Molecular diagnosis of genital HPV DNA types by polymerase chain reaction and sensitivity-standardized filter in situ hybridization in randomly sampled cohorts of Singapore women.


:Infection of the cervix uteri with various types of human papillomaviruses is generally considered a necessary factor in the etiology of cancer of the cervix uteri. In many human populations throughout the world, approximately 90% of cervical carcinomas are found to harbour HPV genomes, as judged by Southern blot hybridization, while only a few percent of the cervical smears of asymptomatic individuals contain viral DNA, as assessed by filter in situ hybridization. To obtain corresponding epidemiological data from Singapore, we analysed two groups of 740 and 130 individuals by filter in situ hybridization, and found 4.1% and 6.9% of them to be HPV positive, with HPV 16 and HPV 31 being the predominant types. In consideration of the limitations of filter in situ hybridization, namely low sensitivity and a tendency to suggest false positives due to contaminants, including blood, we analysed the cervical smears of two further groups of 52 and 50 individuals by the polymerase chain reaction for infection by HPV 16 and HPV 18 respectively. With this test, 61% and 14% of the cervical smears proved to be HPV 16 and HPV 18 DNA positive respectively. We conclude that in Singapore, if not worldwide, the majority of the population the population is infected by genital HPV types, suggesting that factors other than HPV infection are ultimately rate-limiting in cervical carcinogenesis.


Mol Cell Probes


Chow V,Tham KM,Yeo-Gloss M,Lim-Tan SK,Sng I,Thirumoorthy T,Bernard HU




Has Abstract


1990-04-01 00:00:00














  • Specific PCR primers for Cryptosporidium parvum with extra high sensitivity.

    abstract::One pair of high-sensitive polymerase chain reaction (PCR) primers for Cryptosporidium parvum was constructed based on the sequence of random amplified polymorphic DNA. PCR with this primer pair amplified only the DNA of C. parvum, not the control DNA including Cryptosporidium muris. This primer pair had most advantag...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wu Z,Nagano I,Matsuo A,Uga S,Kimata I,Iseki M,Takahashi Y

    更新日期:2000-02-01 00:00:00

  • A novel technique for rapid automated genotyping of DNA polymorphisms in the mouse.

    abstract::The ability to rapidly and reliably genotype mice is an important concern. Traditional methods employ labour intensive and time consuming techniques such as test crossing, gel electrophoresis or nucleic acid hybridization. Here we show that a new molecular biology workstation, the WAVE DNA Fragment Analysis System, ca...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Kuklin A,Davis AP,Hecker KH,Gjerde DT,Taylor PD

    更新日期:1999-06-01 00:00:00

  • A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction.

    abstract::Quantification of nucleic acids, especially of mRNA, is increasingly important in biomedical research. The recently developed quantitative real-time polymerase chain reaction (PCR) - a highly sensitive technology for the rapid, accurate and reproducible quantification of gene expression - offers major advantages over ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Fronhoffs S,Totzke G,Stier S,Wernert N,Rothe M,Brüning T,Koch B,Sachinidis A,Vetter H,Ko Y

    更新日期:2002-04-01 00:00:00

  • Genetic differentiation and phylogeny of 27 sheep populations based on structural gene loci.

    abstract::To explore the genetic divergence and phylogeny of Chinese indigenous sheep breeds, in the current study, we analyzed the polymorphisms of 5 structural loci in ten sheep populations, including Sishui Fur, Sunite, Wurank, Bayinbuluke, Altay, Small-Tailed Han, Wadi, Tan, Tong and Hu sheep. The data were then compared wi...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Li M,Xia H,Chen D,Ji D,Kenji T,Li R,Liao X,Mao Y,Sun W,Geng R,Yang Z

    更新日期:2018-02-01 00:00:00

  • Use of RAPD for the detection of genetic variation in the human blood fluke, Schistosoma japonicum, from mainland China.

    abstract::A random amplified polymorphic DNA (RAPD) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of Schistosoma japonicum from seven geographical locations (Sj1: Zhejiang; Sj2: Anhui; Sj3: Jiangxi; Sj4: Hunan; Sj5: Hubei; Sj6: Sichuan; Sj7: Yunnan) of the People's Republic of China. D...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Gasser RB,Bao-Zhen Q,Nansen P,Johansen MV,Bøgh H

    更新日期:1996-10-01 00:00:00

  • Detection of catechol-O-methyltransferase Val158Met polymorphism by a simple one-step tetra-primer amplification refractory mutation system-PCR.

    abstract::The G-->A transition at nucleotide 21881 of the human catechol-O-methyltransferase (COMT) gene represents a functional genetic polymorphism (Val158Met), rendering an enzyme with reduced activity that has been associated with psychiatric disorders and estrogen-related cancers. A new method for the detection of this pol...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Ruiz-Sanz JI,Aurrekoetxea I,Ruiz del Agua A,Ruiz-Larrea MB

    更新日期:2007-06-01 00:00:00

  • Specific detection of common pathogens of acute bacterial meningitis using an internally controlled tetraplex-PCR assay.

    abstract::Accurate and timely diagnosis of acute bacterial meningitis is critical for antimicrobial treatment of patients. Although PCR-based methods have been widely used for the diagnosis of acute meningitis caused by bacterial pathogens, the main disadvantage of these methods is their high cost. This disadvantage has hampere...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Farahani H,Ghaznavi-Rad E,Mondanizadeh M,MirabSamiee S,Khansarinejad B

    更新日期:2016-08-01 00:00:00

  • Detection of PCR products using PNA strand invasion.

    abstract::The unique ability of homopyrimidine peptide nucleic acid (PNA) to strand invade homopurine sites of duplex DNA offers a potential alternative to existing techniques for rapid detection of PCR products. From gel shift studies, PNA was found to specifically strand invade homopurine sites that had been incorporated into...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Drewe LJ,Brightwell G,Hall EA

    更新日期:2000-10-01 00:00:00

  • Development of a p28-based PCR assay for Ehrlichia chaffeensis.

    abstract::Detection of Ehrlichia chaffeensis is necessary to study interactions between the parasite and its vertebrate and invertebrate hosts. The purpose of this study was to develop a sensitive, specific PCR assay for E. chaffeensis based on the outer membrane protein gene, p28. Candidate primer sets were identified and rank...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Wagner ER,Bremer WG,Rikihisa Y,Ewing SA,Needham GR,Unver A,Wang X,Stich RW

    更新日期:2004-04-01 00:00:00

  • A novel highly informative polyA microsatellite on the telomeric side of the INK4a/ARF locus.

    abstract::The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Chaubert P,Burri N,Cousin P,Shaw P

    更新日期:2001-06-01 00:00:00

  • Immunoblot and enzyme-linked immunosorbent assays of Campylobacter major outer-membrane protein and application to the differentiation of Campylobacter species.

    abstract::The major outer-membrane protein (MOMP) from Campylobacter jejuni NCTC 11168 was purified by solubilization in Triton X-100. Whole-cell proteins of Campylobacter species and the MOMP were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linke...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Taylor DE,Chang N

    更新日期:1987-09-01 00:00:00

  • Integrated multiplex ligation dependent probe amplification (MLPA) assays for the detection of alterations in the HEXB, GM2A and SMARCAL1 genes to support the diagnosis of Morbus Sandhoff, M. Tay-Sachs variant AB and Schimke immuno-osseous dysplasia in hu

    abstract::Multiplex ligation dependent probe amplification (MLPA) assays were designed for the genes HEXB (OMIM: 606873), GM2A (OMIM: 613109) and SMARCAL1 (OMIM: 606622) of humans. Two sets of synthetic MLPA probes for these coding exons were tested. Changes in copy numbers were detected as well as single nucleotide polymorphis...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sobek AK,Evers C,Dekomien G

    更新日期:2013-02-01 00:00:00

  • Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes.

    abstract::An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Chantratita W,Henchal EA,Yoosook C

    更新日期:1989-12-01 00:00:00

  • Development and validation of a quantitative real time PCR assay for BK virus.

    abstract::Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. We developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine. Consider...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Mitui M,Leos NK,Lacey D,Doern C,Rogers BB,Park JY

    更新日期:2013-10-01 00:00:00

  • Direct measurement of calpastatin subtypes by sandwich enzyme immunoassay using monoclonal antibodies.

    abstract::Six stable hybridoma cell lines secreting monoclonal antibodies to human calpastatin were established. All monoclonal antibodies belong to the IgG1 subclass and recognized different epitopes on calpastatin. At least two groups were distinguished; the first group was specific for muscle-type (M-) calpastatin and the se...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yokota H,Katayama M,Hino F,Kato I,Takano E,Maki M,Hatanaka M,Murachi T

    更新日期:1991-08-01 00:00:00

  • Opportunities and challenges for the application of microfluidic technologies in point-of-care veterinary diagnostics.

    abstract::There is a growing need for low-cost, rapid and reliable diagnostic results in veterinary medicine. Point-of-care (POC) tests have tremendous advantages over existing laboratory-based tests, due to their intrinsic low-cost and rapidity. A considerable number of POC tests are presently available, mostly in dipstick or ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Busin V,Wells B,Kersaudy-Kerhoas M,Shu W,Burgess ST

    更新日期:2016-10-01 00:00:00

  • The use of nested RT-PCR of prostate-specific membrane antigen in blood cells: implications for the detection of haematogenous neoplastic cells in patients with prostate adenocarcinoma.

    abstract::The aim of this study was to determine the presence of haematogenous neoplastic cells in patients with prostate cancer. Circulating prostate cells can be detected in cancer patients by using a nested-reverse transcriptase-polymerase chain reaction assay (RT-PCR), for prostate-specific membrane (PSM) antigen mRNA. This...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Lucotte G,Mercier G,Burckel A

    更新日期:1998-12-01 00:00:00

  • Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

    abstract::Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Axton RA,Brock DJ

    更新日期:1994-06-01 00:00:00

  • Selection of stable reference genes for gene expression analysis in sweet potato (Ipomoea batatas L.).

    abstract::Gene expression analysis is one of the most common and important studies in biology and biomedicine. No matter for traditional blotting analysis or currently commonly used PCR strategy, all need a stable reference gene for normalizing the gene expression. To screen and select housekeeping genes as the most stable refe...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yu J,Su Y,Sun J,Liu J,Li Z,Zhang B

    更新日期:2020-10-01 00:00:00

  • The use of real-time polymerase chain reaction with high resolution melting (real-time PCR-HRM) analysis for the detection and discrimination of nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus.

    abstract::The real-time PCR-HRM analysis was developed for the detection and discrimination of the quarantine nematode Bursaphelenchus xylophilus and Bursaphelenchus mucronatus. A set of primers was designed to target the ITS region of rDNA. The results have demonstrated that this analysis is a valuable tool for differentiation...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Filipiak A,Hasiów-Jaroszewska B

    更新日期:2016-04-01 00:00:00

  • miR-410-5p promotes the development of diabetic cardiomyopathy by suppressing PIM1-induced anti-apoptosis.

    abstract::Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myoc...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Xia X,Liang Y,Zheng W,Lin D,Sun S

    更新日期:2020-08-01 00:00:00

  • Development of a multiplex PCR assay for the identification of pathogenic genes of Escherichia coli in milk and milk products.

    abstract::A multiplex PCR for the simultaneous detection of some pathogenic genes of enteropathogenic, enterotoxigenic and verocytotoxin-producing Escherichia coli was developed. In this study primers found in literature as well as primers to the purpose designed were used. In this way, it was possible to generate specific frag...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Bottero MT,Dalmasso A,Soglia D,Rosati S,Decastelli L,Civera T

    更新日期:2004-08-01 00:00:00

  • High-throughput qualitative multiplex 5' nuclease assay using post-only PCR analysis.

    abstract::Homogenous fluorescence PCR assays offer distinct advantages for qualitative testing and are gaining immense popularity in fields like diagnostic microbiology. To meet the demand of high-volume laboratories, we developed a protocol for qualitative multiplex 5' nuclease assays using post-only PCR analysis. This novel a...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Antonishyn NA,McDonald RR,Chan EL,Horsman GB

    更新日期:2005-10-01 00:00:00

  • Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus.

    abstract::Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is a highly contagious intestinal disease, resulting in substantial economic losses to the swine industry worldwide. In this study, three assays, namely a conventional reverse transcription-polymerase chain reaction (RT-PCR), a SYBR Green...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Zhou X,Zhang T,Song D,Huang T,Peng Q,Chen Y,Li A,Zhang F,Wu Q,Ye Y,Tang Y

    更新日期:2017-06-01 00:00:00

  • Cell preservation methods and its application to studying rare disease.

    abstract::The ability to preserve and transport human cells in a stable medium over long distances is critical to collaborative efforts and the advancement of knowledge in the study of human disease. This is particularly important in the study of rare diseases. Recently, advancements in the understanding of renal ciliopathies h...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章,评审


    authors: Dewhurst RM,Molinari E,Sayer JA

    更新日期:2021-01-08 00:00:00

  • Use of DNA restriction endonuclease digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from human and animal hosts.

    abstract::Variation amongst strains of Helicobacter pylori and Helicobacter mustelae was examined by DNA restriction endonuclease digestion and rRNA gene patterns generated using a non-radioactive probe. Chromosomal DNA was extracted from 30 cultures of H. pylori from human, Rhesus monkey and pig gastric mucosa, and from three ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Morgan DD,Owen RJ

    更新日期:1990-08-01 00:00:00

  • A simple procedure to differentiate ailA and ailNA gene variants among human pathogenic Yersinia enterocolitica strains.

    abstract::The identification and differentiation of the two variants of the ail gene, ailA from the more virulent American serotypes (08, 013a, 13b, 018, 020, 021) and ailNA from the less virulent non-American serotypes (03, 04, 05, 06, 09, 027 and 07, 8) was studied in a panel of 32 Yersinia enterocolitica human pathogenic iso...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Sayada C,Picard B,Elion J,Krishnamoorthy R

    更新日期:1994-06-01 00:00:00

  • MiR-34a suppresses the proliferation and invasion of gastric cancer by modulating PDL1 in the immune microenvironment.

    abstract:OBJECTIVES:As one of the most serious malignant carcinomas that threaten the life of sufferers constantly, gastric cancer has attracted a lot of interest among researchers. miR-34a, a member of hundreds of microRNAs (miRNAs), has been elucidated to exert a suppressive role in gastric cancer tumorgenesis based on previo...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Yong H,Fu J,Gao G,Shi H,Zheng D,Zhou X

    更新日期:2020-10-01 00:00:00

  • A novel peptide-based recognition probe for the sensitive detection of CD44 on breast cancer stem cells.

    abstract::Metastasis and recurrence of breast cancer remain significant clinical problems. The expression level of CD44 protein is higher in breast cancer-initiating cancer stem cells; therefore, the early detection of CD44 using a sensitive diagnostic probe is important for breast cancer diagnosis and therapeutic purposes. In ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Cho JH,Lee SC,Ha NR,Lee SJ,Yoon MY

    更新日期:2015-12-01 00:00:00

  • DNA probe and polymerase chain reaction procedure for the specific detection of Serpulina hyodysenteriae.

    abstract::Serpulina (Treponema) hyodysenteriae, a Gram-negative anaerobic spirochete, is the causative agent of swine dysentery, a mucohaemorrhagic diarrheal disease in which lesions are confined to the large intestine of pigs. A DNA probe and polymerase chain reaction (PCR) amplification procedures which are specific, rapid , ...

    journal_title:Molecular and cellular probes

    pub_type: 杂志文章


    authors: Harel J,Forget C

    更新日期:1995-04-01 00:00:00