Cathepsin L-like genes of Trypanosoma vivax from Africa and South America--characterization, relationships and diagnostic implications.


:We characterized sequences from genes encoding cathepsin L-like (CatL-like) cysteine proteases from African and South American isolates of Trypanosoma vivax and T. vivax-like organisms, and evaluated their suitability as genetic markers for population structure analysis and diagnosis. Phylogenetic analysis of sequences corresponding to CatL-like catalytic domains revealed substantial polymorphism, and clades of sequences (TviCatL1-9) were separated by large genetic distances. TviCatL1-4 sequences were from cattle isolates from West Africa (Nigeria and Burkina Faso) and South America (Brazil and Venezuela), which belonged to the same T. vivax genotype. T. vivax-like genotypes from East Africa showed divergent sequences, including TviCatL5-7 for isolates from Mozambique and TviCatL8-9 for an isolate from Kenya. Phylogenetic analysis of CatL-like gene data supported the relationships among trypanosome species reflected in the phylogenies based on the analysis of small subunit (SSU) of ribosomal RNA gene sequence data. The discovery of different CatL-like sequences for each genotype, defined previously by ribosomal DNA data, indicate that these sequences provide useful targets for epidemiological and population genetic studies. Regions in CatL-like sequences shared by all T. vivax genotypes but not by other trypanosomes allowed the establishment of a specific and sensitive diagnostic PCR for epidemiological studies in South America and Africa.


Mol Cell Probes


Cortez AP,Rodrigues AC,Garcia HA,Neves L,Batista JS,Bengaly Z,Paiva F,Teixeira MM




Has Abstract


2009-02-01 00:00:00














  • Circ_016719 plays a critical role in neuron cell apoptosis induced by I/R via targeting miR-29c/Map2k6.

    abstract:BACKGROUND:Stroke is a leading cause of mortality worldwide. Rac-MAPK kinase 6 (Map2k6) plays important roles in cell proliferation and apoptosis. However, the role played by Map2k6 in stroke injury and the underlying mechanism of action remain unknown. METHODS:Mice received cerebral ischemia/reperfusion (I/R) injurie...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Tang C,Ou J,Kou L,Deng J,Luo S

    更新日期:2020-02-01 00:00:00

  • DNA typing of HLA-B27 by polymerase chain reaction.

    abstract::To find a specific method for HLA-B27 typing, we tested an HLA-B27-specific polymerase chain reaction. This method was used for screening 100 randomly selected blood donors, 10 of them being HLA-B27 positive. A flow cytometric method and this PCR method were compared. ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lucotte G,Burckel A

    更新日期:1997-08-01 00:00:00

  • Rapid PCR using nested primers of the 16S rRNA and the hippuricase (hip O) genes to detect Campylobacter jejuni and Campylobacter coli in environmental samples.

    abstract::Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bang DD,Wedderkopp A,Pedersen K,Madsen M

    更新日期:2002-10-01 00:00:00

  • Development of a novel internal positive control for Taqman based assays.

    abstract::Development of rapid amplification assays for the detection and identification of biological threat agents has become a focus of diagnostic efforts in recent years. The use of real-time PCR assays as diagnostic tools depends upon two critical processes. First, nucleic acid purification must provide template that is bo...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hartman LJ,Coyne SR,Norwood DA

    更新日期:2005-02-01 00:00:00

  • PTPN14 acts as a candidate tumor suppressor in prostate cancer and inhibits cell proliferation and invasion through modulating LATS1/YAP signaling.

    abstract::Protein tyrosine phosphatase, non-receptor type 14 (PTPN14) exerts a profound effect in the progression of multiple malignant tumors. However, whether PTPN14 plays a role in prostate cancer has not been well investigated. Herein, we evaluated the function and potential underlying mechanism of PTPN14 in prostate cancer...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wang R,Du Y,Shang J,Dang X,Niu G

    更新日期:2020-10-01 00:00:00

  • A simple procedure to differentiate ailA and ailNA gene variants among human pathogenic Yersinia enterocolitica strains.

    abstract::The identification and differentiation of the two variants of the ail gene, ailA from the more virulent American serotypes (08, 013a, 13b, 018, 020, 021) and ailNA from the less virulent non-American serotypes (03, 04, 05, 06, 09, 027 and 07, 8) was studied in a panel of 32 Yersinia enterocolitica human pathogenic iso...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sayada C,Picard B,Elion J,Krishnamoorthy R

    更新日期:1994-06-01 00:00:00

  • A new method for species identification and differentiation of Mycobacterium chelonae complex based on amplified hsp65 restriction analysis (AHSPRA).

    abstract::Members of the Mycobacterium chelonae complex (MCC), namely M. chelonae, Mycobacterium abscessus and Mycobacterium immunogenum, have been implicated in nosocomial infections and occupational respiratory illnesses like hypersensitivity pneumonitis (HP) associated with contaminated metalworking fluid (MWF) exposures. Cl...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Selvaraju SB,Khan IU,Yadav JS

    更新日期:2005-04-01 00:00:00

  • Detection of human cytomegalovirus in cervicovaginal cells by culture, in situ DNA hybridization and DNA amplification methods.

    abstract::The presence of human cytomegalovirus (HCMV) was tested in 388 cervicovaginal cells specimens obtained from the same number of pregnant women. HCMV was detected in 5.41%, 11.6% and 13.9% of these specimens by conventional culture, in situ DNA hybridization and polymerase chain reaction (PCR) methods, respectively. The...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yuan CF,Kao SM,Wang DC,Ng HT,Pao CC

    更新日期:1990-12-01 00:00:00

  • Twelve hour real-time PCR technique for the sensitive and specific detection of Salmonella in raw and ready-to-eat meat products.

    abstract::Rapid pathogen testing is vital to the food industry. Enzyme immunoassays (EIA) provide reliable negative results in 48 h, but a presumptive positive (suspect) EIA result must be confirmed by traditional culture methods, requiring an additional 72 h. Polymerase chain reaction (PCR) testing technology is accepted as an...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ellingson JL,Anderson JL,Carlson SA,Sharma VK

    更新日期:2004-02-01 00:00:00

  • Recombinant VirB5 protein as a potential serological marker for the diagnosis of bovine brucellosis.

    abstract::The molecular tag vaccine against Brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. They can discriminate vaccinated animals and humans from those naturally infected. In this study, we constructed a gene deletion mutant strain, B. abortus S19 virB5 with a molecu...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Tan W,Wang XR,Nie Y,Wang C,Cheng LQ,Wang XC,Zhang R,Yan GM

    更新日期:2012-06-01 00:00:00

  • Development of a TB green II-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine circovirus 2 and 3.

    abstract::Porcine circovirus 3 (PCV3), as a newly emerged circovirus, is widely distributed in pig populations worldwide. Co-infection of PCV2 and PCV3 has been reported frequently in clinical samples. In the present study, a TB Green II-based duplex real-time polymerase chain reaction (qPCR) was developed to rapidly and differ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Zhao Y,Han HY,Fan L,Tian RB,Cui JT,Li JY,Chen HY,Yang MF,Zheng LL

    更新日期:2019-06-01 00:00:00

  • Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay.

    abstract::Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten b...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Liu CY,Song HQ,Zhang RL,Chen MX,Xu MJ,Ai L,Chen XG,Zhan XM,Liang SH,Yuan ZG,Lin RQ,Zhu XQ

    更新日期:2011-08-01 00:00:00

  • A DNA microarray for identification of virulence and antimicrobial resistance genes in Salmonella serovars and Escherichia coli.

    abstract::Characterization of antimicrobial resistance and virulence gene profiles provides important information on the potential pathogenicity of bacteria. This information can be used to facilitate prompt and effective treatment of bacterial infections. We developed and tested a PCR-based microarray platform for detecting vi...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Chen S,Zhao S,McDermott PF,Schroeder CM,White DG,Meng J

    更新日期:2005-06-01 00:00:00

  • The use of nested RT-PCR of prostate-specific membrane antigen in blood cells: implications for the detection of haematogenous neoplastic cells in patients with prostate adenocarcinoma.

    abstract::The aim of this study was to determine the presence of haematogenous neoplastic cells in patients with prostate cancer. Circulating prostate cells can be detected in cancer patients by using a nested-reverse transcriptase-polymerase chain reaction assay (RT-PCR), for prostate-specific membrane (PSM) antigen mRNA. This...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lucotte G,Mercier G,Burckel A

    更新日期:1998-12-01 00:00:00

  • Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay.

    abstract::The aim of this study was the development of a real-time PCR for HIV DNA quantification in whole blood leucocytes providing an alternative assay to those already described, almost based on the gag gene detection. The technique (pbs-rtPCR assay) is more rapid (the whole assay required less than 5h), easy to perform, om...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Casabianca A,Gori C,Orlandi C,Forbici F,Federico Perno C,Magnani M

    更新日期:2007-10-01 00:00:00

  • Application of nucleic acid aptamers for detection of Apple stem pitting virus isolates.

    abstract::DNA aptamers (PSA-H and MT32) were applied for the detection of Apple stem pitting virus (ASPV) isolates using an Enzyme-Linked Oligonucleotide Assay (ELONA) and Western blot analysis. The specificity and effectiveness of aptamers were verified in comparison to a conventional Enzyme Linked Immunosorbent Assay (ELISA)....

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Komorowska B,Hasiów-Jaroszewska B,Minicka J

    更新日期:2017-12-01 00:00:00

  • LNA probe-based assay for the detection of Tomato black ring virus isolates.

    abstract::Tomato black ring virus (TBRV) infects a wide range of economically important plant species worldwide. In the present study we developed a locked nucleic acid (LNA) real-time RT-PCR assay for accurate detection of genetically diverse TBRV isolates collected from different hosts. The assay based on the LNA probe has a ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hasiów-Jaroszewska B,Rymelska N,Borodynko N

    更新日期:2015-02-01 00:00:00

  • GT repeat polymorphism in the 5' flanking region of the human growth hormone receptor gene.

    abstract::A polymorphic GT dinucleotide repeat sequence has been identified in the 5' flanking region of the human growth hormone receptor (hGHR) gene on chromosome 5p13.1-p12, within the promoter region of the V9 5'UTR exon. Thirteen alleles have been identified in 50 non-related individuals, with an observed heterozygosity of...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Hadjiyannakis S,Zheng H,Hendy GN,Goodyer CG

    更新日期:2001-08-01 00:00:00

  • Design considerations and effects of LNA in PCR primers.

    abstract::The effects of comprehensive LNA substitution in PCR primers for amplification of human genomic DNA targets are presented in this report. Previous research with LNA in other applications has shown interesting properties for molecular hybridization including enhanced specificity in allele-specific PCR. Here we systemat...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Latorra D,Arar K,Hurley JM

    更新日期:2003-10-01 00:00:00

  • Development and evaluation of the polymerase chain reaction method for diagnosis of Mycoplasma gallisepticum infection in chickens.

    abstract::A polymerase chain reaction (PCR) method specific for Mycoplasma gallisepticum (MG) was evaluated. The PCR method was found to detect as few as two colour changing units (CCU) of MG and did not give false positive reactions with other avian mycoplasmas. In chickens inoculated with either MG or Mycoplasma synoviae (MS)...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Slavik MF,Wang RF,Cao WW

    更新日期:1993-12-01 00:00:00

  • Quantitative PCR evaluation of deletions/duplications identified by array CGH.

    abstract::Genomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentia...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Baldan F,Passon N,Burra S,Demori E,Russo PD,Damante G

    更新日期:2019-08-01 00:00:00

  • Mutation screening of the chromosome 8q24.3-human activity-regulated cytoskeleton-associated gene (ARC) in idiopathic generalized epilepsy.

    abstract::Idiopathic generalized epilepsy (IGE) comprises a heterogeneous group of disorders, in which a high genetic predisposition and a complex mode of inheritance have been suggested. However, genes, which confer liability to common IGE subtypes including juvenile myoclonic epilepsy (JME) and childhood absence epilepsy (CAE...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Haug K,Kremerskothen J,Hallmann K,Sander T,Dullinger J,Rau B,Beyenburg S,Lentze MJ,Barnekow A,Elger CE,Propping P,Heils A

    更新日期:2000-08-01 00:00:00

  • Development of a p28-based PCR assay for Ehrlichia chaffeensis.

    abstract::Detection of Ehrlichia chaffeensis is necessary to study interactions between the parasite and its vertebrate and invertebrate hosts. The purpose of this study was to develop a sensitive, specific PCR assay for E. chaffeensis based on the outer membrane protein gene, p28. Candidate primer sets were identified and rank...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wagner ER,Bremer WG,Rikihisa Y,Ewing SA,Needham GR,Unver A,Wang X,Stich RW

    更新日期:2004-04-01 00:00:00

  • Use of a nested PCR method for the detection of astrovirus serotype 1 in human faecal material.

    abstract::In this paper we describe a reverse-transcription nested polymerase chain reaction method for detecting human astrovirus serotype 1. It has been evaluated on 56 UK diarrhoeal stool specimens and six non-UK specimens. The method has greater sensitivity than electron microscopy and may be a useful test in areas such as ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Shi M,Sikotra S,Lee T,Kurtz JB,Getty B,Hart CA,Myint SH

    更新日期:1994-12-01 00:00:00

  • Use of RAPD for the detection of genetic variation in the human blood fluke, Schistosoma japonicum, from mainland China.

    abstract::A random amplified polymorphic DNA (RAPD) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of Schistosoma japonicum from seven geographical locations (Sj1: Zhejiang; Sj2: Anhui; Sj3: Jiangxi; Sj4: Hunan; Sj5: Hubei; Sj6: Sichuan; Sj7: Yunnan) of the People's Republic of China. D...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Gasser RB,Bao-Zhen Q,Nansen P,Johansen MV,Bøgh H

    更新日期:1996-10-01 00:00:00

  • Rapid control of purity for the cytoplasm of male-sterile seed stocks by means of a dot hybridization assay.

    abstract::To produce hybrids, one member of the parental line is genetically made male-sterile. This male-sterile trait is encoded by mitochondria so that it is maternally inherited. Consequently, the progeny of a male-sterile plant is fully sterile. Nevertheless, during the handling of cytoplasmic male-sterile seed stocks, som...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Santoni S,Faivre-Rampant P,Moreau E,Bervillé A

    更新日期:1991-02-01 00:00:00

  • Expression patterns of miR-146a and miR-146b in mastitis infected dairy cattle.

    abstract::This study reports a significant up-regulation of bta-miR-146a and bta-miR-146b expression levels in bovine mammary tissues infected with subclinical, clinical and experimental mastitis. Potential target genes are involved in multiple immunological pathways. These results suggest a regulatory function of both miRNAs f...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wang XP,Luoreng ZM,Zan LS,Raza SH,Li F,Li N,Liu S

    更新日期:2016-10-01 00:00:00

  • Detection of Pseudomonas aeruginosa in sputum from cystic fibrosis patients by the polymerase chain reaction.

    abstract::A DNA amplification procedure using heat stable Taq polymerase and the polymerase chain reaction is described for the detection of Pseudomonas aeruginosa in specimens from cystic fibrosis patients. A set of primers was selected on the basis of the nucleotide sequence of the algD gene encoding GDP mannose dehydrogenase...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: McIntosh I,Govan JR,Brock DJ

    更新日期:1992-08-01 00:00:00

  • On-line moisture determination of ore concentrates 'a review of traditional methods and introduction of a novel solution'.

    abstract::The manual gravimetric drying moisture determination methods currently employed by most mineral processing plants fail to provide timely and accurate information required for automatic control. The costs associated with transporting and handling concentrates still represent a major portion of the overall treatment pri...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Cancilla PA,Barrette P,Rosenblum F

    更新日期:2002-12-01 00:00:00

  • CircHIPK3 regulates melanoma cell behaviors by binding with miR-215-5p to upregulate YY1.

    abstract:OBJECT:To investigate the role of circHIPK3 in melanoma. METHODS:Bioinformatics analysis and experiments including RT-qPCR, Pearson's correlation analysis, luciferase reporter, Western blot, and RIP assays were applied to explore the function and mechanism of circHIPK3 in melanoma. RESULTS:CircHIPK3 expression was st...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Zhu X,Sun J

    更新日期:2020-10-01 00:00:00