Detection of Pseudomonas aeruginosa in sputum from cystic fibrosis patients by the polymerase chain reaction.

abstract：:Quantification of nucleic acids, especially of mRNA, is increasingly important in biomedical research. The recently developed quantitative real-time polymerase chain reaction (PCR) - a highly sensitive technology for the rapid, accurate and reproducible quantification of gene expression - offers major advantages over ...

journal_title：Molecular and cellular probes

authors： Fronhoffs S,Totzke G,Stier S,Wernert N,Rothe M,Brüning T,Koch B,Sachinidis A,Vetter H,Ko Y

更新日期：2002-04-01 00:00:00

abstract：:A polymorphic GT dinucleotide repeat sequence has been identified in the 5' flanking region of the human growth hormone receptor (hGHR) gene on chromosome 5p13.1-p12, within the promoter region of the V9 5'UTR exon. Thirteen alleles have been identified in 50 non-related individuals, with an observed heterozygosity of...

journal_title：Molecular and cellular probes

authors： Hadjiyannakis S,Zheng H,Hendy GN,Goodyer CG

更新日期：2001-08-01 00:00:00

abstract：:The ligase detection reaction (LDR) is a highly specific genotyping method for single nucleotide variations. Although LDR typically discriminates single nucleotide polymorphism (SNP) alleles at the 3' end of so-called LDR discriminating probes, we designed probes in which the position of nucleotide differences for dis...

journal_title：Molecular and cellular probes

authors： Asari M,Omura T,Maseda C,Shiono H,Tasaki Y,Matsubara K,Shimizu K

更新日期：2010-12-01 00:00:00

abstract：:Multiplex ligation dependent probe amplification (MLPA) assays were designed for the genes HEXB (OMIM: 606873), GM2A (OMIM: 613109) and SMARCAL1 (OMIM: 606622) of humans. Two sets of synthetic MLPA probes for these coding exons were tested. Changes in copy numbers were detected as well as single nucleotide polymorphis...

journal_title：Molecular and cellular probes

authors： Sobek AK,Evers C,Dekomien G

更新日期：2013-02-01 00:00:00

abstract：:Many highly homologous genes are present in the murine major histocompatibility complex (MHC) class I gene family. Consequently, it is difficult to distinguish between RNA transcripts of individual class I genes solely on the basis of nucleic acid hybridization analysis using DNA probes over 50 base pairs long. To avo...

journal_title：Molecular and cellular probes

authors： Mellor AL

更新日期：1987-09-01 00:00:00

abstract：:Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasm...

journal_title：Molecular and cellular probes

authors： Wang H,Fadl AA,Khan MI

更新日期：1997-06-01 00:00:00

abstract：:A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments perf...

journal_title：Molecular and cellular probes

authors： Thaller MC,Berlutti F,Riccio ML,Rossolini GM

更新日期：1992-10-01 00:00:00

abstract：:The pyrazinamidase gene coding for the enzyme that activates the bactericidal drug pyrazinamide contains a polymorphic site that is preserved in Mycobacterium bovis. We synthesized two sets of primers, one encompassing a 180 bp fragment, and the second spanning a 726 bp fragment including the full pncA gene. Following...

journal_title：Molecular and cellular probes

authors： Barouni AS,Augusto CJ,Lopes MT,Zanini MS,Salas CE

更新日期：2004-06-01 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) to detect shigellae, EIEC and ETEC in stool specimens of diarrhoeic patients returning from tropical countries. As compared to culture (7.1% positive specimens), which recognizes only Shigella strains, PCR performed on bacterial growth from directly inoculated MacConkey...

journal_title：Molecular and cellular probes

authors： Lüscher D,Altwegg M

更新日期：1994-08-01 00:00:00

abstract：:A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for direct identification of Pseudomonas aeruginosa from positive BACTEC blood culture bottles. PCR primers were designed to target a 249 bp sequence of the oprI gene in P. aeruginosa. Biotin-labeled probe (PA3) targeted to the species-specific motif we...

journal_title：Molecular and cellular probes

authors： Kurupati P,Kumarasinghe G,Laa Poh C

更新日期：2005-12-01 00:00:00

abstract：:Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA L...

journal_title：Molecular and cellular probes

authors： Yang Y,Qin X,Zhang W,Li Z,Zhang S,Li Y,Zhang Z

更新日期：2017-06-01 00:00:00

abstract：:Natalizumab is a humanized monoclonal antibody against the alpha4 chain of the alpha4beta1 and alpha4beta7 integrin heterodimers used with high effectiveness in the treatment of multiple sclerosis. The use of this drug can unfortunately be associated with the onset of progressive multifocal leukoencephalopathy, a poss...

journal_title：Molecular and cellular probes

authors： Rossi F,Newsome SD,Viscidi R

更新日期：2015-02-01 00:00:00

abstract：:Advanced reflectance-based optical techniques for in vivo imaging often suffer from low contrast between neoplastic and normal tissue and are unable to image early biomolecular changes associated with carcinogenesis, thus limiting their clinical value. In this study, we exploit the resonance light scattering property ...

journal_title：Molecular and cellular probes

authors： Kah JC,Olivo MC,Lee CG,Sheppard CJ

更新日期：2008-02-01 00:00:00

abstract：:Inexpensive fiberglass mesh window screens were used as spacers between colony blot filters to increase the number of bacterial isolates that could be tested by DNA colony hybridization. Sixty filters with up to 48 isolates per filter were tested at one time. This modified technique reduced the amount of radiolabelled...

journal_title：Molecular and cellular probes

authors： Kaysner CA,Weagant SD,Hill WE

更新日期：1988-12-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a prevalent pathogen worldwide and causes various respiratory infections. Although it is a critical pathogen in pediatric patients, it is unclear how it enters host cells. In this study, we focused on hMPV cell entry using two kinds of cell lines (Vero E6 and LLC-MK2), which are most co...

journal_title：Molecular and cellular probes

authors： Yang H,He H,Tan B,Liu E,Zhao X,Zhao Y

更新日期：2016-08-01 00:00:00

abstract：:Holstein haplotype (HH) 1, 3 and 4 are lethal mutations, responsible for early embryonic losses in Holstein Friesian (HF) cattle, worldwide. Three PCR based assays - tetra Amplification Refractory Mutation System PCR, PCR primer induced restriction analysis and PCR-restriction fragment length polymorphism techniques f...

journal_title：Molecular and cellular probes

authors： Kumar A,Gupta ID,Mohan G,Vineeth MR,Ravi Kumar D,Jayakumar S,Niranjan SK

更新日期：2020-04-01 00:00:00

abstract：BACKGROUND:Osteoarthritis (OA) is a frequent and incurable joint disease, inducing significant pain and seriously threatening to human health. It has been reported that microRNAs (miRNAs) play crucial roles on cancers and inflammatory diseases via cooperating with genes. However, the effect of miR-374a-3p/Wingless-type...

journal_title：Molecular and cellular probes

authors： Shi FL,Ren LX

更新日期：2020-06-01 00:00:00

abstract：:We have examined the relationship between Chlamydia trachomatis found in clinical samples in which the cryptic plasmid was absent and known serovars of C. trachomatis. PCR and RNase protection assays were used to compare 12 C. trachomatis serovars and a plasmidless L2 serovar strain with the reactivity of clinical spe...

journal_title：Molecular and cellular probes

authors： An Q,Olive DM

更新日期：1994-10-01 00:00:00

abstract：:The detection limits of the polymerase chain reaction (PCR) for Mycoplasma pneumoniae were determined using specimens from persons known to have had M. pneumoniae pneumonia. Four primers were selected from the known sequence of the P1 gene. The primer pair (P1-178 and P1-809) which generates a 631 fragment gave the lo...

journal_title：Molecular and cellular probes

authors： Leng Z,Kenny GE,Roberts MC

更新日期：1994-04-01 00:00:00

abstract：:Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten b...

journal_title：Molecular and cellular probes

authors： Liu CY,Song HQ,Zhang RL,Chen MX,Xu MJ,Ai L,Chen XG,Zhan XM,Liang SH,Yuan ZG,Lin RQ,Zhu XQ

更新日期：2011-08-01 00:00:00

abstract：:Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficult. Reverse transcription plus polymerase chain reaction (RT-PCR) was ...

journal_title：Molecular and cellular probes

authors： Wang RF,Campbell WL,Cao WW,Colvert RM,Holland MA,Cerniglia CE

更新日期：1999-02-01 00:00:00

abstract：:The polymerase spiral reaction (PSR), a novel isothermal method for targeted DNA amplification, was effectively applied to detect Salmonella in artificially spiked pork. The specificity of the developed PSR was tested using 16 Salmonella and 15 non-Salmonella strains. The PSR assay was 10-fold more sensitive than conv...

journal_title：Molecular and cellular probes

authors： Momin KM,Milton AAP,Ghatak S,Thomas SC,Priya GB,Das S,Shakuntala I,Sanjukta R,Puro KU,Sen A

更新日期：2020-04-01 00:00:00

abstract：:Non-small-cell lung carcinoma (NSCLC) accounts for approximately 80% of lung cancers with a high metastatic potential. Elucidating the mechanism of NSCLC metastasis will provide new promising targets for NSCLC therapy and benefit its prognosis. Plasmacytoma variant translocation 1 (PVT1) has been proven to be overexpr...

journal_title：Molecular and cellular probes

authors： Su XH,Zhu YR,Hou YJ,Li K,Dong NH

更新日期：2020-12-01 00:00:00

abstract：:A nucleic acid hybridization assay for the detection of the pilin gene of Neisseria gonorrhoeae has been devised. The method involves solution hybridization of pilin specific synthetic oligonucleotide probes to genomic DNA in crude cell lysates. This is followed by capture of the probe-target complex onto a microtitre...

journal_title：Molecular and cellular probes

authors： Kolberg JA,Besemer DJ,Stempien MM,Urdea MS

更新日期：1989-03-01 00:00:00

abstract：:Spinal muscular atrophy (SMA), the leading genetic cause of death in childhood, is an autosomal recessive neuromuscular disorder characterized by progressive muscle weakness, associated with deletions of the survival motor neuron 1 (SMN1) gene. Approximately 94% of SMA patients carry homologous deletions of SMN1 exon(...

journal_title：Molecular and cellular probes

authors： Baris I,Etlik O,Koksal V,Arican-Baris ST

更新日期：2010-06-01 00:00:00

abstract：:We have developed and evaluated an ELISA-based detection method for PCR-amplified HIV-1 DNA. The assay uses two oligonucleotide probes which are end-labelled at the 5'-end with biotin or digoxigenin, respectively. Upon solution hybridization of these probes which react with the same strand of amplified DNA product, th...

journal_title：Molecular and cellular probes

authors： Böni J,Schüpbach J

更新日期：1993-10-01 00:00:00

abstract：:DNA samples of 2303 individuals from nine different population groups were screened for variant -175g-->t in the promoter region of the low-density lipoprotein receptor (LDLR) gene. The -175g-->t variant detected at carrier frequencies of 3-10% in different African population groups was absent in the Caucasian and Asi...

journal_title：Molecular and cellular probes

authors： Hoogendijk CF,Scholtz CL,Pimstone SM,Ehrenborg E,Kastelein JJ,Defesche JC,Thiart R,du Plessis L,de Villiers JN,Zaahl MG,Delport R,Rubinsztein DC,Raffel LJ,Grim CE,Mediene-Benchekor S,Amouyel P,Brousseau T,Steyn K,Lomb

更新日期：2003-08-01 00:00:00

abstract：:DNA probes were used to examine tetracycline-resistant Gram-negative bacteria (281 strains representing eight species) from catfish ponds. The isolates, which did not previously hybridize with the Tet A, B and C determinants, were examined for the presence of tetracycline-resistance Tet D and Tet E determinants. The d...

journal_title：Molecular and cellular probes

authors： DePaola A,Roberts MC

更新日期：1995-10-01 00:00:00

abstract：:A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis. ...

journal_title：Molecular and cellular probes

authors： Ren H,Yang M,Zhang G,Liu S,Wang X,Ke Y,Du X,Wang Z,Huang L,Liu C,Chen Z

更新日期：2016-04-01 00:00:00

abstract：PURPOSE:This study aimed to analyze the relationships of long non-coding RNAs (lncRNAs) and protein-coding genes in lung squamous cell carcinoma (LUSC). METHODS:RNA-seq data of LUSC deposited in the TCGA database were used to identify differentially expressed protein-coding genes (DECGs) and differentially expressed l...

journal_title：Molecular and cellular probes

authors： Wu X,Ruan L,Yang Y,Mei Q

更新日期：2016-06-01 00:00:00