009-02-01 00:00:00

  • A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR.

    abstract：:Species identification of Mycobacterium tuberculosis remains a cumbersome process. We have developed a simple method for treating clinical samples which permits direct polymerase chain reaction (PCR) amplification of mycobacterial target DNA without organic extraction. Samples were boiled for 30 min in TE-Triton, then...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(06)80011-3

    authors： Sritharan V,Barker RH Jr

    更新日期：1991-10-01 00:00:00

  • Impact of fixation artifacts and threshold selection on high resolution melting analysis for KRAS mutation screening.

    abstract：INTRODUCTION:Treatment in metastatic colorectal cancer (mCRC) has expanded with monoclonal antibodies targeting epidermal growth factor receptor, but is restricted to patients with a wild-type (WT) KRAS mutational status. The most sensitive assays for KRAS mutation detection in formalin-fixed paraffin embedded (FFPE) t...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2017.06.003

    authors： Pérez-Báez W,García-Latorre EA,Maldonado-Martínez HA,Coronado-Martínez I,Flores-García L,Taja-Chayeb L

    更新日期：2017-10-01 00:00:00

  • A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter.

    abstract：:In a previous study, we reported the performance of a PCR assay amplifying 287-bp of the 16S rRNA gene of thermo-tolerant Campylobacter (C. jejuni, C. lari, C. coli) through an international ring-trial involving 12 participating laboratories. Based on the validated set of primers, a LightCycler real-time PCR assay (LC...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2004.04.005

    authors： Perelle S,Josefsen M,Hoorfar J,Dilasser F,Grout J,Fach P

    更新日期：2004-10-01 00:00:00

  • Detection of Pseudomonas aeruginosa in sputum from cystic fibrosis patients by the polymerase chain reaction.

    abstract：:A DNA amplification procedure using heat stable Taq polymerase and the polymerase chain reaction is described for the detection of Pseudomonas aeruginosa in specimens from cystic fibrosis patients. A set of primers was selected on the basis of the nucleotide sequence of the algD gene encoding GDP mannose dehydrogenase...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(92)90005-i

    authors： McIntosh I,Govan JR,Brock DJ

    更新日期：1992-08-01 00:00:00

  • miR-410-5p promotes the development of diabetic cardiomyopathy by suppressing PIM1-induced anti-apoptosis.

    abstract：:Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myoc...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101558

    authors： Xia X,Liang Y,Zheng W,Lin D,Sun S

    更新日期：2020-08-01 00:00:00

  • Potential of recombinant flagellin fragment from Burkholderia thailandensis as an antigen for melioidosis antibody detection by indirect ELISA.

    abstract：:Non-pathogenic Burkholderia thailandensis may be used as a model for Burkholderia pseudomallei due to the genetic similarity of these species. Moreover, the experimental manipulation of B. thailandensis is safer. In this study, we constructed recombinant flagellin protein fragments of B. thailandensis E264 (FLAG300, F...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2012.11.001

    authors： Wajanarogana S,Nimnuch P,Thongmee A,Kritsiriwuthinan K

    更新日期：2013-04-01 00:00:00

  • Amplification of three hypervariable DNA regions by polymerase chain reaction for paternity determinations: comparison with conventional methods and DNA fingerprinting.

    abstract：:The present study evaluates the usefulness of a PCR-based method for routine paternity testing in 35 paternity cases. This identification method which is based on amplification of three hypervariable genetic loci, apoB, D1S80 and HLA-DQ alpha, is compared, with regard to reliability and technical feasibility, to the c...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(92)90067-8

    authors： Helminen P,Sajantila A,Johnsson V,Lukka M,Ehnholm C,Peltonen L

    更新日期：1992-02-01 00:00:00

  • DNA probe and polymerase chain reaction procedure for the specific detection of Serpulina hyodysenteriae.

    abstract：:Serpulina (Treponema) hyodysenteriae, a Gram-negative anaerobic spirochete, is the causative agent of swine dysentery, a mucohaemorrhagic diarrheal disease in which lesions are confined to the large intestine of pigs. A DNA probe and polymerase chain reaction (PCR) amplification procedures which are specific, rapid , ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(95)80035-2

    authors： Harel J,Forget C

    更新日期：1995-04-01 00:00:00

  • Direct extraction and molecular characterization of enteroviruses genomes from human faecal samples.

    abstract：:Routine diagnosis of acute flaccid paralysis (AFP) is still based on classical virological procedures. Several enteroviruses serotypes are not easily isolated in cell cultures system used and routinely more than one passage in cell culture is performed. A total of 54 archived faecal samples were examined. The heteroge...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2007.12.001

    authors： Bolanaki E,Kottaridi C,Dedepsidis E,Kyriakopoulou Z,Pliaka V,Pratti A,Levidiotou-Stefanou S,Markoulatos P

    更新日期：2008-06-01 00:00:00

  • Allelic variation in the promoter region of the LDL receptor gene: analysis of an African-specific variant in the FP2 cis-acting regulatory element.

    abstract：:DNA samples of 2303 individuals from nine different population groups were screened for variant -175g-->t in the promoter region of the low-density lipoprotein receptor (LDLR) gene. The -175g-->t variant detected at carrier frequencies of 3-10% in different African population groups was absent in the Caucasian and Asi...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(03)00050-1

    authors： Hoogendijk CF,Scholtz CL,Pimstone SM,Ehrenborg E,Kastelein JJ,Defesche JC,Thiart R,du Plessis L,de Villiers JN,Zaahl MG,Delport R,Rubinsztein DC,Raffel LJ,Grim CE,Mediene-Benchekor S,Amouyel P,Brousseau T,Steyn K,Lomb

    更新日期：2003-08-01 00:00:00

  • Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

    abstract：:Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1994.1035

    authors： Axton RA,Brock DJ

    更新日期：1994-06-01 00:00:00

  • The specificity of pilin DNA sequences for the detection of pathogenic Neisseria.

    abstract：:A nucleic acid hybridization assay for the detection of the pilin gene of Neisseria gonorrhoeae has been devised. The method involves solution hybridization of pilin specific synthetic oligonucleotide probes to genomic DNA in crude cell lysates. This is followed by capture of the probe-target complex onto a microtitre...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(89)90037-6

    authors： Kolberg JA,Besemer DJ,Stempien MM,Urdea MS

    更新日期：1989-03-01 00:00:00

  • Screening for cystic fibrosis in dried blood spots of newborns.

    abstract：:We propose a newborn cystic fibrosis (CF) screening test based on the analysis of dried blood spot DNA by a strategy involving simple or multiplex denaturing gradient gel electrophoresis (DGGE) of PCR products of CFTR gene fragments, in conjunction with the immunoreactive-trypsin (IRT) assay. From May 1988 to May 1992...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1993.1073

    authors： Audrézet MP,Costes B,Ghanem N,Fanen P,Verlingue C,Morin JF,Mercier B,Goossens M,Férec C

    更新日期：1993-12-01 00:00:00

  • A novel highly informative polyA microsatellite on the telomeric side of the INK4a/ARF locus.

    abstract：:The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2001.0352

    authors： Chaubert P,Burri N,Cousin P,Shaw P

    更新日期：2001-06-01 00:00:00

  • High-throughput qualitative multiplex 5' nuclease assay using post-only PCR analysis.

    abstract：:Homogenous fluorescence PCR assays offer distinct advantages for qualitative testing and are gaining immense popularity in fields like diagnostic microbiology. To meet the demand of high-volume laboratories, we developed a protocol for qualitative multiplex 5' nuclease assays using post-only PCR analysis. This novel a...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2005.06.001

    authors： Antonishyn NA,McDonald RR,Chan EL,Horsman GB

    更新日期：2005-10-01 00:00:00

  • LncRNA GAS5 participates in the regulation of dexamethasone on androgen receptor -negative and -positive prostate cancer cell proliferation.

    abstract：BACKGROUND:Androgen receptor (AR) and long non-coding RNAs (lncRNA) play important roles in the initiation and progression of prostate cancer (PCa). The present study was designed to investigate whether lncRNA growth arrest-specific 5 (GAS5) is involved in the regulation of dexamethasone on the proliferation of AR+ PCa...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101607

    authors： Hu J,Deng J,Cao R,Xiong S,Guo J

    更新日期：2020-10-01 00:00:00

  • Use of DNA restriction endonuclease digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from human and animal hosts.

    abstract：:Variation amongst strains of Helicobacter pylori and Helicobacter mustelae was examined by DNA restriction endonuclease digestion and rRNA gene patterns generated using a non-radioactive probe. Chromosomal DNA was extracted from 30 cultures of H. pylori from human, Rhesus monkey and pig gastric mucosa, and from three ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(90)90023-s

    authors： Morgan DD,Owen RJ

    更新日期：1990-08-01 00:00:00

  • Quantitative PCR for the diagnosis of cutaneous leishmaniasis from formalin-fixed and paraffin-embedded skin sections.

    abstract：:The present report describes a real-time PCR-based procedure to reliably determine the quantity of Leishmania amastigotes in relation to the amount of host tissue in histological skin sections from canine and equine cases of cutaneous leishmaniasis. The novel diagnostic Leishmania-PCR has a detection limit of <0.02 am...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2015.09.008

    authors： Müller N,Hentrich B,Frey CF,Welle M

    更新日期：2015-12-01 00:00:00

  • Taxonomic and phylogenetic status of non-tuberculous mycobacteria in a Caribbean setting.

    abstract：:This report describes detailed taxonomic and phylogenetic analysis of 15 non-tuberculous mycobacteria (NTMs) isolated from human pathological specimens in a Caribbean setting (12 slow-growers and three rapid-growers) that were not identified by cultural and biochemical tests and drug-susceptibility results. These isol...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2004.06.006

    authors： Ferdinand S,Legrand E,Goh KS,Berchel M,Mazzarelli G,Sola C,Tortoli E,Rastogi N

    更新日期：2004-12-01 00:00:00

  • A novel RT-PCR approach for detection and characterization of citrus viroids.

    abstract：:Citrus plants are natural hosts of five viroid species and a large number of sequence variants. Because of their small size, viroids lend themselves to various RT-PCR approaches for their detection and further characterization. The one-step RT-PCR approach proposed here is based on the synthesis of viroid-cDNA by reve...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2005.11.001

    authors： Bernad L,Duran-Vila N

    更新日期：2006-04-01 00:00:00

  • Cloning of the antigen 85A from Mycobacterium gordonae and its use for the specific PCR identification of these mycobacteria.

    abstract：:The complete nucleotide sequence of 85A antigen of Mycobacterium gordonae was determined. This gene encodes 339 amino acids, including 43 amino acids for the signal peptide, followed by a mature protein of 296 amino acids. A polymerase chain reaction (PCR) assay for the rapid detection of M. gordonae DNA using two pai...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1997.0110

    authors： Dumonceaux M,Fauville Dufaux M,Ooms J,De Wit L,Sonck P,Content J

    更新日期：1997-08-01 00:00:00

  • Serogroup specific single and multiplex PCR with pre-enrichment culture and immuno-magnetic bead capture for identifying strains of D. nodosus in sheep with footrot prior to vaccination.

    abstract：:The identification of Dichelobacter nodosus present in a flock is a prerequisite to specific (autogenous) vaccination. Current methods of identification of the serogroup present in a population requires that the organisms be isolated, identified visually in mixed culture on streak plates, subcultured to purify and sub...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2002.0427

    authors： Dhungyel OP,Whittington RJ,Egerton JR

    更新日期：2002-08-01 00:00:00

  • Simultaneous detection of erythromycin-resistant methylase genes ermA and ermC from Staphylococcus spp. by multiplex-PCR.

    abstract：:A comparative analysis of the two most dominant erythromycin-resistance determinant genes in Staphylococcus sppnamely, the ermA and ermC genes, was carried out. Sixty erythromycin-resistant strains of Staphylococcus spp. were tested, of which 24 were avian and 36 were clinical isolates. Our results indicated the preva...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1999.0265

    authors： Khan SA,Nawaz MS,Khan AA,Cerniglia CE

    更新日期：1999-10-01 00:00:00

  • Detection of shigellae, enteroinvasive and enterotoxigenic Escherichia coli using the polymerase chain reaction (PCR) in patients returning from tropical countries.

    abstract：:We have used the polymerase chain reaction (PCR) to detect shigellae, EIEC and ETEC in stool specimens of diarrhoeic patients returning from tropical countries. As compared to culture (7.1% positive specimens), which recognizes only Shigella strains, PCR performed on bacterial growth from directly inoculated MacConkey...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1994.1040

    authors： Lüscher D,Altwegg M

    更新日期：1994-08-01 00:00:00

  • Direct identification of Pseudomonas aeruginosa from blood culture bottles by PCR-enzyme linked immunosorbent assay using oprI gene specific primers.

    abstract：:A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for direct identification of Pseudomonas aeruginosa from positive BACTEC blood culture bottles. PCR primers were designed to target a 249 bp sequence of the oprI gene in P. aeruginosa. Biotin-labeled probe (PA3) targeted to the species-specific motif we...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2005.07.005

    authors： Kurupati P,Kumarasinghe G,Laa Poh C

    更新日期：2005-12-01 00:00:00

  • Diagnosis of mouse hepatitis virus contamination in mouse population by using nude mice and RT-PCR.

    abstract：:Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficult. Reverse transcription plus polymerase chain reaction (RT-PCR) was ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1998.0211

    authors： Wang RF,Campbell WL,Cao WW,Colvert RM,Holland MA,Cerniglia CE

    更新日期：1999-02-01 00:00:00

  • Up-regulated miR-374a-3p relieves lipopolysaccharides induced injury in CHON-001 cells via regulating Wingless-type MMTV integration site family member 5B.

    abstract：BACKGROUND:Osteoarthritis (OA) is a frequent and incurable joint disease, inducing significant pain and seriously threatening to human health. It has been reported that microRNAs (miRNAs) play crucial roles on cancers and inflammatory diseases via cooperating with genes. However, the effect of miR-374a-3p/Wingless-type...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101541

    authors： Shi FL,Ren LX

    更新日期：2020-06-01 00:00:00

  • A fluorescent method for detecting low-grade 11patUPD mosaicism in Beckwith-Wiedemann syndrome.

    abstract：:The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2003.07.002

    authors： Russo S,Mencarelli M,Cavalleri F,Selicorni A,Cogliati F,Larizza L

    更新日期：2003-12-01 00:00:00

  • Multiple paternity and sperm storage in captive Hermann's tortoises, Testudo hermanni boettgeri determined from amniotic fluid adhering to the eggshell.

    abstract：:We identified multiple paternity in 52.9% of the clutches of Hermann's tortoise Testudo hermanni boettgeri using polymorphic microsatellite markers. In addition we demonstrated sperm storage across seasons. DNA was extracted from the amniotic fluid adhering to the eggshell's inner surface, a procedure suitable for eas...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2015.05.009

    authors： Farke CM,Olek K,Gerding WM,Distler C

    更新日期：2015-08-01 00:00:00