Abstract:
:Rapid detection of single-base changes is fundamental to molecular medicine. PCR amplification of specific alleles (PASA) has previously been used as a rapid method of genotyping single-nucleotide changes, but one reaction is required for each allele. This paper describes a Bidirectional PASA (Bi-PASA) method, which was developed to distinguish between homozygotes and heterozygotes in one PCR reaction. The method is tested using the Sp1 polymorphism in Collagen type Ialpha1. The results demonstrate that Bi-PASA is a simple and rapid method for detecting the zygosity of the polymorphism in a single PCR reaction.
journal_name
Mol Cell Probesjournal_title
Molecular and cellular probesauthors
Lundberg GAdoi
10.1016/j.mcp.2006.10.007subject
Has Abstractpub_date
2007-06-01 00:00:00pages
239-41issue
3eissn
0890-8508issn
1096-1194pii
S0890-8508(06)00076-4journal_volume
21pub_type
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