LNA probe-based assay for the detection of Tomato black ring virus isolates.

abstract：:Variable number tandem repeat (VNTR) is a frequently employed typing method of Mycobacterium avium paratuberculosis (MAP) isolates. Based on whole genome sequencing in a previous study, allelic diversity at some VNTR loci seems to over- or under-estimate the actual phylogenetic variance among isolates. Interestingly, ...

journal_title：Molecular and cellular probes

authors： Fawzy A,Zschöck M,Ewers C,Eisenberg T

更新日期：2016-06-01 00:00:00

abstract：:Yersinia pestis, the causative agent of plague mainly infects rodents, while humans are the accidental host. The conventional diagnostic methods available for Y. pestis exhibit cross-reactivity with other enteropathogenic bacteria which makes its detection difficult. Rapid and reliable point-of-care detection of Y. pe...

journal_title：Molecular and cellular probes

authors： Singh R,Pal V,Tripathi NK,Goel AK

更新日期：2020-12-01 00:00:00

abstract：:The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...

journal_title：Molecular and cellular probes

authors： Russo S,Mencarelli M,Cavalleri F,Selicorni A,Cogliati F,Larizza L

更新日期：2003-12-01 00:00:00

abstract：:The major outer-membrane protein (MOMP) from Campylobacter jejuni NCTC 11168 was purified by solubilization in Triton X-100. Whole-cell proteins of Campylobacter species and the MOMP were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linke...

journal_title：Molecular and cellular probes

authors： Taylor DE,Chang N

更新日期：1987-09-01 00:00:00

abstract：:The detection limits of the polymerase chain reaction (PCR) for Mycoplasma pneumoniae were determined using specimens from persons known to have had M. pneumoniae pneumonia. Four primers were selected from the known sequence of the P1 gene. The primer pair (P1-178 and P1-809) which generates a 631 fragment gave the lo...

journal_title：Molecular and cellular probes

authors： Leng Z,Kenny GE,Roberts MC

更新日期：1994-04-01 00:00:00

abstract：:The aim of this study was the development of a real-time PCR for HIV DNA quantification in whole blood leucocytes providing an alternative assay to those already described, almost based on the gag gene detection. The technique (pbs-rtPCR assay) is more rapid (the whole assay required less than 5h), easy to perform, om...

journal_title：Molecular and cellular probes

authors： Casabianca A,Gori C,Orlandi C,Forbici F,Federico Perno C,Magnani M

更新日期：2007-10-01 00:00:00

abstract：:Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is a highly contagious intestinal disease, resulting in substantial economic losses to the swine industry worldwide. In this study, three assays, namely a conventional reverse transcription-polymerase chain reaction (RT-PCR), a SYBR Green...

journal_title：Molecular and cellular probes

authors： Zhou X,Zhang T,Song D,Huang T,Peng Q,Chen Y,Li A,Zhang F,Wu Q,Ye Y,Tang Y

更新日期：2017-06-01 00:00:00

abstract：:Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

journal_title：Molecular and cellular probes

authors： Bang DD,Wedderkopp A,Pedersen K,Madsen M

更新日期：2002-10-01 00:00:00

abstract：OBJECTIVES:As one of the most serious malignant carcinomas that threaten the life of sufferers constantly, gastric cancer has attracted a lot of interest among researchers. miR-34a, a member of hundreds of microRNAs (miRNAs), has been elucidated to exert a suppressive role in gastric cancer tumorgenesis based on previo...

journal_title：Molecular and cellular probes

authors： Yong H,Fu J,Gao G,Shi H,Zheng D,Zhou X

更新日期：2020-10-01 00:00:00

abstract：:The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs i...

journal_title：Molecular and cellular probes

authors： Nagaraju J,Kathirvel M,Subbaiah EV,Muthulakshmi M,Kumar LD

更新日期：2002-02-01 00:00:00

abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-rea...

journal_title：Molecular and cellular probes

authors： Chen HT,Zhang J,Ma YP,Ma LN,Ding YZ,Liu XT,Cai XP,Ma LQ,Zhang YG,Liu YS

更新日期：2010-04-01 00:00:00

abstract：:Shigella spp are exquisitely fastidious Gram negative organisms that frequently get missed in the detection by traditional culture methods. For this reason, this work has adapted a classical PCR for detection of Shigella in food and stool specimens to real-time PCR using the SYBR Green format. This method follows a me...

journal_title：Molecular and cellular probes

authors： Mokhtari W,Nsaibia S,Gharbi A,Aouni M

更新日期：2013-02-01 00:00:00

abstract：:Genetic polymorphism among patients with acute lymphoblastic leukemia (ALL) is an important factor in the effectiveness and toxicity of anti-leukemic drugs. Genotyping of various polymorphisms that impact the outcome of anti-leukemic drug therapy (pharmacogenetics) presents an attractive approach for developing indivi...

journal_title：Molecular and cellular probes

authors： Kang HJ,Oh Y,Chun SM,Seo YJ,Shin HY,Kim CW,Ahn HS,Han BD

更新日期：2008-06-01 00:00:00

abstract：:We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant d...

journal_title：Molecular and cellular probes

authors： Hong SH,Song J,Kim JQ

更新日期：2001-04-01 00:00:00

abstract：:Citrus plants are natural hosts of five viroid species and a large number of sequence variants. Because of their small size, viroids lend themselves to various RT-PCR approaches for their detection and further characterization. The one-step RT-PCR approach proposed here is based on the synthesis of viroid-cDNA by reve...

journal_title：Molecular and cellular probes

authors： Bernad L,Duran-Vila N

更新日期：2006-04-01 00:00:00

abstract：:Mutations in the WDR45 gene have been identified as causative for the only X-linked type of neurodegeneration with brain iron accumulation (NBIA), clinically characterized by global developmental delay in childhood, followed by a secondary neurological decline with parkinsonism and/or dementia in adolescence or early ...

journal_title：Molecular and cellular probes

authors： Hoffjan S,Ibisler A,Tschentscher A,Dekomien G,Bidinost C,Rosa AL

更新日期：2016-02-01 00:00:00

abstract：:Although enteroviral infections occur frequently during childhood, the circulation of particular serotypes has never been studied in Greece. The objectives of the present report were molecular detection and identification of human enteroviruses in children admitted with nonspecific febrile illness or meningitis to a u...

journal_title：Molecular and cellular probes

authors： Siafakas N,Attilakos A,Vourli S,Stefos E,Meletiadis J,Nikolaidou P,Zerva L

更新日期：2011-10-01 00:00:00

abstract：:Swine are implicated as the principal animal reservoir for plasmid-bearing Yersinia enterocolitica (YEP(+)) strains that are pathogenic to humans. To evaluate the utility of the PCR for detection of YEP(+) strains in naturally-contaminated pig feces, samples were first enriched in Irgasan ticarcillin potassium chlorat...

journal_title：Molecular and cellular probes

authors： Bhaduri S

更新日期：2002-06-01 00:00:00

abstract：:Diabetic cardiomyopathy (DCM) is a common complication of diabetes mellitus that can cause many severe symptoms, such as heart failure, arrhythmia, and sudden death. However, the molecular mechanisms underlying cardiac dysfunction in DCM remain elusive. In this study, we found that miR-410-5p was increased in the myoc...

journal_title：Molecular and cellular probes

authors： Xia X,Liang Y,Zheng W,Lin D,Sun S

更新日期：2020-08-01 00:00:00

abstract：:This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

journal_title：Molecular and cellular probes

authors： Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

更新日期：2020-10-01 00:00:00

abstract：:The ability to rapidly and reliably genotype mice is an important concern. Traditional methods employ labour intensive and time consuming techniques such as test crossing, gel electrophoresis or nucleic acid hybridization. Here we show that a new molecular biology workstation, the WAVE DNA Fragment Analysis System, ca...

journal_title：Molecular and cellular probes

authors： Kuklin A,Davis AP,Hecker KH,Gjerde DT,Taylor PD

更新日期：1999-06-01 00:00:00

abstract：:The real-time PCR-HRM analysis was developed for the detection and discrimination of the quarantine nematode Bursaphelenchus xylophilus and Bursaphelenchus mucronatus. A set of primers was designed to target the ITS region of rDNA. The results have demonstrated that this analysis is a valuable tool for differentiation...

journal_title：Molecular and cellular probes

authors： Filipiak A,Hasiów-Jaroszewska B

更新日期：2016-04-01 00:00:00

abstract：:PCR detection and quantification of vaginal lactobacilli remains problematic because of the high level of genetic heterogeneity and taxonomic complexity within the genus Lactobacillus. The aim of the present study was to identify conserved sequences among the genomes of major species of vaginal lactobacilli that could...

journal_title：Molecular and cellular probes

authors： Demkin VV,Koshechkin SI,Slesarev A

更新日期：2017-04-01 00:00:00

abstract：:A simple and rapid method, PCR-restriction enzyme analysis (PCR-RE) for BK virus (BKV) typing was developed, based on the presence of type-specific restriction enzyme sites in a 327 bp PCR-generated fragment which partially encodes the VP1 protein. The enzymes, Alu I, Xmn I and Ava II, were used to digest the PCR prod...

journal_title：Molecular and cellular probes

authors： Jin L

更新日期：1993-08-01 00:00:00

abstract：:The recently known coronavirus, SARS-CoV-2, has turn into the greatest global health challenge, affecting a large number of societies. The lack of specific treatment and gold-standard diagnostic system has made the situation more complicated. Efforts have led to production of several diagnostic kits that are associate...

journal_title：Molecular and cellular probes

authors： Torabi R,Ranjbar R,Halaji M,Heiat M

更新日期：2020-10-01 00:00:00

abstract：:Polymorphisms (rs1801282, rs8192678, rs7903146) of peroxisome proliferator-activated receptor gamma (PPARG), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PPARGC1A) and transcription factor 7-like 2 (TCF7L2) have recently been associated with different diseases, mainly type 2 diabetes. An assay...

journal_title：Molecular and cellular probes

authors： Habalová V,Klimcáková L,Zidzik J,Tkác I

更新日期：2009-02-01 00:00:00

abstract：:Analysis of single nucleotide polymorphisms by PCR with fluorescence resonance energy transfer (FRET) probes often can produce a result where the melting peak corresponding to perfectly matched sequence (A allele) has a smaller area than the peak corresponding to the allele with a mismatch (B allele). This imbalance c...

journal_title：Molecular and cellular probes

authors： Martínez-García A,Sastre I,Tenorio R,Bullido MJ

更新日期：2004-08-01 00:00:00

abstract：:A beacon probe was designed to detect one of the two documented single nucleotide changes in IS481 target allele of Bordetella holmesii genome as compared to Bordetella pertussis. PCR amplified product targeting a region of IS481 in presence of the probe was subjected to a post-PCR hybridization and melting cycle. Hyb...

journal_title：Molecular and cellular probes

authors： Poddar SK

更新日期：2003-04-01 00:00:00

abstract：:The detection of hepatitis C virus (HCV) RNA by nested polymerase chain reaction (PCR) is believed to be the most reliable method to diagnose HCV infections. A pitfall of nested PCR is that it is prone to contamination. Single step reverse transcription-PCR (RT-PCR) was performed, prospectively, on 80 sera from 59 pat...

journal_title：Molecular and cellular probes

authors： Aslanzadeh J,Padilla BB,Shanley JD

更新日期：1996-06-01 00:00:00

abstract：:A real-time assay for DNA methyltransferase (MTase) activity has been developed. A hemimethylated smart probe is used as the substrate for DNA MTase. Cleavage of the methylated product leads to separation of fluorophore from quencher, giving a proportional increase in fluorescence. The method permits real-time monitor...

journal_title：Molecular and cellular probes

authors： Jin S,Liu H,Xia K,Ma C,He H,Wang K

更新日期：2016-06-01 00:00:00