Specific PCR primers for Cryptosporidium parvum with extra high sensitivity.

abstract：:Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific prim...

journal_title：Molecular and cellular probes

authors： Wan C,Cheng L,Chen C,Liu R,Shi S,Fu G,Chen H,Fu Q,Huang Y

更新日期：2019-10-01 00:00:00

abstract：:alpha-SNAP is an essential component of the protein machinery responsible for membrane fusion events in different cell types. The hyh (hydrocephalus with hop gait) mouse carries a missense mutation in Napa gene that results in a point mutation (M105I) in alpha-SNAP protein. Homozygous animals for the mutant allele hav...

journal_title：Molecular and cellular probes

authors： Bátiz LF,Roales-Buján R,Rodríguez-Pérez LM,Matas IM,Páez P,Roque M,Jiménez AJ,Ramos C,Pérez-Fígares JM

更新日期：2009-12-01 00:00:00

abstract：:Infection of the cervix uteri with various types of human papillomaviruses is generally considered a necessary factor in the etiology of cancer of the cervix uteri. In many human populations throughout the world, approximately 90% of cervical carcinomas are found to harbour HPV genomes, as judged by Southern blot hybr...

journal_title：Molecular and cellular probes

authors： Chow V,Tham KM,Yeo-Gloss M,Lim-Tan SK,Sng I,Thirumoorthy T,Bernard HU

更新日期：1990-04-01 00:00:00

abstract：:The presence of human cytomegalovirus (HCMV) was tested in 388 cervicovaginal cells specimens obtained from the same number of pregnant women. HCMV was detected in 5.41%, 11.6% and 13.9% of these specimens by conventional culture, in situ DNA hybridization and polymerase chain reaction (PCR) methods, respectively. The...

journal_title：Molecular and cellular probes

authors： Yuan CF,Kao SM,Wang DC,Ng HT,Pao CC

更新日期：1990-12-01 00:00:00

abstract：:The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

journal_title：Molecular and cellular probes

authors： Chaubert P,Burri N,Cousin P,Shaw P

更新日期：2001-06-01 00:00:00

abstract：:An effective PCR-based genomic walking approach is described to discover previously unknown flanking genomic DNA sequences from Candidatus Liberibacter asiaticus, an unculturable, phloem-limited bacterium. Using this technique, 8564bp of new DNA sequences were obtained from three genomic loci; tufB-secE-nusG-rplKAJL-r...

journal_title：Molecular and cellular probes

authors： Lin H,Doddapaneni H,Bai X,Yao J,Zhao X,Civerolo EL

更新日期：2008-02-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was develop...

journal_title：Molecular and cellular probes

authors： Li R,Wang J,Liu L,Zhang R,Hao X,Han Q,Wang J,Yuan W

更新日期：2019-06-01 00:00:00

abstract：:This study aimed to examine the UBA6 role in brain injury mediated by acute cerebral infarction (ACI). In order to screen potential therapeutic targets for ACI, two expression profiles, including GSE97537 and GSE97533 datasets, were downloaded from the GEO database. The Venn method to identify the common DEGs. 68 up-r...

journal_title：Molecular and cellular probes

authors： Chen Z,Liu J,Chen Q,Su M,Lu H,Yang Y,Zhou G,Zhang X,Liu Y,Dong W,Fang Q

更新日期：2020-10-01 00:00:00

abstract：:The hydroxymethylbilane synthase (HMBS) mRNAs from 44 control individuals and 30 patients suffering from acute intermittent porphyria (AIP), were screened for length differences by reverse transcriptase polymerase chain reaction (RT-PCR) and any abnormalities were characterized by direct sequencing. Examination of the...

journal_title：Molecular and cellular probes

authors： Ong PM,Lanyon WG,Moore MR,Connor JM

更新日期：1998-04-01 00:00:00

abstract：:The pyrazinamidase gene coding for the enzyme that activates the bactericidal drug pyrazinamide contains a polymorphic site that is preserved in Mycobacterium bovis. We synthesized two sets of primers, one encompassing a 180 bp fragment, and the second spanning a 726 bp fragment including the full pncA gene. Following...

journal_title：Molecular and cellular probes

authors： Barouni AS,Augusto CJ,Lopes MT,Zanini MS,Salas CE

更新日期：2004-06-01 00:00:00

abstract：:In situ hybridization is the hybridization-mediated detection of specific nucleic acid sequences within structurally intact cells or tissues. As such it uniquely provides localization of nucleic acid superimposed on observable cellular and subcellular structural detail, allowing analysis unobtainable by other hybridiz...

journal_title：Molecular and cellular probes

authors： Moench TR

更新日期：1987-09-01 00:00:00

abstract：:Multiplex ligation dependent probe amplification (MLPA) assays were designed for the genes HEXB (OMIM: 606873), GM2A (OMIM: 613109) and SMARCAL1 (OMIM: 606622) of humans. Two sets of synthetic MLPA probes for these coding exons were tested. Changes in copy numbers were detected as well as single nucleotide polymorphis...

journal_title：Molecular and cellular probes

authors： Sobek AK,Evers C,Dekomien G

更新日期：2013-02-01 00:00:00

abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-rea...

journal_title：Molecular and cellular probes

authors： Chen HT,Zhang J,Ma YP,Ma LN,Ding YZ,Liu XT,Cai XP,Ma LQ,Zhang YG,Liu YS

更新日期：2010-04-01 00:00:00

abstract：:A DNA amplification procedure using heat stable Taq polymerase and the polymerase chain reaction is described for the detection of Pseudomonas aeruginosa in specimens from cystic fibrosis patients. A set of primers was selected on the basis of the nucleotide sequence of the algD gene encoding GDP mannose dehydrogenase...

journal_title：Molecular and cellular probes

authors： McIntosh I,Govan JR,Brock DJ

更新日期：1992-08-01 00:00:00

abstract：:Biotinylated single-stranded RNA probes from two of the eleven genome segments of the simian rotavirus SA11 were synthesized from cloned DNA and used in dot-blot and Northern-blot hybridization assays. Different types of membranes and conditions to prepare and use synthetic non-radioactive transcript probes were evalu...

journal_title：Molecular and cellular probes

authors： Bellinzoni R,Xi JA,Tanaka TN,Scodeller E,Estes MK

更新日期：1989-09-01 00:00:00

abstract：:Pre-mRNA processing factor 4 (PRPF4), a core protein in U4/U6 snRNP, maintains snRNP structures by interacting with PRPF3 and cyclophilin H. Expression of the PRPF4 gene affects cell survival as well as apoptosis and is responsible for retinitis pigmentosa (RP). Proteomics analysis shows that PRPF4 may be a therapeuti...

journal_title：Molecular and cellular probes

authors： Park S,Han SH,Kim HG,Jeong J,Choi M,Kim HY,Kim MG,Park JK,Han JE,Cho GJ,Kim MO,Ryoo ZY,Choi SK

更新日期：2019-10-01 00:00:00

abstract：:Although large expansions of the non-coding GGGGCC repeat in C9orf72 gene are clearly defined as pathogenic for Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Lobar Degeneration (FTLD), intermediate-length expansions have also been associated with those and other neurodegenerative diseases. Intermediate-length...

journal_title：Molecular and cellular probes

authors： Biasiotto G,Archetti S,Di Lorenzo D,Merola F,Paiardi G,Borroni B,Alberici A,Padovani A,Filosto M,Bonvicini C,Caimi L,Zanella I

更新日期：2017-04-01 00:00:00

abstract：:Advanced reflectance-based optical techniques for in vivo imaging often suffer from low contrast between neoplastic and normal tissue and are unable to image early biomolecular changes associated with carcinogenesis, thus limiting their clinical value. In this study, we exploit the resonance light scattering property ...

journal_title：Molecular and cellular probes

authors： Kah JC,Olivo MC,Lee CG,Sheppard CJ

更新日期：2008-02-01 00:00:00

abstract：:A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis. ...

journal_title：Molecular and cellular probes

authors： Ren H,Yang M,Zhang G,Liu S,Wang X,Ke Y,Du X,Wang Z,Huang L,Liu C,Chen Z

更新日期：2016-04-01 00:00:00

abstract：:The impact that next-generation sequencing technology (NGS) is having on many aspects of molecular and cell biology, is becoming increasingly apparent. One of the most noticeable outcomes of the new technology in human genetics, has been the accelerated rate of identification of disease-causing genes. Especially for r...

journal_title：Molecular and cellular probes

authors： Morris-Rosendahl DJ,Kaindl AM

更新日期：2015-10-01 00:00:00

abstract：:The present study evaluates the usefulness of a PCR-based method for routine paternity testing in 35 paternity cases. This identification method which is based on amplification of three hypervariable genetic loci, apoB, D1S80 and HLA-DQ alpha, is compared, with regard to reliability and technical feasibility, to the c...

journal_title：Molecular and cellular probes

authors： Helminen P,Sajantila A,Johnsson V,Lukka M,Ehnholm C,Peltonen L

更新日期：1992-02-01 00:00:00

abstract：:Metastasis and recurrence of breast cancer remain significant clinical problems. The expression level of CD44 protein is higher in breast cancer-initiating cancer stem cells; therefore, the early detection of CD44 using a sensitive diagnostic probe is important for breast cancer diagnosis and therapeutic purposes. In ...

journal_title：Molecular and cellular probes

authors： Cho JH,Lee SC,Ha NR,Lee SJ,Yoon MY

更新日期：2015-12-01 00:00:00

abstract：:Several systems for isolating viruses from environmental samples have been tested. The most promising method is based on genomic amplification. The authors attempted to detect adenovirus in nucleic-acid extracts from the Seine River estuary by a two-step amplification of a 220-bp segment of the conserved coding region...

journal_title：Molecular and cellular probes

authors： Castignolles N,Petit F,Mendel I,Simon L,Cattolico L,Buffet-Janvresse C

更新日期：1998-06-01 00:00:00

abstract：:Four isolates of Sarcocystis neurona from horses with equine protozoal myeloencephalitis and eight species of coccidia from the genera Sarcocystis, Toxoplasma or Eimeria were differentiated using the random amplified polymorphic DNA assay. A single, common, 550-bp DNA fragment was amplified from the DNA of each S. neu...

journal_title：Molecular and cellular probes

authors： Granstrom DE,MacPherson JM,Gajadhar AA,Dubey JP,Tramontin R,Stamper S

更新日期：1994-10-01 00:00:00

abstract：:Research has been focused on the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in pasteurized milk; however, pasteurized milk is a key ingredient in a variety of food products. Therefore, MAP contamination in milk-derived products must be investigated. We undertook a six-month study to investigate...

journal_title：Molecular and cellular probes

authors： Clark DL Jr,Anderson JL,Koziczkowski JJ,Ellingson JL

更新日期：2006-06-01 00:00:00

abstract：:This report describes detailed taxonomic and phylogenetic analysis of 15 non-tuberculous mycobacteria (NTMs) isolated from human pathological specimens in a Caribbean setting (12 slow-growers and three rapid-growers) that were not identified by cultural and biochemical tests and drug-susceptibility results. These isol...

journal_title：Molecular and cellular probes

authors： Ferdinand S,Legrand E,Goh KS,Berchel M,Mazzarelli G,Sola C,Tortoli E,Rastogi N

更新日期：2004-12-01 00:00:00

abstract：:Polymorphisms (rs1801282, rs8192678, rs7903146) of peroxisome proliferator-activated receptor gamma (PPARG), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PPARGC1A) and transcription factor 7-like 2 (TCF7L2) have recently been associated with different diseases, mainly type 2 diabetes. An assay...

journal_title：Molecular and cellular probes

authors： Habalová V,Klimcáková L,Zidzik J,Tkác I

更新日期：2009-02-01 00:00:00

abstract：:Clostridium septicum is a spore-forming anaerobe frequently implicated in cases of gangrenous dermatitis (GD) and other spontaneously occurring myonecrotic infections of poultry. Although C. septicum is readily cultured from diseased tissues it can be difficult to enumerate due to its tendency to swarm over the surfac...

journal_title：Molecular and cellular probes

authors： Neumann AP,Dunham SM,Rehberger TG,Siragusa GR

更新日期：2010-08-01 00:00:00

abstract：:Quantum dot (QD) molecular beacons were explored for sequence-specific DNA detection. The effectiveness of multiple linkage strategies and fluorescence quenchers were compared in hybridization-based assays. To compare linkage strategies, covalent amide linkage and streptavidin-biotin binding were used to link semicond...

journal_title：Molecular and cellular probes

authors： Cady NC,Strickland AD,Batt CA

更新日期：2007-04-01 00:00:00

abstract：:We investigated polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and cystathionine beta-synthase (CBS) genes in Korean patients with coronary artery disease (CAD) and identified a new polymorphism (c-->t) in intron 7 of the CBS gene using the single-strand conformation polymorphism method. No significant d...

journal_title：Molecular and cellular probes

authors： Hong SH,Song J,Kim JQ

更新日期：2001-04-01 00:00:00