当前位置： SCI文献检索 > MOLECULAR AND CELLULAR PROBES期刊下所有文献 > Use of antibodies against the P36 protein of Mycoplasma hyopneumoniae for the identification of M. hyopneumoniae strains.

Use of antibodies against the P36 protein of Mycoplasma hyopneumoniae for the identification of M. hyopneumoniae strains.

Abstract：

:Mycoplasma hyopneumoniae, the principal aetiological agent of porcine enzootic pneumonia, synthesizes a 36 kDa protein (P36) which is an early and strong immunogenic factor in experimentally and naturally infected swine. Polyclonal antibodies were made against the recombinant P36 protein in rabbits and used for the identification of M. hyopneumoniae by the immunoblot technique. The proteins from the M. hyopneumoniae reference strains and from 13 M. hyopneumoniae field strains isolated from naturally infected pigs in Switzerland, Hungary, France and Canada were analysed by the immunoblot technique using anti-P36 antibodies. All 13 field strains and the three reference J strains of M. hyopneumoniae, received from different collections and laboratories, exhibited a strong reaction with a protein of 36 kDa indicating that the P36 protein is a common M. hyopneumoniae antigen. None of the different porcine Mycoplasma species including M. flocculare, M. hyorhinis, M. hyosynoviae, A. axanthum, A. laidlawii and A. granularum showed any reaction on the immunoblot with the anti-P36 antibodies. In addition, we have found no reaction with anti-P36 antibodies using 47 different Mycoplasma or Acholeplasma species isolated from human, mice, rat, poultry, ruminant, dog and cat. In conclusion we have shown that P36 is a protein that is a common antigen of M. hyopneumoniae strains and is not found in other Mycoplasma or Acholeplasma species tested. Because of its high specificity, P36 protein, or antibodies made against this protein can be used for the identification of M. hyopneumoniae strains.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Stipkovits L,Nicolet J,Haldimann A,Frey J

doi

10.1016/s0890-8508(05)80017-9

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

451-7

issue

6

eissn

0890-8508

issn

1096-1194

journal_volume

5

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR PROBES文献大全
  • Development of a novel and rapid polymerase spiral reaction (PSR) assay to detect Salmonella in pork and pork products.

    abstract：:The polymerase spiral reaction (PSR), a novel isothermal method for targeted DNA amplification, was effectively applied to detect Salmonella in artificially spiked pork. The specificity of the developed PSR was tested using 16 Salmonella and 15 non-Salmonella strains. The PSR assay was 10-fold more sensitive than conv...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101510

    authors： Momin KM,Milton AAP,Ghatak S,Thomas SC,Priya GB,Das S,Shakuntala I,Sanjukta R,Puro KU,Sen A

    更新日期：2020-04-01 00:00:00

  • Development of a TB green II-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine circovirus 2 and 3.

    abstract：:Porcine circovirus 3 (PCV3), as a newly emerged circovirus, is widely distributed in pig populations worldwide. Co-infection of PCV2 and PCV3 has been reported frequently in clinical samples. In the present study, a TB Green II-based duplex real-time polymerase chain reaction (qPCR) was developed to rapidly and differ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.04.001

    authors： Zhao Y,Han HY,Fan L,Tian RB,Cui JT,Li JY,Chen HY,Yang MF,Zheng LL

    更新日期：2019-06-01 00:00:00

  • Evaluation of the detection limits of PCR for identification of Mycoplasma pneumoniae in clinical samples.

    abstract：:The detection limits of the polymerase chain reaction (PCR) for Mycoplasma pneumoniae were determined using specimens from persons known to have had M. pneumoniae pneumonia. Four primers were selected from the known sequence of the P1 gene. The primer pair (P1-178 and P1-809) which generates a 631 fragment gave the lo...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1994.1017

    authors： Leng Z,Kenny GE,Roberts MC

    更新日期：1994-04-01 00:00:00

  • Genomic structure of the EPHA1 receptor tyrosine kinase gene.

    abstract：:Some receptor tyrosine kinase genes are mutated in inherited and somatically acquired human cancers. To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions. The gene contains 18 exons, two...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1999.0228

    authors： Owshalimpur D,Kelley MJ

    更新日期：1999-06-01 00:00:00

  • Rapid diagnosis of spinal muscular atrophy using tetra-primer ARMS PCR assay: simultaneous detection of SMN1 and SMN2 deletion.

    abstract：:Spinal muscular atrophy (SMA), the leading genetic cause of death in childhood, is an autosomal recessive neuromuscular disorder characterized by progressive muscle weakness, associated with deletions of the survival motor neuron 1 (SMN1) gene. Approximately 94% of SMA patients carry homologous deletions of SMN1 exon(...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2009.12.001

    authors： Baris I,Etlik O,Koksal V,Arican-Baris ST

    更新日期：2010-06-01 00:00:00

  • Direct detection of vanA and vanB genes in clinical specimens for rapid identification of vancomycin resistant enterococci (VRE) using multiplex PCR.

    abstract：:Surveillance for vancomycin resistant enterococci (VRE) by culture can be labour intensive and time consuming. We have developed a multiplex polymerase chain reaction (MPCR) which can be performed directly on the clinical specimen. The assay allows sensitive detection of enterococci with vanA - and vanB -mediated resi...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1999.0250

    authors： Petrich AK,Luinstra KE,Groves D,Chernesky MA,Mahony JB

    更新日期：1999-08-01 00:00:00

  • Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.

    abstract：:Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 's...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2010.07.002

    authors： Peruzzi B,Serra M,Pescucci C,Sica M,Lastraioli S,Rondelli T,Pedemonte S,Risitano AM,De Angioletti M,Piccioli P,Notaro R

    更新日期：2010-12-01 00:00:00

  • Diagnosis of mouse hepatitis virus contamination in mouse population by using nude mice and RT-PCR.

    abstract：:Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficult. Reverse transcription plus polymerase chain reaction (RT-PCR) was ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1998.0211

    authors： Wang RF,Campbell WL,Cao WW,Colvert RM,Holland MA,Cerniglia CE

    更新日期：1999-02-01 00:00:00

  • No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls.

    abstract：:New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2011.01.005

    authors： Sabunciyan S,Mandelberg N,Rabkin CS,Yolken R,Viscidi R

    更新日期：2011-04-01 00:00:00

  • Development and validation of a quantitative real time PCR assay for BK virus.

    abstract：:Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. We developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine. Consider...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2013.08.001

    authors： Mitui M,Leos NK,Lacey D,Doern C,Rogers BB,Park JY

    更新日期：2013-10-01 00:00:00

  • Detection of hepatitis B virus DNA in serum with nucleic acid probes labelled with 32P, biotin, alkaline phosphatase or sulphone.

    abstract：:To facilitate the clinical application of dot-blot hybridization for assaying hepatitis B virus (HBV) DNA, we compared the ability of nucleic acid probes labelled with 32P or with various non-radioactive markers to detect HBV DNA in patient serum. Cloned HBV DNA was hybridized with (1) 32P-labelled HBV DNA cloned in M...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(91)90053-m

    authors： Valentine-Thon E,Steinmann J,Arnold W

    更新日期：1991-08-01 00:00:00

  • Rapid PCR using nested primers of the 16S rRNA and the hippuricase (hip O) genes to detect Campylobacter jejuni and Campylobacter coli in environmental samples.

    abstract：:Identification of sources Campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. Detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.2002.0434

    authors： Bang DD,Wedderkopp A,Pedersen K,Madsen M

    更新日期：2002-10-01 00:00:00

  • Specific detection of enterovirus 71 directly from clinical specimens using real-time RT-PCR hybridization probe assay.

    abstract：:Enterovirus 71 (EV71) is one of the main causative agents of hand, foot and mouth disease (HFMD) in young children. Infections caused by EV71 could lead to many complications, ranging from brainstem encephalitis to pulmonary oedema, resulting in high mortality. Thus, rapid detection of the virus is required to enable ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2005.11.003

    authors： Tan EL,Chow VT,Kumarasinghe G,Lin RT,Mackay IM,Sloots TP,Poh CL

    更新日期：2006-04-01 00:00:00

  • Rotavirus gene detection with biotinylated single-stranded RNA probes.

    abstract：:Biotinylated single-stranded RNA probes from two of the eleven genome segments of the simian rotavirus SA11 were synthesized from cloned DNA and used in dot-blot and Northern-blot hybridization assays. Different types of membranes and conditions to prepare and use synthetic non-radioactive transcript probes were evalu...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(89)90004-2

    authors： Bellinzoni R,Xi JA,Tanaka TN,Scodeller E,Estes MK

    更新日期：1989-09-01 00:00:00

  • Up-regulated miR-374a-3p relieves lipopolysaccharides induced injury in CHON-001 cells via regulating Wingless-type MMTV integration site family member 5B.

    abstract：BACKGROUND:Osteoarthritis (OA) is a frequent and incurable joint disease, inducing significant pain and seriously threatening to human health. It has been reported that microRNAs (miRNAs) play crucial roles on cancers and inflammatory diseases via cooperating with genes. However, the effect of miR-374a-3p/Wingless-type...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101541

    authors： Shi FL,Ren LX

    更新日期：2020-06-01 00:00:00

  • Class D and E tetracycline resistance determinants in gram-negative bacteria from catfish ponds.

    abstract：:DNA probes were used to examine tetracycline-resistant Gram-negative bacteria (281 strains representing eight species) from catfish ponds. The isolates, which did not previously hybridize with the Tet A, B and C determinants, were examined for the presence of tetracycline-resistance Tet D and Tet E determinants. The d...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/s0890-8508(95)91572-9

    authors： DePaola A,Roberts MC

    更新日期：1995-10-01 00:00:00

  • Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis.

    abstract：:Beak atrophy and dwarfism syndrome (BADS) is commonly caused by co-infection with duck circovirus (DuCV) and novel goose parvovirus (NGPV). Therefore, concurrent detection of both viruses is important for monitoring and limiting BADS, although such a diagnostic test has not been reported. In this study, we developed a...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101648

    authors： Wang Y,Cui Y,Li Y,Jiang S,Liu H,Wang J,Li Y

    更新日期：2020-10-01 00:00:00

  • Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples.

    abstract：:A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis. ...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2016.02.007

    authors： Ren H,Yang M,Zhang G,Liu S,Wang X,Ke Y,Du X,Wang Z,Huang L,Liu C,Chen Z

    更新日期：2016-04-01 00:00:00

  • A simple procedure to differentiate ailA and ailNA gene variants among human pathogenic Yersinia enterocolitica strains.

    abstract：:The identification and differentiation of the two variants of the ail gene, ailA from the more virulent American serotypes (08, 013a, 13b, 018, 020, 021) and ailNA from the less virulent non-American serotypes (03, 04, 05, 06, 09, 027 and 07, 8) was studied in a panel of 32 Yersinia enterocolitica human pathogenic iso...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1994.1025

    authors： Sayada C,Picard B,Elion J,Krishnamoorthy R

    更新日期：1994-06-01 00:00:00

  • Polymorphism rs3737787 of Upstream Stimulatory Factor 1 gene is associated with serum lipid phenotype in Nigerian population.

    abstract：:Serum lipid profile which is determined by genotype-phenotype relationship plays a significant role in the development of cardiovascular disease. Upstream stimulatory factor 1 (USF1), has been reported to be associated with serum lipid levels in different population, hence, this study investigated the association of v...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2020.101687

    authors： Onadeko OT,Okunowo WO,Imaga NOA,Abdulrazaq MM,Onuminya OJ,Van-Lare TO,Nwosu M

    更新日期：2020-12-08 00:00:00

  • Characterization of a Caenorhabditis elegans glc seven-like phosphatase (gsp) orthologue from Haemonchus contortus (Nematoda).

    abstract：:A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larva...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2010.02.001

    authors： Campbell BE,Rabelo EM,Hofmann A,Hu M,Gasser RB

    更新日期：2010-08-01 00:00:00

  • A pncA polymorphism to differentiate between Mycobacterium bovis and Mycobacterium tuberculosis.

    abstract：:The pyrazinamidase gene coding for the enzyme that activates the bactericidal drug pyrazinamide contains a polymorphic site that is preserved in Mycobacterium bovis. We synthesized two sets of primers, one encompassing a 180 bp fragment, and the second spanning a 726 bp fragment including the full pncA gene. Following...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2003.11.006

    authors： Barouni AS,Augusto CJ,Lopes MT,Zanini MS,Salas CE

    更新日期：2004-06-01 00:00:00

  • Quantitative PCR evaluation of deletions/duplications identified by array CGH.

    abstract：:Genomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentia...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.101421

    authors： Baldan F,Passon N,Burra S,Demori E,Russo PD,Damante G

    更新日期：2019-08-01 00:00:00

  • Multiple paternity and sperm storage in captive Hermann's tortoises, Testudo hermanni boettgeri determined from amniotic fluid adhering to the eggshell.

    abstract：:We identified multiple paternity in 52.9% of the clutches of Hermann's tortoise Testudo hermanni boettgeri using polymorphic microsatellite markers. In addition we demonstrated sperm storage across seasons. DNA was extracted from the amniotic fluid adhering to the eggshell's inner surface, a procedure suitable for eas...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2015.05.009

    authors： Farke CM,Olek K,Gerding WM,Distler C

    更新日期：2015-08-01 00:00:00

  • Rapid differentiation of citrus Hop stunt viroid variants by real-time RT-PCR and high resolution melting analysis.

    abstract：:The RNA genome of pathogenic and non-pathogenic variants of citrus Hop stunt viroid (HSVd) differ by five to six nucleotides located within the variable (V) domain referred to as the "cachexia expression motif". Sensitive hosts such as mandarin and its hybrids are seriously affected by cachexia disease. Current method...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2013.07.003

    authors： Loconsole G,Onelge N,Yokomi RK,Kubaa RA,Savino V,Saponari M

    更新日期：2013-10-01 00:00:00

  • Rapid identification of Renibacterium salmoninarum using an oligonucleotide probe complementary to 16S rRNA.

    abstract：:Bacterial kidney disease in salmonid fish is caused by the slow-growing Gram-positive rod, Renibacterium salmoninarum. The partial sequence of 16S rRNA from R. salmoninarum was determined and compared with published bacterial 16S rRNA sequences. From this sequence information, a 30-bases-long oligonucleotide was desig...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1006/mcpr.1993.1004

    authors： Mattsson JG,Gersdorf H,Jansson E,Hongslo T,Göbel UB,Johansson KE

    更新日期：1993-02-01 00:00:00

  • Identification of crucial regulatory relationships between long non-coding RNAs and protein-coding genes in lung squamous cell carcinoma.

    abstract：PURPOSE:This study aimed to analyze the relationships of long non-coding RNAs (lncRNAs) and protein-coding genes in lung squamous cell carcinoma (LUSC). METHODS:RNA-seq data of LUSC deposited in the TCGA database were used to identify differentially expressed protein-coding genes (DECGs) and differentially expressed l...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2016.02.009

    authors： Wu X,Ruan L,Yang Y,Mei Q

    更新日期：2016-06-01 00:00:00

  • Direct detection of Actinobacillus pleuropneumoniae in swine lungs and tonsils by real-time recombinase polymerase amplification assay.

    abstract：:Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was develop...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2019.03.007

    authors： Li R,Wang J,Liu L,Zhang R,Hao X,Han Q,Wang J,Yuan W

    更新日期：2019-06-01 00:00:00

  • Molecular diagnosis of genital HPV DNA types by polymerase chain reaction and sensitivity-standardized filter in situ hybridization in randomly sampled cohorts of Singapore women.

    abstract：:Infection of the cervix uteri with various types of human papillomaviruses is generally considered a necessary factor in the etiology of cancer of the cervix uteri. In many human populations throughout the world, approximately 90% of cervical carcinomas are found to harbour HPV genomes, as judged by Southern blot hybr...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/0890-8508(90)90013-p

    authors： Chow V,Tham KM,Yeo-Gloss M,Lim-Tan SK,Sng I,Thirumoorthy T,Bernard HU

    更新日期：1990-04-01 00:00:00

  • Development of a p28-based PCR assay for Ehrlichia chaffeensis.

    abstract：:Detection of Ehrlichia chaffeensis is necessary to study interactions between the parasite and its vertebrate and invertebrate hosts. The purpose of this study was to develop a sensitive, specific PCR assay for E. chaffeensis based on the outer membrane protein gene, p28. Candidate primer sets were identified and rank...

    journal_title：Molecular and cellular probes

    pub_type： 杂志文章

    doi：10.1016/j.mcp.2003.10.002

    authors： Wagner ER,Bremer WG,Rikihisa Y,Ewing SA,Needham GR,Unver A,Wang X,Stich RW

    更新日期：2004-04-01 00:00:00