Cell preservation methods and its application to studying rare disease.

abstract：:A polymerase chain reaction (PCR) method specific for Mycoplasma gallisepticum (MG) was evaluated. The PCR method was found to detect as few as two colour changing units (CCU) of MG and did not give false positive reactions with other avian mycoplasmas. In chickens inoculated with either MG or Mycoplasma synoviae (MS)...

journal_title：Molecular and cellular probes

authors： Slavik MF,Wang RF,Cao WW

更新日期：1993-12-01 00:00:00

abstract：:Human infection with the parasitic nematode Enterobius vermicularis occurs worldwide, particularly in children. Although its prevalence may exceed 35% in some parts of the world, molecular studies of E. vermicularis in humans are limited. The aim of the present study was to investigate the genetic variation within E. ...

journal_title：Molecular and cellular probes

authors： Piperaki ET,Spanakos G,Patsantara G,Vassalou E,Vakalis N,Tsakris A

更新日期：2011-04-01 00:00:00

abstract：PURPOSE:This study aimed to analyze the relationships of long non-coding RNAs (lncRNAs) and protein-coding genes in lung squamous cell carcinoma (LUSC). METHODS:RNA-seq data of LUSC deposited in the TCGA database were used to identify differentially expressed protein-coding genes (DECGs) and differentially expressed l...

journal_title：Molecular and cellular probes

authors： Wu X,Ruan L,Yang Y,Mei Q

更新日期：2016-06-01 00:00:00

abstract：:The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs i...

journal_title：Molecular and cellular probes

authors： Nagaraju J,Kathirvel M,Subbaiah EV,Muthulakshmi M,Kumar LD

更新日期：2002-02-01 00:00:00

abstract：:The flagellin genes of four Burkholderia pseudomallei and two Burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (PCR)-based isolation method using the same pair of primers. The PCR-amplification products of the isolates showed a single band of about 1.1 kb, which is similar to a type I...

journal_title：Molecular and cellular probes

authors： Tungpradabkul S,Wajanarogana S,Tunpiboonsak S,Panyim S

更新日期：1999-04-01 00:00:00

abstract：:Infection of the cervix uteri with various types of human papillomaviruses is generally considered a necessary factor in the etiology of cancer of the cervix uteri. In many human populations throughout the world, approximately 90% of cervical carcinomas are found to harbour HPV genomes, as judged by Southern blot hybr...

journal_title：Molecular and cellular probes

authors： Chow V,Tham KM,Yeo-Gloss M,Lim-Tan SK,Sng I,Thirumoorthy T,Bernard HU

更新日期：1990-04-01 00:00:00

abstract：:Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybri...

journal_title：Molecular and cellular probes

authors： Sakamoto H,Lemaire O,Merdinoglu D,Guesdon JL

更新日期：1989-06-01 00:00:00

abstract：:Species identification of Mycobacterium tuberculosis remains a cumbersome process. We have developed a simple method for treating clinical samples which permits direct polymerase chain reaction (PCR) amplification of mycobacterial target DNA without organic extraction. Samples were boiled for 30 min in TE-Triton, then...

journal_title：Molecular and cellular probes

authors： Sritharan V,Barker RH Jr

更新日期：1991-10-01 00:00:00

abstract：:Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten b...

journal_title：Molecular and cellular probes

authors： Liu CY,Song HQ,Zhang RL,Chen MX,Xu MJ,Ai L,Chen XG,Zhan XM,Liang SH,Yuan ZG,Lin RQ,Zhu XQ

更新日期：2011-08-01 00:00:00

abstract：:One pair of high-sensitive polymerase chain reaction (PCR) primers for Cryptosporidium parvum was constructed based on the sequence of random amplified polymorphic DNA. PCR with this primer pair amplified only the DNA of C. parvum, not the control DNA including Cryptosporidium muris. This primer pair had most advantag...

journal_title：Molecular and cellular probes

authors： Wu Z,Nagano I,Matsuo A,Uga S,Kimata I,Iseki M,Takahashi Y

更新日期：2000-02-01 00:00:00

abstract：:A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larva...

journal_title：Molecular and cellular probes

authors： Campbell BE,Rabelo EM,Hofmann A,Hu M,Gasser RB

更新日期：2010-08-01 00:00:00

abstract：:The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. conc...

journal_title：Molecular and cellular probes

authors： Bastyns K,Chapelle S,Vandamme P,Goossens H,De Wachter R

更新日期：1995-08-01 00:00:00

abstract：:Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a target DNA sequence. We performed SDA in the presence of a 5'-32P-oligodeoxynucleotide detector probe that contains a target binding sequence at its 3'-end and a recognition site for the restriction enzyme HincII at its 5'-end wh...

journal_title：Molecular and cellular probes

authors： Walker GT,Nadeau JG,Linn CP

更新日期：1995-12-01 00:00:00

abstract：AIM:Though Endostar (ES) could inhibit tumor growth by inhibiting tumor angiogenesis, other possible mechanisms have been less reported. This study aims to investigate the role of ES in the treatment of lung cancer from the perspective of macrophage-mediated epithelial mesenchymal transformation (EMT). METHODS:THP1 ce...

journal_title：Molecular and cellular probes

authors： Shen Y,Chen Q,Li L

更新日期：2019-06-01 00:00:00

abstract：:To find a specific method for HLA-B27 typing, we tested an HLA-B27-specific polymerase chain reaction. This method was used for screening 100 randomly selected blood donors, 10 of them being HLA-B27 positive. A flow cytometric method and this PCR method were compared. ...

journal_title：Molecular and cellular probes

authors： Lucotte G,Burckel A

更新日期：1997-08-01 00:00:00

abstract：:Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA...

journal_title：Molecular and cellular probes

authors： Axton RA,Brock DJ

更新日期：1994-06-01 00:00:00

abstract：:Long-term infection with high-risk HPV genotypes is the leading cause of cervical cancer. In the present study a Duplex Real-time PCR assay was developed in order to identify HPV types 16, 18, 31, 35, 51 and 66 in three reactions, through SYBR green I melting curve analysis. The method utilizes type-specific primer se...

journal_title：Molecular and cellular probes

authors： Tsakogiannis D,Papacharalampous M,Toska E,Kyriakopoulou Z,Dimitriou TG,Ruether IG,Komiotis D,Markoulatos P

更新日期：2015-02-01 00:00:00

abstract：:Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 196...

journal_title：Molecular and cellular probes

authors： Komissarov A,Fadeev A,Kosheleva A,Sintsova K,Grudinin M

更新日期：2017-10-01 00:00:00

abstract：:We describe a rapid, automated method for direct detection of known single-base changes in genomic DNA. Fluorescence-based DNA minisequence analysis is employed in a template-dependent reaction which involves a single nucleotide extension of an oligonucleotide primer by the correct fluorescently-tagged dideoxynucleoti...

journal_title：Molecular and cellular probes

authors： Kobayashi M,Rappaport E,Blasband A,Semeraro A,Sartore M,Surrey S,Fortina P

更新日期：1995-06-01 00:00:00

abstract：:A polymorphic GT dinucleotide repeat sequence has been identified in the 5' flanking region of the human growth hormone receptor (hGHR) gene on chromosome 5p13.1-p12, within the promoter region of the V9 5'UTR exon. Thirteen alleles have been identified in 50 non-related individuals, with an observed heterozygosity of...

journal_title：Molecular and cellular probes

authors： Hadjiyannakis S,Zheng H,Hendy GN,Goodyer CG

更新日期：2001-08-01 00:00:00

abstract：:Development of rapid amplification assays for the detection and identification of biological threat agents has become a focus of diagnostic efforts in recent years. The use of real-time PCR assays as diagnostic tools depends upon two critical processes. First, nucleic acid purification must provide template that is bo...

journal_title：Molecular and cellular probes

authors： Hartman LJ,Coyne SR,Norwood DA

更新日期：2005-02-01 00:00:00

abstract：:The present study evaluates the usefulness of a PCR-based method for routine paternity testing in 35 paternity cases. This identification method which is based on amplification of three hypervariable genetic loci, apoB, D1S80 and HLA-DQ alpha, is compared, with regard to reliability and technical feasibility, to the c...

journal_title：Molecular and cellular probes

authors： Helminen P,Sajantila A,Johnsson V,Lukka M,Ehnholm C,Peltonen L

更新日期：1992-02-01 00:00:00

abstract：:Although large expansions of the non-coding GGGGCC repeat in C9orf72 gene are clearly defined as pathogenic for Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Lobar Degeneration (FTLD), intermediate-length expansions have also been associated with those and other neurodegenerative diseases. Intermediate-length...

journal_title：Molecular and cellular probes

authors： Biasiotto G,Archetti S,Di Lorenzo D,Merola F,Paiardi G,Borroni B,Alberici A,Padovani A,Filosto M,Bonvicini C,Caimi L,Zanella I

更新日期：2017-04-01 00:00:00

abstract：:A rapid polymerase chain reaction (PCR) assay specific for Marek's Disease Virus (MDV) was developed. This assay was able to detect MDV in inoculated chick kidney cells at dilutions of 10(-5). Negative PCR results were obtained using uninoculated chick cells, Marek's Disease Vaccine (SB), Herpesvirus of Turkeys (HVT) ...

journal_title：Molecular and cellular probes

authors： Rong-Fu W,Beasley JN,Cao WW,Slavik MF,Johnson MG

更新日期：1993-04-01 00:00:00

abstract：:Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 's...

journal_title：Molecular and cellular probes

authors： Peruzzi B,Serra M,Pescucci C,Sica M,Lastraioli S,Rondelli T,Pedemonte S,Risitano AM,De Angioletti M,Piccioli P,Notaro R

更新日期：2010-12-01 00:00:00

abstract：:The single nucleotide polymorphism (SNP) genotyping is currently considered as a particularly valuable tool for the diagnosis of different pathologies. For this reason, over the past several years a great deal of effort has been devoted to developing accurate, rapid, and cost-effective technologies for SNP analysis. A...

journal_title：Molecular and cellular probes

authors： Etlik O,Koksal V,Arican-Baris ST,Baris I

更新日期：2011-08-01 00:00:00

abstract：:Surveillance for vancomycin resistant enterococci (VRE) by culture can be labour intensive and time consuming. We have developed a multiplex polymerase chain reaction (MPCR) which can be performed directly on the clinical specimen. The assay allows sensitive detection of enterococci with vanA - and vanB -mediated resi...

journal_title：Molecular and cellular probes

authors： Petrich AK,Luinstra KE,Groves D,Chernesky MA,Mahony JB

更新日期：1999-08-01 00:00:00

abstract：:Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficult. Reverse transcription plus polymerase chain reaction (RT-PCR) was ...

journal_title：Molecular and cellular probes

authors： Wang RF,Campbell WL,Cao WW,Colvert RM,Holland MA,Cerniglia CE

更新日期：1999-02-01 00:00:00

abstract：:Research has been focused on the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in pasteurized milk; however, pasteurized milk is a key ingredient in a variety of food products. Therefore, MAP contamination in milk-derived products must be investigated. We undertook a six-month study to investigate...

journal_title：Molecular and cellular probes

authors： Clark DL Jr,Anderson JL,Koziczkowski JJ,Ellingson JL

更新日期：2006-06-01 00:00:00

abstract：:Previous studies have demonstrated that insulin-like growth factor-I (IGF-1) and reactive oxygen species (ROS) are involved in the development and progression of various cancers. However, their regulatory mechanism remains unknown. In this study, we treated cancer cells (HeLa, HepG2 and SW1116 cells) and normal cells ...

journal_title：Molecular and cellular probes

authors： Wang C,An Y,Wang Y,Shen K,Wang X,Luan W,Ma F,Ni L,Liu M,Yu L

更新日期：2020-08-01 00:00:00