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Detection of Mycobacterium avium subspecies paratuberculosis genetic components in retail cheese curds purchased in Wisconsin and Minnesota by PCR.

Abstract：

:Research has been focused on the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in pasteurized milk; however, pasteurized milk is a key ingredient in a variety of food products. Therefore, MAP contamination in milk-derived products must be investigated. We undertook a six-month study to investigate the presence of viable MAP and MAP genetic components in cheese curds purchased from retail outlets in the northern and southern regions of Wisconsin and Minnesota. A total of 98 retail cheese curd samples were tested for MAP by PCR prescreen, culture on Herrold's egg yolk agar slants with mycobactin J and amphoteracin B, naladixic acid, and vancomycin, and slant rinse PCR using IS900 and hspX primer sets. Although no viable MAP were able to be cultured, 5% of the samples were PCR positive with both the IS900 and hspX primer sets (MAP-specific DNA) when prescreened and 1% of the samples were PCR positive with both the IS900 and hspX primer sets when culture slants were rinsed and tested.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Clark DL Jr,Anderson JL,Koziczkowski JJ,Ellingson JL

doi

10.1016/j.mcp.2005.12.006

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

197-202

issue

3-4

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(06)00009-0

journal_volume

20

pub_type

杂志文章

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