A rapid one-tube PCR method for simultaneously differentiating homozygotes and heterozygotes of the Sp1 binding site polymorphism in collagen type Ialpha1.

Abstract:

:Rapid detection of single-base changes is fundamental to molecular medicine. PCR amplification of specific alleles (PASA) has previously been used as a rapid method of genotyping single-nucleotide changes, but one reaction is required for each allele. This paper describes a Bidirectional PASA (Bi-PASA) method, which was developed to distinguish between homozygotes and heterozygotes in one PCR reaction. The method is tested using the Sp1 polymorphism in Collagen type Ialpha1. The results demonstrate that Bi-PASA is a simple and rapid method for detecting the zygosity of the polymorphism in a single PCR reaction.

journal_name

Mol Cell Probes

authors

Lundberg GA

doi

10.1016/j.mcp.2006.10.007

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

239-41

issue

3

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(06)00076-4

journal_volume

21

pub_type

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