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Rapid identification of Renibacterium salmoninarum using an oligonucleotide probe complementary to 16S rRNA.

Abstract：

:Bacterial kidney disease in salmonid fish is caused by the slow-growing Gram-positive rod, Renibacterium salmoninarum. The partial sequence of 16S rRNA from R. salmoninarum was determined and compared with published bacterial 16S rRNA sequences. From this sequence information, a 30-bases-long oligonucleotide was designed and used as a specific probe for identification of R. salmoninarum in filter hybridization experiments. Strong specific hybridization signals were observed for all strains of R. salmoninarum tested. Furthermore, no cross-hybridization could be seen against 22 other bacterial species, among them other salmonid fish pathogens. The detection limit for the probe in direct filter hybridization by the dot-blot technique was 2.5 x 10(4) bacteria. It was also possible to detect R. salmoninarum in clinical samples by direct filter hybridization.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Mattsson JG,Gersdorf H,Jansson E,Hongslo T,Göbel UB,Johansson KE

doi

10.1006/mcpr.1993.1004

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

25-33

issue

1

eissn

0890-8508

issn

1096-1194

pii

S0890850883710042

journal_volume

7

pub_type

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