Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

Abstract:

:Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA are stable to electrophoresis and show a 1000-fold enhancement after binding. We demonstrate that this reagent can be successfully used in combination with a laser-excited fluorescence gel scanner to detect the three most common cystic fibrosis mutant alleles, delta F508, G551D and G542X. This has potential application to mass population heterozygote screening.

journal_name

Mol Cell Probes

authors

Axton RA,Brock DJ

doi

10.1006/mcpr.1994.1035

subject

Has Abstract

pub_date

1994-06-01 00:00:00

pages

245-50

issue

3

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(84)71035-8

journal_volume

8

pub_type

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