Genetic variation of the methylenetetrahydrofolate reductase and cystathionine beta-synthase genes in Korean patients with coronary artery disease and a new polymorphism in intron 7.


:We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant differences were found in allele frequencies for either gene between the CAD and normal groups although the frequency of the so-called thermolabile MTHFR polymorphism, a proposed risk factor for CAD, was higher in Koreans than in other populations studied. The relatively low incidence of CAD in Korea suggests that this MTHFR polymorphism is not causative in this population.


Mol Cell Probes


Hong SH,Song J,Kim JQ




Has Abstract


2001-04-01 00:00:00














  • Successful quantification of cytomegalovirus DNA by competitive PCR and detection with capillary electrophoresis.

    abstract::Human cytomegalovirus (HCMV) is responsible for severe infections in immunocompromised patients. Viral load has recently been identified as one of the major risk factors for subsequent development of HCMV disease. In this context, we developed a protocol allowing rapid, sensitive and precise quantification of HCMV DNA...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Poirier-Toulemonde AS,Imbert-Marcille BM,Ferré-Aubineau V,Besse B,Le Roux MG,Cantarovich D,Billaudel S

    更新日期:1997-02-01 00:00:00

  • Development of a novel internal positive control for Taqman based assays.

    abstract::Development of rapid amplification assays for the detection and identification of biological threat agents has become a focus of diagnostic efforts in recent years. The use of real-time PCR assays as diagnostic tools depends upon two critical processes. First, nucleic acid purification must provide template that is bo...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hartman LJ,Coyne SR,Norwood DA

    更新日期:2005-02-01 00:00:00

  • Direct measurement of calpastatin subtypes by sandwich enzyme immunoassay using monoclonal antibodies.

    abstract::Six stable hybridoma cell lines secreting monoclonal antibodies to human calpastatin were established. All monoclonal antibodies belong to the IgG1 subclass and recognized different epitopes on calpastatin. At least two groups were distinguished; the first group was specific for muscle-type (M-) calpastatin and the se...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yokota H,Katayama M,Hino F,Kato I,Takano E,Maki M,Hatanaka M,Murachi T

    更新日期:1991-08-01 00:00:00

  • A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens.

    abstract::A multiplex PCR was developed that is capable of detecting four of the most important bacterial agents of atypical pneumonia, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in uncultured patient specimens. These organisms cause similar symptomologies and are often not...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: McDonough EA,Barrozo CP,Russell KL,Metzgar D

    更新日期:2005-10-01 00:00:00

  • Molecular detection and identification of enteroviruses in children admitted to a university hospital in Greece.

    abstract::Although enteroviral infections occur frequently during childhood, the circulation of particular serotypes has never been studied in Greece. The objectives of the present report were molecular detection and identification of human enteroviruses in children admitted with nonspecific febrile illness or meningitis to a u...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Siafakas N,Attilakos A,Vourli S,Stefos E,Meletiadis J,Nikolaidou P,Zerva L

    更新日期:2011-10-01 00:00:00

  • Genomic structure of the EPHA1 receptor tyrosine kinase gene.

    abstract::Some receptor tyrosine kinase genes are mutated in inherited and somatically acquired human cancers. To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions. The gene contains 18 exons, two...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Owshalimpur D,Kelley MJ

    更新日期:1999-06-01 00:00:00

  • Evaluation of two aneuploidy screening tests for chorionic villus samples: Multiplex ligation-dependent probe amplification and fluorescence in situ hybridization.

    abstract::The vast majority of first-trimester pregnancy losses are the consequence of numerical aberrations in fetal chromosomes, which may involve nearly all chromosomes. Although commercial probes for all chromosomes are available for multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridizat...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wu T,Zhu Y,Hong L,Lin Q,Chen C,Yang J,Ye L,Huang W,Zeng Y

    更新日期:2019-08-01 00:00:00

  • Construction of an internal control for the detection of Chlamydia pneumoniae by PCR.

    abstract::For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the same primer pair can be used to amplify the internal control and targ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ursi D,Ieven M,Van Bever HP,Goossens H

    更新日期:1998-08-01 00:00:00

  • Analysis of major histocompatibility complex class I gene transcription using oligonucleotide probes.

    abstract::Many highly homologous genes are present in the murine major histocompatibility complex (MHC) class I gene family. Consequently, it is difficult to distinguish between RNA transcripts of individual class I genes solely on the basis of nucleic acid hybridization analysis using DNA probes over 50 base pairs long. To avo...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Mellor AL

    更新日期:1987-09-01 00:00:00

  • Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66.

    abstract::Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three reactions, through SYBR green I melting curve analysis. The method utilizes type-specific primer se...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Tsakogiannis D,Papacharalampous M,Toska E,Kyriakopoulou Z,Dimitriou TG,Ruether IG,Komiotis D,Markoulatos P

    更新日期:2015-02-01 00:00:00

  • Direct identification of Pseudomonas aeruginosa from blood culture bottles by PCR-enzyme linked immunosorbent assay using oprI gene specific primers.

    abstract::A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for direct identification of Pseudomonas aeruginosa from positive BACTEC blood culture bottles. PCR primers were designed to target a 249 bp sequence of the oprI gene in P. aeruginosa. Biotin-labeled probe (PA3) targeted to the species-specific motif we...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Kurupati P,Kumarasinghe G,Laa Poh C

    更新日期:2005-12-01 00:00:00

  • Molecular cloning and nucleic acid sequencing of Chlamydia trachomatis 16S rRNA genes from patient samples lacking the cryptic plasmid.

    abstract::We have examined the relationship between Chlamydia trachomatis found in clinical samples in which the cryptic plasmid was absent and known serovars of C. trachomatis. PCR and RNase protection assays were used to compare 12 C. trachomatis serovars and a plasmidless L2 serovar strain with the reactivity of clinical spe...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: An Q,Olive DM

    更新日期:1994-10-01 00:00:00

  • Amplification of three hypervariable DNA regions by polymerase chain reaction for paternity determinations: comparison with conventional methods and DNA fingerprinting.

    abstract::The present study evaluates the usefulness of a PCR-based method for routine paternity testing in 35 paternity cases. This identification method which is based on amplification of three hypervariable genetic loci, apoB, D1S80 and HLA-DQ alpha, is compared, with regard to reliability and technical feasibility, to the c...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Helminen P,Sajantila A,Johnsson V,Lukka M,Ehnholm C,Peltonen L

    更新日期:1992-02-01 00:00:00

  • Identification of six novel CFTR mutations in a sample of Italian cystic fibrosis patients.

    abstract::The spectrum of cystic fibrosis (CF) mutations has been determined in many populations of different ethnic and geographic origins. However, in the south of Europe, the commonest mutation, delta F508, accounts for only about 50% of CF chromosomes, while identification of most of the other mutant alleles has not been ac...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Férec C,Novelli G,Verlingue C,Quéré I,Dallapiccola B,Audrézet MP,Mercier B

    更新日期:1995-04-01 00:00:00

  • A DNA probe assay using strand displacement amplification (SDA) and filtration to separate reacted and unreacted detector probes.

    abstract::Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a target DNA sequence. We performed SDA in the presence of a 5'-32P-oligodeoxynucleotide detector probe that contains a target binding sequence at its 3'-end and a recognition site for the restriction enzyme HincII at its 5'-end wh...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Walker GT,Nadeau JG,Linn CP

    更新日期:1995-12-01 00:00:00

  • Recombinant VirB5 protein as a potential serological marker for the diagnosis of bovine brucellosis.

    abstract::The molecular tag vaccine against Brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. They can discriminate vaccinated animals and humans from those naturally infected. In this study, we constructed a gene deletion mutant strain, B. abortus S19 virB5 with a molecu...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Tan W,Wang XR,Nie Y,Wang C,Cheng LQ,Wang XC,Zhang R,Yan GM

    更新日期:2012-06-01 00:00:00

  • Development and validation of a TTR-specific copy number screening tool, and application to potentially relevant patient cohorts.

    abstract::TTR amyloidosis (ATTR) is a fatal condition caused by extracellular deposits of misfolded transthyretin. Patients often present with cardiac disease, but manifestations may also involve other organs including the peripheral nervous system. ATTR is considered familial when heterozygous mutations in the TTR gene are pre...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Jahic A,Bock A,Duca F,Bonderman D,Mascherbauer J,Windhager R,Auer-Grumbach M,Beetz C

    更新日期:2018-10-01 00:00:00

  • Immunoblot and enzyme-linked immunosorbent assays of Campylobacter major outer-membrane protein and application to the differentiation of Campylobacter species.

    abstract::The major outer-membrane protein (MOMP) from Campylobacter jejuni NCTC 11168 was purified by solubilization in Triton X-100. Whole-cell proteins of Campylobacter species and the MOMP were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linke...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Taylor DE,Chang N

    更新日期:1987-09-01 00:00:00

  • Polymorphism rs3737787 of Upstream Stimulatory Factor 1 gene is associated with serum lipid phenotype in Nigerian population.

    abstract::Serum lipid profile which is determined by genotype-phenotype relationship plays a significant role in the development of cardiovascular disease. Upstream stimulatory factor 1 (USF1), has been reported to be associated with serum lipid levels in different population, hence, this study investigated the association of v...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Onadeko OT,Okunowo WO,Imaga NOA,Abdulrazaq MM,Onuminya OJ,Van-Lare TO,Nwosu M

    更新日期:2020-12-08 00:00:00

  • A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction.

    abstract::Quantification of nucleic acids, especially of mRNA, is increasingly important in biomedical research. The recently developed quantitative real-time polymerase chain reaction (PCR) - a highly sensitive technology for the rapid, accurate and reproducible quantification of gene expression - offers major advantages over ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Fronhoffs S,Totzke G,Stier S,Wernert N,Rothe M,Brüning T,Koch B,Sachinidis A,Vetter H,Ko Y

    更新日期:2002-04-01 00:00:00

  • Selection of stable reference genes for gene expression analysis in sweet potato (Ipomoea batatas L.).

    abstract::Gene expression analysis is one of the most common and important studies in biology and biomedicine. No matter for traditional blotting analysis or currently commonly used PCR strategy, all need a stable reference gene for normalizing the gene expression. To screen and select housekeeping genes as the most stable refe...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yu J,Su Y,Sun J,Liu J,Li Z,Zhang B

    更新日期:2020-10-01 00:00:00

  • Down-regulation of UBA6 exacerbates brain injury by inhibiting the activation of Notch signaling pathway to promote cerebral cell apoptosis in rat acute cerebral infarction model.

    abstract::This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

    更新日期:2020-10-01 00:00:00

  • Exploring transcriptional conservation between Ancylostoma caninum and Haemonchus contortus by oligonucleotide microarray and bioinformatic analyses.

    abstract::In this study, we identified, using an established oligonucleotide microarray platform for the parasitic nematode Haemonchus contortus, transcripts that are 'conserved' between serum-activated and non-activated L3s of Ancylostoma caninum (aL3 and L3, respectively) and H. contortus by cross-species hybridization (CSH) ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Cantacessi C,Loukas A,Campbell BE,Mulvenna J,Ong EK,Zhong W,Sternberg PW,Otranto D,Gasser RB

    更新日期:2009-02-01 00:00:00

  • The use of nested RT-PCR of prostate-specific membrane antigen in blood cells: implications for the detection of haematogenous neoplastic cells in patients with prostate adenocarcinoma.

    abstract::The aim of this study was to determine the presence of haematogenous neoplastic cells in patients with prostate cancer. Circulating prostate cells can be detected in cancer patients by using a nested-reverse transcriptase-polymerase chain reaction assay (RT-PCR), for prostate-specific membrane (PSM) antigen mRNA. This...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lucotte G,Mercier G,Burckel A

    更新日期:1998-12-01 00:00:00

  • Rapid PCR-based delineation of the porcine nodular worms, Oesophagostomum dentatum and O. quadrispinulatum.

    abstract::At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Newton LA,Chilton NB,Monti JR,Bjørn H,Várady M,Christensen CM,Gasser RB

    更新日期:1997-04-01 00:00:00

  • Polymorphisms in the Human SNAIL (SNAI1) gene.

    abstract::The human SNAIL is an important developmental protein involved in the formation of mesoderm and neural crest. The protein contains three classic and one atypical zinc-finger motif. The SNAI1 gene is composed of three exons. We have identified three SNPs in non-coding regions, two in the 5'UTR and one in intron 1, whic...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Okajima K,Paznekas WA,Burstyn T,Jabs EW

    更新日期:2001-02-01 00:00:00

  • In situ hybridization.

    abstract::In situ hybridization is the hybridization-mediated detection of specific nucleic acid sequences within structurally intact cells or tissues. As such it uniquely provides localization of nucleic acid superimposed on observable cellular and subcellular structural detail, allowing analysis unobtainable by other hybridiz...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Moench TR

    更新日期:1987-09-01 00:00:00

  • Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay.

    abstract::Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten b...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Liu CY,Song HQ,Zhang RL,Chen MX,Xu MJ,Ai L,Chen XG,Zhan XM,Liang SH,Yuan ZG,Lin RQ,Zhu XQ

    更新日期:2011-08-01 00:00:00

  • Development of PCR based assays for detection of lethal Holstein haplotype 1, 3 and 4 in Holstein Friesian cattle.

    abstract::Holstein haplotype (HH) 1, 3 and 4 are lethal mutations, responsible for early embryonic losses in Holstein Friesian (HF) cattle, worldwide. Three PCR based assays - tetra Amplification Refractory Mutation System PCR, PCR primer induced restriction analysis and PCR-restriction fragment length polymorphism techniques f...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Kumar A,Gupta ID,Mohan G,Vineeth MR,Ravi Kumar D,Jayakumar S,Niranjan SK

    更新日期:2020-04-01 00:00:00

  • Sensitive and quantitative detection of PCR-amplified HIV-1 DNA products by an enzyme linked immunoassay following solution hybridization with two differently labelled oligonucleotide probes.

    abstract::We have developed and evaluated an ELISA-based detection method for PCR-amplified HIV-1 DNA. The assay uses two oligonucleotide probes which are end-labelled at the 5'-end with biotin or digoxigenin, respectively. Upon solution hybridization of these probes which react with the same strand of amplified DNA product, th...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Böni J,Schüpbach J

    更新日期:1993-10-01 00:00:00